Berberine Inhibits HEp-2 Cell Invasion Induced by Chlamydophila pneumoniae Infection

Li Jun Zhang; Li Jun Zhang; Wei Quan; Bei Bei Wang; Bing Ling Shen; Teng Teng Zhang; Yi Kang

doi:10.1007/s12275-011-1051-z

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Research Support, Non-U.S. Gov't
Berberine Inhibits HEp-2 Cell Invasion Induced by Chlamydophila pneumoniae Infection: Li Jun Zhang ¹, Li Jun Zhang ¹, Wei Quan ¹, Bei Bei Wang ¹, Bing Ling Shen ¹, Teng Teng Zhang ¹, Yi Kang ²; Journal of Microbiology 2011;49(5):834-840.
DOI: https://doi.org/10.1007/s12275-011-1051-z
Published online: November 9, 2011

¹Department of Pathophysiology, ²Department of Pharmacology, Basic Medical School of Tianjin Medical University, Tianjin 300070, P. R. China

Corresponding author: Li Jun Zhang , Tel: +86-22-2354-2745,

Received: 31 January 2011 • Accepted: 18 May 2011

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Abstract

This study investigated the inhibitory effects of berberine on Chlamydophila (Chlamydia) pneumoniae infection-induced HEp-2 cell invasion and explored the possible mechanisms involved in this process. C. pneumoniae infection resulted in a significant increase in HEp-2 cell invasion when compared with the control cells (P<0.01) in a Matrigel invasion assay. This enhanced cell invasion was strongly suppressed by berberine (50 μM) (P<0.01). In a cell adhesion assay, the infection-induced HEp-2 cell adhesion to Matrigel was also significantly inhibited by berberine (P<0.01). C. pneumoniae infection was found to promote HEp-2 cell migration remarkably (P<0.01), which was markedly suppressed by berberine (P<0.01) in the cell migration assays. There were no statistically significant differences in the expression of matrix metalloproteinase-1 (MMP-1) and MMP-9 in the infected cells and berberine did not change the expression of MMP-1 and MMP-9. These data suggest that berberine inhibits C. pneumoniae infection-induced HEp-2 cell invasion through suppressing HEp-2 cell adhesion and migration, but not through changing the expression of MMP-1 and MMP-9.

Keywords: berberine;Chlamydia pneumoniae;cell invasion;cell adhesion;cell migration

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Berberine Inhibits HEp-2 Cell Invasion Induced by Chlamydophila pneumoniae Infection

J. Microbiol. 2011;49(5):834-840. Published online November 9, 2011

DOI: https://doi.org/10.1007/s12275-011-1051-z

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