Characterization of Thermostable Deblocking Aminopeptidases of Archaeon Thermococcus onnurineus NA1 by Proteomic and Biochemical Approaches

Yeol Gyun Lee; Sun-Hee Leem; Young-Ho Chung; Seung Il Kim

doi:10.1007/s12275-012-2461-2

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Characterization of Thermostable Deblocking Aminopeptidases of Archaeon Thermococcus onnurineus NA1 by Proteomic and Biochemical Approaches: Yeol Gyun Lee ¹, Sun-Hee Leem ², Young-Ho Chung ¹, Seung Il Kim ¹; Journal of Microbiology 2012;50(5):792-797.
DOI: https://doi.org/10.1007/s12275-012-2461-2
Published online: November 4, 2012

¹Division of Life Science, Korea Basic Science Institute, Daejeon 305-333, Republic of Korea, ²Department of Biology, Dong-A University, Busan 602-715, Republic of Korea¹Division of Life Science, Korea Basic Science Institute, Daejeon 305-333, Republic of Korea, ²Department of Biology, Dong-A University, Busan 602-715, Republic of Korea

Corresponding author: Young-Ho Chung , Tel: +82-42-865-3451,
Seung Il Kim , Tel: +82-42-865-3451,

Received: 30 August 2012 • Accepted: 9 October 2012

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Abstract

Thermococcus onnurineus NA1 is a hyperthermophilic archaeon that grows optimally at >80°C. The deblocking aminopeptidase (DAP) (TNA1-DAP1) encoded in Ton_1032 of T. onnurineus NA1 is considered a major DAP. However, four genes encoding putative DAP have been identified from a genomic analysis of T. onnurineus NA1. A proteomic analysis revealed that all four DAPs were differentially induced in YPS culture medium and, particularly, two DAPs (TNA1-DAP1 and TNA1-DAP2) were dominantly expressed in T. onnurineus NA1. The biochemical properties and enzyme activity of DAPs induced in an E. coli expression system suggested that the two major DAPs play complementary roles in T. onnurineus NA1.

Keywords: Thermococcus onnurineus NA1;deblocking aminopeptidases;proteomic identification

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Characterization of Thermostable Deblocking Aminopeptidases of Archaeon Thermococcus onnurineus NA1 by Proteomic and Biochemical Approaches

J. Microbiol. 2012;50(5):792-797. Published online November 4, 2012

DOI: https://doi.org/10.1007/s12275-012-2461-2

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