Journal of Microbiology

Full article

Safety evaluation and hypolipidemic ability of water-soluble blue pigment extracted by HPD-400 resin from Quambalaria cyanescens: Ruobing Shi, Chengzhong Wang, Nianping Xue, Zhiguo Zhang; J. Microbiol. 2025;63(11):e2412011. Published online November 30, 2025; DOI: https://doi.org/10.71150/jm.2412011

138 View
1 Download

Abstract PDF: The oral administration of synthetic drugs can effectively reduce blood lipid levels, but adverse reactions may occur. Because of this, the hypolipidemic ability of natural products has been increasingly investigated. We evaluate the safety and hypolipidemic characteristics of a water-soluble blue pigment extracted using HPD-400 resin from the fungus Quambalaria cyanescens. Hypolipidemic ability was examined by constructing a hyperlipidemia model with different doses of blue pigment (50, 100, and 200 mg/kg. mouse body weight) for 28 d. Blue pigment purity increased from 20.32% to 70.70% following treatment with HPD-400 resin. Acute toxicity tests revealed blue pigment sourced from Q. cyanescens to have no toxic effects on mouse body weight, mortality, or behavioral characteristics. Subacute toxicity tests revealed no significant differences in food intake, body weight, or organ weights between treatment groups and controls. Histopathological examination of the liver and kidney tissues of mice administered blue pigment were normal, and serum enzyme activities and blood constituents were also within normal ranges. Blue pigment can significantly reduce the weight of mice, reduce liver and kidney damage and fat accumulation. It can also reduce total cholesterol, triglyceride and low density lipoprotein cholesterol in serum and liver tissue, and increase the level of high density lipoprotein cholesterol. Reduce the levels of alanine aminotransferase, aspartate aminotransferase, alkaline phosphatase, creatinine, urea and uric acid in serum. Increase the activities of total superoxide dismutase, glutathione peroxidase and catalase in serum and liver tissue, reduce the content of malondialdehyde, and up-regulate liver lipase and lipoprotein lipase. Our work proves that blue pigment is nontoxic, has the function of reducing blood lipid, and can alleviate obesity-related symptoms by regulating lipid metabolism and oxidative stress.

Review

CRISPR-Cas technologies: Emerging tools from research to clinical application: Hana Hyeon, Soonhye Hwang, Yongyang Luo, Eunkyoung Shin, Ji-Hyun Yeom, Hong-Man Kim, Minkyung Ryu, Kangseok Lee; J. Microbiol. 2025;63(8):e2504012. Published online August 31, 2025; DOI: https://doi.org/10.71150/jm.2504012

7,313 View
110 Download

Abstract PDF: CRISPR-Cas technologies have emerged as powerful and versatile tools in gene therapy. In addition to the widely used SpCas9 system, alternative platforms including modified amino acid sequences, size-optimized variants, and other Cas enzymes from diverse bacterial species have been developed to apply this technology in various genetic contexts. In addition, base editors and prime editors for precise gene editing, the Cas13 system targeting RNA, and CRISPRa/i systems have enabled diverse and adaptable approaches for genome and RNA editing, as well as for regulating gene expression. Typically, CRISPR-Cas components are transported to the target in the form of DNA, RNA, or ribonucleoprotein complexes using various delivery methods, such as electroporation, adeno-associated viruses, and lipid nanoparticles. To amplify therapeutic efficiency, continued developments in targeted delivery technologies are required, with increased safety and stability of therapeutic biomolecules. CRISPR-based therapeutics hold an inexhaustible potential for the treatment of many diseases, including rare congenital diseases, by making permanent corrections at the genomic DNA level. In this review, we present various CRISPR-based tools, their delivery systems, and clinical progress in the CRISPR-Cas technology, highlighting its innovative prospects for gene therapy.

Research Article

Synbiotic combination of fructooligosaccharides and probiotics ameliorates the metabolic dysfunction-associated steatotic liver disease: Sang Yoon Lee, Su-Been Lee, Goo-Hyun Kwon, Seol Hee Song, Jeong Ha Park, Min Ju Kim, Jung A Eom, Kyeong Jin Lee, Sang Jun Yoon, Hyunjoon Park, Sung-Min Won, Jin-Ju Jeong, Ki-Kwang Oh, Young Lim Ham, Gwang Ho Baik, Dong Joon Kim, Satya Priya Sharma, Ki Tae Suk; J. Microbiol. 2025;63(2):e2411002. Published online February 27, 2025; DOI: https://doi.org/10.71150/jm.2411002

2,055 View
95 Download
2 Web of Science
3 Crossref

Abstract PDF

Synbiotics have become a new-age treatment tool for limiting the progression of metabolic dysfunction-associated steatotic liver disease; however, inclusive comparisons of various synbiotic treatments are still lacking. Here, we have explored and evaluated multiple synbiotic combinations incorporating three distinctive prebiotics, lactitol, lactulose and fructooligosaccharides. Of the synbiotic treatments evaluated, a combination of fructooligosaccharides and probiotics (FOS+Pro) exhibited superior protection against western diet-induced liver degeneration. This synbiotic (FOS+Pro) combination resulted in the lowest body weight gains, liver weights and liver/body weight ratios. The FOS+Pro synbiotic combination substantially alleviated liver histopathological markers and reduced serum AST and cholesterol levels. FOS+Pro ameliorated hepatic inflammation by lowering expression of proinflammatory markers including TNF-α, IL-1β, IL-6, and CCL2. FOS+Pro significantly improved steatosis by restricting the expression of lipid metabolic regulators (ACC1, FAS) and lipid transporters (CD36) in the liver. These findings are critical in suggesting that synbiotic treatments are capable of restraining western diet-induced metabolic dysfunction in the liver. Additionally, this study demonstrated that adding probiotic strains amplified the effectiveness of fructooligosaccharides but not all prebiotics.

Citations

Citations to this article as recorded by

Therapeutic Potential of Probiotics in Metabolic Dysfunction-Associated Steatohepatitis: A Comprehensive Review
Xueying Wang, Zhiying Wei, Qing Xiang, Lijie Tang, Weichun Xie
Microorganisms.2025; 13(8): 1894. CrossRef
Profiling oligosaccharide components in Polygonatum kingianum with potential anti-NAFLD activity using UPLC-Orbitrap-MS/MS technology
Hong Guo, Rui Yao, Jing Fan, Ying Wang, Lingzhi Zhang, Hua Sun, Xiaohan Guo, Jianbo Yang, Jingzhe Pu, Yazhong Zhang, Baozhong Duan, Jia Chen, Wenguang Jing, Xianlong Cheng, Feng Wei
Food Hydrocolloids for Health.2025; 8: 100248. CrossRef
Probiotics and cholesterol metabolism: new frontiers in science from intestinal microecology to cardiovascular health
Yue Li, Dayong Ren
Food Science of Animal Products.2025; 4(1): 9240146. CrossRef

Journal Articles

Lactobacillus gasseri BNR17 and Limosilactobacillus fermentum ABF21069 Ameliorate High Sucrose-Induced Obesity and Fatty Liver via Exopolysaccharide Production and β-oxidation: Yu Mi Jo, Yoon Ji Son, Seul-Ah Kim, Gyu Min Lee, Chang Won Ahn, Han-Oh Park, Ji-Hyun Yun; J. Microbiol. 2024;62(10):907-918. Published online October 17, 2024; DOI: https://doi.org/10.1007/s12275-024-00173-6

900 View
15 Download
5 Web of Science
1 Crossref

Abstract PDF

Obesity and metabolic dysfunction-associated fatty liver disease (MAFLD) are prevalent metabolic disorders with substantial global health implications that are often inadequately addressed by current treatments and may have side effects. Probiotics have emerged as promising therapeutic agents owing to their beneficial effects on gut health and metabolism. This study investigated the synergistic effects of a probiotic combination of BNR17 and ABF21069 on obesity and MAFLD in C57BL/6 mice fed a high-sucrose diet. The probiotic combination significantly reduced body weight and fat accumulation compared with the high-sucrose diet. It also alleviated elevated serum leptin levels induced by a high-sucrose diet. Histological analysis revealed a significant reduction in white adipose tissue and fatty liver in the mice treated with the probiotic combination. Furthermore, increased expression of genes related to β-oxidation, thermogenesis, and lipolysis suggested enhanced metabolic activity. The probiotic groups, particularly the BNR17 group, showed an increase in fecal exopolysaccharides, along with a tendency toward a lower expression of intestinal sugar transport genes, indicating reduced sugar absorption. Additionally, inflammatory markers in the liver tissue exhibited lower expression in the ABF21069 group than in the HSD group. Despite each strain in the combination group having distinct characteristics and functions, their combined effect demonstrated synergy in mitigating obesity and MAFLD, likely through the modulation of fecal exopolysaccharides content and improvement in lipid metabolism. These findings underscore the potential of probiotic supplementation as a promising assistant therapy for managing obesity and MAFLD and provide valuable insights into its therapeutic mechanisms in metabolic disorders.

Citations

Citations to this article as recorded by

A unique tetrasaccharide-containing anchor glycolipid of lipoteichoic acid is commonly found in Lactobacillus gasseri and Lactobacillus paragasseri
Tsukasa Shiraishi, Ryosuke Kutomi, Yamaha Sato, Akihito Endo, Satoru Fukiya, Satoshi Takahashi, Atsushi Yokota, Shin-ichi Yokota
Bioscience, Biotechnology, and Biochemistry.2025; 89(9): 1382. CrossRef

Genome information of the cellulolytic soil actinobacterium Isoptericola dokdonensis DS-3 and comparative genomic analysis of the genus Isoptericola: Yurim Bae , Sujin Lee , Kitae Kim , Hyun-Kwon Lee , Soon-Kyeong Kwon , Jihyun F. Kim; J. Microbiol. 2021;59(11):1010-1018. Published online November 1, 2021; DOI: https://doi.org/10.1007/s12275-021-1452-6

348 View
0 Download
4 Web of Science
4 Crossref

Abstract PDF

The actinobacterial group is regarded as a reservoir of biologically active natural products and hydrolytic enzymes with the potential for biomedical and industrial applications. Here, we present the complete genome sequence of Isoptericola dokdonensis DS-3 isolated from soil in Dokdo, small islets in the East Sea of Korea. This actinomycete harbors a large number of genes encoding carbohydrate-degrading enzymes, and its activity to degrade carboxymethyl cellulose into glucose was experimentally evaluated. Since the genus Isoptericola was proposed after reclassification based on phylogenetic analysis, strains of Isoptericola have been continuously isolated from diverse environments and the importance of this genus in the ecosystem has been suggested by recent culturomic or metagenomic studies. The phylogenic relationships of the genus tended to be closer among strains that had been isolated from similar habitats. By analyzing the properties of published genome sequences of seven defined species in the genus, a large number of genes for carbohydrate hydrolysis and utilization, as well as several biosynthetic gene clusters for secondary metabolites, were identified. Genomic information of I. dokdonensis DS-3 together with comparative analysis of the genomes of Isoptericola provides insights into understanding this actinobacterial group with a potential for industrial applications.

