Genetic Diversity of Chromosomal Metallo-β-Lactamase Genes in Clinical Isolates of Elizabethkingia meningoseptica from Korea

Jong Hwa Yum; Eun Young Lee; Sung-Ho Hur; Seok Hoon Jeong; Hyukmin Lee; Dongeun Yong; Yunsop Chong; Eun-Woo Lee; Patrice Nordmann; Kyungwon Lee

doi:10.1007/s12275-010-9308-5

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Genetic Diversity of Chromosomal Metallo-β-Lactamase Genes in Clinical Isolates of Elizabethkingia meningoseptica from Korea: Jong Hwa Yum ¹, Eun Young Lee ², Sung-Ho Hur ³, Seok Hoon Jeong ², Hyukmin Lee ⁴, Dongeun Yong ², Yunsop Chong ², Eun-Woo Lee ⁵, Patrice Nordmann ⁶, Kyungwon Lee ²; Journal of Microbiology 2010;48(3):358-364.
DOI: https://doi.org/10.1007/s12275-010-9308-5
Published online: June 23, 2010

¹Department of Clinical Laboratory Science, Dong-eui University, Busan 614-714, Republic of Korea, ²Department of Laboratory Medicine and Research Institute of Bacterial Resistance, Yonsei University College of Medicine, Seoul 120-749, Republic of Korea, ³Department of Clinical Laboratory Science, Dong-eui Institute of Technology University, Busan 614-715, Republic of Korea, ⁴Department of Laboratory Medicine, Kwandong University College of Medicine, Goyang 412-270, Republic of Korea, ⁵Department of Life Science and Biotechnology, Dong-eui University, Busan 614-714, Republic of Korea, ⁶Service de Bacteriologie-Virologie, Hopital de Bicetre, Le Kremlin-Bicetre Cedex, France

Corresponding author: Seok Hoon Jeong , Tel: +82-2-2228-2448,

Received: 8 October 2009 • Accepted: 28 January 2010

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Abstract

This study was performed to characterize the chromosomal metallo-β-lactamases (MBLs) of Elizabethkingia meningoseptica isolated from Korea and to propose a clustering method of BlaB and GOB MBLs based on their amino acid similarities. Chromosomal MBL genes were amplified by PCR from 31 clinical isolates of E. meningoseptica. These PCR products were then cloned into a vector and electrotransformed into E. coli DH5α. Nucleotide sequencing was performed by the dideoxy chain termination method using PCR products or cloned DNA fragments. Antimicrobial susceptibilities were determined by the agar dilution method. PCR experiments showed that all 31 E. meningoseptica isolates contained both the blaB and the blaGOB genes. DNA sequence analysis revealed that E. meningoseptica isolates possessed seven types of blaB gene, including five novel variants (blaB-9 to blaB-13) and 11 types of blaGOB gene, including 10 novel variants (blaGOB-8 to blaGOB-17). The most common combination of MBL was BlaB-12 plus GOB-17 (n=19). Minimum inhibitory concentrations of imipenem and meropenem for the electrotransformants harboring novel BlaB and GOB MBLs were two- or four-fold higher than those for the recipient E. coli DH5α. BlaB and GOB MBLs were grouped in three and six clusters including fifteen novel variants, respectively, based on amino acid similarities.

Keywords: E. meningoseptica;metallo-β-lactamase;GOB;BlaB;cluster

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Genetic Diversity of Chromosomal Metallo-β-Lactamase Genes in Clinical Isolates of Elizabethkingia meningoseptica from Korea

J. Microbiol. 2010;48(3):358-364. Published online June 23, 2010

DOI: https://doi.org/10.1007/s12275-010-9308-5

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