Multilocus Sequence Typing and Virulence Factors Analysis of Escherichia coli O157 Strains in China

Xiao W. Ji; Ya L. Liao; Ye F. Zhu; Hai G. Wang; Ling Gu; Jiang Gu; Chen Dong; Hong L. Ding; Xu H. Mao; Feng C. Zhu; Quan M. Zou

doi:10.1007/s12275-010-0132-8

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Multilocus Sequence Typing and Virulence Factors Analysis of Escherichia coli O157 Strains in China: Xiao W. Ji ¹, Ya L. Liao ¹, Ye F. Zhu ², Hai G. Wang ¹, Ling Gu ², Jiang Gu ¹, Chen Dong ², Hong L. Ding ¹, Xu H. Mao ¹, Feng C. Zhu ², Quan M. Zou ¹; Journal of Microbiology 2010;48(6):849-855.
DOI: https://doi.org/10.1007/s12275-010-0132-8
Published online: January 9, 2011

¹Department of Clinical Micbiology and Immunology, Faculty of Medical Laboratory Science, the Third Military Medical University, Chongqing 400038, P. R. China , ²Jiangsu Centres for Disesase Prevention and Control, 210009, P. R. China¹Department of Clinical Micbiology and Immunology, Faculty of Medical Laboratory Science, the Third Military Medical University, Chongqing 400038, P. R. China , ²Jiangsu Centres for Disesase Prevention and Control, 210009, P. R. China

Corresponding author: Feng C. Zhu , Tel: +86-23-6875-2316,
Quan M. Zou , Tel: +86-23-6875-2316,

Received: 8 April 2010 • Accepted: 17 August 2010

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Abstract

Escherichia coli O157:H7, an important food-borne pathogen, has become a major public health concern worldwide. The aim of this study was to investigate the molecular epidemiologic feature of E. coli O157:H7 strains in China. 105 E. coli O157:H7 isolates were collected from various hosts and places over 9 years. A multilocus sequence typing scheme (MLST) was applied for bacteria genotyping and polymerase chain reaction (PCR) was used for virulence factor identification. Seven new MLST sequence types (STs), namely ST836, ST837, ST838, ST839, ST840, ST841, and ST842 were identified, which grouped into two lineages. Phylogenetic analysis suggested that the most two frequent STs in China, ST837 and ST836, may be the derivatives of E. coli O157:H7 Sakai or E. coli O157:H7 EDL933. Geographical diversity and host variety of E. coli O157:H7 were observed in China. In addition, the different distribution of tccp was detected. The data presented herein provide new insights into the molecular epidemiologic feature of E. coli O157:H7, and aid in the investigation of the transmission regularity and evolutionary mechanism of E. coli O157:H7.

Keywords: E. coli O157: H7;tccp;STs;stx

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Multilocus Sequence Typing and Virulence Factors Analysis of Escherichia coli O157 Strains in China

J. Microbiol. 2010;48(6):849-855. Published online January 9, 2011

DOI: https://doi.org/10.1007/s12275-010-0132-8

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