Identification of Vibrio natriegens uvrA and uvrB Genes and Analysis of Gene Regulation Using Transcriptional Reporter Plasmids

Keryn L. Simons; Susan M. Thomas; Peter A. Anderson

doi:10.1007/s12275-010-9370-z

Articles

Page Path: HOME > J. Microbiol > Volume 48(5); 2010 > Article

Research Support, Non-U.S. Gov't
Identification of Vibrio natriegens uvrA and uvrB Genes and Analysis of Gene Regulation Using Transcriptional Reporter Plasmids: Keryn L. Simons ¹, Susan M. Thomas ², Peter A. Anderson ¹; Journal of Microbiology 2010;48(5):644-656.
DOI: https://doi.org/10.1007/s12275-010-9370-z
Published online: November 3, 2010

¹School of Biological Sciences, Flinders University, Adelaide, S.A, 5001, Australia, ²Charles Sturt University, Wagga Wagga, N.S.W, 2678, Australia

Corresponding author: Keryn L. Simons , Tel: +61-8-8201-7961,

Received: 1 December 2009 • Accepted: 16 May 2010

prev next

66 Views
0 Download
6 Crossref
6 Scopus

Full Article

Abstract

Nucleotide excision repair (NER) rectifies a variety of chemically and structurally distinct DNA lesions. The current model of NER is based upon the enteric bacterium Escherichia coli and there is scarce information about how other bacterial species respond to, and correct, DNA damage. Here we report the isolation and functional analysis of the uvrA and uvrB genes from Vibrio natriegens, a naturally occurring marine bacterium. Genetic studies were completed to assess the repair capabilities of V. natriegens uvrA and uvrB in E. coli uvrA and uvrB mutants. In addition to the genetic studies, transcriptional fusions between the luciferase gene and the 5′ regulatory regions of uvrA and uvrB gene of V. natriegens and E. coli were constructed. Luminescent measurements from E. coli transformed with these constructs showed that whilst the response to UV irradiation of either E. coli or V. natriegens uvrA regulatory sequences was similar, both the rate and induction of luminescence detected from the uvrB regulatory regions differed.

Keywords: NER;uvrA;uvrB;V. natriegens;luxAB

Figure & Data

References

Citations

Citations to this article as recorded by

Taking Synthetic Biology to the Seas: From Blue Chassis Organisms to Marine Aquaforming
Lennart Schada von Borzyskowski
ChemBioChem.2023;[Epub] CrossRef
Redesign of ultrasensitive and robust RecA gene circuit to sense DNA damage
Jack X. Chen, Boon Lim, Harrison Steel, Yizhi Song, Mengmeng Ji, Wei E. Huang
Microbial Biotechnology.2021; 14(6): 2481. CrossRef
Vibrio natriegens: an ultrafast‐growing marine bacterium as emerging synthetic biology chassis
Josef Hoff, Benjamin Daniel, Daniel Stukenberg, B W. Thuronyi, Torsten Waldminghaus, Georg Fritz
Environmental Microbiology.2020; 22(10): 4394. CrossRef
Construction of a bioreporter by heterogeneously expressing a Vibrio natriegens recA::luxCDABE fusion in Escherichia coli, and genotoxicity assessments of petrochemical-contaminated groundwater in northern China
Bo Jiang, Wei E. Huang, Guanghe Li
Environmental Science: Processes & Impacts.2016; 18(6): 751. CrossRef
The application of a carrier-based bioremediation strategy for marine oil spills
Petra J. Sheppard, Keryn L. Simons, Eric M. Adetutu, Krishna K. Kadali, Albert L. Juhasz, Mike Manefield, Priyangshu M Sarma, Banwari Lal, Andrew S. Ball
Marine Pollution Bulletin.2014; 84(1-2): 339. CrossRef
Carrier mounted bacterial consortium facilitates oil remediation in the marine environment
Keryn L. Simons, Petra J. Sheppard, Eric M. Adetutu, Krishna Kadali, Albert L. Juhasz, Mike Manefield, Priyangshu M. Sarma, Banwari Lal, Andrew S. Ball
Bioresource Technology.2013; 134: 107. CrossRef

Cite this Article

Cite this Article: export Copy Download Format; Close

Download Citation

Download a citation file in RIS format that can be imported by all major citation management software, including EndNote, ProCite, RefWorks, and Reference Manager.

Format:

RIS — For EndNote, ProCite, RefWorks, and most other reference management software
BibTeX — For JabRef, BibDesk, and other BibTeX-specific software

Include:

Citation for the content below
Citation and abstract for the content below

Identification of Vibrio natriegens uvrA and uvrB Genes and Analysis of Gene Regulation Using Transcriptional Reporter Plasmids

J. Microbiol. 2010;48(5):644-656. Published online November 3, 2010

DOI: https://doi.org/10.1007/s12275-010-9370-z

Related articles