A Novel Retron of Vibrio parahaemolyticus Is Closely Related to Retron-Vc95 of Vibrio cholerae

Toshi Shimamoto; Ashraf M. Ahmed; Tadashi Shimamoto

doi:10.1007/s12275-013-2715-7

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A Novel Retron of Vibrio parahaemolyticus Is Closely Related to Retron-Vc95 of Vibrio cholerae: Toshi Shimamoto ¹, Ashraf M. Ahmed ², Tadashi Shimamoto ¹; Journal of Microbiology 2013;51(3):323-328.
DOI: https://doi.org/10.1007/s12275-013-2715-7
Published online: June 28, 2013

¹Laboratory of Food Microbiology and Hygiene, Graduate School of Biosphere Science, Hiroshima University, Higashi-Hiroshima 739-8528, Japan, ²Department of Bacteriology, Mycology and Immunology, Faculty of Veterinary Medicine, Kafrelsheikh University, Kafr El-Sheikh 33516, Egypt¹Laboratory of Food Microbiology and Hygiene, Graduate School of Biosphere Science, Hiroshima University, Higashi-Hiroshima 739-8528, Japan, ²Department of Bacteriology, Mycology and Immunology, Faculty of Veterinary Medicine, Kafrelsheikh University, Kafr El-Sheikh 33516, Egypt

Corresponding author: Tadashi Shimamoto , Tel: +81-82-424-7897,

Received: 31 December 2012 • Accepted: 17 February 2013

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Abstract

Some bacteria produce a satellite RNA-DNA complex termed msDNA, multicopy single-stranded DNA. In this report, msDNA from Vibrio parahaemolyticus, a cause of acute gastroenteritis, was identified and named msDNA-Vp96. The retron element containing the ret gene, encoding the reverse transcriptase (RT) that is responsible for msDNA production, was cloned and characterized. Comparison of msDNAVp96 and msDNA-Vc95, from Vibrio cholerae, showed a high level of sequence similarity. We exchanged the two ret genes to examine whether msDNA was produced by the RT from different sources. We found that RT-Vp96 of V. parahaemolyticus was able to synthesize msDNA-Vc95 of V. cholerae and vice versa. To the best of our knowledge, this is the first report that RT from different bacterial species can synthesize msDNA.

Keywords: reverse transcriptase;msDNA;pathogenic bacteria;vibrios

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Retrons and their applications in genome engineering
Anna J Simon, Andrew D Ellington, Ilya J Finkelstein
Nucleic Acids Research.2019; 47(21): 11007. CrossRef
An Unexplored Diversity of Reverse Transcriptases in Bacteria
Steven Zimmerly, Li Wu, Alan Lambowitz, Nancy Craig
Microbiology Spectrum.2015;[Epub] CrossRef

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A Novel Retron of Vibrio parahaemolyticus Is Closely Related to Retron-Vc95 of Vibrio cholerae

J. Microbiol. 2013;51(3):323-328. Published online June 28, 2013

DOI: https://doi.org/10.1007/s12275-013-2715-7

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