A Novel Ribonuclease with Potent HIV-1 Reverse Transcriptase Inhibitory Activity from Cultured Mushroom Schizophyllum commune

Yong-Chang Zhao; Guo-Qing Zhang; Tzi-Bun Ng; He-Xiang Wang

doi:10.1007/s12275-011-1098-x

Articles

Page Path: HOME > J. Microbiol > Volume 49(5); 2011 > Article

Journal Article
A Novel Ribonuclease with Potent HIV-1 Reverse Transcriptase Inhibitory Activity from Cultured Mushroom Schizophyllum commune: Yong-Chang Zhao ¹, Guo-Qing Zhang ^2,3, Tzi-Bun Ng ⁴, He-Xiang Wang ³; Journal of Microbiology 2011;49(5):803-808.
DOI: https://doi.org/10.1007/s12275-011-1098-x
Published online: November 9, 2011

¹Institute of Biotechnology and Germplasmic Resource, Yunnan Academy of Agricultural Science, Kunming 650223, P. R. China, ²Key Laboratory of Urban Agriculture (North) of Ministry of Agriculture, Beijing University of Agriculture, Beijing 102206, P. R. China, ³State Key Laboratory for Agrobiotechnology and Department of Microbiology, China Agricultural University, Beijing 100193, P. R. China, ⁴Department of Biochemistry, Faculty of Medicine, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong, P. R. China¹Institute of Biotechnology and Germplasmic Resource, Yunnan Academy of Agricultural Science, Kunming 650223, P. R. China, ²Key Laboratory of Urban Agriculture (North) of Ministry of Agriculture, Beijing University of Agriculture, Beijing 102206, P. R. China, ³State Key Laboratory for Agrobiotechnology and Department of Microbiology, China Agricultural University, Beijing 100193, P. R. China, ⁴Department of Biochemistry, Faculty of Medicine, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong, P. R. China

Corresponding author: He-Xiang Wang , Tel: +86-10-627-32578,

Received: 2 March 2011 • Accepted: 25 April 2011

prev next

53 Views
0 Download
12 Crossref
15 Scopus

Full Article

Abstract

A 20-kDa ribonuclease (RNase) was purified from fresh fruiting bodies of cultured Schizophyllum commune mushrooms. The RNase was not adsorbed on Affi-gel blue gel but adsorbed on DEAE-cellulose and CM-cellulose. It exhibited maximal RNase activity at pH 6.0 and 70°C. It demonstrated the highest ribonucleolytic activity toward poly (U) (379.5 μ/mg), the second highest activity toward poly (C) (244.7 μ/mg), less activity toward poly (A) (167.4 μ/mg), and much weaker activity toward poly (G) (114.5 μ/mg). The RNase inhibited HIV-1 reverse transcriptase with an IC50 of 65 μM. No effect on [3H-methyl]-thymidine uptake by lymphoma MBL2 cells and leukemia L1210 cells was observed at 100 μM concentration of the RNase. A comparison of RNases from S. commune and Volvariella volvacea revealed that they demonstrated some similarities in N-terminal amino acid sequence, optimum pH and polyhomoribonucleotide specificity. However, some differences in chromatographic behavior and molecular mass were observed.

