Establishment and Characterization of the Epithelioma Papulosum Cyprini (EPC) Cell Line Persistently Infected with Infectious Pancreatic Necrosis Virus (IPNV), an Aquabirnavirus

Hyoung Jun Kim; Jae-Kwon Cho; Hyung-Kyu Hwang; Myung-Joo Oh; Toyohiko Nishizawa

doi:10.1007/s12275-012-2364-2

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Establishment and Characterization of the Epithelioma Papulosum Cyprini (EPC) Cell Line Persistently Infected with Infectious Pancreatic Necrosis Virus (IPNV), an Aquabirnavirus: Hyoung Jun Kim ¹, Jae-Kwon Cho ², Hyung-Kyu Hwang ², Myung-Joo Oh ³, Toyohiko Nishizawa ³; Journal of Microbiology 2012;50(5):821-826.
DOI: https://doi.org/10.1007/s12275-012-2364-2
Published online: November 4, 2012

¹Animal, Plant and Fisheries Quarantine and Inspection Agency, Jungbu Regional Office, Incheon 400-800, Republic of Korea, ²Southwest Sea Fisheries Research Institute, National Fisheries Research & Development Institute, Yeosu 556-823, Republic of Korea, ³Department of Aqualife Medicine, Chonnam National University, Yeosu 550-749, Republic of Korea

Corresponding author: Toyohiko Nishizawa , Tel: +82-61-659-7178,

Received: 17 July 2012 • Accepted: 28 September 2012

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Abstract

Infectious pancreatic necrosis virus (IPNV), a type species of aquabirnaviruses in the family Birnaviridae, is an etiological agent of infectious pancreatic necrosis and has been isolated from epizootics of cultured salmonids. In the present study, an epithelioma papulosum cyprini (EPC) cell line persistently infected with IPNV (PI-EPC) was experimentally established by subculturing EPC cells surviving IPNV infection, and was characterized. PI-EPC cells were morphologically indistinguishable from EPC, but continued to grow and yield IPNV. PI-EPC cells showed no cytopathic effect due to IPNV inoculation, and susceptibility of PI-EPC cells against heterologous viruses was not different from that of EPC cells. Only one cell of 103.5 PI-EPC cells produced IPNV at approximately 100.5 50% tissue culture infectious dose (TCID50)/cell/day, which was approximately 1,000 times lower than that of normal EPC cells. PI-EPC cells that did not yield IPNV (N-PI-EPC) were screened. The IPNV genome was detected from both PI-EPC and N-PI-EPC cells, and the IPNV VP2 structural protein was detected from both cell lines, but no other IPNV proteins were observed by Western blot analysis with anti-IPNV serum. Thus, multiplication of IPNV in PI-EPC cells was regulated by some host cell factors, except interferon.

Keywords: aquabirnavirus;infectious pancreatic necrosis virus;IPNV;persistent infection;virus productivity;EPC

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Establishment and Characterization of the Epithelioma Papulosum Cyprini (EPC) Cell Line Persistently Infected with Infectious Pancreatic Necrosis Virus (IPNV), an Aquabirnavirus

J. Microbiol. 2012;50(5):821-826. Published online November 4, 2012

DOI: https://doi.org/10.1007/s12275-012-2364-2

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