Development of SCAR Primers Based on a Repetitive DNA Fingerprint for Escherichia coli Detection

Aphidech Sangdee; Sitakan Natphosuk; Adunwit Srisathan; Kusavadee Sangdee

doi:10.1007/s12275-013-2244-4

Articles

Page Path: HOME > J. Microbiol > Volume 51(1); 2013 > Article

Research Support, Non-U.S. Gov't
Development of SCAR Primers Based on a Repetitive DNA Fingerprint for Escherichia coli Detection: Aphidech Sangdee ¹, Sitakan Natphosuk ¹, Adunwit Srisathan ¹, Kusavadee Sangdee ²; Journal of Microbiology 2013;51(1):31-35.
DOI: https://doi.org/10.1007/s12275-013-2244-4
Published online: March 2, 2013

¹Department of Biology, Faculty of Science, Mahasarakham University, 44150, Thailand, ²Preclinical Division, Faculty of Medicine, Mahasarakham University, 44150, Thailand

Corresponding author: Aphidech Sangdee , Tel: +6643754245,

Received: 8 May 2012 • Accepted: 8 October 2012

prev next

184 Views
0 Download
3 Crossref
5 Scopus

Full Article

Download PDF

Abstract

The present study aimed to use enterobacterial repetitive intergenic consensus (ERIC) fingerprints to design SCAR primers for the detection of Escherichia coli. The E. coli strains were isolated from various water sources. The primary presumptive identification of E. coli was achieved using MacConkey agar. Nineteen isolates were selected and confirmed to be E. coli strains based on seven biochemical characteristics. ERIC-PCR with ERIC 1R and ERIC 2 primers were used to generate DNA fingerprints. ERIC-PCR DNA profiles showed variant DNA profiles among the tested E. coli strains and distinguished all E. coli strains from the other tested bacterial strains. A 350 bp band that predominated in five E. coli strains was used for the development of the species-specific SCAR primers EC-F1 and EC-R1. The primers showed good specificity for E. coli, with the exception of a single false positive reaction with Sh. flexneri DMST 4423. The primers were able to detect 50 pg and 100 CFU/ml of genomic DNA and cells of E. coli, respectively.

Keywords: E. coli;ERIC-PCR;SCAR primer;detection

Figure & Data

References

Citations

Citations to this article as recorded by

Development of seven novel specific SCAR markers for rapid identification of Phytophthora sojae: the cause of root- and stem-rot disease of soybean
Qin Xiong, Jing Xu, Xinyue Zheng, Yu Zhu, Chen Zhang, Xiaoli Wang, Xiaobo Zheng, Yuanchao Wang
European Journal of Plant Pathology.2019; 153(2): 517. CrossRef
Development of SCAR markers for rapid and specific detection of Pseudomonas syringae pv. morsprunorum races 1 and 2, using conventional and real-time PCR
Monika Kałużna, Pedro Albuquerque, Fernando Tavares, Piotr Sobiczewski, Joanna Puławska
Applied Microbiology and Biotechnology.2016; 100(8): 3693. CrossRef
Genetic Diversity of Food-Isolated Salmonella Strains through Pulsed Field Gel Electrophoresis (PFGE) and Enterobacterial Repetitive Intergenic Consensus (ERIC-PCR)
Imen Fendri, Amal Ben Hassena, Noel Grosset, Mohamed Barkallah, Lamia Khannous, Victoria Chuat, Michel Gautier, Radhouane Gdoura, A. Mark Ibekwe
PLoS ONE.2013; 8(12): e81315. CrossRef

Cite this Article

Cite this Article: export Copy Download Format; Close

Download Citation

Download a citation file in RIS format that can be imported by all major citation management software, including EndNote, ProCite, RefWorks, and Reference Manager.

Format:

RIS — For EndNote, ProCite, RefWorks, and most other reference management software
BibTeX — For JabRef, BibDesk, and other BibTeX-specific software

Include:

Citation for the content below
Citation and abstract for the content below

Development of SCAR Primers Based on a Repetitive DNA Fingerprint for Escherichia coli Detection

J. Microbiol. 2013;51(1):31-35. Published online March 2, 2013

DOI: https://doi.org/10.1007/s12275-013-2244-4

Related articles