Keywords: Schizophyllum commune;mushroom;purification;ribonuclease

Figure & Data

References

Citations

Citations to this article as recorded by

Schizophyllum commune, an underrated edible and medicinal mushroom: farm to industry
Disha Dasgupta, Pranjali Basu, Anamika Paul, Krishnendu Acharya, Nilanjan Chakraborty
Studies in Fungi.2025;[Epub] CrossRef
Investigation of the inhibition of respiratory bacterial pathogens and HIV-1 enzymes by twenty-one South African mushroom species
Jenske Didloff, Gerhardt J. Boukes, Maryna van de Venter, Bennie Viljoen, Michael Lee, Candice Blom, Rebecca A. Dwyer, Sharlene Govender
South African Journal of Botany.2024; 166: 375. CrossRef
The Health-promoting Potential of Edible Mushroom Proteins
Ana Sofia Sousa, Helena Araújo-Rodrigues, Manuela Estevez Pintado
Current Pharmaceutical Design.2023; 29(11): 804. CrossRef
Evidence for the Presence of Hyphae and Fruiting Body Calcium Oxalate Crystallites in the Split Gill Medicinal Mushroom Schizophyllum commune (Agaricomycetes)
Xiangyue Xiao, Tianji Huang, Jingyi Zhang, Wei Liu, Hong Ji, Nemat O. Keyhani, Hui Chen, Weijie Wu, Chi Song
International Journal of Medicinal Mushrooms.2022; 24(10): 83. CrossRef
Medicinal Fungi with Antiviral Effect
Yu Zhang, Guoying Zhang, Jianya Ling
Molecules.2022; 27(14): 4457. CrossRef
Narrative Review: Bioactive Potential of Various Mushrooms as the Treasure of Versatile Therapeutic Natural Product
Hitesh Chopra, Awdhesh Kumar Mishra, Atif Amin Baig, Tapan Kumar Mohanta, Yugal Kishore Mohanta, Kwang-Hyun Baek
Journal of Fungi.2021; 7(9): 728. CrossRef
Lectins purified from medicinal and edible mushrooms: Insights into their antiviral activity against pathogenic viruses
Yousra A. El-Maradny, Esmail M. El-Fakharany, Marwa M. Abu-Serie, Mona H. Hashish, Heba S. Selim
International Journal of Biological Macromolecules.2021; 179: 239. CrossRef
Research Progress of Bioactive Proteins from the Edible and Medicinal Mushrooms
Rong Zhou, Zhao Kun Liu, Ye Ni Zhang, Jack Ho Wong, Tzi Bun Ng, Fang Liu
Current Protein & Peptide Science.2019; 20(3): 196. CrossRef
A novel ribonuclease with antiproliferative activity toward leukemia and lymphoma cells and HIV-1 reverse transcriptase inhibitory activity from the mushroom, Hohenbuehelia serotina
RUI ZHANG, LIYAN ZHAO, HEXIANG WANG, TZI BUN NG
International Journal of Molecular Medicine.2014; 33(1): 209. CrossRef
A Novel Ribonuclease with HIV-1 Reverse Transcriptase Inhibitory Activity from the Edible Mushroom Hygrophorus russula
Mengjuan Zhu, Lijing Xu, Xiao Chen, Zengqiang Ma, Hexiang Wang, T. B. Ng
Applied Biochemistry and Biotechnology.2013; 170(1): 219. CrossRef
Purification and characterization of a novel acid phosphatase from the split gill mushroom Schizophyllum commune
Guo‐Qing Zhang, Qing‐Jun Chen, Jian Sun, He‐Xiang Wang, Chun‐Hua Han
Journal of Basic Microbiology.2013; 53(10): 868. CrossRef
Purification and characterization of a protein with antifungal, antiproliferative, and HIV‐1 reverse transcriptase inhibitory activities from small brown‐eyed cowpea seeds
Guo‐Ting Tian, Meng Juan Zhu, Ying Ying Wu, Qin Liu, He Xiang Wang, Tzi Bun Ng
Biotechnology and Applied Biochemistry.2013; 60(4): 393. CrossRef

Cite this Article

Cite this Article: export Copy Download Format; Close

Download Citation

Download a citation file in RIS format that can be imported by all major citation management software, including EndNote, ProCite, RefWorks, and Reference Manager.

Format:

RIS — For EndNote, ProCite, RefWorks, and most other reference management software
BibTeX — For JabRef, BibDesk, and other BibTeX-specific software

Include:

Citation for the content below
Citation and abstract for the content below

A Novel Ribonuclease with Potent HIV-1 Reverse Transcriptase Inhibitory Activity from Cultured Mushroom Schizophyllum commune

J. Microbiol. 2011;49(5):803-808. Published online November 9, 2011

DOI: https://doi.org/10.1007/s12275-011-1098-x

Related articles