Molecular Serotyping of Salmonella enterica by Complete rpoB Gene Sequencing

Won-Jin Seong; Hyuk-Joon Kwon; Tae-Eun Kim; Deog-Yong Lee; Mi-Sun Park; Jae-Hong Kim

doi:10.1007/s12275-012-2547-x

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Research Support, Non-U.S. Gov't
Molecular Serotyping of Salmonella enterica by Complete rpoB Gene Sequencing: Won-Jin Seong ¹, Hyuk-Joon Kwon ², Tae-Eun Kim ³, Deog-Yong Lee ⁴, Mi-Sun Park ⁴, Jae-Hong Kim ^1,2; Journal of Microbiology 2012;50(6):962-969.
DOI: https://doi.org/10.1007/s12275-012-2547-x
Published online: December 30, 2012

¹Laboratory of Avian Diseases, College of Veterinary Medicine and BK21 for Veterinary Science, Seoul National University, Seoul 151-742, Republic of Korea, ²Zoonotic Disease Institute (ZooDI), College of Veterinary Medicine and BK21 for Veterinary Science, Seoul National University, Seoul 151-742, Republic of Korea, ³BioPOA Co., Yongin 446-599, Republic of Korea, ⁴Division of Enteric Bacterial Infections, Center for Infectious Diseases, Korea Centers for Disease Control and Prevention, Osong 363-951, Republic of Korea

Corresponding author: Jae-Hong Kim , Tel: +82-2-880-1250,

Received: 8 October 2012 • Accepted: 26 November 2012

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Abstract

Serotyping has been the gold standard for identifying Salmonella, but it requires large amounts of standard antisera. Multilocus sequence typing (MLST) has been applied to identify Salmonella serovars, but the recombination of 4–7 housekeeping genes and multiple analytic steps diminish its applicability. In the present study, we determined the complete sequences of the RNA polymerase beta subunit gene (rpoB) and 7 housekeeping genes (aroC, dnaN, hemD, hisD, purE, sucA, and thrA) for 76 strains of 33 Salmonella enterica serovars and conducted phylogenetic analyses together with the corresponding gene sequences of 24 reference strains registered in the GenBank database. Based on the phylogenetic analyses, 100 strains from 40 serovars and 91 strains from 37 serovars were classified into 60 rpoB (RST) and 49 multilocus sequence types (ST), respectively. The nucleotide similarities were 98.8–100% and 96.9–100% for the complete rpoB gene and the seven concatenated housekeeping genes, respectively. The strains of 35 and 30 serovars formed serovar-specific branches or clusters in the rpoB and housekeeping gene phylogenetic trees, respectively. Therefore, complete rpoB gene sequencing and phylogenetic analysis may be a useful method for identifying Salmonella serovars that is a simpler, more cost-effective, and less time-consuming alternative or complementary method to MLST and conventional serotyping.

Keywords: Salmonella;serotyping;rpoB;MLST;phylogenetic analysis

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Molecular Serotyping of Salmonella enterica by Complete rpoB Gene Sequencing

J. Microbiol. 2012;50(6):962-969. Published online December 30, 2012

DOI: https://doi.org/10.1007/s12275-012-2547-x

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