Effect of Salmonella Treatment on an Implanted Tumor (CT26) in a Mouse Model

Misun Yun; SangO Pan; Sheng- Nan Jiang; Vu Hong Nguyen; Seung-Hwan Park; Che-Hun Jung; Hyung-Seok Kim; Jung-Joon Min; Hyon E. Choy; Yeongjin Hong

doi:10.1007/s12275-012-2090-9

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Research Support, Non-U.S. Gov't
Effect of Salmonella Treatment on an Implanted Tumor (CT26) in a Mouse Model: Misun Yun ¹, SangO Pan ², Sheng- Nan Jiang ³, Vu Hong Nguyen ³, Seung-Hwan Park ³, Che-Hun Jung ⁴, Hyung-Seok Kim ⁵, Jung-Joon Min ³, Hyon E. Choy ¹, Yeongjin Hong ¹; Journal of Microbiology 2012;50(3):502-510.
DOI: https://doi.org/10.1007/s12275-012-2090-9
Published online: June 30, 2012

¹Departments of Microbiology, Chonnam National University Medical School, Gwangju 501-746, Republic of Korea, ²Jeollanamdo Institute of Natural Resources Research (JINR), Jeollanamdo 529-851, Republic of Korea, ³Departments of Nuclear Medicine, Chonnam National University Medical School, Gwangju 501-746, Republic of Korea, ⁴Departments of Chemistry, Chonnam National University, Gwangju 500-757, Republic of Korea, ⁵Forensic Medicine, Chonnam National University Medical School, Gwangju 501-746, Republic of Korea

Corresponding author: Yeongjin Hong , Tel: +82-61-379-8478,

Received: 16 February 2012 • Accepted: 14 March 2012

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Abstract

The use of bacteria has contributed to recent advances in targeted cancer therapy especially for its tumor-specific accumulation and proliferation. In this study, we investigated the molecular events following bacterial therapy using an attenuated Salmonella Typhimurium defective in ppGpp synthesis (ΔppGpp), by analyzing those proteins differentially expressed in tumor tissues from treated and untreated mice. CT26 murine colon cancer cells were implanted in BALB/c mice and allowed to form tumors. The tumor-bearing mice were treated with the attenuated Salmonella Typhimurium. Tumor tissues were analyzed by 2D-PAGE. Fourteen differentially expressed proteins were identified by mass spectrometry. The analysis revealed that cytoskeletal components, including vimentin, drebrin-like protein, and tropomyosinalpha 3, were decreased while serum proteins related to heme or iron metabolism, including transferrin, hemopexin, and haptoglobin were increased. Subsequent studies revealed that the decrease in cytoskeletal components occurred at the transcriptional level and that the increase in heme and iron metabolism proteins occurred in liver. Most interestingly, the same pattern of increased expression of transferrin, hemopexin, and haptoglobin was observed following radiotherapy at the dosage of 14 Gy.

Keywords: Salmonella Typhimurium;bacterial tumor targeting;CT26;protein response

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Effect of Salmonella Treatment on an Implanted Tumor (CT26) in a Mouse Model

J. Microbiol. 2012;50(3):502-510. Published online June 30, 2012

DOI: https://doi.org/10.1007/s12275-012-2090-9

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