Cyclic AMP-Receptor Protein Activates Aerobactin Receptor IutA Expression in Vibrio vulnificus

Choon-Mee Kim; Seong-Jung Kim; Sung-Heui Shin

doi:10.1007/s12275-012-2056-y

Articles

Page Path: HOME > J. Microbiol > Volume 50(2); 2012 > Article

Research Support, Non-U.S. Gov't
Cyclic AMP-Receptor Protein Activates Aerobactin Receptor IutA Expression in Vibrio vulnificus: Choon-Mee Kim ¹, Seong-Jung Kim ², Sung-Heui Shin ^1,3; Journal of Microbiology 2012;50(2):320-325.
DOI: https://doi.org/10.1007/s12275-012-2056-y
Published online: April 27, 2012

¹Research Center for Resistant Cells, Chosun University Medical School, Gwangju 501-759, Republic of Korea, ²Department of Emergency Medicine, Chosun University Medical School, Gwangju 501-759, Republic of Korea, ³Department of Microbiology, Chosun University Medical School, Gwangju 501-759, Republic of Korea

Corresponding author: Sung-Heui Shin , Tel: +82-62-230-6352,

Received: 1 February 2012 • Accepted: 10 April 2012

prev next

205 Views
0 Download
14 Crossref
12 Scopus

Full Article

Download PDF

Abstract

The ferrophilic bacterium Vibrio vulnificus can utilize the siderophore aerobactin of Escherichia coli for iron acquisition via its specific receptor IutA. This siderophore piracy by V. vulnificus may contribute to its survival and proliferation, especially in mixed bacterial environments. In this study, we examined the effects of glucose, cyclic AMP (cAMP), and cAMP-receptor protein (Crp) on iutA expression in V. vulnificus. Glucose dose-dependently repressed iutA expression. A mutation in cya encoding adenylate cyclase required for cAMP synthesis severely repressed iutA expression, and this change was recovered by in trans complementing cya or the addition of exogenous cAMP. Furthermore, a mutation in crp encoding Crp severely repressed iutA expression, and this change was recovered by complementing crp. Accordingly, glucose deprivation under iron-limited conditions is an environmental signal for iutA expression, and Crp functions as an activator that regulates iutA expression in response to glucose availability.

Keywords: Vibrio vulnificus;iron;aerobactin;glucose;cyclic AMP-receptor protein;siderophore piracy

Figure & Data

References

Citations

Citations to this article as recorded by

Broad‐Spectrum Antimicrobial and Antibiofilm Activities of Halogenated Anilines Against Uropathogenic Escherichia coli and ESKAPE Pathogens
Bharath Reddy Boya, Jin‐Hyung Lee, Jintae Lee
Microbial Biotechnology.2025;[Epub] CrossRef
Aerobactin: A Multifaceted Siderophore
Subho Ghosh
Indian Journal of Microbiology.2025;[Epub] CrossRef
Interspecific cross-talk: The catalyst driving microbial biosynthesis of secondary metabolites
Guihong Yu, Xiaoxuan Ge, Wanting Li, Linwei Ji, Song Yang
Biotechnology Advances.2024; 76: 108420. CrossRef
Impact of substrate on opto-thermal response of thin metallic targets under irradiation with femtosecond laser pulses
G. D. Tsibidis, E. Stratakis
Journal of Central South University.2022; 29(10): 3410. CrossRef
Regulation of iron-uptake systems in Vibrio vulnificus, a ferrophilic bacterium
Sung-Heui Shin
Medical Biological Science and Engineering.2021; 4(2): 69. CrossRef
Characteristics of the iron uptake-related process of a pathogenic Vibrio splendidus strain associated with massive mortalities of the sea cucumber Apostichopus japonicus
Tongxiang Song, Huijie Liu, Tengteng Lv, Xuelin Zhao, Yina Shao, Qingxi Han, Chenghua Li, Weiwei Zhang
Journal of Invertebrate Pathology.2018; 155: 25. CrossRef
Stress responses of Acinetobacter strain Y during phenol degradation
Johnson Lin
Archives of Microbiology.2017; 199(2): 365. CrossRef
Vibrio Iron Transport: Evolutionary Adaptation to Life in Multiple Environments
Shelley M. Payne, Alexandra R. Mey, Elizabeth E. Wyckoff
Microbiology and Molecular Biology Reviews.2016; 80(1): 69. CrossRef
Temperature Change Induces the Expression of vuuA Encoding Vulnibactin Receptor and crp Encoding Cyclic AMP Receptor Protein in Vibrio vulnificus
Choon-Mee Kim, Young-Joon Ahn, Seong-Jung Kim, Dae-Heung Yoon, Sung-Heui Shin
Current Microbiology.2016; 73(1): 54. CrossRef
Cyclic AMP and Cyclic AMP-Receptor Protein are Required for Optimal Capsular Polysaccharide Expression
Hyeon-Kyu Cho, Sung-Heui Shin
Journal of Bacteriology and Virology.2015; 45(1): 26. CrossRef
Enterococcus faecalis reconfigures its transcriptional regulatory network activation at different copper levels
Mauricio Latorre, Jessica Galloway-Peña, Jung Hyeob Roh, Marko Budinich, Angélica Reyes-Jara, Barbara E. Murray, Alejandro Maass, Mauricio González
Metallomics.2014; 6(3): 572. CrossRef
Multiple High-affinity Iron-uptake Systems ofVibrio vulnificus
Sung-Heui Shin
Journal of Bacteriology and Virology.2013; 43(3): 168. CrossRef
Cyclic AMP and Cyclic AMP-receptor Protein Modulate the Autoinducer-2-Mediated Quorum Sensing System in Vibrio vulnificus
Sun-Pyo Kim, Choon-Mee Kim, Sung-Heui Shin
Current Microbiology.2012; 65(6): 701. CrossRef
Coordinate Regulation ofVibrio vulnificusHeme Receptor HupA Expression by Cyclic AMP-receptor Protein and Ferric Uptake Regulator
Sun-Pyo Kim, Gang-Wook Lee, Choon-Mee Kim, Sung-Heui Shin
Journal of Bacteriology and Virology.2012; 42(4): 294. CrossRef

Cite this Article

Cite this Article: export Copy Download Format; Close

Download Citation

Download a citation file in RIS format that can be imported by all major citation management software, including EndNote, ProCite, RefWorks, and Reference Manager.

Format:

RIS — For EndNote, ProCite, RefWorks, and most other reference management software
BibTeX — For JabRef, BibDesk, and other BibTeX-specific software

Include:

Citation for the content below
Citation and abstract for the content below

Cyclic AMP-Receptor Protein Activates Aerobactin Receptor IutA Expression in Vibrio vulnificus

J. Microbiol. 2012;50(2):320-325. Published online April 27, 2012

DOI: https://doi.org/10.1007/s12275-012-2056-y

Related articles