Identification of PhoB Binding Sites of the yibD and ytfK Promoter Regions in Escherichia coli

Yusuke Yoshida; Shinichiro Sugiyama; Tomoya Oyamada; Katsushi Yokoyama; Soo-Ki Kim; Kozo Makino

doi:10.1007/s12275-011-0360-6

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Identification of PhoB Binding Sites of the yibD and ytfK Promoter Regions in Escherichia coli: Yusuke Yoshida ¹, Shinichiro Sugiyama ¹, Tomoya Oyamada ¹, Katsushi Yokoyama ¹, Soo-Ki Kim ², Kozo Makino ¹; Journal of Microbiology 2011;49(2):285-289.
DOI: https://doi.org/10.1007/s12275-011-0360-6
Published online: May 3, 2011

¹Department of Applied Chemistry, National Defense Academy, Yokosuka 239-8686, Japan, ²Department of Animal Science and Environment, College of Animal Bioscience and Biotechnology, Konkuk University, Seoul 143-701, Republic of Korea

Corresponding author: Kozo Makino , Tel: +81-468-41-3810,

Received: 8 September 2010 • Accepted: 10 November 2010

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Abstract

By using a lacZ operon fusion genomic library of the Escherichia coli O157:H7 Sakai, we identified phosphatestarvation-inducible (psi) promoters located upstream of the yibD and ytfK genes. They have been previously proposed to belong to the phosphate regulon (pho regulon) by Beak and Lee (2006), based on the DNA array and in vivo transcriptional experiments. However, the direct interaction of these promoters with the activator protein of the pho regulon, PhoB, has not been determined. We determined the binding regions of PhoB in these promoter regions by DNase I footprinting. Both regions contained two pho boxes similar to the consensus sequence for PhoB binding.

Keywords: Escherichia coli;pho regulon;two-component regulatory system;yibD;ytfK

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Identification of PhoB Binding Sites of the yibD and ytfK Promoter Regions in Escherichia coli

J. Microbiol. 2011;49(2):285-289. Published online May 3, 2011

DOI: https://doi.org/10.1007/s12275-011-0360-6

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