Enhancement of Butanol Tolerance and Butanol Yield in Clostridium acetobutylicum Mutant NT642 Obtained by Nitrogen Ion Beam Implantation

Xiao-Bo Liu; Qiu-Ya Gu; Xiao-Bin Yu; Wei Luo

doi:10.1007/s12275-012-2289-9

Articles

Page Path: HOME > J. Microbiol > Volume 50(6); 2012 > Article

Research Support, Non-U.S. Gov't
Enhancement of Butanol Tolerance and Butanol Yield in Clostridium acetobutylicum Mutant NT642 Obtained by Nitrogen Ion Beam Implantation: Xiao-Bo Liu , Qiu-Ya Gu , Xiao-Bin Yu , Wei Luo; Journal of Microbiology 2012;50(6):1024-1028.
DOI: https://doi.org/10.1007/s12275-012-2289-9
Published online: December 30, 2012

The Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi 214122, Jiangsu, P. R. China

Corresponding author: Xiao-Bin Yu , Tel: +86-0510-85918167,

Received: 29 May 2012 • Accepted: 27 July 2012

prev next

61 Views
0 Download
24 Crossref
29 Scopus

Full Article

Abstract

As a promising alternative biofuel, biobutanol can be produced through acetone/butanol/ethanol (ABE) fermentation. Currently, ABE fermentation is still a small-scale industry due to its low production and high input cost. Moreover, butanol toxicity to the Clostridium fermentation host limits the accumulation of butanol in the fermentation broth. The wild-type Clostridium acetobutylicum D64 can only produce about 13 g butanol/L and tolerates less than 2% (v/v) butanol. To improve the tolerance of C. acetobutylicum D64 for enhancing the production of butanol, nitrogen ion beam implantation was employed and finally five mutants with enhanced butanol tolerance were obtained. Among these, the most butanol tolerant mutant C. acetobutylicum NT642 can tolerate above 3% (v/v) butanol while the wide-type strain can only withstand 2% (v/v). In batch fermentation, the production of butanol and ABE yield of C. acetobutylicum NT642 was 15.4 g/L and 22.3 g/L, respectively, which were both higher than those of its parental strain and the other mutants using corn or cassava as substrate. Enhancing butanol tolerance is a great precondition for obtaining a hyperyield producer. Nitrogen ion beam implantation could be a promising biotechnology to improve butanol tolerance and production of the host strain C. acetobutylicum.

Keywords: butanol tolerance;butanol yield;Clostridium acetobutylicum;nitrogen ion beam implantation;fermentation