Citations

Citations to this article as recorded by

Genomic analysis of Isoptericola halotolerans SM2308 reveals its potential involved in fucoidan degradation
Yu-Qi Zhang, Qi Yuan, Ji-Qing Liu, Xiao-Chen Liang, Jing-Ping Wang, Wen-Xin Jiang, Ping-Yi Li
Marine Genomics.2025; 79: 101165. CrossRef
Assessing hydrocarbon degradation capacity of Isoptericola peretonis sp. nov. and related species: a comparative study
Àngela Vidal-Verdú, Adriel Latorre-Pérez, Javier Pascual, Ruth Mañes-Collado, Aitana Nevot-Terraes, Manuel Porcar
Frontiers in Microbiology.2025;[Epub] CrossRef
From lignocellulosic biomass to single cell oil for sustainable biomanufacturing: Current advances and prospects
Yu Duan, Limei Chen, Longxue Ma, Farrukh Raza Amin, Yida Zhai, Guofu Chen, Demao Li
Biotechnology Advances.2024; 77: 108460. CrossRef
A comprehensive review on strategic study of cellulase producing marine actinobacteria for biofuel applications
Ashwini John J, Melvin S. Samuel, Muthusamy Govarthanan, Ethiraj Selvarajan
Environmental Research.2022; 214: 114018. CrossRef

Limiting the pathogenesis of Salmonella Typhimurium with berry phenolic extracts and linoleic acid overproducing Lactobacillus casei: Zajeba Tabashsum , Mengfei Peng , Cassendra Bernhardt , Puja Patel , Michael Carrion , Shaik O. Rahaman , Debabrata Biswas; J. Microbiol. 2020;58(6):489-498. Published online April 22, 2020; DOI: https://doi.org/10.1007/s12275-020-9545-1

344 View
0 Download
7 Web of Science
5 Crossref

Abstract PDF

The growing threat of emergent multidrug-resistant enteric bacterial pathogens, and their adopted virulence properties are directing to find alternative antimicrobials and/or development of dietaries that can improve host gut health and/or defense. Recently, we found that modified Lactobacillus casei (Lc + CLA) with increased production of conjugated linoleic acid has antimicrobial and other beneficial properties. Further, prebiotic alike products such as berry pomace extracts (BPEs), increase the growth of probiotics and inhibit the growth of certain bacterial pathogens. In this study, we evaluated the antibacterial effect of genetically modified Lc + CLA along with BPEs against major enteric pathogen Salmonella enterica serovar Typhimurium (ST). In mixed culture condition, the growth of ST was significantly reduced in the presence of Lc + CLA and/or BPEs. Bacterial cell-free cultural supernatant (CFCS) collected from wild-type Lc or modified Lc + CLA strains also inhibited the growth and survival of ST, and those inhibitory effects were enhanced in the presence of BPEs. We also found that the interaction of the pathogen with cultured host (HD-11 and INT-407) cells were also altered in the presence of either Lc or Lc + CLA strain or their CFCSs significantly. Furthermore, the relative expression of genes related to ST virulence and physicochemical properties of ST was altered by the effect of CFCSs of either Lc or Lc + CLA. These findings indicate that a diet containing synbiotic, specifically linoleic acid, over-produced Lc + CLA and prebiotic product BPEs, might have the potential to be effective in controlling ST growth and pathogenesis.

Citations

Citations to this article as recorded by

Natural anti-adhesive components against pathogenic bacterial adhesion and infection in gastrointestinal tract: case studies of Helicobacter pylori , Salmonella enterica , Clostridiu
Xiaoyu Bao, Jianping Wu
Critical Reviews in Food Science and Nutrition.2024; : 1. CrossRef
Combined effect of metabolites produced by a modified Lactobacillus casei and berry phenolic extract on Campylobacter and microbiome in chicken cecum contents
Zajeba Tabashsum, Zabdiel Alvarado‐Martinez, Matthew J. Wall, Arpita Aditya, Debabrata Biswas
Journal of Food Science.2023; 88(6): 2583. CrossRef
Intracellular autolytic whole cell Salmonella vaccine prevents colonization of pathogenic Salmonella Typhimurium in chicken
Mengfei Peng, Jungsoo Joo, Zabdiel Alvarado-Martinez, Zajeba Tabashsum, Arpita Aditya, Debabrata Biswas
Vaccine.2022; 40(47): 6880. CrossRef
Lactobacilli, a Weapon to Counteract Pathogens through the Inhibition of Their Virulence Factors
Andrea Colautti, Elisabetta Orecchia, Giuseppe Comi, Lucilla Iacumin, Laurie E. Comstock
Journal of Bacteriology.2022;[Epub] CrossRef
Florfenicol Enhances Colonization of a Salmonella enterica Serovar Enteritidis floR Mutant with Major Alterations to the Intestinal Microbiota and Metabolome in Neonatal Chickens
Xueran Mei, Boheng Ma, Xiwen Zhai, Anyun Zhang, Changwei Lei, Lei Zuo, Xin Yang, Changyu Zhou, Hongning Wang, Johanna Björkroth
Applied and Environmental Microbiology.2021;[Epub] CrossRef

Reviews

MINIREVIEW] Development of bacteria as diagnostics and therapeutics by genetic engineering: Daejin Lim , Miryoung Song; J. Microbiol. 2019;57(8):637-643. Published online May 11, 2019; DOI: https://doi.org/10.1007/s12275-019-9105-8

349 View
1 Download
16 Web of Science
17 Crossref

Abstract PDF

Bacteria sense and respond to the environment, communicate, and continuously interact with their surroundings, including host bodies. For more than a century, engineers have been trying to harness the natural ability of bacteria as live biotherapeutics for the treatment of diseases. Recent advances in synthetic biology facilitate the enlargement of the repertoire of genetic parts, tools, and devices that serve as a framework for biotherapy. This review describes bacterial species developed for specific diseases shown in in vitro studies and clinical stages. Here, we focus on drug delivery by programing bacteria and discuss the challenges for safety and improvement.

Citations

Citations to this article as recorded by

Modified probiotics and the related combinatorial therapeutics
Luo Zhao, Mengya Niu, Zilin Ma, Fengyun He, Xinxin Liu, Xunwei Gong, Zhanfei Chai, Ziqing Wang, Qianhua Feng, Lei Wang
Acta Pharmaceutica Sinica B.2025; 15(5): 2431. CrossRef
Applications of Nanomaterial‐Microorganism Hybrid Systems in the Treatment of Tumor
Muskan Saif Khan, Mirza Albash Baig, Meng Tian, Bowen Li, Guoqing Feng, Run Yang, Yang Bai, Bin Zheng
Advanced Therapeutics.2025;[Epub] CrossRef
Genetically engineered bacteria expressing IL-34 alleviate DSS-induced experimental colitis by promoting tight junction protein expression in intestinal mucosal epithelial cells
Weijie Chen, Tongtong Zhou, Yicun Liu, Leilei Luo, Yujing Ye, Lixian Wei, Jian Chen, Zhaolian Bian
Molecular Immunology.2025; 178: 64. CrossRef
Surface modification of probiotics at single-cell resolution: Strategies, applications, and future directions in inflammatory bowel disease
Li Peng, Xinyu Wang, Yafen Wang, Jueshuo Guo, Jianhong Yang
Materials Today Bio.2025; 35: 102485. CrossRef
Engineered Microorganisms for Advancing Tumor Therapy
Jinxuan Jia, Xiaocheng Wang, Xiang Lin, Yuanjin Zhao
Advanced Materials.2024;[Epub] CrossRef
Therapeutic bacteria and viruses to combat cancer: double-edged sword in cancer therapy: new insights for future
Aref Yarahmadi, Mitra Zare, Masoomeh Aghayari, Hamed Afkhami, Gholam Ali Jafari
Cell Communication and Signaling.2024;[Epub] CrossRef
Physiochemically and Genetically Engineered Bacteria: Instructive Design Principles and Diverse Applications
Xia Lin, Rong Jiao, Haowen Cui, Xuebing Yan, Kun Zhang
Advanced Science.2024;[Epub] CrossRef
Intestinal Delivery of Probiotics: Materials, Strategies, and Applications
Chengcheng Li, Zi‐Xi Wang, Huining Xiao, Fu‐Gen Wu
Advanced Materials.2024;[Epub] CrossRef
Research and application of intelligent diagnosis and treatment engineering bacteria
Na Zhao, Junwei Chen, Jingtian Shi, Yan Gao, Lijing Li, Liyun Dong
Frontiers in Bioengineering and Biotechnology.2024;[Epub] CrossRef
Gastrointestinal worms and bacteria: From association to intervention
James Rooney, Cinzia Cantacessi, Javier Sotillo, Alba Cortés
Parasite Immunology.2023;[Epub] CrossRef
Bacterial Therapy of Cancer: A Way to the Dustbin of History or to the Medicine of the Future?
Larisa N. Ikryannikova, Neonila V. Gorokhovets, Darya A. Belykh, Leonid K. Kurbatov, Andrey A. Zamyatnin
International Journal of Molecular Sciences.2023; 24(11): 9726. CrossRef
Derivation and elimination of uremic toxins from kidney-gut axis
Ying Xu, Wen-Di Bi, Yu-Xuan Shi, Xin-Rui Liang, Hai-Yan Wang, Xue-Li Lai, Xiao-Lu Bian, Zhi-Yong Guo
Frontiers in Physiology.2023;[Epub] CrossRef
Decorated bacteria and the application in drug delivery
Feng Wu, Jinyao Liu
Advanced Drug Delivery Reviews.2022; 188: 114443. CrossRef
Bakterie Modyfikowane Genetycznie – Perspektywy Zastosowania w Profilaktyce, Diagnostyce I Terapii
Barbara Macura, Aneta Kiecka, Marian Szczepanik
Postępy Mikrobiologii - Advancements of Microbiology.2022; 61(1): 21. CrossRef
Bacteria and cells as alternative nano-carriers for biomedical applications
Rafaela García-Álvarez, María Vallet-Regí
Expert Opinion on Drug Delivery.2022; 19(1): 103. CrossRef
Tabrizicola piscis sp. nov., isolated from the intestinal tract of a Korean indigenous freshwater fish, Acheilognathus koreensis
Jeong Eun Han, Woorim Kang, June-Young Lee, Hojun Sung, Dong-Wook Hyun, Hyun Sik Kim, Pil Soo Kim, Euon Jung Tak, Yun-Seok Jeong, Jae-Yun Lee, So-Yeon Lee, Ji-Hyun Yun, Mi-Ja Jung, Na-Ri Shin, Tae Woong Whon, Myung-Suk Kang, Ki-Eun Lee, Byoung-Hee Lee, Ji
International Journal of Systematic and Evolutionary Microbiology .2020; 70(4): 2305. CrossRef
Novel Strategies for Efficient Production and Delivery of Live Biotherapeutics and Biotechnological Uses of Lactococcus lactis: The Lactic Acid Bacterium Model
Laísa M. Tavares, Luís C. L. de Jesus, Tales F. da Silva, Fernanda A. L. Barroso, Viviane L. Batista, Nina D. Coelho-Rocha, Vasco Azevedo, Mariana M. Drumond, Pamela Mancha-Agresti
Frontiers in Bioengineering and Biotechnology.2020;[Epub] CrossRef

