The α-Barrel Tip Region of Escherichia coli TolC Homologs of Vibrio vulnificus Interacts with the MacA Protein to Form the Functional Macrolide-Specific Efflux Pump MacAB-TolC

Minho Lee; Hyun-Lee Kim; Saemee Song; Minju Joo; Seunghwa Lee; Daeyoung Kim; Yoonsoo Hahn; Nam-Chul Ha; Kangseok Lee

doi:10.1007/s12275-013-2699-3

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The α-Barrel Tip Region of Escherichia coli TolC Homologs of Vibrio vulnificus Interacts with the MacA Protein to Form the Functional Macrolide-Specific Efflux Pump MacAB-TolC: Minho Lee ¹, Hyun-Lee Kim ¹, Saemee Song ¹, Minju Joo ¹, Seunghwa Lee ¹, Daeyoung Kim ¹, Yoonsoo Hahn ¹, Nam-Chul Ha ², Kangseok Lee ¹; Journal of Microbiology 2013;51(2):154-159.
DOI: https://doi.org/10.1007/s12275-013-2699-3
Published online: April 27, 2013

¹Department of Life Science, Chung-Ang University, Seoul 156-756, Republic of Korea, ²Department of Manufacturing Pharmacy, Pusan National University, Busan 609-735, Republic of Korea

Corresponding author: Kangseok Lee , Tel: +82-2-822-5241,

Received: 20 December 2012 • Accepted: 25 December 2012

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Abstract

TolC and its homologous family of proteins are outer membrane factors that are essential for exporting small molecules and toxins across the outer membrane in Gram-negative bacteria. Two open reading frames in the Vibrio vulnificus genome that encode proteins homologous to Escherichia coli TolC, designated TolCV1 and TolCV2, have 51.3% and 29.6% amino acid identity to TolC, respectively. In this study, we show that TolCV1 and TolCV2 functionally and physically interacted with the membrane fusion protein, MacA, a component of the macrolide-specific MacAB-TolC pump of E. coli. We further show that the conserved residues located at the aperture tip region of the α-hairpin of TolCV1 and TolCV2 played an essential role in the formation of the functional MacAB-TolC pump using site-directed mutational analyses. Our findings suggest that these outer membrane factors have conserved tip-to-tip interaction with the MacA membrane fusion protein for action of the drug efflux pump in Gramnegative bacteria.

Keywords: MacA;MacB;TolC;TolCV1;TolCV2;Type I secretion system

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The α-Barrel Tip Region of Escherichia coli TolC Homologs of Vibrio vulnificus Interacts with the MacA Protein to Form the Functional Macrolide-Specific Efflux Pump MacAB-TolC

J. Microbiol. 2013;51(2):154-159. Published online April 27, 2013

DOI: https://doi.org/10.1007/s12275-013-2699-3

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