Effects of Exopolysaccharide Production on Liquid Vegetative Growth, Stress Survival, and Stationary Phase Recovery in Myxococcus xanthus

Wei Hu; Jing Wang; Ian McHardy; Renate Lux; Zhe Yang; Yuezhong Li; Wenyuan Shi

doi:10.1007/s12275-012-1349-5

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Research Support, Non-U.S. Gov't
Effects of Exopolysaccharide Production on Liquid Vegetative Growth, Stress Survival, and Stationary Phase Recovery in Myxococcus xanthus: Wei Hu ^1,2, Jing Wang ^1,2, Ian McHardy ³, Renate Lux ¹, Zhe Yang ³, Yuezhong Li ², Wenyuan Shi ^1,3; Journal of Microbiology 2012;50(2):241-248.
DOI: https://doi.org/10.1007/s12275-012-1349-5
Published online: April 27, 2012

¹School of Dentistry and University of California, Los Angeles, CA, 90095, USA, ²State Key Laboratory of Microbial Technology, School of Life Science, Shandong University, Jinan, Shandong, 250100, P. R. China, ³Department of Microbiology, Immunology and Molecular Genetics, University of California, Los Angeles, CA, 90095, USA

Corresponding author: Wenyuan Shi , Tel: +1-310-825-8356,

Received: 19 July 2011 • Accepted: 27 December 2011

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Abstract

Exopolysaccharide (EPS) of Myxococcus xanthus is a wellregulated cell surface component. In addition to its known functions for social motility and fruiting body formation on solid surfaces, EPS has also been proposed to play a role in multi-cellular clumping in liquid medium, though this phenomenon has not been well studied. In this report, we confirmed that M. xanthus clumps formed in liquid were correlated with EPS levels and demonstrated that the EPS encased cell clumps exhibited biofilm-like structures. The clumps protected the cells at physiologically relevant EPS concentrations, while cells lacking EPS exhibited significant reduction in long-term viability and resistance to stressful conditions. However, excess EPS production was counterproductive to vegetative growth and viable cell recovery declined in extended late stationary phase as cells became trapped in the matrix of clumps. Therefore, optimal EPS production by M. xanthus is important for normal physiological functions in liquid.

Keywords: Myxococcus xanthus;exopolysaccharide;vegetative growth;stress survival;stationary phase recovery

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Effects of Exopolysaccharide Production on Liquid Vegetative Growth, Stress Survival, and Stationary Phase Recovery in Myxococcus xanthus

J. Microbiol. 2012;50(2):241-248. Published online April 27, 2012

DOI: https://doi.org/10.1007/s12275-012-1349-5

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