Involvement of Alternative Oxidase in the Regulation of Sensitivity of Sclerotinia sclerotiorum to the Fungicides Azoxystrobin and Procymidone

Ting Xu; Ya-Ting Wang; Wu-Sheng Liang; Fei Yao; Yong-Hong Li; Dian-Rong Li; Hao Wang; Zheng-Yi Wang

doi:10.1007/s12275-013-2534-x

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Involvement of Alternative Oxidase in the Regulation of Sensitivity of Sclerotinia sclerotiorum to the Fungicides Azoxystrobin and Procymidone: Ting Xu ^1,2, Ya-Ting Wang ^1,2, Wu-Sheng Liang ^1,2, Fei Yao ^1,2, Yong-Hong Li ³, Dian-Rong Li ³, Hao Wang ³, Zheng-Yi Wang ^1,2; Journal of Microbiology 2013;51(3):352-358.
DOI: https://doi.org/10.1007/s12275-013-2534-x
Published online: April 26, 2013

¹Institute of Biotechnology, College of Agriculture and Biotechnology, Zijingang Campus, Zhejiang University, Hangzhou 310058, P. R. China, ²State Key Laboratory of Rice Biology, Hangzhou 310058, P. R. China, ³Hybrid Rapeseed Research Center of Shaanxi Province, Dali 715105, P. R. China¹Institute of Biotechnology, College of Agriculture and Biotechnology, Zijingang Campus, Zhejiang University, Hangzhou 310058, P. R. China, ²State Key Laboratory of Rice Biology, Hangzhou 310058, P. R. China, ³Hybrid Rapeseed Research Center of Shaanxi Province, Dali 715105, P. R. China

Corresponding author: Wu-Sheng Liang , Tel: +86-571-88982261,

Received: 4 October 2012 • Accepted: 17 January 2013

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Abstract

Sclerotinia sclerotiorum is a filamentous fungal pathogen that can infect many economically important crops and vegetables. Alternative oxidase is the terminal oxidase of the alternative respiratory pathway in fungal mitochondria. The function of alternative oxidase was investigated in the regulation of sensitivity of S. sclerotiorum to two commercial fungicides, azoxystrobin and procymidone which have different fungitoxic mechanisms. Two isolates of S. sclerotiorum were sensitive to both fungicides. Application of salicylhydroxamic acid, a specific inhibitor of alternative oxidase, significantly increased the values of effective concentration causing 50% mycelial growth inhibition (EC50) of azoxystrobin to both S. sclerotiorum isolates, whereas notably decreased the EC50 values of procymidone. In mycelial respiration assay azoxystrobin displayed immediate inhibitory effect on cytochrome pathway capacity, but had no immediate effect on alternative pathway capacity. In contrast, procymidone showed no immediate impact on capacities of both cytochrome and alternative pathways in the mycelia. However, alternative oxidase encoding gene (aox) transcript and protein levels, alternative respiration pathway capacity of the mycelia were obviously increased by pre-treatment for 24 h with both azoxystrobin and procymidone. These results indicate that alternative oxidase was involved in the regulation of sensitivity of S. sclerotiorum to the fungicides azoxystrobin and procymidone, and that both fungicides could affect aox gene expression and the alternative respiration pathway capacity development in mycelia of this fungal pathogen.

Keywords: Sclerotinia sclerotiorum;alternative oxidase;mitochondrial respiration;azoxystrobin;procymidone;fungicide sensitivity

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Involvement of Alternative Oxidase in the Regulation of Sensitivity of Sclerotinia sclerotiorum to the Fungicides Azoxystrobin and Procymidone

J. Microbiol. 2013;51(3):352-358. Published online April 26, 2013

DOI: https://doi.org/10.1007/s12275-013-2534-x

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