[MINIREVIEW] Modulation of gut microbiome in nonalcoholic fatty liver disease: pro-, pre-, syn-, and antibiotics: Min Seok Cho , Sang Yeol Kim , Ki Tae Suk , Byung-Yong Kim; J. Microbiol. 2018;56(12):855-867. Published online October 25, 2018; DOI: https://doi.org/10.1007/s12275-018-8346-2

321 View
0 Download
31 Web of Science
30 Crossref

Abstract PDF

Nonalcoholic fatty liver disease (NAFLD) is one of the most common types of liver diseases worldwide and its incidence continues to increase. NAFLD occurs when the body can no longer effectively store excess energy in the adipose tissue. Despite the increasing prevalence of NAFLD, making lifestyle changes, including increased exercise, is often an elusive goal for patients with NAFLD. The liver directly connects to the gut-gastrointestinal milieu via the portal vein, which are all part of the gut-liver axis. Therefore, the gut-microbiome and microbial products have been actively studied as likely key factors in NAFLD pathophysiology. Hence, dysbiosis of the gut microbiome and therapeutic manipulation of the gut-liver axis are being investigated. Novel therapeutic approaches for modulating gut microbiota through the administration of probiotics, prebiotics, synbiotics, and antibiotics have been proposed with numerous promising initial reports on the effectiveness and clinical applications of these approaches. This review delves into the current evidence on novel therapies that modulate gut microbiota and discusses ongoing clinical trials targeting the gut-liver axis for the management and prevention of NAFLD.

Citations

Citations to this article as recorded by

Schisandra chinensis lignans ameliorate hepatic inflammation and steatosis in methionine choline-deficient diet-fed mice by modulating the gut-liver axis
Li-Shan Yan, Jian-Ying Kang, Chun-Yu Gu, Xin-Yu Qiu, Jia-Jia Li, Brian Chi-Yan Cheng, Yi-Wei Wang, Gan Luo, Yi Zhang
Journal of Ethnopharmacology.2025; 348: 119801. CrossRef
ACG Clinical Guideline: Malnutrition and Nutritional Recommendations in Liver Disease
Ashwani K. Singal, Robert J. Wong, Srinivasan Dasarathy, Manal F. Abdelmalek, Brent A. Neuschwander-Tetri, Berkeley N. Limketkai, Jessica Petrey, Craig J. McClain
American Journal of Gastroenterology.2025; 120(5): 950. CrossRef
The crosstalk between gut microbiota and microbiota-derived metabolites in hepatocellular carcinoma
Sang Jun Yoon, Seul Ki Han, Tae Suk Kim, Ki Tae Suk, Dae Hee Choi, Young Don Kim, Moon Young Kim, Gab Jin Cheon, Soon Koo Baik, Dong Joon Kim
Critical Reviews in Microbiology.2025; : 1. CrossRef
Metabolic dysfunction-associated steatotic liver disease (MASLD): emerging insights into gut microbiota interactions and therapeutic perspectives
Wenchu Qian, Ling He, Chenxue Fu, Tiantian Zeng, Hanyu Wang, Haifang Li
Exploration of Digestive Diseases.2025;[Epub] CrossRef
Highland barley β-glucan supplementation attenuated hepatic lipid accumulation in Western diet-induced non-alcoholic fatty liver disease mice by modulating gut microbiota
Huicui Liu, Chenxi Nie, Xinzhong Hu, Juxiu Li
Food & Function.2024; 15(3): 1250. CrossRef
Therapeutic applications of melatonin in disorders related to the gastrointestinal tract and control of appetite
Atousa Moghadam Fard, Pardis Goodarzi, Mehran Mottahedi, Setareh Garousi, Hamed Zadabhari, Mohammad Kalantari Shahijan, Saeedeh Esmaeili, Mohsen Nabi-Afjadi, Bahman Yousefi
Naunyn-Schmiedeberg's Archives of Pharmacology.2024; 397(8): 5335. CrossRef
Bifidobacterium lactis Probio‐M8 prevents nonalcoholic fatty liver disease in high‐fat diet‐fed rats: The potential role in modulating gut microbiota
Wen Fan, Kairui Tang, Yuanjun Deng, Chuiyang Zheng, Maoxing Pan, Dajin Pi, Zheng Liang, Jianwei Zhen, Qinhe Yang, Yupei Zhang
Food Bioengineering.2024; 3(1): 29. CrossRef
Manipulating the Gut Microbiome to Alleviate Steatotic Liver Disease: Current Progress and Challenges
Ernesto Saenz, Nathally Espinosa Montagut, Baohong Wang, Christoph Stein-Thöringer, Kaicen Wang, Honglei Weng, Matthias Ebert, Kai Markus Schneider, Lanjuan Li, Andreas Teufel
Engineering.2024; 40: 51. CrossRef
The gut-liver axis in fatty liver disease: role played by natural products
Zhu Ming, Xie Ruishi, Xu Linyi, Yang Yonggang, Luo Haoming, Lan Xintian
Frontiers in Pharmacology.2024;[Epub] CrossRef
Dietary fiber intake and mortality among survivors of liver cirrhosis: A prospective cohort study
Zahra Hariri, Azita Hekmatdoost, Fereshteh Pashayee-khamene, Sara Karimi, Salehe Ahmadzadeh, Zahra Yari
Heliyon.2023; 9(6): e16170. CrossRef
The Activity of Prebiotics and Probiotics in Hepatogastrointestinal Disorders and Diseases Associated with Metabolic Syndrome
Alicia Rodríguez-Pastén, Nury Pérez-Hernández, Javier Añorve-Morga, Rubén Jiménez-Alvarado, Raquel Cariño-Cortés, Teresa Sosa-Lozada, Eduardo Fernández-Martínez
International Journal of Molecular Sciences.2022; 23(13): 7229. CrossRef
Non-alcoholic fatty liver disease (NAFLD) and mental illness: Mechanisms linking mood, metabolism and medicines
Anwesha Gangopadhyay, Radwa Ibrahim, Karli Theberge, Meghan May, Karen L. Houseknecht
Frontiers in Neuroscience.2022;[Epub] CrossRef
Multi-Omics Nutritional Approaches Targeting Metabolic-Associated Fatty Liver Disease
Omar Ramos-Lopez
Genes.2022; 13(11): 2142. CrossRef
Construction of two new polyoxometalate complexes and their recyclability in photodegradation of cephalexin and ceftiofur
Qian-Qian Wang, Li-Hui Mao, Da-Xiang Wang, Yan-Mei Ma, Xin-Li Shi, Xi-Hao Tian
Inorganica Chimica Acta.2022; 536: 120902. CrossRef
Gut–Liver Axis in Nonalcoholic Fatty Liver Disease: the Impact of the Metagenome, End Products, and the Epithelial and Vascular Barriers
Antonio Gil-Gómez, Paola Brescia, Maria Rescigno, Manuel Romero-Gómez
Seminars in Liver Disease.2021; 41(02): 191. CrossRef
Promising diagnostic biomarkers of nonalcoholic fatty liver disease and nonalcoholic steatohepatitis: From clinical proteomics to microbiome
Carolina Castillo-Castro, Alexandro José Martagón-Rosado, Rocio Ortiz-Lopez, Luis Felipe Garrido-Treviño, Melissa Villegas-Albo, Francisco Javier Bosques-Padilla
World Journal of Hepatology.2021; 13(11): 1494. CrossRef
Increased Prevalence of Liver Fibrosis in People Living With Human Immunodeficiency Virus Without Viral Hepatitis Compared to Population Controls
Ditte Marie Kirkegaard-Klitbo, Flemming Bendtsen, Jens Lundgren, Robert J de Knegt, Klaus Fuglsang Kofoed, Susanne Dam Nielsen, Thomas Benfield
The Journal of Infectious Diseases.2021; 224(3): 443. CrossRef
Citrulline supplementation attenuates the development of non-alcoholic steatohepatitis in female mice through mechanisms involving intestinal arginase
Dragana Rajcic, Anja Baumann, Angélica Hernández-Arriaga, Annette Brandt, Anika Nier, Cheng Jun Jin, Victor Sánchez, Finn Jung, Amélia Camarinha-Silva, Ina Bergheim
Redox Biology.2021; 41: 101879. CrossRef
Gut metabolites and inflammation factors in non-alcoholic fatty liver disease: A systematic review and meta-analysis
Xiongfeng Pan, Shi Wu Wen, Atipatsa C. Kaminga, Aizhong Liu
Scientific Reports.2020;[Epub] CrossRef
Therapeutic advances in non-alcoholic fatty liver disease: A microbiota-centered view
Hui-Ting Chen, Hong-Li Huang, Yong-Qiang Li, Hao-Ming Xu, Yong-Jian Zhou
World Journal of Gastroenterology.2020; 26(16): 1901. CrossRef
LactobacillusandPediococcusameliorate progression of non-alcoholic fatty liver disease through modulation of the gut microbiome
Na Young Lee, Sang Jun Yoon, Dae Hee Han, Haripriya Gupta, Gi Soo Youn, Min Jea Shin, Young Lim Ham, Min Jung Kwak, Byung Yong Kim, Jeong Seok Yu, Do Yup Lee, Tae-Sik Park, Si-Hyun Park, Byoung Kook Kim, Hyun Chae Joung, In Suk Choi, Ji Taek Hong, Dong Jo
Gut Microbes.2020; 11(4): 882. CrossRef
Macrophages in the pathophysiology of NAFLD: The role of sex differences
Stefano Ministrini, Fabrizio Montecucco, Amirhossein Sahebkar, Federico Carbone
European Journal of Clinical Investigation.2020;[Epub] CrossRef
IMPACT OF CURRENT DIET AT THE RISK OF NON-ALCOHOLIC FATTY LIVER DISEASE (NAFLD)
Sebastião Mauro Bezerra DUARTE, José Tadeu STEFANO, Denise Siqueira VANNI, Flair José CARRILHO, Claudia Pinto Marques Souza de OLIVEIRA
Arquivos de Gastroenterologia.2019; 56(4): 431. CrossRef
Microbiota and nonalcoholic fatty liver disease/nonalcoholic steatohepatitis (NAFLD/NASH)
Sebastião M.B. Duarte, Jose Tadeu Stefano, Claudia P. Oliveira
Annals of Hepatology.2019; 18(3): 416. CrossRef
Aged garlic extract ameliorates fatty liver and insulin resistance and improves the gut microbiota profile in a mouse model of insulin resistance
Toshio Maeda, Satomi Miki, Naoaki Morihara, Yoshiyuki Kagawa
Experimental and Therapeutic Medicine.2019;[Epub] CrossRef
Microbiota and nonalcoholic fatty liver disease
Christelle Knudsen, Audrey M. Neyrinck, Nicolas Lanthier, Nathalie M. Delzenne
Current Opinion in Clinical Nutrition & Metabolic Care.2019; 22(5): 393. CrossRef
Gold Nanoparticles Cure Bacterial Infection with Benefit to Intestinal Microflora
Juanjuan Li, Ruitao Cha, Xiaohui Zhao, Hongbo Guo, Huize Luo, Mingzheng Wang, Fengshan Zhou, Xingyu Jiang
ACS Nano.2019; 13(5): 5002. CrossRef
Role of Gut Microbiota in Hepatocarcinogenesis
Haripriya Gupta, Gi Soo Youn, Min Jea Shin, Ki Tae Suk
Microorganisms.2019; 7(5): 121. CrossRef
The Prenatal Microbiome: A New Player for Human Health
Valeria D’Argenio
High-Throughput.2018; 7(4): 38. CrossRef
Intestinal Microbiota Modulation in Obesity-Related Non-alcoholic Fatty Liver Disease
David Porras, Esther Nistal, Susana Martínez-Flórez, Javier González-Gallego, María Victoria García-Mediavilla, Sonia Sánchez-Campos
Frontiers in Physiology.2018;[Epub] CrossRef

