Genotypic and Phenotypic Characteristics of Tunisian Isoniazid-Resistant Mycobacterium tuberculosis Strains

Alya Soudani; Meriem Zribi; Feriel Messaadi; Taieb Messaoud; Afef Masmoudi; Mohamed Zribi; Chedlia Fendri

doi:10.1007/s12275-011-0268-1

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Genotypic and Phenotypic Characteristics of Tunisian Isoniazid-Resistant Mycobacterium tuberculosis Strains: Alya Soudani ^{1 Sondess Hadjf}, Meriem Zribi ¹, Feriel Messaadi ³, Taieb Messaoud ², Afef Masmoudi ¹, Mohamed Zribi ³, Chedlia Fendri ¹; Journal of Microbiology 2011;49(3):413-417.
DOI: https://doi.org/10.1007/s12275-011-0268-1
Published online: June 30, 2011

¹Microbiology Laboratory, Rabta University Hospital, Tunis 1007, Tunisia, ²Biochemistry and Molecular Laboratory, Children Hospital, Tunis 1007, Tunisia, ³Hygiene Laboratory, Hedi Chaker Hospital, Sfax 3000, Tunisia

Corresponding author: Alya Soudani , Tel: +21622602107,

Received: 22 July 2010 • Accepted: 3 January 2011

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Abstract

Forty three isoniazid (INH)-resistant Mycobacterium tuberculosis isolates were characterized on the basis of the most common INH associated mutations, katG315 and mabA -15C→T, and phenotypic properties (i.e. MIC of INH, resistance associated pattern, and catalase activity). Typing for resistance mutations was performed by Multiplex Allele-Specific PCR and sequencing reaction. Mutations at either codon were detected in 67.5% of isolates: katG315 in 37.2, mabA -15C→T in 27.9 and both of them in 2.4%, respectively. katG sequencing showed a G insertion at codon 325 detected in 2 strains and leading to amino acid change T326D which has not been previously reported. Distribution of each mutation, among the investigated strains, showed that katG S315T was associated with multiple-drug profile, high-level INH resistance and loss or decreased catalase activity; whereas the mabA -15C→T was more prevalent in mono-INH resistant isolates, but it was not only associated with a low-level INH resistance. It seems that determination of catalase activity aids in the detection of isolates for which MICs are high and could, in conjunction with molecular methods, provide rapid detection of most clinical INH-resistant strains.

Keywords: M. tuberculosis;isoniazid resistance;new katG326 mutation;katG315 and mabA -15C→T mutations;phenotypic properties

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Genotypic and Phenotypic Characteristics of Tunisian Isoniazid-Resistant Mycobacterium tuberculosis Strains

J. Microbiol. 2011;49(3):413-417. Published online June 30, 2011

DOI: https://doi.org/10.1007/s12275-011-0268-1

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