Hrq1 Functions Independently of Sgs1 to Preserve Genome Integrity in Saccharomyces cerevisiae

Do-Hee Choi; Rina Lee; Sung-Hun Kwon; Sung-Ho Bae

doi:10.1007/s12275-013-3048-2

Articles

Page Path: HOME > J. Microbiol > Volume 51(1); 2013 > Article

Research Support, Non-U.S. Gov't
Hrq1 Functions Independently of Sgs1 to Preserve Genome Integrity in Saccharomyces cerevisiae: Do-Hee Choi , Rina Lee , Sung-Hun Kwon , Sung-Ho Bae; Journal of Microbiology 2013;51(1):105-112.
DOI: https://doi.org/10.1007/s12275-013-3048-2
Published online: March 2, 2013

Department of Biological Sciences, College of Natural Science, Inha University, Incheon 402-751, Republic of KoreaDepartment of Biological Sciences, College of Natural Science, Inha University, Incheon 402-751, Republic of Korea

Corresponding author: Sung-Ho Bae , Tel: +82-32-860-7712,

Received: 21 January 2013 • Accepted: 29 January 2013

prev next

43 Views
0 Download
18 Crossref
20 Scopus

Full Article

Abstract

Maintenance of genome stability in eukaryotes involves a number of conserved proteins, including RecQ helicases, which play multiple roles at various steps in homologous recombination and DNA repair pathways. Sgs1 has been described as the only RecQ helicase in lower eukaryotes. However, recent studies revealed the presence of a second RecQ helicase, Hrq1, which is most homologous to human RECQL4. Here we show that hrq1Δ mutation resulted in increased mitotic recombination and spontaneous mutation in Saccharomyces cerevisiae, and sgs1Δ mutation had additive effects on the phenotypes of hrq1Δ. We also observed that the hrq1Δ mutant was sensitive to 4-nitroquinoline 1-oxide and cisplatin, which was not complemented by overexpression of Sgs1. In addition, the hrq1Δ sgs1Δ double mutant displayed synthetic growth defect as well as a shortened chronological life span compared with the respective single mutants. Analysis of the type of age-dependent Canr mutations revealed that only point mutations were found in hrq1Δ, whereas significant numbers of gross deletion mutations were found in sgs1Δ. Our results suggest that Hrq1 is involved in recombination and DNA repair pathways in S. cerevisiae independent of Sgs1.