Journal Article

Hepatitis C virus infection stimulates transforming growth factor-β1 expression through up-regulating miR-192: Ji Hyun Kim , Chang Ho Lee , Seong-Wook Lee; J. Microbiol. 2016;54(7):520-526. Published online June 28, 2016; DOI: https://doi.org/10.1007/s12275-016-6240-3

298 View
0 Download
22 Crossref

Abstract PDF

The objective of this study was to determine the molecular mechanisms underlying chronic liver injury and fibrosis caused by hepatitis C virus (HCV). This study revealed that miR-192 expression was induced by HCV infection without affecting viral replication. However, viral-induced miR-192 up-regulated transforming growth factor-β1 (TGF-β1) expression in liver cells at transcriptional level. TGF-β1 stimulation by HCV-induced miR-192 was caused through ZEB1 down-regulation and TGF-β1 increased miR-192 level via positive feedback pathway. Increase in miR-192 expression by HCV infection was due to HCV core protein released and/or expressed by viral infection. TGF-β1 promoter activity was also increased by HCV core protein in liver cells. Taken together, HCV infection resulted in increased TGF-β1 transcription in hepatocytes through ZEB1 down-regulation by HCV core-mediated miR-192 stimulation. Importantly, miR-192 inhibition with anti-miR-192 rescued ZEB1 expression down-regulated by HCV infection, thus reducing the level of TGF-β1 expression increased by HCV infection in hepatocytes. These results suggest a novel mechanism of HCV-mediated liver fibrogenesis with miR-192 being a potential molecular target to ameliorate viral pathogenesis.

Citations

Citations to this article as recorded by

Hepatitis C Virus—Core Antigen: Implications in Diagnostic, Treatment Monitoring and Clinical Outcomes
Duong Hoang Huy Le, Sitthichai Kanokudom, Ha Minh Nguyen, Ritthideach Yorsaeng, Sittisak Honsawek, Sompong Vongpunsawad, Yong Poovorawan
Viruses.2024; 16(12): 1863. CrossRef
Hepatocytes infected with hepatitis C virus change immunological features in the liver microenvironment
Soo-Jeung Park, Young S. Hahn
Clinical and Molecular Hepatology.2023; 29(1): 65. CrossRef
Cancer Stem Cell and Hepatic Stellate Cells in Hepatocellular Carcinoma
Adriana G. Quiroz Reyes, Sonia A. Lozano Sepulveda, Natalia Martinez-Acuña, Jose F. Islas, Paulina Delgado Gonzalez, Tania Guadalupe Heredia Torres, Jorge Roacho Perez, Elsa N. Garza Treviño
Technology in Cancer Research & Treatment.2023;[Epub] CrossRef
Circulating miRNA-192 and miR-29a as Disease Progression Biomarkers in Hepatitis C Patients with a Prevalence of HCV Genotype 3
Amin Ullah, Irshad Ur Rehman, Katharina Ommer, Nadeem Ahmed, Margarete Odenthal, Xiaojie Yu, Jamshaid Ahmad, Tariq Nadeem, Qurban Ali, Bashir Ahmad
Genes.2023; 14(5): 1056. CrossRef
Rotavirus-Mediated Suppression of miRNA-192 Family and miRNA-181a Activates Wnt/β-Catenin Signaling Pathway: An In Vitro Study
Anwesha Banerjee, Mamta Chawla-Sarkar, Anupam Mukherjee
Viruses.2022; 14(3): 558. CrossRef
Roles of microRNAs in Hepatitis C Virus Replication and Pathogenesis
Hui-Chun Li, Chee-Hing Yang, Shih-Yen Lo
Viruses.2022; 14(8): 1776. CrossRef
ZEB1 serves an oncogenic role in the tumourigenesis of HCC by promoting cell proliferation, migration, and inhibiting apoptosis via Wnt/β-catenin signaling pathway
Liang-yun Li, Jun-fa Yang, Fan Rong, Zhi-pan Luo, Shuang Hu, Hui Fang, Ying Wu, Rui Yao, Wei-hao Kong, Xiao-wen Feng, Bang-jie Chen, Jun Li, Tao Xu
Acta Pharmacologica Sinica.2021; 42(10): 1676. CrossRef
miRNA expression profiles in liver grafts of HCV and HIV/HCV‐infected recipients, 6 months after liver transplantation
Michela Bulfoni, Riccardo Pravisani, Emiliano Dalla, Daniela Cesselli, Masaaki Hidaka, Carla Di Loreto, Susumu Eguchi, Umberto Baccarani
Journal of Medical Virology.2021; 93(8): 4992. CrossRef
Complexation with Random Methyl-β-Cyclodextrin and (2-Hidroxypropyl)-β-Cyclodextrin Enhances In Vivo Anti-Fibrotic and Anti-Inflammatory Effects of Chrysin via the Inhibition of NF-κB and TGF-β1/Smad Signaling Pathways and Modulation of Hepatic Pro/Anti-F
Alina Ciceu, Cornel Balta, Hidegard Herman, Sami Gharbia, Simona-Rebeca Ignat, Sorina Dinescu, Judit Váradi, Ferenc Fenyvesi, Szilvia Gyöngyösi, Anca Hermenean, Marieta Costache
International Journal of Molecular Sciences.2021; 22(4): 1869. CrossRef
HCV Proteins Modulate the Host Cell miRNA Expression Contributing to Hepatitis C Pathogenesis and Hepatocellular Carcinoma Development
Devis Pascut, Minh Hoang, Nhu N. Q. Nguyen, Muhammad Yogi Pratama, Claudio Tiribelli
Cancers.2021; 13(10): 2485. CrossRef
Cell‐to‐cell spread of vaccinia virus is promoted by TGF ‐β‐independent Smad4 signalling
Anjali Gowripalan, Caitlin R. Abbott, Christopher McKenzie, Weng S. Chan, Gunasegaran Karupiah, Laurence Levy, Timothy P. Newsome
Cellular Microbiology.2020;[Epub] CrossRef
Exosomal miRNAs: Novel Players in Viral Infection
Javid Sadri Nahand, Maryam Mahjoubin-Tehran, Mohsen Moghoofei, Mohammad Hossein Pourhanifeh, Hamid Reza Mirzaei, Zatollah Asemi, Alireza Khatami, Farah Bokharaei-Salim, Hamed Mirzaei, Michael R Hamblin
Epigenomics.2020; 12(4): 353. CrossRef
Tumor necrosis family receptor superfamily member 9/tumor necrosis factor receptor-associated factor 1 pathway on hepatitis C viral persistence and natural history
Julia Peña-Asensio, Eduardo Sanz-de-Villalobos, Joaquín Miquel, Juan Ramón Larrubia
World Journal of Hepatology.2020; 12(10): 754. CrossRef
Role of non-coding RNAs in liver disease progression to hepatocellular carcinoma
Mi Jeong Heo, Jessica Yun, Sang Geon Kim
Archives of Pharmacal Research.2019; 42(1): 48. CrossRef
Mechanisms Underlying Hepatitis C Virus-Associated Hepatic Fibrosis
Mousumi Khatun, Ratna B. Ray
Cells.2019; 8(10): 1249. CrossRef
ZEB1 regulates the activation of hepatic stellate cells through Wnt/β-catenin signaling pathway
Liang-yun Li, Chen-chen Yang, Jun-fa Yang, Hao-dong Li, Bo-yu Zhang, Hong Zhou, Shuang Hu, Kun Wang, Cheng Huang, Xiao-ming Meng, Huan Zhou, Lei Zhang, Jun Li, Tao Xu
European Journal of Pharmacology.2019; 865: 172787. CrossRef
microRNAs as therapeutic targets in intestinal diseases
Chen Wang, Jiangning Chen
ExRNA.2019;[Epub] CrossRef
miRNAs regulate immune response and signaling during hepatitis C virus infection
Huange Zhu, Yan Geng, Qian He, Miaoxian Li
European Journal of Medical Research.2018;[Epub] CrossRef
Exendin-4 ameliorates high glucose-induced fibrosis by inhibiting the secretion of miR-192 from injured renal tubular epithelial cells
Yijie Jia, Zongji Zheng, Meiping Guan, Qian Zhang, Yang Li, Ling Wang, Yaoming Xue
Experimental & Molecular Medicine.2018; 50(5): 1. CrossRef
Dysregulation of cellular microRNAs by human oncogenic viruses – Implications for tumorigenesis
Joana M.O. Santos, Rui M. Gil da Costa, Rui Medeiros
Biochimica et Biophysica Acta (BBA) - Gene Regulatory Mechanisms.2018; 1861(2): 95. CrossRef
According to Hepatitis C Virus (HCV) Infection Stage, Interleukin-7 Plus 4-1BB Triggering Alone or Combined with PD-1 Blockade Increases TRAF1 low HCV-Specific CD8 + Cell Reactivity
Elia Moreno-Cubero, Dolores Subirá, Eduardo Sanz-de-Villalobos, Trinidad Parra-Cid, Antonio Madejón, Joaquín Miquel, Antonio Olveira, Alejandro González-Praetorius, Javier García-Samaniego, Juan-Ramón Larrubia, Michael S. Diamond
Journal of Virology.2018;[Epub] CrossRef
Hepatitis C virus–induced tumor‐initiating cancer stem–like cells activate stromal fibroblasts in a xenograft tumor model
Reina Sasaki, Pradip Devhare, Ratna B. Ray, Ranjit Ray
Hepatology.2017; 66(6): 1766. CrossRef

