Identification of Chaperones in Freeze Tolerance in Saccharomyces cerevisiae

Mahendran Chinnamara Naicker; I Seul Jo; Hana Im

doi:10.1007/s12275-012-2411-z

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Identification of Chaperones in Freeze Tolerance in Saccharomyces cerevisiae: Mahendran Chinnamara Naicker , I Seul Jo , Hana Im; Journal of Microbiology 2012;50(5):882-887.
DOI: https://doi.org/10.1007/s12275-012-2411-z
Published online: November 4, 2012

Department of Molecular Biology, Sejong University, Seoul 143-747, Republic of Korea

Corresponding author: Hana Im , Tel: +82-2-3408-3643,

Received: 3 August 2012 • Accepted: 18 September 2012

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Abstract

Exposure to low temperatures reduces protein folding rates and induces the cold denaturation of proteins. Considering the roles played by chaperones in facilitating protein folding and preventing protein aggregation, chaperones must exist that confer tolerance to cold stress. Here, yeast strains lacking individual chaperones were screened for reduced freezing tolerance. In total, 19 of 82 chaperone-deleted strains tested were more sensitive to freeze-thaw treatment than wild-type cells. The reintroduction of the respective chaperone genes into the deletion mutants recovered the freeze tolerance. The freeze sensitivity of the chaperone-knockout strains was also retained in the presence of 20% glycerol.

Keywords: chaperone;yeast;freeze tolerance;Saccharomyces cerevisiae;cold shock response

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Systematic Characterization and Analysis of the Freeze–Thaw Tolerance Gene Set in the Budding Yeast, Saccharomyces cerevisiae
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International Journal of Molecular Sciences.2025; 26(5): 2149. CrossRef
Enhancing Freezing Stress Tolerance through Regulation of the Ubiquitin–Proteasome System in Saccharomyces cerevisiae
Ryoya Tanahashi, Akira Nishimura, Kyoyuki Kan, Natsumi Ishizaki, Shiho Fujishima, Hisanori Endo, Hiroshi Takagi
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Micromanaging freeze tolerance: the biogenesis and regulation of neuroprotective microRNAs in frozen brains
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Molecular Physiology of Freeze Tolerance in Vertebrates
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Physiological Reviews.2017; 97(2): 623. CrossRef
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Bulletin of the Korean Chemical Society.2016; 37(3): 366. CrossRef
Molecular Biology of Freezing Tolerance
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Comprehensive Physiology.2013; 3(3): 1283. CrossRef

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Identification of Chaperones in Freeze Tolerance in Saccharomyces cerevisiae

J. Microbiol. 2012;50(5):882-887. Published online November 4, 2012

DOI: https://doi.org/10.1007/s12275-012-2411-z

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