Helicobacter pylori Proteins Response to Nitric Oxide Stress

Wei Qu; Yabin Zhou; Chunhong Shao; Yundong Sun; Qunye Zhang; Chunyan Chen; Jihui Jia

doi:10.1007/s12275-008-0266-0

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Research Support, Non-U.S. Gov't
Helicobacter pylori Proteins Response to Nitric Oxide Stress: Wei Qu ¹, Yabin Zhou ¹, Chunhong Shao ^1,2, Yundong Sun ¹, Qunye Zhang ¹, Chunyan Chen ³, Jihui Jia ¹; Journal of Microbiology 2009;47(4):486-493.
DOI: https://doi.org/10.1007/s12275-008-0266-0
Published online: September 9, 2009

¹Department of Microbiology and Key Lab for Experimental Teratology of Chinese Ministry of Education, School of Medicine, Shandong University, Jinan 250012, P. R. China, ²Clinical Laboratory, Provincial Hospital Affiliated to Shandong University, Jinan 250021, P. R. China, ³Department of Hematology, Qilu Hospital, Shandong University, Jinan 250012, P. R. China

Corresponding author: Jihui Jia , Tel: 86-531-8838-2672,

Received: 6 November 2008 • Accepted: 1 April 2009

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Abstract

Helicobacter pylori is a highly pathogenic microorganism with various strategies to evade human immune responses. Nitric oxide (NO) and reactive nitrogen species (RNS) generated via nitric oxide synthase pathway are important effectors during the innate immune response. However, the mechanisms of H. pylori to survive the nitrosative stress are not clear. Here the proteomic approach has been used to define the adaptive response of H. pylori to nitrosative stress. Proteomic analysis showed that 38 protein spots were regulated by NO donor, sodium nitroprusside (SNP). These proteins were involved in protein processing, antioxidation, general stress response, and virulence, as well as some unknown functions. Particularly, some of them were participated in iron metabolism, potentially under the control of ferric uptake regulator (Fur). Real time PCR revealed that fur was induced under nitrosative stress, consistent with our deduction. One stress-related protein up-regulated under nitrosative conditions was thioredoxin reductase (TrxR). Inactivation of fur or trxR can lead to increased susceptivity to nitrosative stress respectively. These studies described the adaptive response of H. pylori to nitric oxide stress, and analyzed the relevant role of Fur regulon and TrxR in nitrosative stress management.

Keywords: Helicobacter pylori;nitrosative stress;proteomics

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Helicobacter pylori Proteins Response to Nitric Oxide Stress

J. Microbiol. 2009;47(4):486-493. Published online September 9, 2009

DOI: https://doi.org/10.1007/s12275-008-0266-0

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