The MpkB MAP Kinase Plays a Role in Post-karyogamy Processes as well as in Hyphal Anastomosis During Sexual Development in Aspergillus nidulans

Sang-Cheol Jun; Sei-Jin Lee; Hyun-Joo Park; Ji-Young Kang; Young-Eun Leem; Tae-Ho Yang; Mi-Hee Chang; Jung-Mi Kim; Seung-Hwan Jang; Hwan-Gyu Kim; Dong-Min Han; Keon-Sang Chae; Kwang-Yeop Jahng

doi:10.1007/s12275-011-0193-3

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The MpkB MAP Kinase Plays a Role in Post-karyogamy Processes as well as in Hyphal Anastomosis During Sexual Development in Aspergillus nidulans: Sang-Cheol Jun ¹, Sei-Jin Lee ², Hyun-Joo Park ¹, Ji-Young Kang ¹, Young-Eun Leem ¹, Tae-Ho Yang ¹, Mi-Hee Chang ¹, Jung-Mi Kim ¹, Seung-Hwan Jang ¹, Hwan-Gyu Kim ¹, Dong-Min Han ³, Keon-Sang Chae ¹, Kwang-Yeop Jahng ¹; Journal of Microbiology 2011;49(3):418-430.
DOI: https://doi.org/10.1007/s12275-011-0193-3
Published online: June 30, 2011

¹Division of Biological Sciences, Institute for Basic Sciences, Institute of Bioactive Materials, Chonbuk National University, Jeonju 561-756, Republic of Korea, ²Korea Basic Science Institute Jeonju Center, Wonkwang University, Iksan 570-749, Republic of Korea, ³Division of Life Sciences, Wonkwang University, Iksan 570-749, Republic of Korea¹Division of Biological Sciences, Institute for Basic Sciences, Institute of Bioactive Materials, Chonbuk National University, Jeonju 561-756, Republic of Korea, ²Korea Basic Science Institute Jeonju Center, Wonkwang University, Iksan 570-749, Republic of Korea, ³Division of Life Sciences, Wonkwang University, Iksan 570-749, Republic of Korea

Corresponding author: Kwang-Yeop Jahng , Tel: +82-63-270-3358,

Received: 25 May 2010 • Accepted: 10 January 2011

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Abstract

Two genes encoding MAP kinase homologs, designated as mpkB and mpkC, were isolated from Aspergillus nidulans by PCR with degenerate primers. Deletion and over-expression mutants of mpkC showed no detectable phenotypes under any external stress tested. Deletion of mpkB caused pleiotropic phenotypes including a failure in forming cleistothecia under any induction conditions for sexual development, increased Hülle cell production, slow hyphal growth and aberrant conidiophore morphology. Over-expression of mpkB led to increased cleistothecium production. While the transcripts of mpkB and mpkC were constitutively synthesized through the entire life cycle, their size and amount differed with developmental stages. An outcross test using fluorescent protein reporters showed that the mpkB deletion mutant could not form heterokaryons with wild type. Protoplast fusion experiments showed that the fusant of the mpkB mutant with wild type could undergo normal sexual development. However, heterokaryotic mycelia that were produced from a fusant between two mpkB deletion mutants could not form cleistothecia, although they did appear to form diploid nuclei. These results suggest that the MpkB MAP kinase is required for some post-karyogamy process as well as at the hyphal anastomosis stage to accomplish sexual development successfully.

Keywords: Aspergillus nidulans;MpkB MAP kinase;Hü;lle cell;post-karyogamy;anastomosis

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The MpkB MAP Kinase Plays a Role in Post-karyogamy Processes as well as in Hyphal Anastomosis During Sexual Development in Aspergillus nidulans

J. Microbiol. 2011;49(3):418-430. Published online June 30, 2011

DOI: https://doi.org/10.1007/s12275-011-0193-3

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