Cyclic AMP-Receptor Protein Activates Aerobactin Receptor IutA Expression in Vibrio vulnificus

Choon-Mee Kim; Seong-Jung Kim; Sung-Heui Shin

doi:10.1007/s12275-012-2056-y

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Cyclic AMP-Receptor Protein Activates Aerobactin Receptor IutA Expression in Vibrio vulnificus: Choon-Mee Kim ¹, Seong-Jung Kim ², Sung-Heui Shin ^1,3; Journal of Microbiology 2012;50(2):320-325.
DOI: https://doi.org/10.1007/s12275-012-2056-y
Published online: April 27, 2012

¹Research Center for Resistant Cells, Chosun University Medical School, Gwangju 501-759, Republic of Korea, ²Department of Emergency Medicine, Chosun University Medical School, Gwangju 501-759, Republic of Korea, ³Department of Microbiology, Chosun University Medical School, Gwangju 501-759, Republic of Korea

Corresponding author: Sung-Heui Shin , Tel: +82-62-230-6352,

Received: 1 February 2012 • Accepted: 10 April 2012

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Abstract

The ferrophilic bacterium Vibrio vulnificus can utilize the siderophore aerobactin of Escherichia coli for iron acquisition via its specific receptor IutA. This siderophore piracy by V. vulnificus may contribute to its survival and proliferation, especially in mixed bacterial environments. In this study, we examined the effects of glucose, cyclic AMP (cAMP), and cAMP-receptor protein (Crp) on iutA expression in V. vulnificus. Glucose dose-dependently repressed iutA expression. A mutation in cya encoding adenylate cyclase required for cAMP synthesis severely repressed iutA expression, and this change was recovered by in trans complementing cya or the addition of exogenous cAMP. Furthermore, a mutation in crp encoding Crp severely repressed iutA expression, and this change was recovered by complementing crp. Accordingly, glucose deprivation under iron-limited conditions is an environmental signal for iutA expression, and Crp functions as an activator that regulates iutA expression in response to glucose availability.

Keywords: Vibrio vulnificus;iron;aerobactin;glucose;cyclic AMP-receptor protein;siderophore piracy

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Cyclic AMP-Receptor Protein Activates Aerobactin Receptor IutA Expression in Vibrio vulnificus

J. Microbiol. 2012;50(2):320-325. Published online April 27, 2012

DOI: https://doi.org/10.1007/s12275-012-2056-y

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