NOTE] Mutations Upregulating the flhDC Operon of Escherichia coli K-12

Changhan Lee; Chankyu Park

doi:10.1007/s12275-013-2212-z

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NOTE] Mutations Upregulating the flhDC Operon of Escherichia coli K-12: Changhan Lee , Chankyu Park; Journal of Microbiology 2013;51(1):140-144.
DOI: https://doi.org/10.1007/s12275-013-2212-z
Published online: March 2, 2013

Department of Life Sciences, Korea Advanced Institute of Science and Technology, Daejeon 305-701, Republic of Korea

Corresponding author: Chankyu Park , Tel: +82-42-350-2629,

Received: 19 April 2012 • Accepted: 8 October 2012

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Abstract

Bacterial motility is governed by the flhDC master operon that is under the control of factors like OmpR, LrhA, HdfR, and H-NS. Previously, derivatives of the wild-type MG1655 strain of E. coli K-12 with enhanced motility were found to contain insertion sequences (ISs) in the regulatory region of the flhDC operon. Here, we report that not only integrations of IS insertion sequences into the regulatory region of the flhDC operon, but also a missense mutation in the lrhA gene enhances motility by relieving transcriptional repression of the flhDC operon. Two novel IS insertions were found upstream of flhDC. So far, the relationships between the transacting factors and the cis-acting regulatory sequences associated with the flhDC operon have not been clearly established. In this study, it was found that effects of the cis- and trans-acting mutations were acting in parallel, suggesting their apparently independent regulation of flagellar expression.

Keywords: flhDC;lrhA;insertion sequence;motility

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NOTE] Mutations Upregulating the flhDC Operon of Escherichia coli K-12

J. Microbiol. 2013;51(1):140-144. Published online March 2, 2013

DOI: https://doi.org/10.1007/s12275-013-2212-z

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