Research Support, Non-U.S. Gov't

Lactobacillus rhamnosus CCFM1107 treatment ameliorates alcohol-induced liver injury in a mouse model of chronic alcohol feeding: Fengwei Tian , Feifei Chi , Gang Wang , Xiaoming Liu , Qiuxiang Zhang , Yongquan Chen , Hao Zhang , Wei Chen; J. Microbiol. 2015;53(12):856-863. Published online December 2, 2015; DOI: https://doi.org/10.1007/s12275-015-5239-5

353 View
0 Download
51 Crossref

Abstract

Lactobacillus rhamnosus CCFM1107 was screened for high antioxidative activity from 55 lactobacilli. The present study attempted to explore the protective properties of L. rhamnosus CCFM1107 in alcoholic liver injury. A mouse model was induced by orally feeding alcohol when simultaneously treated with L. rhamnosus CCFM1107, the drug Hu-Gan- Pian (HGP), L. rhamnosus GG (LGG), and L. plantarum CCFM1112 for 3 months. Biochemical analysis was performed for both serum and liver homogenate. Detailed intestinal flora and histological analyses were also carried out. Our results indicated that the administration of L. rhamnosus CCFM1107 significantly inhibited the increase in the levels of serum aminotransferase and endotoxin, as well as the levels of triglyceride (TG) and cholesterol (CHO) in the serum and in the liver. Glutathione (GSH), glutathione peroxidase (GSH-Px) and superoxide dismutase (SOD) were elevated while the levels of malondialdehyde (MDA) were decreased. The enteric dysbiosis caused by alcohol was restored by increasing the numbers of both lactobacilli and bifidobacteria and decreasing the numbers of both enterococci and enterobacter. Histological analysis confirmed the protective effect of L. rhamnosus CCFM1107. Compared with the other lactobacilli and to the drug Hu-Gan-Pian, there is a high chance that L. rhamnosus CCFM1107 provides protective effects on alcoholic liver injury by reducing oxidative stress and restoring the intestinal flora.

