Evaluation of a Fosmid-Clone-Based Microarray for Comparative Analysis of Swine Fecal Metagenomes

Soo-Je Park; Dong-Hwan Kim; Man-Young Jung; So-Jeong Kim; Hongik Kim; Yang-Hoon Kim; Jong-Chan Chae; Sung-Keun Rhee

doi:10.1007/s12275-012-2115-4

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Research Support, Non-U.S. Gov't
Evaluation of a Fosmid-Clone-Based Microarray for Comparative Analysis of Swine Fecal Metagenomes: Soo-Je Park ¹, Dong-Hwan Kim ², Man-Young Jung ¹, So-Jeong Kim ¹, Hongik Kim ², Yang-Hoon Kim ¹, Jong-Chan Chae ³, Sung-Keun Rhee ¹; Journal of Microbiology 2012;50(4):684-688.
DOI: https://doi.org/10.1007/s12275-012-2115-4
Published online: July 21, 2012

¹Department of Microbiology, Chungbuk National University, Cheongju 361-763, Republic of Korea, ²R&D Division, VITABIO, Inc., Dasan-gwan 2nd FL., Korea Institute of Oriental Medicine, Daejeon 305-811, Republic of Korea, ³Division of Biotechnology, Chonbuk National University, Iksan 570-752, Republic of Korea

Corresponding author: Sung-Keun Rhee , Tel: +82-43-261-2300,

Received: 2 March 2012 • Accepted: 24 April 2012

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Abstract

Glass slide arrayed with fosmid clone DNAs generated from swine feces as probes were fabricated and used as a metagenome microarray (MGA). MGA appeared to be specific to their corresponding target genomic fragments. The detection limit was 10 ng of genomic DNA (ca. 106 bacterial cells) in the presence of 1000 ng of background DNA. Linear relationships between the signal intensity and the target DNA (20–100 ng) were observed (r2=0.98). Application of MGA to the comparison of swine fecal metagenomes suggested that the microbial community composition of swine intestine could be dependent on the health state of swine.

Keywords: microarray;fosmid clone;gut;community analysis

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Evaluation of a Fosmid-Clone-Based Microarray for Comparative Analysis of Swine Fecal Metagenomes

J. Microbiol. 2012;50(4):684-688. Published online July 21, 2012

DOI: https://doi.org/10.1007/s12275-012-2115-4

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