Functional Analysis of the Inhibitor of Apoptosis Genes in Antheraea pernyi Nucleopolyhedrovirus

Feng Yan; Xiaobei Deng; Junpeng Yan; Jiancheng Wang; Lunguang Yao; Songya lv; Yipeng Qi; Hua Xu

doi:10.1007/s12275-010-9108-y

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Functional Analysis of the Inhibitor of Apoptosis Genes in Antheraea pernyi Nucleopolyhedrovirus: Feng Yan ^1,2, Xiaobei Deng ², Junpeng Yan ², Jiancheng Wang ³, Lunguang Yao ³, Songya lv ², Yipeng Qi ², Hua Xu ¹; Journal of Microbiology 2010;48(2):199-205.
DOI: https://doi.org/10.1007/s12275-010-9108-y
Published online: May 1, 2010

¹Department of Urology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Hubei, Wuhan 430030, P. R. China, ²State Key Laboratory of Virology, College of life science, Wuhan University, Wuhan, Hubei 430072, P. R. China, ³Funiushan Henan Key Laboratory of Insect Biology, Nanyang Normal University, Nanyang, Henan 473061, P. R. China

Corresponding author: Hua Xu , Tel: +82-2-380-2134,

Received: 6 April 2009 • Accepted: 31 August 2009

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Abstract

The inhibitor of apoptosis proteins (IAP) plays an important role in cell apoptosis. We cloned two novel IAP family members, Ap-iap1 and Ap-iap2, from Antheraea pernyi nucleopolyhedrovirus (ApNPV) genome. Ap-IAP1 contains two baculoviral IAP repeat (BIR) domains followed by a RING domain, but Ap-IAP2 has only one BIR domain and RING. The result of transient expression in Spodoptera frugiperda (Sf21) showed that Ap-iap1 blocked cell apoptosis induced by actinomycin D treatment and also rescued the p35 deficient Autographa californica nucleopolyhedrovirus (AcNPV) to replicate in Sf9 cells, while Ap-iap2 does not have this function. Several Ap-IAP1 truncations were constructed to test the activity of BIRs or RING motif to inhibit cell apoptosis. The results indicated that BIRs or RING of Ap-IAP1 had equally function to inhibit cell apoptosis. Therefore deletion of above both of the above domains could not block apoptosis induced by actinomycin D or rescue the replication of AcMNPV△p35. We also screened two phage-display peptides that might interact with Ap-IAP1.

Keywords: apoptosis;ApNPV;iap;phage display

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Functional Analysis of the Inhibitor of Apoptosis Genes in Antheraea pernyi Nucleopolyhedrovirus

J. Microbiol. 2010;48(2):199-205. Published online May 1, 2010

DOI: https://doi.org/10.1007/s12275-010-9108-y

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