Evaluation of the Cell Growth of Mycobacteria Using Mycobacterium smegmatis mc2 155 as a Representative Species

Jorge A. Gonzalez-y-Merchand; Ruben Zaragoza-Contreras; Rosalina Guadarrama-Medina; Addy C. Helguera-Repetto; Sandra Rivera-Gutierrez; Jorge F. Cerna-Cortes; Leopoldo Santos-Argumedo; Robert A. Cox

doi:10.1007/s12275-012-1556-0

Articles

Page Path: HOME > J. Microbiol > Volume 50(3); 2012 > Article

Research Support, Non-U.S. Gov't
Evaluation of the Cell Growth of Mycobacteria Using Mycobacterium smegmatis mc2 155 as a Representative Species: Jorge A. Gonzalez-y-Merchand ¹, Ruben Zaragoza-Contreras ¹, Rosalina Guadarrama-Medina ¹, Addy C. Helguera-Repetto ¹, Sandra Rivera-Gutierrez ¹, Jorge F. Cerna-Cortes ¹, Leopoldo Santos-Argumedo ², Robert A. Cox ³; Journal of Microbiology 2012;50(3):419-425.
DOI: https://doi.org/10.1007/s12275-012-1556-0
Published online: June 30, 2012

¹Departamento de Microbiologia, Escuela Nacional de Ciencias Biologicas, IPN, Prolong. Carpio y Plan de Ayala s/n, Col. Casco de Santo Tomas, Mexico, D.F. 11340, Mexico, ²Departamento de Biomedicina Molecular, CINVESTAV, Zacatenco, Mexico, D.F., Mexico, ³Division of Mycobacterial Research, National Institute for Medical Research, The Ridgeway, Mill Hill, London NW7 1AA, UK¹Departamento de Microbiologia, Escuela Nacional de Ciencias Biologicas, IPN, Prolong. Carpio y Plan de Ayala s/n, Col. Casco de Santo Tomas, Mexico, D.F. 11340, Mexico, ²Departamento de Biomedicina Molecular, CINVESTAV, Zacatenco, Mexico, D.F., Mexico, ³Division of Mycobacterial Research, National Institute for Medical Research, The Ridgeway, Mill Hill, London NW7 1AA, UK

Corresponding author: Jorge A. Gonzalez-y-Merchand , Tel: +00-5255-57296300,

Received: 7 November 2011 • Accepted: 5 March 2012

prev next

55 Views
0 Download
6 Crossref
8 Scopus

Full Article

Abstract

The study of the in vitro cell growth of mycobacteria still remains a fastidious, difficult, and time-consuming procedure. In addition, assessing mycobacterial growth in the laboratory is often complicated by cell aggregation and slow growth-rate. We now report that the use of a stainless steel spring in the culture led to an absence of large cell clumps, to a decrease of dead cells in the exponential phase and to growth of a more homogeneous population of large cells. We also report that flow cytometry is a rapid, simple and reliable approach to monitor mycobacterial cell growth and viability. Here, we monitored Mycobacterium smegmatis cellular growth by optical density, dry cell mass, and colony forming units; in addition, viability, cell size and granularity profiles were analyzed by flow cytometry, and cell morphology by electron microscopy. Cultures monitored by flow cytometry may lead to a better understanding of the physiology of mycobacteria. Moreover, this methodology may aid in characterizing the cell growth of other fastidious species of microorganisms.

Keywords: mycobacterial growth;mycobacterial physiology;Mycobacterium smegmatis;flow cytometry

Figure & Data

References

Citations

Citations to this article as recorded by

Novel Populations of Mycobacterium smegmatis Under Hypoxia and Starvation: Some Insights on Cell Viability and Morphological Changes
Ruben Zaragoza-Contreras, Diana A. Aguilar-Ayala, Lázaro García-Morales, Miguel A. Ares, Addy Cecilia Helguera-Repetto, Jorge Francisco Cerna-Cortés, Lizbel León-Solis, Fernando Suárez-Sánchez, Jorge A. González-Y-Merchand, Sandra Rivera-Gutiérrez
Microorganisms.2024; 12(11): 2280. CrossRef
Screening of Hydrophilic Polymers Reveals Broad Activity in Protecting Phages during Cryopreservation
Huba L. Marton, Apoorva Bhatt, Antonia P. Sagona, Peter Kilbride, Matthew I. Gibson
Biomacromolecules.2024; 25(1): 413. CrossRef
Flow cytometry method for absolute counting and single-cell phenotyping of mycobacteria
David A. Barr, Charles Omollo, Mandy Mason, Anastasia Koch, Robert J. Wilkinson, David G. Lalloo, Graeme Meintjes, Valerie Mizrahi, Digby F. Warner, Gerry Davies
Scientific Reports.2021;[Epub] CrossRef
A Low Cost/Low Power Open Source Sensor System for Automated Tuberculosis Drug Susceptibility Testing
Kyukwang Kim, Hyeong Kim, Hwijoon Lim, Hyun Myung
Sensors.2016; 16(6): 942. CrossRef
Size evolution in microorganisms masks trade-offs predicted by the growth rate hypothesis
Isabelle Gounand, Tanguy Daufresne, Dominique Gravel, Corinne Bouvier, Thierry Bouvier, Marine Combe, Claire Gougat-Barbera, Franck Poly, Clara Torres-Barceló, Nicolas Mouquet
Proceedings of the Royal Society B: Biological Sciences.2016; 283(1845): 20162272. CrossRef
Global Adaptation to a Lipid Environment Triggers the Dormancy-Related Phenotype of Mycobacterium tuberculosis
Juan G. Rodríguez, Adriana C. Hernández, Cecilia Helguera-Repetto, Diana Aguilar Ayala, Rosalina Guadarrama-Medina, Juan M. Anzóla, Jose R. Bustos, María M. Zambrano, Jorge González-y-Merchand, María J. García, Patricia Del Portillo, Carol A. Nacy
mBio.2014;[Epub] CrossRef

Cite this Article

Cite this Article: export Copy Download Format; Close

Download Citation

Download a citation file in RIS format that can be imported by all major citation management software, including EndNote, ProCite, RefWorks, and Reference Manager.

Format:

RIS — For EndNote, ProCite, RefWorks, and most other reference management software
BibTeX — For JabRef, BibDesk, and other BibTeX-specific software

Include:

Citation for the content below
Citation and abstract for the content below

Evaluation of the Cell Growth of Mycobacteria Using Mycobacterium smegmatis mc2 155 as a Representative Species

J. Microbiol. 2012;50(3):419-425. Published online June 30, 2012

DOI: https://doi.org/10.1007/s12275-012-1556-0

Related articles