NOTE] Biosynthesis of Poly(3-Hydroxybutyrate-co-3-Hydroxyvalerate) Copolyesters with a High Molar Fraction of 3-Hydroxyvalerate by an Insect-Symbiotic Burkholderia sp. IS-01

Do Young Kim; Doo-Sang Park; Soon Bum Kwon; Moon Gyu Chung; Kyung Sook Bae; Ho-Yong Park; Young Ha Rhee

doi:10.1007/s12275-009-0109-7

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NOTE] Biosynthesis of Poly(3-Hydroxybutyrate-co-3-Hydroxyvalerate) Copolyesters with a High Molar Fraction of 3-Hydroxyvalerate by an Insect-Symbiotic Burkholderia sp. IS-01: Do Young Kim ¹, Doo-Sang Park ², Soon Bum Kwon ³, Moon Gyu Chung ³, Kyung Sook Bae ², Ho-Yong Park ¹, Young Ha Rhee ³; Journal of Microbiology 2009;47(5):651-656.
DOI: https://doi.org/10.1007/s12275-009-0109-7
Published online: October 24, 2009

¹Industrial Bio-materials Research Center, KRIBB, Daejeon 305-806, Republic of Korea, ²Biological Resources Center, KRIBB, Daejeon 305-806, Republic of Korea, ³Department of Microbiology, Chungnam National University, Daejeon 305-764, Republic of Korea

Corresponding author: Young Ha Rhee , Tel: 82-42-821-6413,

Received: 7 April 2009 • Accepted: 22 May 2009

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Abstract

Burkholderia sp. IS-01 capable of biosynthesizing poly(3-hydroxybutyrate-co-3-hydroxyvalerate) [poly(3HB- co-3HV)] copolyesters with a high molar fraction of 3HV was isolated from the gut of the adult longicorn beetle, Moechotypa diphysis. The strain IS-01 was relatively tolerant to high concentrations of levulinic acid and accumulated a poly(13.5 mol% 3HB-co-86.5 mol% 3HV) copolyester when cultivated on a mixture of gluconate (20 g/L) and levulinic acid (12.5 g/L). In this case, the content of the copolyester in the cells was approximately 60.0%. The compositions of the copolyesters were easily regulated by altering the molar ratio of gluconate and levulinic acid in the medium. The organism was found to possess a class I PHA synthase (PhaC) gene (1,881 bp) that encodes a protein with a deduced molecular mass of 68,538 Da that consists of 626 amino acids. The PhaC of this organism was most similar to that of B. cenocepacia PC184 (92% similarity).

Keywords: Burkholderia;insect-symbiotic microorganism;levulinic acid;polyhydroxyalkanoates (PHAs);poly(3-hydroxybutyrate-co-3-hydroxyvalerate) [poly(3HB-co-3HV)];PHA synthase

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NOTE] Biosynthesis of Poly(3-Hydroxybutyrate-co-3-Hydroxyvalerate) Copolyesters with a High Molar Fraction of 3-Hydroxyvalerate by an Insect-Symbiotic Burkholderia sp. IS-01

J. Microbiol. 2009;47(5):651-656. Published online October 24, 2009

DOI: https://doi.org/10.1007/s12275-009-0109-7

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