Construction of a Streptomyces lydicus A01 Transformant with a chit42 Gene from Trichoderma harzianum P1 and Evaluation of Its Biocontrol Activity against Botrytis cinerea

Qiong Wu; Linquan Bai; Weicheng Liu; Yingying Li; Caige Lu; Yaqian Li; Kehe Fu; Chuanjin Yu; Jie Chen

doi:10.1007/s12275-013-2321-8

Articles

Page Path: HOME > J. Microbiol > Volume 51(2); 2013 > Article

Research Support, Non-U.S. Gov't
Construction of a Streptomyces lydicus A01 Transformant with a chit42 Gene from Trichoderma harzianum P1 and Evaluation of Its Biocontrol Activity against Botrytis cinerea: Qiong Wu ¹, Linquan Bai ², Weicheng Liu ³, Yingying Li ¹, Caige Lu ³, Yaqian Li ¹, Kehe Fu ¹, Chuanjin Yu ¹, Jie Chen ¹; Journal of Microbiology 2013;51(2):166-173.
DOI: https://doi.org/10.1007/s12275-013-2321-8
Published online: April 27, 2013

¹School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai 200240, P. R. China, ²School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai 200030, P. R. China, ³Institute of Plant and Environment Protection, Beijing Academy of Agriculture and Forestry Sciences, Beijing 100097, P. R. China

Corresponding author: Jie Chen , Tel: +86-21-34206141,

Received: 21 June 2012 • Accepted: 13 November 2012

prev next

73 Views
0 Download
12 Crossref
17 Scopus

Full Article

Abstract

Streptomyces lydicus A01 and Trichoderma harzianum P1 are potential biocontrol agents of fungal diseases in plants. S. lydicus A01 produces natamycin to bind the ergosterol of the fungal cell membrane and inhibits the growth of Botrytis cinerea. T. harzianum P1, on the other hand, features high chitinase activity and decomposes the chitin in the cell wall of B. cinerea. To obtain the synergistic biocontrol effects of chitinase and natamycin on Botrytis cinerea, this study transformed the chit42 gene from T. harzianum P1 to S. lydicus A01. The conjugal transformant (CT) of S. lydicus A01 with the chit42 gene was detected using polymerase chain reaction (PCR). Associated chitinase activity and natamycin production were examined using the 3, 5-dinitrosalicylic acid (DNS) method and ultraviolet spectrophotometry, respectively. The S. lydicus A01-chit42 CT showed substantially higher chitinase activity and natamycin production than its wild type strain (WT). Consequently, the biocontrol effects of S. lydicus A01-chit42 CT on B. cinerea, including inhibition to spore germination and mycelial growth, were highly improved compared with those of the WT. Our research indicates that the biocontrol effect of Streptomyces can be highly improved by transforming the exogenous resistance gene, i.e. chit42 from Trichoderma, which not only enhances the production of antibiotics, but also provides a supplementary function by degrading the cell walls of the pathogens.

Keywords: Streptomyces lydicus A01;chit42 from Trichoderma harzianum P1;Botrytis cinerea;chitinase activity;natamycin production

