MSK
- About
- Browse Articles
-
Special Issues
- Pioneering strategies for overcoming bacterial drug resistance (2026)
- Advancing microbial engineering through synthetic biology (2025)
- Host-associated microbiome (2024)
- Bacterial regulatory mechanisms for the control of complex cellular mechanisms (2023)
- Two years into COVID-19 pandemic: Where are we? (2022)
- Collections
- For Contributors
- Policies
- E-Submission
- About
- Browse Articles
-
Special Issues
- Pioneering strategies for overcoming bacterial drug resistance (2026)
- Advancing microbial engineering through synthetic biology (2025)
- Host-associated microbiome (2024)
- Bacterial regulatory mechanisms for the control of complex cellular mechanisms (2023)
- Two years into COVID-19 pandemic: Where are we? (2022)
- Collections
- Policies
- For Contributors
Search
- Page Path
- HOME > Search
- Genotoxicity, acute and subchronic oral toxicity assessments of postbiotics of Lacticaseibacillus rhamnosus IDCC 3201
- Shin-Yae Choi, Dahae Hong, Jin Seok Moon, O-Hyun Ban, Hee-Won Bae, Tae-Yoon Kim, You-Hee Cho
- Received May 8, 2026 Accepted May 20, 2026 Published online June 12, 2026
- DOI: https://doi.org/10.71150/jm.2605002 [Epub ahead of print]
- 50 View
- 5 Download
-
Abstract
PDF
Supplementary Material -
Postbiotics derived from lactic acid bacteria (LAB) have attracted growing interest as stable and potentially safer alternatives to probiotics for use in foods and health-related products. Comprehensive safety evaluation remains essential before their broader application. In this study, we assessed the safety profiles of RHT3201, a postbiotic preparation derived from Lacticaseibacillus rhamnosus IDCC 3201, through genomic, genotoxic, acute oral, and subchronic oral toxicity studies. Whole-genome analysis showed that IDCC 3201 lacks antimicrobial resistance genes and exhibits no hemolytic activity, supporting the genomic safety of the source strain. RHT3201 showed no genotoxic potential in either in vitro or in vivo assays, as evidenced by no structural or numerical chromosomal aberrations at concentrations up to 5,000 μg/ml in CHL/IU cells and no increase in micronucleated polychromatic erythrocytes, with no suppression of bone marrow erythropoiesis by oral administration of RHT3201 at doses up to 15,000 mg/kg/day using a mouse model. In rats, single oral doses of up to 15,000 mg/kg caused no mortality, treatment-related clinical signs, or gross pathological abnormalities, indicating an approximate lethal dose greater than 15,000 mg/kg. In a 90-day repeated-dose oral toxicity study, no adverse treatment-related effects were observed at doses up to 5,000 mg/kg/day. Mild liver and thyroid histopathological findings were considered adaptive and reversible. Accordingly, the no-observed-adverse-effect level was determined to be 5,000 mg/kg/day. Taken together, these findings support the safety of RHT3201 as a LAB-derived postbiotic ingredient.
- Safety Evaluation of Lactobacillus paracasei subsp. paracasei LC-01, Probiotic Bacterium
- Hao Zhang , Yu Wang , Jing Sun , Zirui Guo , Huiyuan Guo , Fazheng Ren
- J. Microbiol. 2013;51(5):633-638. Published online October 31, 2013
- DOI: https://doi.org/10.1007/s12275-013-3336-x
- 821 View
- 1 Download
- 9 Crossref
-
Abstract
PDF
- The safety of Lactobacillus paracasei subsp. paracasei LC-01 was evaluated for its use as a potential probiotic. In our in vitro study, the antibiotic resistance and the ability to produce biogenic amine were determined. The results showed that the strain was sensitive to all tested antibiotics and did not produce biogenic amine except for tyramine. The oral toxicity of this strain was evaluated in Balb/C mice. One hundred mice were divided into 10 groups. Four groups were administered 0, 108, 109, or 1010 CFU/mouse per day dissolved in saline solution respectively, for 28 days. Three groups were injected intraperitoneally with 109 CFU/mouse dissolved in saline solution, and were killed 2, 5, and 10 days after injection. The last 3 groups were injected with the vehicle as controls respectively. The results showed that oral administration of the strain had no adverse effects on mouse body weight and that there was no treatment-associated bacterial translocation. Intraperitoneal administration caused a significant translocation to liver, spleen and kidney. However, this translocation did not cause illness or death throughout the experiment. The results suggest that L. paracasei subsp. paracasei LC-01 is likely to be safe for human consumption.