Citations

Citations to this article as recorded by

Regulatory mechanisms of the probiotic-targeted gut–liver axis for the alleviation of alcohol-related liver disease: a review
Feiyu Yang, Xiangfei Li, Jing Sun, Xinyi Pang, Quancai Sun, Yingjian Lu
Critical Reviews in Food Science and Nutrition.2025; : 1. CrossRef
Weizmannia coagulans BC99 Attenuates Oxidative Stress Induced by Acute Alcoholic Liver Injury via Nrf2/SKN-1 Pathway and Liver Metabolism Regulation
Ying Wu, Cheng Li, Yinyin Gao, Jie Zhang, Yao Dong, Lina Zhao, Yuwan Li, Shaobin Gu
Antioxidants.2025; 14(1): 117. CrossRef
Immunological mechanisms and emerging therapeutic targets in alcohol-associated liver disease
Haiyuan Shen, Suthat Liangpunsakul, Yasuko Iwakiri, Gyongyi Szabo, Hua Wang
Cellular & Molecular Immunology.2025;[Epub] CrossRef
Lactobacillus plantarum and Bifidobacterium longum Alleviate Liver Injury and Fibrosis in Mice by Regulating NF-κB and AMPK Signaling
Dong-Yun Lee, Jung-Woo Shin, Yoon-Jung Shin, Seung-Won Han, Dong-Hyun Kim
Journal of Microbiology and Biotechnology.2024; 34(1): 149. CrossRef
Anti-obesity effects of Lactiplantibacillus plantarum BHP03 on high-fat diet mice and its regulatory function on intestinal microbiota
Jun Meng, Pan-Pan Dong, Meng-Xi Zhu, Zheng Zhang, Jia-Hui Chen, Yue Meng, Chang-He Ding, Han Du, Dong-Ge Zheng, Ling-Guang Du
Food Bioscience.2024; 61: 104786. CrossRef
Therapeutic Potential of Lactiplantibacillus plantarum FB091 in Alleviating Alcohol-Induced Liver Disease through Gut-Liver Axis
Soo-Jeong Lee, Jihye Yang, Gi Beom Keum, Jinok Kwak, Hyunok Doo, Sungwoo Choi, Dong-Geun Park, Chul-Hong Kim, Hyeun Bum Kim, Ju-Hoon Lee
Journal of Microbiology and Biotechnology.2024; 34(10): 2100. CrossRef
Xiaobugan decoction prevents CCl4-induced acute liver injury by modulating gut microbiota and hepatic metabolism
Weiwei Zheng, Ke Ning, Chao Shi, Yong-Fei Zhou, Yao Meng, Tong Pan, Yue Chen, Qiuhong Xie, Hongyu Xiang
Phytomedicine.2024; 135: 156113. CrossRef
The Link Between Dysbiosis, Inflammation, Oxidative Stress, and Asthma—The Role of Probiotics, Prebiotics, and Antioxidants
Paulina Kleniewska, Rafał Pawliczak
Nutrients.2024; 17(1): 16. CrossRef
Clinical efficacy of probiotics in the treatment of alcoholic liver disease: a systematic review and meta-analysis
Shi-Ying Xiong, Gui-Sheng Wu, Chun Li, Wenzhe Ma, Huai-Rong Luo
Frontiers in Cellular and Infection Microbiology.2024;[Epub] CrossRef
Lactoremediation: Heavy metals elimination from the gastrointestinal tract by lactic acid bacteria
Shokufeh Beglari, Sepideh Fereshteh, Mahnaz Milani, Niloofar Rezaie, Mahdi Rohani
Food Bioscience.2023; 56: 103202. CrossRef
Zbtb14 Promotes Non-Alcoholic Fatty Liver Disease-Associated Fibrosis in Gerbils via the β-Catenin Pathway
Guocan Chen, Xiaobing Wang, Yongfen Zhu, Huiying Hu, Xiaofeng Chu
Frontiers in Bioscience-Landmark.2023;[Epub] CrossRef
Lactobacillus plantarum-Derived Postbiotics Ameliorate Acute Alcohol-Induced Liver Injury by Protecting Cells from Oxidative Damage, Improving Lipid Metabolism, and Regulating Intestinal Microbiota
Wei Ye, Zengqiang Chen, Zhuoqi He, Haochen Gong, Jin Zhang, Jiaju Sun, Shanshan Yuan, Junjie Deng, Yanlong Liu, Aibing Zeng
Nutrients.2023; 15(4): 845. CrossRef
Harnessing the potential of probiotics in the treatment of alcoholic liver disorders
Garima Mishra, Pradeep Singh, Mulugeta Molla, Yohannes Shumet Yimer, Subas Chandra Dinda, Phool Chandra, Bhuvnesh Kumar Singh, Samuel Berihun Dagnew, Abraham Nigussie Assefa, Amien Ewunetie
Frontiers in Pharmacology.2023;[Epub] CrossRef
Bifidobacterium lactis TY-S01 protects against alcoholic liver injury in mice by regulating intestinal barrier function and gut microbiota
Xi Shu, Jing Wang, Liang Zhao, Jian Wang, Pengjie Wang, Feng Zhang, Ran Wang
Heliyon.2023; 9(7): e17878. CrossRef
Recent Perspective of Lactobacillus in Reducing Oxidative Stress to Prevent Disease
Tingting Zhao, Haoran Wang, Zhenjiang Liu, Yang Liu, DeJi, Bin Li, Xiaodan Huang
Antioxidants.2023; 12(3): 769. CrossRef
Costunolide protects against alcohol‐induced liver injury by regulating gut microbiota, oxidative stress and attenuating inflammation in vivo and in vitro
Jingxin Mao, Honghong Zhan, Fancheng Meng, Guowei Wang, Dan Huang, Zhihua Liao, Min Chen
Phytotherapy Research.2022; 36(3): 1268. CrossRef
Protective effect of flavonoids extract of Hippophae rhamnoides L. on alcoholic fatty liver disease through regulating intestinal flora and inhibiting TAK1/p38MAPK/p65NF-κB pathway
Hong Zhao, Lingzhou Kong, Mengting Shao, Jiayue Liu, Changhai Sun, Changxu Li, Yanyan Wang, Xue Chai, Yuliang Wang, Yu Zhang, Xiaoliang Li
Journal of Ethnopharmacology.2022; 292: 115225. CrossRef
Microbial treatment of alcoholic liver disease: A systematic review and meta-analysis
Qinjian Wang, Jiangmin Shi, Min Zhao, Gaoyi Ruan, Zebin Dai, Yilang Xue, Dibang Shi, Changlong Xu, Ouyue Yu, Fangyan Wang, Zhanxiong Xue
Frontiers in Nutrition.2022;[Epub] CrossRef
Fermented milk of cheese-derived Lactobacillus subsp. bulgaricus displays potentials in alleviating alcohol-induced hepatic injury and gut dysbiosis in mice
Mingzhen Liu, Miao Liu, Shenrong Yang, Caihong Shen, Xiaozhi Wang, Wenzheng Liu, Yuxing Guo
Food Research International.2022; 157: 111283. CrossRef
Pectin in Metabolic Liver Disease
Wanchao Hu, Anne-Marie Cassard, Dragos Ciocan
Nutrients.2022; 15(1): 157. CrossRef
Antioxidative and Anti‐Inflammatory Effects of Lactobacillus plantarum ZS62 on Alcohol‐Induced Subacute Hepatic Damage
Yi Gan, Xiufeng Chen, Ruokun Yi, Xin Zhao, Víctor M. Mendoza-Núñez
Oxidative Medicine and Cellular Longevity.2021;[Epub] CrossRef
Role of cell-free network communication in alcohol-associated disorders and liver metastasis
Murali R Kuracha, Peter Thomas, Martin Tobi, Benita L McVicker
World Journal of Gastroenterology.2021; 27(41): 7080. CrossRef
Hepatoprotective Effect of Lactobacillus plantarum HFY09 on Ethanol-Induced Liver Injury in Mice
Yi Gan, Jin Tong, Xianrong Zhou, Xingyao Long, Yanni Pan, Weiwei Liu, Xin Zhao
Frontiers in Nutrition.2021;[Epub] CrossRef
Regulation of Alcohol and Acetaldehyde Metabolism by a Mixture of Lactobacillus and Bifidobacterium Species in Human
Su-Jin Jung, Ji-Hyun Hwang, Eun-Ock Park, Seung-Ok Lee, Yun-Jo Chung, Myung-Jun Chung, Sanghyun Lim, Tae-Joong Lim, Yunhi Ha, Byung-Hyun Park, Soo-Wan Chae
Nutrients.2021; 13(6): 1875. CrossRef
Bifidobacterium breve ATCC15700 pretreatment prevents alcoholic liver disease through modulating gut microbiota in mice exposed to chronic alcohol intake
Xiaozhu Tian, Rong Li, Yiming Jiang, Fei Zhao, Zhengsheng Yu, Yiqing Wang, Zixing Dong, Pu Liu, Xiangkai Li
Journal of Functional Foods.2020; 72: 104045. CrossRef
Vital members in the gut microbiotas altered by two probiotic Bifidobacterium strains against liver damage in rats
Hua Zha, Dai-Qiong Fang, Aimee van der Reis, Kevin Chang, Li-Ya Yang, Jiao-Jiao Xie, Ding Shi, Qiao-Mai Xu, Ya-Ting Li, Lan-Juan Li
BMC Microbiology.2020;[Epub] CrossRef
Probiotic characteristics of Lactobacillus plantarum AR113 and its molecular mechanism of antioxidant
Xiangna Lin, Yongjun Xia, Yijin Yang, Guangqiang Wang, Wei Zhou, Lianzhong Ai
LWT.2020; 126: 109278. CrossRef
Effects of probiotic administration on hepatic antioxidative parameters depending on oxidative stress models: A meta-analysis of animal experiments
Jichun Zhao, Leilei Yu, Qixiao Zhai, Fengwei Tian, Hao Zhang, Wei Chen
Journal of Functional Foods.2020; 71: 103936. CrossRef
Bifidobacterium adolescentis and Lactobacillus rhamnosus alleviate non-alcoholic fatty liver disease induced by a high-fat, high-cholesterol diet through modulation of different gut microbiota-dependent pathways
Gang Wang, Ting Jiao, Yue Xu, Daozheng Li, Qian Si, Jianfeng Hao, Jianxin Zhao, Hao Zhang, Wei Chen
Food & Function.2020; 11(7): 6115. CrossRef
The Role of Gut-Derived Microbial Antigens on Liver Fibrosis Initiation and Progression
Dishen Chen, Thanh H. Le, Haleh Shahidipour, Scott A. Read, Golo Ahlenstiel
Cells.2019; 8(11): 1324. CrossRef
Lactobacillus plantarum LC27 and Bifidobacterium longum LC67 simultaneously alleviate high-fat diet-induced colitis, endotoxemia, liver steatosis, and obesity in mice
Hye In Kim, Jeon-Kyung Kim, Jae-Young Kim, Se-Eun Jang, Myung Joo Han, Dong-Hyun Kim
Nutrition Research.2019; 67: 78. CrossRef
Protective effects of probiotics on acute alcohol-induced liver injury in mice through alcohol metabolizing enzymes activation and hepatic TNF-α response reduction
Zhe Zhang, Hui Zhou, Lu Bai, Youyou Lv, Huaxi Yi, Lanwei Zhang, Rui Li
Journal of Functional Foods.2019; 59: 234. CrossRef
Probiotic and glutamine treatments attenuate alcoholic liver disease in a rat model
Huping Huang, Zhihui Lin, Yanling Zeng, Xueyan Lin, Yali Zhang
Experimental and Therapeutic Medicine.2019;[Epub] CrossRef
Lactobacillus rhamnosus GG Ameliorates Liver Injury and Hypoxic Hepatitis in Rat Model of CLP-Induced Sepsis
Lei Ding, Yihang Gong, Zhengfei Yang, Baojia Zou, Xialei Liu, Baimeng Zhang, Jian Li
Digestive Diseases and Sciences.2019; 64(10): 2867. CrossRef
Protective effect of lactobacillus plantarum on alcoholic liver injury and regulating of keap-Nrf2-ARE signaling pathway in zebrafish larvae
Yaping Liu, Xiaoqian Liu, Ying Wang, Cao Yi, Jiahui Tian, Kechun Liu, Jie Chu, Mei Li
PLOS ONE.2019; 14(9): e0222339. CrossRef
Simultaneous Amelioratation of Colitis and Liver Injury in Mice by Bifidobacterium longum LC67 and Lactobacillus plantarum LC27
Se-Eun Jang, Jin-Ju Jeong, Jeon-Kyung Kim, Myung Joo Han, Dong-Hyun Kim
Scientific Reports.2018;[Epub] CrossRef
Polysaccharide peptides from Coriolus versicolor: A multi-targeted approach for the protection or prevention of alcoholic liver disease
Yilin Ren, Yan Geng, Hedi Chen, Zhen-Ming Lu, Jin-Song Shi, Zhenghong Xu
Journal of Functional Foods.2018; 40: 769. CrossRef
Intestinal dysbiosis and permeability: the yin and yang in alcohol dependence and alcoholic liver disease
Peter Stärkel, Sophie Leclercq, Philippe de Timary, Bernd Schnabl
Clinical Science.2018; 132(2): 199. CrossRef
Lactic Acid Bacteria With Antioxidant Activities Alleviating Oxidized Oil Induced Hepatic Injury in Mice
Xiangna Lin, Yongjun Xia, Guangqiang Wang, Yijin Yang, Zhiqiang Xiong, Fang Lv, Wei Zhou, Lianzhong Ai
Frontiers in Microbiology.2018;[Epub] CrossRef
Lactobacillus plantarum LC27 and Bifidobacterium longum LC67 mitigate alcoholic steatosis in mice by inhibiting LPS-mediated NF-κB activation through restoration of the disturbed gut microbiota
Won-Gyeong Kim, Hye In Kim, Eun Kyung Kwon, Myung Joo Han, Dong-Hyun Kim
Food & Function.2018; 9(8): 4255. CrossRef
Comparative study of probiotic effects of Lactobacillus and Bifidobacteria strains on cholesterol levels, liver morphology and the gut microbiota in obese mice
Rostyslav V. Bubnov, Lidiia P. Babenko, Liudmyla M. Lazarenko, Viktoria V. Mokrozub, Oleksandr A. Demchenko, Oleksiy V. Nechypurenko, Mykola Ya. Spivak
EPMA Journal.2017; 8(4): 357. CrossRef
Influence of Synbiotics on Selected Oxidative Stress Parameters
Paulina Kleniewska, Rafał Pawliczak, Ilaria Peluso
Oxidative Medicine and Cellular Longevity.2017;[Epub] CrossRef
Hepatic Hazard Assessment of Silver Nanoparticle Exposure in Healthy and Chronically Alcohol Fed Mice
Ali Kermanizadeh, Nicklas R. Jacobsen, Martin Roursgaard, Steffen Loft, Peter Møller
Toxicological Sciences.2017; 158(1): 176. CrossRef
Lactobacillus plantarum LC27 and Bifidobacterium longum LC67 simultaneously alleviate ethanol-induced gastritis and hepatic injury in mice
Eun Kyung Kwon, Geum-Dan Kang, Won-Kyeong Kim, Myung Joo Han, Dong-Hyun Kim
Journal of Functional Foods.2017; 38: 389. CrossRef
Effects of probiotics on d -galactose-induced oxidative stress in plasma: A meta-analysis of animal models
Jichun Zhao, Fengwei Tian, Nan Zhao, Qixiao Zhai, Hao Zhang, Wei Chen
Journal of Functional Foods.2017; 39: 44. CrossRef
Organ–Organ Crosstalk and Alcoholic Liver Disease
Lauren Poole, Christine Dolin, Gavin Arteel
Biomolecules.2017; 7(3): 62. CrossRef
Anti-stress effect of the Lactobacillus pentosus strain S-PT84 in mice
Yuji NONAKA, Takayuki IZUMO, Toshihiro MAEKAWA, Hiroshi SHIBATA
Bioscience of Microbiota, Food and Health.2017; 36(3): 121. CrossRef
Targeting inflammation for the treatment of alcoholic liver disease
Ming-Jiang Xu, Zhou Zhou, Richard Parker, Bin Gao
Pharmacology & Therapeutics.2017; 180: 77. CrossRef
Idiosyncratic Drug-Induced Liver Injury: Is Drug-Cytokine Interaction the Linchpin?
Robert A. Roth, Ashley R. Maiuri, Patricia E. Ganey
The Journal of Pharmacology and Experimental Therapeutics.2017; 360(2): 368. CrossRef
Critical Roles of Kupffer Cells in the Pathogenesis of Alcoholic Liver Disease: From Basic Science to Clinical Trials
Tao Zeng, Cui-Li Zhang, Mo Xiao, Rui Yang, Ke-Qin Xie
Frontiers in Immunology.2016;[Epub] CrossRef
Heat-Killed Lactobacillus salivarius and Lactobacillus johnsonii Reduce Liver Injury Induced by Alcohol In Vitro and In Vivo
Cheng-Hung Chuang, Cheng-Chih Tsai, En-Shyh Lin, Chin-Shiu Huang, Yun-Yu Lin, Chuan-Ching Lan, Chun-Chih Huang
Molecules.2016; 21(11): 1456. CrossRef

