NOTE] Development of Porphyromonas gingivalis-Specific Quantitative Real-Time PCR Primers Based on the Nucleotide Sequence of rpoB

Soon-Nang Park; Jae-Yoon Park; Joong-Ki Kook

doi:10.1007/s12275-011-1028-y

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Research Support, Non-U.S. Gov't
NOTE] Development of Porphyromonas gingivalis-Specific Quantitative Real-Time PCR Primers Based on the Nucleotide Sequence of rpoB: Soon-Nang Park ^1,2, Jae-Yoon Park ³, Joong-Ki Kook ^1,2; Journal of Microbiology 2011;49(2):315-319.
DOI: https://doi.org/10.1007/s12275-011-1028-y
Published online: May 3, 2011

¹Department of Oral Biochemistry, School of Dentistry, Chosun University, Gwangju 501-759, Republic of Korea, ²Oral Biology Research Institute, School of Dentistry, Chosun University, Gwangju 501-759, Republic of Korea, ³Department of Biochemistry, Chosun University, Gwangju 501-759, Republic of Korea

Corresponding author: Joong-Ki Kook , Tel: +82-62-230-6877,

Received: 18 January 2011 • Accepted: 15 February 2011

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Abstract

Species-specific quantitative real-time PCR (qPCR) primers were developed for the detection of Porphyromonas gingivalis. These primers, Pg-F/Pg-R, were designed based on the nucleotide sequences of RNA polymerase β-subunit gene (rpoB). Species-specific amplicons were obtained from the tested P. gingivalis strains but not in any of the other strains (46 strains of 46 species). The qPCR primers could detect as little as 4 fg of P. gingivalis chromosomal DNA. These findings suggest that these qPCR primers are suitable for applications in epidemiological studies.

Keywords: Porphyromonas gingivalis;rpoB;species-specific;qPCR primers

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NOTE] Development of Porphyromonas gingivalis-Specific Quantitative Real-Time PCR Primers Based on the Nucleotide Sequence of rpoB

J. Microbiol. 2011;49(2):315-319. Published online May 3, 2011

DOI: https://doi.org/10.1007/s12275-011-1028-y

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