Methyl Coenzyme M Reductase (mcrA) Gene Based Phylogenetic Analysis of Methanogens Population in Murrah Buffaloes (Bubalus bubalis)

Prem Prashant Chaudhary; Sunil Kumar Sirohi; Dheer Singh; Jyoti Saxena

doi:10.1007/s12275-011-1052-y

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Methyl Coenzyme M Reductase (mcrA) Gene Based Phylogenetic Analysis of Methanogens Population in Murrah Buffaloes (Bubalus bubalis): Prem Prashant Chaudhary ^1,2, Sunil Kumar Sirohi ¹, Dheer Singh ³, Jyoti Saxena ²; Journal of Microbiology 2011;49(4):558-561.
DOI: https://doi.org/10.1007/s12275-011-1052-y
Published online: September 2, 2011

¹Nutrition Biotechnology Lab, Animal Biotechnology Centre, National Dairy Research Institute, Karnal-132001, Haryana, India, ²Department of Biosciences and Biotechnology, Banasthali University, Rajasthan-304022, India, ³Animal Biochemistry Division, National Dairy Research Institute, Karnal-132001, Haryana, India

Corresponding author: Sunil Kumar Sirohi , Tel: +92-0184-2251536,

Received: 31 January 2011 • Accepted: 16 March 2011

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Abstract

The aim of the present study was to decipher the diversity of methanogens in rumen of Murrah buffaloes so that effective strategies can be made in order to mitigate methane emission from these methanogens. In the present study diversity of rumen methanogens in Murrah buffaloes (Bubalus bubalis) from North India was evaluated by using mcr-A gene library obtained from the pooled PCR product from four animals and by using MEGA4 software. A total of 104 clones were examined, revealing 26 different mcr-A gene sequences or phylotypes. Of the 26 phylotypes, 16 (64 of 104 clones) were less than 97% similar to any of the cultured strain of methanogens. Seven clone sequences were clustered with Methanomicrobium mobile and three clone sequences were clustered with Methanobrevibacter gottschalkii during the phylogenetic analysis. Uncultured group of methanogens comes out to be the major component of the methanogens community structure in Murrah buffaloes. Methanomicrobium phylotype comes out to be major phylotype among cultured methanogens followed by Methanobrevibacter phylotype. These results help in making effective strategies to check the growth of dominant methanogenic communities in the rumen of this animal which in turn help in the reduction of methane emission in the environment and ultimately helps us in fighting with the problem of global warming.

Keywords: mcr-A gene;methanogens;Murrah buffaloes;phylogenetics

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Methyl Coenzyme M Reductase (mcrA) Gene Based Phylogenetic Analysis of Methanogens Population in Murrah Buffaloes (Bubalus bubalis)

J. Microbiol. 2011;49(4):558-561. Published online September 2, 2011

DOI: https://doi.org/10.1007/s12275-011-1052-y

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