Purification and Characterization of the α-Glucosidase Produced by Thermophilic Fungus Thermoascus aurantiacus CBMAI 756

Ana Flávia Azevedo Carvalho; Maurício Boscolo; Roberto da Silva; Henrique Ferreira; Eleni Gomes

doi:10.1007/s12275-010-9319-2

Articles

Page Path: HOME > J. Microbiol > Volume 48(4); 2010 > Article

Research Support, Non-U.S. Gov't
Purification and Characterization of the α-Glucosidase Produced by Thermophilic Fungus Thermoascus aurantiacus CBMAI 756: Ana Flávia Azevedo Carvalho , Maurício Boscolo , Roberto da Silva , Henrique Ferreira , Eleni Gomes; Journal of Microbiology 2010;48(4):452-459.
DOI: https://doi.org/10.1007/s12275-010-9319-2
Published online: August 20, 2010

Laboratory of Bichemistry and Applied Microbiology, São Paulo State University-Unesp, São José do Rio Preto 15054-000, Brazil

Corresponding author: Ana Flávia Azevedo Carvalho , Tel: +55-17-3221-2393,

Received: 14 October 2009 • Accepted: 23 April 2010

prev next

309 Views
0 Download
8 Crossref
11 Scopus

Full Article

Download PDF

Abstract

Αn α-glucosidase enzyme produced by the fungus Thermoascus aurantiacus CBMAI 756 was purified by ultra filtration, ammonium sulphate precipitation, and chromatography using Q Sepharose, Sephacryl S-200, and Superose 12 columns. The apparent molecular mass of the enzyme was 83 kDa as determined in gel electrophoresis. Maximum activity was observed at pH 4.5 at 70°C. Enzyme showed stability stable in the pH range of 3.0-9.0 and lost 40% of its initial activity at the temperatures of 40, 50, and 60°C. In the presence of ions Na+, Ba2+, Co2+, Ni2+, Mg2+, Mn2+, Al3+, Zn2+, Ca2+ this enzyme maintained 90-105% of its maximum activity and was inhibited by Cr3+, Ag+, and Hg2+. The enzyme showed a transglycosylation property, by the release of oligosaccharides after 3 h of incubation with maltose, and specificity for short maltooligosaccharides and α-PNPG. The Km measured for the α-glucosidase was 0.07 μM, with a Vmax of 318.0 μmol/min/mg.

Keywords: α-glucosidase;T. aurantiacus;termostable enzyme;transglycosylation reaction;purification

Figure & Data

References

Citations

Citations to this article as recorded by

Enhanced Production, Purification, and Characterization of α-Glucosidase from NTG-Mutagenized Aspergillus niger for Industrial Applications
Bowei Yao, Qian Liu, Junjie Xiong, Guangming Feng, Zhongyi Chang, Hongliang Gao
Catalysts.2025; 15(5): 450. CrossRef
Biotechnological Potential of a Glucose-Tolerant β-Glucosidase from Rasamsonia composticola (β-GluRc) in Sugarcane Bagasse Saccharification
Isabela Pavão Vargas, Rodrigo Mattos Silva Galeano, Aline Pereira de Almeida, Ana Lorena de Oliveira Simas, João Victor Souza Ribeiro, Maria de Lourdes Teixeira de Moraes Polizeli, Douglas Chodi Masui, Giovana Cristina Giannesi, Roberto Ruller, Fabiana Fo
Waste and Biomass Valorization.2025;[Epub] CrossRef
Purification, characterization of a novel α-glucosidase from Debaryomyces hansenii strain MCC 0202 and chromatographic separation for high purity isomalto-oligosaccharides production
Saravanan Rengarajan, Rameshthangam Palanivel
Process Biochemistry.2024; 136: 109. CrossRef
Chemical Constituents, Antioxidant, and α-Glucosidase Inhibitory Activities of Different Fermented Gynostemma Pentaphyllum Leaves and Untargeted Metabolomic Measurement of the Metabolite Variation
Xuechun Zhang, Shi Li, Zhibin Zhang, Kin Weng Kong, Zhenxing Wang, Xiahong He
Antioxidants.2023; 12(8): 1505. CrossRef
The development of a heterologous gene expression system in thermophilic fungus Thermoascus aurantiacus
Shenglin Hu, Zhefan Wang, Dongmei Wang, Jichao Wang, Jiong Hong
3 Biotech.2021;[Epub] CrossRef
Production and Catalytic Properties of Amylases fromLichtheimia ramosaandThermoascus aurantiacusby Solid-State Fermentation
Ana Paula Aguero de Oliveira, Maria Alice Silvestre, Nayara Fernanda Lisboa Garcia, Heloíza Ferreira Alves-Prado, André Rodrigues, Marcelo Fossa da Paz, Gustavo Graciano Fonseca, Rodrigo Simões Ribeiro Leite
The Scientific World Journal.2016; 2016: 1. CrossRef
Purification and characterization of a novel α-glucosidase from Malbranchea cinnamomea
Q. J. Yan, P. Han, S. Q. Yang, Z. Q. Jiang
Biotechnology Letters.2015; 37(6): 1279. CrossRef
Biochemical properties of an extracellular trehalase from Malbranchea pulchella var. Sulfurea
Marita Gimenez Pereira, Luis Henrique Souza Guimarães, Rosa Prazeres Melo Furriel, Maria de Lourdes Teixeira de Moraes Polizeli, Hector Francisco Terenzi, João Atílio Jorge
The Journal of Microbiology.2011; 49(5): 809. CrossRef

Cite this Article

Cite this Article: export Copy Download Format; Close

Download Citation

Download a citation file in RIS format that can be imported by all major citation management software, including EndNote, ProCite, RefWorks, and Reference Manager.

Format:

RIS — For EndNote, ProCite, RefWorks, and most other reference management software
BibTeX — For JabRef, BibDesk, and other BibTeX-specific software

Include:

Citation for the content below
Citation and abstract for the content below

Purification and Characterization of the α-Glucosidase Produced by Thermophilic Fungus Thermoascus aurantiacus CBMAI 756

J. Microbiol. 2010;48(4):452-459. Published online August 20, 2010

DOI: https://doi.org/10.1007/s12275-010-9319-2

Related articles