Keywords: Hrq1;RecQ helicase;DNA helicase;RECQL4 orthologue;Sgs1;recombination

Figure & Data

References

Citations

Citations to this article as recorded by

Deletion of IRC19 Causes Defects in DNA Double-Strand Break Repair Pathways in Saccharomyces cerevisiae
Ju-Hee Choi, Oyungoo Bayarmagnai, Sung-Ho Bae
Journal of Microbiology.2024; 62(9): 749. CrossRef
Systematic analysis of changes across different developmental stages of the mushroom Sarcomyxa edulis
Chao Duan, Lan Yao, Jian-Hua Lv, Chuan-Wen Jia, Feng-hua Tian, Chang-Tian Li
Gene.2022; 824: 146450. CrossRef
Human RecQ Helicases in DNA Double-Strand Break Repair
Huiming Lu, Anthony J. Davis
Frontiers in Cell and Developmental Biology.2021;[Epub] CrossRef
Effects of the loss of mismatch repair genes on single-strand annealing between divergent sequences in Saccharomyces cerevisiae
Ye-Seul Lim, Ju-Hee Choi, Kyu-Jin Ahn, Min-Ku Kim, Sung-Ho Bae
Journal of Microbiology.2021; 59(4): 401. CrossRef
Analyses of DNA double-strand break repair pathways in tandem arrays of HXT genes of Saccharomyces cerevisiae
Ju-Hee Choi, Ye-Seul Lim, Min-Ku Kim, Sung-Ho Bae
Journal of Microbiology.2020; 58(11): 957. CrossRef
The Genetic and Physical Interactomes of theSaccharomyces cerevisiaeHrq1 Helicase
Cody M Rogers, Elsbeth Sanders, Phoebe A Nguyen, Whitney Smith-Kinnaman, Amber L Mosley, Matthew L Bochman
G3 Genes|Genomes|Genetics.2020; 10(12): 4347. CrossRef
Maintenance of Yeast Genome Integrity by RecQ Family DNA Helicases
Sonia Vidushi Gupta, Kristina Hildegard Schmidt
Genes.2020; 11(2): 205. CrossRef
The nature of meiotic chromosome dynamics and recombination in budding yeast
Soogil Hong, Jeong Hwan Joo, Hyeseon Yun, Keunpil Kim
Journal of Microbiology.2019; 57(4): 221. CrossRef
Ku complex suppresses recombination in the absence of MRX activity during budding yeast meiosis
Hyeseon Yun, Keunpil Kim
BMB Reports.2019; 52(10): 607. CrossRef
The RecQ‐like helicase HRQ1 is involved in DNA crosslink repair in Arabidopsis in a common pathway with the Fanconi anemia‐associated nuclease FAN1 and the postreplicative repair ATPase RAD5A
Sarah Röhrig, Annika Dorn, Janina Enderle, Angelina Schindele, Natalie J. Herrmann, Alexander Knoll, Holger Puchta
New Phytologist.2018; 218(4): 1478. CrossRef
Disruption of SUMO-targeted ubiquitin ligases Slx5–Slx8/RNF4 alters RecQ-like helicase Sgs1/BLM localization in yeast and human cells
Stefanie Böhm, Michael Joseph Mihalevic, Morgan Alexandra Casal, Kara Anne Bernstein
DNA Repair.2015; 26: 1. CrossRef
Interaction of RECQ4 and MCM10 is important for efficient DNA replication origin firing in human cells
Maciej Kliszczak, Hana Sedlackova, Ganesha P. Pitchai, Werner W. Streicher, Lumir Krejci, Ian D. Hickson
Oncotarget.2015; 6(38): 40464. CrossRef
Hrq1 facilitates nucleotide excision repair of DNA damage induced by 4-nitroquinoline-1-oxide and cisplatin in Saccharomyces cerevisiae
Do-Hee Choi, Moon-Hee Min, Min-Ji Kim, Rina Lee, Sung-Hun Kwon, Sung-Ho Bae
Journal of Microbiology.2014; 52(4): 292. CrossRef
Conditional Genetic Interactions ofRTT107,SLX4, andHRQ1Reveal Dynamic Networks upon DNA Damage inS. cerevisiae
Grace P Leung, Maria J Aristizabal, Nevan J Krogan, Michael S Kobor
G3 Genes|Genomes|Genetics.2014; 4(6): 1059. CrossRef
Characterization of the Caenorhabditis elegans HIM-6/BLM Helicase: Unwinding Recombination Intermediates
Hana Jung, Jin A Lee, Seoyoon Choi, Hyunwoo Lee, Byungchan Ahn, Michael Lichten
PLoS ONE.2014; 9(7): e102402. CrossRef
Characterization of Hrq1-Rad14 Interaction in Saccharomyces cerevisiae
Moon-Hee Min, Min-Ji Kim, You-Jin Choi, Min-Ju You, Uy-Ra Kim, Hyo-Bin An, Chae-Hyun Kim, Chae-Yeon Kwon, Sung-Ho Bae
The Korean Journal of Microbiology.2014; 50(2): 95. CrossRef
The intrinsically disordered amino-terminal region of human RecQL4: multiple DNA-binding domains confer annealing, strand exchange and G4 DNA binding
Heidi Keller, Kristin Kiosze, Juliane Sachsenweger, Sebastian Haumann, Oliver Ohlenschläger, Tarmo Nuutinen, Juhani E. Syväoja, Matthias Görlach, Frank Grosse, Helmut Pospiech
Nucleic Acids Research.2014; 42(20): 12614. CrossRef
Human RecQ Helicases in DNA Repair, Recombination, and Replication
Deborah L. Croteau, Venkateswarlu Popuri, Patricia L. Opresko, Vilhelm A. Bohr
Annual Review of Biochemistry.2014; 83(1): 519. CrossRef

Cite this Article

Cite this Article: export Copy Download Format; Close

Download Citation

Download a citation file in RIS format that can be imported by all major citation management software, including EndNote, ProCite, RefWorks, and Reference Manager.

Format:

RIS — For EndNote, ProCite, RefWorks, and most other reference management software
BibTeX — For JabRef, BibDesk, and other BibTeX-specific software

Include:

Citation for the content below
Citation and abstract for the content below

Hrq1 Functions Independently of Sgs1 to Preserve Genome Integrity in Saccharomyces cerevisiae

J. Microbiol. 2013;51(1):105-112. Published online March 2, 2013

DOI: https://doi.org/10.1007/s12275-013-3048-2

Related articles