Differential Expression Profiles of Alternaria alternate Genes in Response to Carbonyl Sulfide Fumigation

Tao Liu; Li Li; Yuejin Wang; Guoping Zhan; Bo Liu

doi:10.1007/s12275-010-9301-z

Articles

Page Path: HOME > J. Microbiol > Volume 48(4); 2010 > Article

Research Support, Non-U.S. Gov't
Differential Expression Profiles of Alternaria alternate Genes in Response to Carbonyl Sulfide Fumigation: Tao Liu , Li Li , Yuejin Wang , Guoping Zhan , Bo Liu; Journal of Microbiology 2010;48(4):480-485.
DOI: https://doi.org/10.1007/s12275-010-9301-z
Published online: August 20, 2010

Chinese Academy of Inspection and Quarantine, NO.241, Huixinxijie, Chaoyang district, Beijing 100029, P. R. China

Corresponding author: Yuejin Wang , Tel: +86-10-6493-4647,

Received: 27 September 2009 • Accepted: 11 May 2010

prev next

71 Views
0 Download
5 Crossref
5 Scopus

Full Article

Abstract

Carbonyl sulfide (COS) is a new fumigant used in phytosanitary treatments. It was developed as a potential alternative to methyl bromide, which is being phased out because of its ozone-depletion properties. To understand the molecular and cellular mechanisms occurring in fungal pathogens in response to COS fumigation, we cloned 510 cDNA fragments of Alternaria alternata (Fr.) Keissler genes that are differentially expressed; these genes were cloned using suppression subtractive hybridization. Changes in the levels of transcripts of 79 fragments were confirmed by microarray analysis and qRT-PCR. Further homology search revealed that they are highly homologous to 41 genes of other fungi, which were related to general metabolism, growth and division, defense, cellular transport, and signal transduction. These results provide an overview of differential expression profiles of A. alternata genes following COS treatment and some new clues about the mechanism of COS fungitoxicity.

Keywords: carbonyl sulfide;A. alternata;suppression subtractive hybridization (SSH);expression profiles

Figure & Data

References

Citations

Citations to this article as recorded by

Potential inhibitors of methionine aminopeptidase type II identified via structure-based pharmacophore modeling
Safana Albayati, Abdullahi Ibrahim Uba, Kemal Yelekçi
Molecular Diversity.2022; 26(2): 1005. CrossRef
Transcriptional inhibition of the Catalase gene in phosphine-induced oxidative stress in Drosophila melanogaster
Tao Liu, Li Li, Fanhua Zhang, Yuejin Wang
Pesticide Biochemistry and Physiology.2015; 124: 1. CrossRef
Analysis of a suppressive subtractive hybridization library of Alternaria alternata resistant to 2-propenyl isothiocyanate
Heriberto García-Coronado, Rosalba Troncoso-Rojas, Martín Ernesto Tiznado-Hernández, María del Carmen de la Cruz Otero, Sylvia Páz Díaz-Camacho, María Elena Báez-Flores
Electronic Journal of Biotechnology.2015; 18(4): 320. CrossRef
Sulfur Fertilization and Fungal Infections Affect the Exchange of H2S and COS from Agricultural Crops
Elke Bloem, Silvia Haneklaus, Jürgen Kesselmeier, Ewald Schnug
Journal of Agricultural and Food Chemistry.2012; 60(31): 7588. CrossRef
Sulfurous Gases As Biological Messengers and Toxins: Comparative Genetics of Their Metabolism in Model Organisms
Neal D. Mathew, David I. Schlipalius, Paul R. Ebert
Journal of Toxicology.2011; 2011: 1. CrossRef

Cite this Article

Cite this Article: export Copy Download Format; Close

Download Citation

Download a citation file in RIS format that can be imported by all major citation management software, including EndNote, ProCite, RefWorks, and Reference Manager.

Format:

RIS — For EndNote, ProCite, RefWorks, and most other reference management software
BibTeX — For JabRef, BibDesk, and other BibTeX-specific software

Include:

Citation for the content below
Citation and abstract for the content below

Differential Expression Profiles of Alternaria alternate Genes in Response to Carbonyl Sulfide Fumigation

J. Microbiol. 2010;48(4):480-485. Published online August 20, 2010

DOI: https://doi.org/10.1007/s12275-010-9301-z

Related articles