-
Citations
Citations to this article as recorded by
- Genome sequence and evaluation of safety and probiotic potential of Lacticaseibacillus paracasei LC86 and Lacticaseibacillus casei LC89
Ting Chen, Yunjiao Zhao, Yixuan Fan, Yao Dong, Zhonghui Gai
Frontiers in Microbiology.2025;[Epub] CrossRef - Lactobacillus paracasei R3 Alleviates Tumor Progression in Mice with Colorectal Cancer
Tao Chen, Baoxia Li, Kangdi Zheng, Yan Liu, Zhao Zhang, Huimei Hu, Guoqiang Qian, Jianwei Jiang
Current Microbiology.2024;[Epub] CrossRef - Comprehensive genomic analysis and evaluation of in vivo and in vitro safety of Heyndrickxia coagulans BC99
Ying Wu, Zhiyi Wu, Yinyin Gao, Yixuan Fan, Yao Dong, Yinan Zhang, Zhonghui Gai, Shaobin Gu
Scientific Reports.2024;[Epub] CrossRef - Assessment of the Safety and Probiotic Properties of Enterococcus faecium B13 Isolated from Fermented Chili
Jingmin Xiao, Cai Chen, Zhuxian Fu, Shumin Wang, Fan Luo
Microorganisms.2024; 12(5): 994. CrossRef - Lactic acid bacteria with a strong antioxidant function isolated from “Jiangshui,” pickles, and feces
Yue Hu, Yan Zhao, Xu Jia, Dan Liu, Xinhe Huang, Cheng Wang, Yanhua Zhu, Changwu Yue, Shanshan Deng, Yuhong Lyu
Frontiers in Microbiology.2023;[Epub] CrossRef - Assessment of the Safety of Lactobacillus casei IMV B-7280 Probiotic Strain on a Mouse Model
Lazarenko L.M., Babenko L.P., Gichka S.G., Sakhno L.O., Demchenko O.M., Bubnov R.V., Sichel L.M., Spivak M.Ya.
Probiotics and Antimicrobial Proteins.2021; 13(6): 1644. CrossRef - Health benefits and technological effects of Lacticaseibacillus casei-01: An overview of the scientific literature
Tatiana Colombo Pimentel, Larissa Ramalho Brandão, Matthaws Pereira de Oliveira, Whyara Karoline Almeida da Costa, Marciane Magnani
Trends in Food Science & Technology.2021; 114: 722. CrossRef - Lactobacillus casei LC01 Regulates Intestinal Epithelial Permeability through miR-144 Targeting of OCLN and ZO1
Qiuke Hou, Yongquan Huang, Yan Wang, Liu Liao, Zhaoyang Zhu, Wenjie Zhang, Yongshang Liu, Peiwu Li, Xinlin Chen, Fengbin Liu
Journal of Microbiology and Biotechnology.2020; 30(10): 1480. CrossRef - Combinations of cereal β-glucans and probiotics can enhance the anti-inflammatory activity on host cells by a synergistic effect
Mattia Pia Arena, Pasquale Russo, Vittorio Capozzi, Ana Rascón, Giovanna E. Felis, Giuseppe Spano, Daniela Fiocco
Journal of Functional Foods.2016; 23: 12. CrossRef
- Genome sequence and evaluation of safety and probiotic potential of Lacticaseibacillus paracasei LC86 and Lacticaseibacillus casei LC89
First
Prev
TOP
