NOTE] Detection of a Unique Fibrinolytic Enzyme in Aeromonas sp. JH1

Han-Young Cho; Min Jeong Seo; Jeong Uck Park; Byoung Won Kang; Gi-Young Kim; Woo Hong Joo; Young-Choon Lee; Yong Kee Jeong

doi:10.1007/s12275-011-1376-7

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NOTE] Detection of a Unique Fibrinolytic Enzyme in Aeromonas sp. JH1: Han-Young Cho ¹, Min Jeong Seo ^2,3,4, Jeong Uck Park ⁴, Byoung Won Kang ⁴, Gi-Young Kim ⁵, Woo Hong Joo ⁶, Young-Choon Lee ^2,3,4, Yong Kee Jeong ^2,3,4; Journal of Microbiology 2011;49(6):1018-1021.
DOI: https://doi.org/10.1007/s12275-011-1376-7
Published online: December 28, 2011

¹Industrial Bio-materials Research Center, Korea Research Institute of Bioscience and Biotechnology, Yusung, Daejeon 305-600, Republic of Korea, ²Department of Biotechnology, Dong-A University, Busan 604-714, Republic of Korea, ³Department of Medical Bioscience, Dong-A University, Busan 604-714, Republic of Korea, ⁴Medi-Farm Industrialization Research Center, Dong-A University, Busan 604-714, Republic of Korea, ⁵Laboratory of Immunobiology, Department of Marine Life Sciences, Jeju National University, Jeju 690-756, Republic of Korea, ⁶Department of Biology, Changwon National University, Kyungnam 641-713, Republic of Korea

Corresponding author: Yong Kee Jeong , Tel: +82-51-200-7557,

Received: 1 August 2011 • Accepted: 2 November 2011

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Abstract

A fibrinolytic enzyme was found in a Gram-negative bacterium, Aeromonas sp. JH1. SDS-PAGE and fibrinzymography showed that it was a 36 kDa, monomeric protein. Of note, the enzyme was highly specific for fibrinogen molecules and the hydrolysis rate of fibrinogen subunits was highest for α, β, and γ chains in that order. The first 15 amino acids of N-terminal sequence were X-D-A-T-G-P-G-G-N-V-X-T-G-K-Y, which was distinguishable from other fibrinolytic enzymes. The optimum pH and temperature of the enzyme were approximately 8.0 and 40°C, respectively. Therefore, these results provide a fibrinolytic enzyme with potent thrombolytic activity from the Aeromonas genus.

Keywords: Amidolytic activity;Aeromonas sp. JH1;fibrinogen subunits;fibrinolytic enzyme;N-terminal amino acid sequence

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NOTE] Detection of a Unique Fibrinolytic Enzyme in Aeromonas sp. JH1

J. Microbiol. 2011;49(6):1018-1021. Published online December 28, 2011

DOI: https://doi.org/10.1007/s12275-011-1376-7

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