Figure & Data

References

Citations

Citations to this article as recorded by

Strain improvement of Trichoderma harzianum for enhanced biocontrol capacity: Strategies and prospects
Ziyang Xiao, Qinqin Zhao, Wei Li, Liwei Gao, Guodong Liu
Frontiers in Microbiology.2023;[Epub] CrossRef
Novel mechanism of hydrogen peroxide for promoting efficient natamycin synthesis in Streptomyces
Gongli Zong, Guangxiang Cao, Jiafang Fu, Peipei Zhang, Xi Chen, Wenxiu Yan, Lulu Xin, Zhongxue Wang, Yan Xu, Rongzhen Zhang, Beile Gao
Microbiology Spectrum.2023;[Epub] CrossRef
Biological Control of Tomato Gray Mold Caused by Botrytis Cinerea with the Entomopathogenic Fungus Metarhizium Anisopliae
Most.Sinthia Sarven, Qiuyan Hao, Junbo Deng, Fang Yang, Gaofeng Wang, Yannong Xiao, Xueqiong Xiao
Pathogens.2020; 9(3): 213. CrossRef
Improving performance of microbial biocontrol agents against plant diseases
Malek Marian, Masafumi Shimizu
Journal of General Plant Pathology.2019; 85(5): 329. CrossRef
Identification of a novel strain, Streptomyces blastmyceticus JZB130180, and evaluation of its biocontrol efficacy against Monilinia fructicola
Mi Ni, Qiong Wu, Hong-li Wang, Wei-cheng Liu, Bin Hu, Dian-peng Zhang, Juan Zhao, De-wen Liu, Cai-ge Lu
Journal of Zhejiang University-SCIENCE B.2019; 20(1): 84. CrossRef
Antifungal, Plant Growth-Promoting, and Genomic Properties of an Endophytic Actinobacterium Streptomyces sp. NEAU-S7GS2
Dongli Liu, Rui Yan, Yansong Fu, Xiangjing Wang, Ji Zhang, Wensheng Xiang
Frontiers in Microbiology.2019;[Epub] CrossRef
The Endochitinase of Clonostachysrosea Expression in Bacillus amyloliquefaciens Enhances the Botrytis cinerea Resistance of Tomato
Yangyang Zheng, Xudong Wang, Siyuan Liu, Kewei Zhang, Zhibo Cai, Xiuling Chen, Yao Zhang, Jiayin Liu, Aoxue Wang
International Journal of Molecular Sciences.2018; 19(8): 2221. CrossRef
Omics for understanding synergistic action of validamycin A and Trichoderma asperellum GDFS1009 against maize sheath blight pathogen
Qiong Wu, Lida Zhang, Hai Xia, Chuanjin Yu, Kai Dou, Yaqian Li, Jie Chen
Scientific Reports.2017;[Epub] CrossRef
Complete genome sequencing and antibiotics biosynthesis pathways analysis of Streptomyces lydicus 103
Nan Jia, Ming-Zhu Ding, Hao Luo, Feng Gao, Ying-Jin Yuan
Scientific Reports.2017;[Epub] CrossRef
Biological control of tomato gray mold caused by Botrytis cinerea by using Streptomyces spp.
Sawai Boukaew, Poonsuk Prasertsan, Claire Troulet, Marc Bardin
BioControl.2017; 62(6): 793. CrossRef
Heterologous coexpression of Vitreoscilla hemoglobin and Bacillus megaterium glucanase in Streptomyces lydicus A02 enhanced its production of antifungal metabolites
Huiling Wu, Jinjin Li, Dan Dong, Ting Liu, Taotao Zhang, Dianpeng Zhang, Weicheng Liu
Enzyme and Microbial Technology.2015; 81: 80. CrossRef
Expression of Paenibacillus polymyxa β-1,3-1,4-glucanase in Streptomyces lydicus A01 improves its biocontrol effect against Botrytis cinerea
Jinjin Li, Weicheng Liu, Lijin Luo, Dan Dong, Ting Liu, Taotao Zhang, Caige Lu, Dewen Liu, Dianpeng Zhang, Huiling Wu
Biological Control.2015; 90: 141. CrossRef

Cite this Article

Cite this Article: export Copy Download Format; Close

Download Citation

Download a citation file in RIS format that can be imported by all major citation management software, including EndNote, ProCite, RefWorks, and Reference Manager.

Format:

RIS — For EndNote, ProCite, RefWorks, and most other reference management software
BibTeX — For JabRef, BibDesk, and other BibTeX-specific software

Include:

Citation for the content below
Citation and abstract for the content below

Construction of a Streptomyces lydicus A01 Transformant with a chit42 Gene from Trichoderma harzianum P1 and Evaluation of Its Biocontrol Activity against Botrytis cinerea

J. Microbiol. 2013;51(2):166-173. Published online April 27, 2013

DOI: https://doi.org/10.1007/s12275-013-2321-8

Related articles