Review

MINIREVIEW] The Role of MicroRNAs in Hepatitis C Virus Replication and Related Liver Diseases: Chang Ho Lee , Ji Hyun Kim , Seong-Wook Lee; J. Microbiol. 2014;52(6):445-451. Published online May 29, 2014; DOI: https://doi.org/10.1007/s12275-014-4267-x

333 View
0 Download
32 Crossref

Abstract PDF

Hepatitis C virus (HCV) infection is a worldwide health problem and is one of the main causes of chronic hepatitis, liver cirrhosis, and hepatocellular carcinoma (HCC). However, only limited therapeutic options and no vaccines are currently available against HCV infection. Recent studies of microRNAs (miRNAs), which are able to regulate HCV replication and its related liver diseases by directly interacting with the HCV genome or indirectly controlling virus-associated host pathways, have broadened our understanding of the HCV life cycle. HCV utilizes host cellular miRNAs and modulates expression of miRNAs in infected hepatocytes for its infection and propagation. Moreover, such miRNAs directly or indirectly alter HCV replication efficiency and induce liver diseases including liver fibrosis, cirrhosis, or HCC. Representatively, miR-122 directly modulates the HCV life cycle by increasing HCV translation and genomic RNA stability. Recently, a phase IIa clinical trial with miravirsen, an LNA form of antimiR-122 oligonucleotides, showed significant reduction in serum HCV levels in patients chronically infected with HCV with no detectible evidence of resistance. In addition to miR-122, other miRNAs involved in the regulation of HCV propagation could be targeted in strategies to modulate HCV replication and pathogenesis. In this review, we summarize the features of miRNAs critical for HCV replication and HCV-mediated liver abnormalities and briefly discuss their potential application as therapeutic reagents for the treatment of HCV infection and its related diseases.

Citations

Citations to this article as recorded by

MiR-130c-5p targets the SHVV n gene and upregulates immune cytokines (IL-6, IL-22, IL-1β) to inhibit viral replication
Jin Wei, Yan Ji, Yaqian Bai, Rui Cheng, Jiaqi Zhang, Xianqin Hu, Chi Zhang
Frontiers in Immunology.2024;[Epub] CrossRef
Research advances in serum chitinase-3-like protein 1 in liver fibrosis
Xingwei Hu, Wenhan Liu, Jianhua Liu, Bojian Wang, Xiaosong Qin
Frontiers in Medicine.2024;[Epub] CrossRef
A Review of miRNA Regulation in Japanese Encephalitis (JEV) Virus Infection
Maneesh Kumar, Ganesh Chandra Sahoo, Vidya Nand Rabi Das, Kamal Singh, Krishna Pandey
Current Pharmaceutical Biotechnology.2024; 25(5): 521. CrossRef
Cold exposure impairs extracellular vesicle swarm–mediated nasal antiviral immunity
Di Huang, Maie S. Taha, Angela L. Nocera, Alan D. Workman, Mansoor M. Amiji, Benjamin S. Bleier
Journal of Allergy and Clinical Immunology.2023; 151(2): 509. CrossRef
Circulating miRNA-192 and miR-29a as Disease Progression Biomarkers in Hepatitis C Patients with a Prevalence of HCV Genotype 3
Amin Ullah, Irshad Ur Rehman, Katharina Ommer, Nadeem Ahmed, Margarete Odenthal, Xiaojie Yu, Jamshaid Ahmad, Tariq Nadeem, Qurban Ali, Bashir Ahmad
Genes.2023; 14(5): 1056. CrossRef
Hepatocellular carcinoma, hepatitis C virus infection and miRNA involvement: Perspectives for new therapeutic approaches
Ester Badami, Rosalia Busà, Bruno Douradinha, Giovanna Russelli, Vitale Miceli, Alessia Gallo, Giovanni Zito, Pier Giulio Conaldi, Gioacchin Iannolo
World Journal of Gastroenterology.2022; 28(22): 2417. CrossRef
Host Epigenetic Alterations and Hepatitis B Virus-Associated Hepatocellular Carcinoma
Mirjam B. Zeisel, Francesca Guerrieri, Massimo Levrero
Journal of Clinical Medicine.2021; 10(8): 1715. CrossRef
MiRNAs and Cancer: Key Link in Diagnosis and Therapy
Yu Shi, Zihao Liu, Qun Lin, Qing Luo, Yinghuan Cen, Juanmei Li, Xiaolin Fang, Chang Gong
Genes.2021; 12(8): 1289. CrossRef
Circulating microRNA-155 is associated with insulin resistance in chronic hepatitis C patients
Nourhan M. El Samaloty, Zeinab A. Hassan, Zeinab M. Hefny, Dalia H.A. Abdelaziz
Arab Journal of Gastroenterology.2019; 20(1): 1. CrossRef
Integrated Analysis of miRNA and mRNA Expression Profiles in Spleen of Specific Pathogen-Free Chicken Infected with Avian Reticuloendotheliosis Virus Strain SNV
Shuo Gao, Hao Jiang, Jie Sun, Youxiang Diao, Yi Tang, Jingdong Hu
International Journal of Molecular Sciences.2019; 20(5): 1041. CrossRef
Molecular mechanisms of circular RNAs, transforming growth factor‐β, and long noncoding RNAs in hepatocellular carcinoma
Wenkang Shang, Gabriel Komla Adzika, Yujie Li, Qike Huang, Ningding Ding, Bianca Chinembiri, Mohammad Sajjad Ibn Rashid, Jeremiah Ong'achwa Machuki
Cancer Medicine.2019; 8(15): 6684. CrossRef
MicroRNA Expression in Focal Nodular Hyperplasia in Comparison with Cirrhosis and Hepatocellular Carcinoma
Gábor Lendvai, Tímea Szekerczés, Benedek Gyöngyösi, Krisztina Schlachter, Endre Kontsek, Adrián Pesti, Attila Patonai, Klára Werling, Ilona Kovalszky, Zsuzsa Schaff, András Kiss
Pathology & Oncology Research.2019; 25(3): 1103. CrossRef
gga-miR-21 modulates Mycoplasma gallisepticum (HS strain)-Induced inflammation via targeting MAP3K1 and activating MAPKs and NF-κB pathways
Yabo Zhao, Mengyun Zou, Yingfei Sun, Kang Zhang, Xiuli Peng
Veterinary Microbiology.2019; 237: 108407. CrossRef
miR-27b-mediated suppression of aquaporin-11 expression in hepatocytes reduces HCV genomic RNA levels but not viral titers
Fuminori Sakurai, Rina Hashimoto, Chieko Inoue, Keisaku Wakabayashi, Tomohito Tsukamoto, Tsutomu Imaizumi, Taracena Gandara Marcos Andres, Eiko Sakai, Kanae Itsuki, Naoya Sakamoto, Takaji Wakita, Hiroyuki Mizuguchi
Virology Journal.2019;[Epub] CrossRef
Novel Insights on Notch signaling pathways in liver fibrosis
Ming-ming Ni, Ya-rui Wang, Wen-wen Wu, Chong-cai Xia, Yi-he Zhang, Jing Xu, Tao Xu, Jun Li
European Journal of Pharmacology.2018; 826: 66. CrossRef
Non-Coding RNAs and Hepatitis C Virus-Induced Hepatocellular Carcinoma
Marie-Laure Plissonnier, Katharina Herzog, Massimo Levrero, Mirjam B. Zeisel
Viruses.2018; 10(11): 591. CrossRef
Circular RNAs: Characteristics, Function and Clinical Significance in Hepatocellular Carcinoma
Man Wang, Fei Yu, Peifeng Li
Cancers.2018; 10(8): 258. CrossRef
Upregulated microRNA-429 inhibits the migration of HCC cells by targeting TRAF6 through the NF-κB pathway
Peng Wang, Jia Cao, Shihai Liu, Huazheng Pan, Xiangping Liu, Aihua Sui, Liping Wang, Ruyong Yao, Zimin Liu, Jun Liang
Oncology Reports.2017; 37(5): 2883. CrossRef
Hepatitis C virus and atherosclerosis: A legacy after virologic cure?
M.F. Bassendine, S.U. Nielsen, S.H. Bridge, D.J. Felmlee, D.A. Sheridan, C.J. Packard, R.D. Neely
Clinics and Research in Hepatology and Gastroenterology.2017; 41(1): 25. CrossRef
The Potential of MicroRNAs as Novel Biomarkers for Transplant Rejection
Matthias Hamdorf, Satoru Kawakita, Matthew Everly
Journal of Immunology Research.2017; 2017: 1. CrossRef
The notorious R.N.A. in the spotlight - drug or target for the treatment of disease
Philipp Reautschnig, Paul Vogel, Thorsten Stafforst
RNA Biology.2017; 14(5): 651. CrossRef
Decline of miR‐124 in myeloid cells promotes regulatory T‐cell development in hepatitis C virus infection
Jun P. Ren, Lin Wang, Juan Zhao, Ling Wang, Shun B. Ning, Mohamed El Gazzar, Jonathan P. Moorman, Zhi Q. Yao
Immunology.2017; 150(2): 213. CrossRef
Protection of CD4+ T cells from hepatitis C virus infection-associated senescence via ΔNp63–miR-181a–Sirt1 pathway
Yun Zhou, Guang Y Li, Jun P Ren, Ling Wang, Juan Zhao, Shun B Ning, Ying Zhang, Jian Q Lian, Chang X Huang, Zhan S Jia, Jonathan P Moorman, Zhi Q Yao
Journal of Leukocyte Biology.2016; 100(5): 1201. CrossRef
Achieving sustained virologic response after interferon‐free hepatitis C virus treatment correlates with hepatic interferon gene expression changes independent of cirrhosis
E. G. Meissner, A. Kohli, K. Virtaneva, D. Sturdevant, C. Martens, S. F. Porcella, J. G. McHutchison, H. Masur, S. Kottilil
Journal of Viral Hepatitis.2016; 23(7): 496. CrossRef
MicroRNA-mediated interactions between host and hepatitis C virus
Hu Li
World Journal of Gastroenterology.2016; 22(4): 1487. CrossRef
Hepatitis C virus infection stimulates transforming growth factor-β1 expression through up-regulating miR-192
Ji Hyun Kim, Chang Ho Lee, Seong-Wook Lee
Journal of Microbiology.2016; 54(7): 520. CrossRef
MicroRNA-33a-5p Modulates Japanese Encephalitis Virus Replication by Targeting Eukaryotic Translation Elongation Factor 1A1
Zheng Chen, Jing Ye, Usama Ashraf, Yunchuan Li, Siqi Wei, Shengfeng Wan, Ali Zohaib, Yunfeng Song, Huanchun Chen, Shengbo Cao, R. M. Sandri-Goldin
Journal of Virology.2016; 90(7): 3722. CrossRef
Circulating MiRNA-122 Levels Are Associated with Hepatic Necroinflammation and Portal Hypertension in HIV/HCV Coinfection
Christian Jansen, Thomas Reiberger, Jia Huang, Hannah Eischeid, Robert Schierwagen, Mattias Mandorfer, Evrim Anadol, Philipp Schwabl, Carolynne Schwarze-Zander, Ute Warnecke-Eberz, Christian P. Strassburg, Jürgen K. Rockstroh, Markus Peck-Radosavljevic, M
PLOS ONE.2015; 10(2): e0116768. CrossRef
Advances, Nuances, and Potential Pitfalls When Exploiting the Therapeutic Potential of RNA Interference
M Battistella, PA Marsden
Clinical Pharmacology & Therapeutics.2015; 97(1): 79. CrossRef
Hepatitis B virus and microRNAs: Complex interactions affecting hepatitis B virus replication and hepatitis B virus-associated diseases
Jason Lamontagne
World Journal of Gastroenterology.2015; 21(24): 7375. CrossRef
Hepatitis C virus–induced reduction in miR‐181a impairs CD4+ T‐cell responses through overexpression of DUSP6
Guang Y. Li, Yun Zhou, Ruo S. Ying, Lei Shi, Yong Q. Cheng, Jun P. Ren, Jeddidiah W.D. Griffin, Zhan S. Jia, Chuan F. Li, Jonathan P. Moorman, Zhi Q. Yao
Hepatology.2015; 61(4): 1163. CrossRef
miR-141 targets ZEB2 to suppress HCC progression
Shi-Min Wu, Hong-Wu Ai, Ding-Yu Zhang, Xiao-Qun Han, Qin Pan, Feng-Ling Luo, Xiao-Lian Zhang
Tumor Biology.2014; 35(10): 9993. CrossRef

