NOTE] Comparative Assessment of the Intracellular Survival of the Burkholderia pseudomallei bopC Mutant

Varintip Srinon; Sunsiree Muangman; Nithima Imyaem; Veerachat Muangsombut; Natalie R. Lazar Adler; Edouard E. Galyov; Sunee Korbsrisate

doi:10.1007/s12275-013-2557-3

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Research Support, Non-U.S. Gov't
NOTE] Comparative Assessment of the Intracellular Survival of the Burkholderia pseudomallei bopC Mutant: Varintip Srinon ¹, Sunsiree Muangman ¹, Nithima Imyaem ¹, Veerachat Muangsombut ¹, Natalie R. Lazar Adler ², Edouard E. Galyov ², Sunee Korbsrisate ¹; Journal of Microbiology 2013;51(4):522-526.
DOI: https://doi.org/10.1007/s12275-013-2557-3
Published online: August 30, 2013

¹Department of Immunology, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok 10700, Thailand, ²Department of Infection, Immunity and Inflammation, University of Leicester, Leicester LE1 9HN, United Kingdom

Corresponding author: Sunee Korbsrisate , Tel: +662-419-6636,

Received: 15 October 2012 • Accepted: 16 March 2013

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Abstract

Burkholderia pseudomallei, the causative agent of melioidosis, is a Gram-negative saprophytic bacterium capable of surviving within phagocytic cells. To assess the role of BopC (a type III secreted effector protein) in the pathogenesis of B. pseudomallei, a B. pseudomallei bopC mutant was used to infect J774A.1 macrophage-like cells. The bopC mutant showed significantly reduced intracellular survival in infected macrophages compared to wild-type B. pseudomallei. In addition, the bopC mutant displayed delayed escape from endocytic vesicles compared with the wild-type strain. This indicates that BopC is important, and at least in part, needed for intracellular survival of B. pseudomallei.

Keywords: Burkholderia pseudomallei;BopC;type III secretion effector;intracellular survival;phagocyte

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NOTE] Comparative Assessment of the Intracellular Survival of the Burkholderia pseudomallei bopC Mutant

J. Microbiol. 2013;51(4):522-526. Published online August 30, 2013

DOI: https://doi.org/10.1007/s12275-013-2557-3

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