NOTE] Biosynthetic Pathway for Poly(3-Hydroxypropionate) in Recombinant Escherichia coli

Qi Wang; Changshui Liu; Mo Xian; Yongguang Zhang; Guang Zhao

doi:10.1007/s12275-012-2234-y

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NOTE] Biosynthetic Pathway for Poly(3-Hydroxypropionate) in Recombinant Escherichia coli: Qi Wang ^1,2, Changshui Liu ^1,2, Mo Xian ¹, Yongguang Zhang ¹, Guang Zhao ¹; Journal of Microbiology 2012;50(4):693-697.
DOI: https://doi.org/10.1007/s12275-012-2234-y
Published online: August 25, 2012

¹Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao, 266101, P. R. China, ²Graduate University of Chinese Academy of Sciences, Beijing, 100049, P. R. China

Corresponding author: Guang Zhao , Tel: +86-532-80662767,

Received: 30 April 2012 • Accepted: 4 July 2012

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Abstract

Poly(3-hydroxypropionate) (P3HP) is a biodegradable and biocompatible thermoplastic. In this study, we engineered a P3HP biosynthetic pathway in recombinant Escherichia coli. The genes for malonyl-CoA reductase (mcr, from Chloroflexus aurantiacus), propionyl-CoA synthetase (prpE, from E. coli), and polyhydroxyalkanoate synthase (phaC1, from Ralstonia eutropha) were cloned and expressed in E. coli. The E. coli genes accABCD encoding acetyl-CoA carboxylase were used to channel the carbon into the P3HP pathway. Using glucose as a sole carbon source, the cell yield and P3HP content were 1.32 g/L and 0.98% (wt/wt [cell dry weight]), respectively. Although the yield is relatively low, our study shows the feasibility of engineering a P3HP biosynthetic pathway using a structurally unrelated carbon source in bacteria.

Keywords: poly(3-hydroxypropionate);malonyl-CoA reductase;propionyl-coenzyme A synthetase;polyhydroxyalkanoate synthase;recombinant E. coli

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NOTE] Biosynthetic Pathway for Poly(3-Hydroxypropionate) in Recombinant Escherichia coli

J. Microbiol. 2012;50(4):693-697. Published online August 25, 2012

DOI: https://doi.org/10.1007/s12275-012-2234-y

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