Figure & Data

References

Citations

Citations to this article as recorded by

The potential of native and engineered Clostridia for biomass biorefining
Paola Ponsetto, Emilia Malgorzata Sasal, Roberto Mazzoli, Francesca Valetti, Gianfranco Gilardi
Frontiers in Bioengineering and Biotechnology.2024;[Epub] CrossRef
Mathematical modeling of fermentation from glucose, xylose, and food waste of clostridia sp. strain BOH3 for the production of ABE solvents and hydrogen
Elie R. Chalhoub, Joanne M. Belovich
Results in Engineering.2024; 22: 102366. CrossRef
Biobutanol production from underutilized substrates using Clostridium: Unlocking untapped potential for sustainable energy development
Devina Syifa Nabila, Rosamond Chan, Rizky Riscahya Pratama Syamsuri, Puspita Nurlilasari, Wan Abd Al Qadr Imad Wan-Mohtar, Abdullah Bilal Ozturk, Nia Rossiana, Febri Doni
Current Research in Microbial Sciences.2024; 7: 100250. CrossRef
Current progress on engineering microbial strains and consortia for production of cellulosic butanol through consolidated bioprocessing
Angela Re, Roberto Mazzoli
Microbial Biotechnology.2023; 16(2): 238. CrossRef
A critical review on biofilm-based reactor systems for enhanced syngas fermentation processes
Burcu Gunes
Renewable and Sustainable Energy Reviews.2021; 143: 110950. CrossRef
Proteomic Analysis Identifies Dysregulated Proteins in Butanol-Tolerant Gram-Positive Lactobacillus mucosae BR0713–33
Siqing Liu, Nasib Qureshi, Kenneth Bischoff, Costel C. Darie
ACS Omega.2021; 6(5): 4034. CrossRef
How to outwit nature: Omics insight into butanol tolerance
Alexander Arsov, Kaloyan Petrov, Penka Petrova
Biotechnology Advances.2021; 46: 107658. CrossRef
Role of efflux in enhancing butanol tolerance of bacteria
Maryna Vasylkivska, Petra Patakova
Journal of Biotechnology.2020; 320: 17. CrossRef
Effect of fermented sausages with probiotic Enterococcus faecium CRL 183 on gut microbiota using dynamic colonic model
Mariana Nougalli Roselino, Isabel Kimiko Sakamoto, Maria Angela Tallarico Adorno, Josiane Maria Márcia Canaan, Graciela Font de Valdez, Elizeu Antonio Rossi, Katia Sivieri, Daniela Cardoso Umbelino Cavallini
LWT.2020; 132: 109876. CrossRef
Enhancement of Butanol Production: From Biocatalysis to Bioelectrocatalysis
Xiaobo Liu, Xiaobin Yu
ACS Energy Letters.2020; 5(3): 867. CrossRef
“Saddle-shaped” dose-survival effect, is it a general and valuable phenomenon in microbes in response to heavy ion beam irradiation?
Xiaopeng Guo, Miaomiao Zhang, Yue Gao, Wenjian Li, Dong Lu
Annals of Microbiology.2019; 69(3): 221. CrossRef
A genome-wide view of mutations in respiration-deficient mutants of Saccharomyces cerevisiae selected following carbon ion beam irradiation
Xiaopeng Guo, Miaomiao Zhang, Yue Gao, Guozhen Cao, Yang Yang, Dong Lu, Wenjian Li
Applied Microbiology and Biotechnology.2019; 103(4): 1851. CrossRef
In situ biobutanol recovery from clostridial fermentations: a critical review
Pablo Jiménez-Bonilla, Yi Wang
Critical Reviews in Biotechnology.2018; 38(3): 469. CrossRef
Microalgae for biobutanol production – Technology evaluation and value proposition
Tong Kai Yeong, Kailin Jiao, Xianhai Zeng, Lu Lin, Sharadwata Pan, Michael K. Danquah
Algal Research.2018; 31: 367. CrossRef
Genomic, Transcriptional, and Phenotypic Analysis of the Glucose Derepressed Clostridium beijerinckii Mutant Exhibiting Acid Crash Phenotype
Seung‐Oh Seo, Holger Janssen, Andrew Magis, Yi Wang, Ting Lu, Nathan D. Price, Yong‐Su Jin, Hans P. Blaschek
Biotechnology Journal.2017;[Epub] CrossRef
Progress and perspectives on improving butanol tolerance
Siqing Liu, Nasib Qureshi, Stephen R. Hughes
World Journal of Microbiology and Biotechnology.2017;[Epub] CrossRef
Genetic Engineering In BioButanol Production And Tolerance
Ashok Rao, A. Sathiavelu, S. Mythili
Brazilian Archives of Biology and Technology.2016;[Epub] CrossRef
Ex situ product recovery for enhanced butanol production by Clostridium beijerinckii
Sang-Hyun Lee, Moon-Ho Eom, Jin-Dal-Rae Choi, Sooah Kim, Jungyeon Kim, Yong-An Shin, Kyoung Heon Kim
Bioprocess and Biosystems Engineering.2016; 39(5): 695. CrossRef
n-butanol: challenges and solutions for shifting natural metabolic pathways into a viable microbial production
Paola Branduardi, Danilo Porro, Michael Sauer
FEMS Microbiology Letters.2016; 363(8): fnw070. CrossRef
High-Level Butanol Production from Cassava Starch by a Newly Isolated Clostridium acetobutylicum
Shubo Li, Yuan Guo, Fuzhi Lu, Jiajian Huang, Zongwen Pang
Applied Biochemistry and Biotechnology.2015; 177(4): 831. CrossRef
Metabolic engineering of Clostridium acetobutylicum for butyric acid production with high butyric acid selectivity
Yu-Sin Jang, Jung Ae Im, So Young Choi, Jung Im Lee, Sang Yup Lee
Metabolic Engineering.2014; 23: 165. CrossRef
Application of new metabolic engineering tools for Clostridium acetobutylicum
Tina Lütke-Eversloh
Applied Microbiology and Biotechnology.2014; 98(13): 5823. CrossRef
Integrated butanol recovery for an advanced biofuel: current state and prospects
Chuang Xue, Jing-Bo Zhao, Li-Jie Chen, Feng-Wu Bai, Shang-Tian Yang, Jian-Xin Sun
Applied Microbiology and Biotechnology.2014; 98(8): 3463. CrossRef
Enhancing butanol tolerance and preventing degeneration in Clostridium acetobutylicum by 1-butanol–glycerol storage during long-term preservation
Xiaobo Liu, Qiuya Gu, Chunli Liao, Xiaobin Yu
Biomass and Bioenergy.2014; 69: 192. CrossRef

Cite this Article

Cite this Article: export Copy Download Format; Close

Download Citation

Download a citation file in RIS format that can be imported by all major citation management software, including EndNote, ProCite, RefWorks, and Reference Manager.

Format:

RIS — For EndNote, ProCite, RefWorks, and most other reference management software
BibTeX — For JabRef, BibDesk, and other BibTeX-specific software

Include:

Citation for the content below
Citation and abstract for the content below

Enhancement of Butanol Tolerance and Butanol Yield in Clostridium acetobutylicum Mutant NT642 Obtained by Nitrogen Ion Beam Implantation

J. Microbiol. 2012;50(6):1024-1028. Published online December 30, 2012

DOI: https://doi.org/10.1007/s12275-012-2289-9

Related articles