Research Support, Non-U.S. Gov't

Profiling of the Bacteria Responsible for Pyogenic Liver Abscess by 16S rRNA Gene Pyrosequencing: Yun Gyu Song , Sang Gun Shim , Kwang Min Kim , Dong-Hae Lee , Dae-Soo Kim , Sang-Haeng Choi , Jae-Young Song , Hyung-Lyun Kang , Seung-Chul Baik , Woo-Kon Lee , Myung-Je Cho , Kwang-Ho Rhee; J. Microbiol. 2014;52(6):504-509. Published online May 29, 2014; DOI: https://doi.org/10.1007/s12275-014-4241-7

362 View
0 Download
24 Crossref

Abstract PDF

Pyogenic liver abscess (PLA) is a severe disease with considerable mortality and is often polymicrobial. Understanding the pathogens that cause PLA is the basis for PLA treatment. Here, we profiled the bacterial composition in PLA fluid by pyrosequencing the 16S ribosomal RNA (rRNA) gene based on next-generation sequencing (NGS) technology to identify etiological agents of PLA and to provide information of their 16S rRNA sequences for application to DNA-based techniques in the hospital. Twenty patients with PLA who underwent percutaneous catheter drainage, abscess culture, and blood culture for isolates were included. Genomic DNAs from abscess fluids were subjected to polymerase chain reaction and pyrosequencing of the 16S rRNA gene with a 454 GS Junior System. The abscess and blood cultures were positive in nine (45%) and four (20%) patients, respectively. Pyrosequencing of 16S rRNA gene showed that 90% of the PLA fluid samples contained single or multiple genera of known bacteria such as Klebsiella, Fusobacterium, Streptococcus, Bacteroides, Prevotella, Peptostreptococcus, unassigned Enterobacteriaceae, and Dialister. Klebsiella was predominantly found in the PLA fluid samples. All samples that carried unassigned bacteria had 26.8% reads on average. We demonstrated that the occurrence of PLA was associated with eight known bacterial genera as well as unassigned bacteria and that 16S rRNA gene sequencing was more useful than conventional culture methods for accurate identification of bacterial pathogens from PLA.

Citations

Citations to this article as recorded by

Massive Sequencing of V3-V4 Hypervariable Region in Pyogenic Liver Abscesses Reveals the Presence of Unusual Bacteria Not Detected by Classical Culture Methods
Verónica Fernández-Sánchez, Estibeyesbo Said Plascencia-Nieto, Mónica Alethia Cureño-Díaz, Emilio Mariano Durán-Manuel, Aida Verónica Rodríguez-Tovar, Claudia Camelia Calzada-Mendoza, Clemente Cruz-Cruz, Miguel Ángel Loyola-Cruz, María Elizbeth Álvarez-Sá
Microorganisms.2025; 13(1): 131. CrossRef
Pyogenic liver abscess and sepsis caused by mixed anaerobic bacteria in an immunocompetent adult: a case report
Ziwen Fan, Mu Mei, Cen Chen
Frontiers in Medicine.2025;[Epub] CrossRef
Alterations of Gut Microbiota in Pyogenic Liver Abscess Patients with and without Type 2 Diabetes Mellitus
Shanshan Liu, Xiaomeng Ge, Xizhan Xu, Kang Zhang, Xinxin Lu
Infection and Drug Resistance.2024; Volume 17: 2149. CrossRef
Absceso hepático
G. Rossi, M. Ronot, V. Zarrouk, A. Lefort
EMC - Tratado de Medicina.2024; 28(3): 1. CrossRef
Comprehensive analysis of the microbiome and metabolome in pus from pyogenic liver abscess patients with and without diabetes mellitus
Yawen Guo, Hairui Wang, Zhaoyu Liu, Zhihui Chang
Frontiers in Microbiology.2023;[Epub] CrossRef
Metagenome Analysis of the Bacterial Characteristics in Invasive Klebsiella Pneumoniae Liver Abscesses
Zhijie Zhang, Hairui Wang, Yawen Guo, Zhaoyu Liu, Zhihui Chang
Frontiers in Cellular and Infection Microbiology.2022;[Epub] CrossRef
Involvement of Fusobacterium nucleatum in malignancies except for colorectal cancer: A literature review
Zhixing He, Wei Tian, Qichun Wei, Jing Xu
Frontiers in Immunology.2022;[Epub] CrossRef
Liver abscesses in the Western pediatric population
L Grossar, I Hoffman, E Sokal, X Stéphenne, P Witters
Acta Gastro Enterologica Belgica.2022; 85(3): 439. CrossRef
Characterization of abscesses from liver, pancreas and kidney using deep sequencing of the 16S rRNA gene
Joanna Małgorzata Bivand, Randi Monsen Nygaard, Øyvind Kommedal
Diagnostic Microbiology and Infectious Disease.2021; 99(3): 115277. CrossRef
Brevundimonas diminutacoinfection as source of pyogenic liver abscess
Jacob Burch, Shilpa Tatineni, Ikponmwosa Enofe, Heather Laird-Fick
BMJ Case Reports.2021; 14(5): e236235. CrossRef
Real‐time PCR versus MALDI‐TOF MS and culture‐based techniques for diagnosis of bloodstream and pyogenic infections in humans and animals
N.K. Abd El‐Aziz, A.A Gharib, E.A.A. Mohamed, A.H. Hussein
Journal of Applied Microbiology.2021; 130(5): 1630. CrossRef
Neonatal Exposure to BPA, BDE-99, and PCB Produces Persistent Changes in Hepatic Transcriptome Associated With Gut Dysbiosis in Adult Mouse Livers
Joe Jongpyo Lim, Moumita Dutta, Joseph L Dempsey, Hans-Joachim Lehmler, James MacDonald, Theo Bammler, Cheryl Walker, Terrance J Kavanagh, Haiwei Gu, Sridhar Mani, Julia Yue Cui
Toxicological Sciences.2021; 184(1): 83. CrossRef
Impact of Qualitative and Quantitative Biliary Contamination Status on the Incidence of Postoperative Infection Complications in Patients Undergoing Pancreatoduodenectomy
Masaya Suenaga, Yukihiro Yokoyama, Tsutomu Fujii, Suguru Yamada, Junpei Yamaguchi, Masamichi Hayashi, Takashi Asahara, Masato Nagino, Yasuhiro Kodera
Annals of Surgical Oncology.2021; 28(1): 560. CrossRef
Glycan cross-feeding supports mutualism between Fusobacterium and the vaginal microbiota
Kavita Agarwal, Lloyd S. Robinson, Somya Aggarwal, Lynne R. Foster, Ariel Hernandez-Leyva, Hueylie Lin, Brett A. Tortelli, Valerie P. O’Brien, Liza Miller, Andrew L. Kau, Hilary Reno, Nicole M. Gilbert, Warren G. Lewis, Amanda L. Lewis, Ken Cadwell
PLOS Biology.2020; 18(8): e3000788. CrossRef
Clinical characteristics and treatment outcomes in a cohort of patients with pyogenic and amoebic liver abscess
Lorna Neill, Frances Edwards, Simon M. Collin, David Harrington, Dominic Wakerley, Guduru Gopal Rao, Alastair C. McGregor
BMC Infectious Diseases.2019;[Epub] CrossRef
Profiling of the Causative Bacteria in Infected Lymphocysts after Lymphadenectomy for Gynecologic Cancer by Pyrosequencing the 16S Ribosomal RNA Gene Using Next-Generation Sequencing Technology
Yuya Nogami, Kouji Banno, Masataka Adachi, Haruko Kunitomi, Yusuke Kobayashi, Eiichiro Tominaga, Daisuke Aoki
Infectious Diseases in Obstetrics and Gynecology.2019; 2019: 1. CrossRef
PCR Characterization of Microbiota on Contracted and Non-Contracted Breast Capsules
Yara Bachour, Linda Poort, Stephan P. Verweij, Gijs van Selms, Hay A. H. Winters, Marco J. P. F. Ritt, Frank B. Niessen, Andries E. Budding
Aesthetic Plastic Surgery.2019; 43(4): 918. CrossRef
Detection of bacterial DNA from central venous catheter removed from patients by next generation sequencing: a preliminary clinical study
Ken-ichi Okuda, Yutaka Yoshii, Satomi Yamada, Akio Chiba, Ippei Hironaka, Seiji Hori, Katsuhiko Yanaga, Yoshimitsu Mizunoe
Annals of Clinical Microbiology and Antimicrobials.2018;[Epub] CrossRef
Rapid Communication: 16S ribosomal ribonucleic acid characterization of liver abscesses in feedlot cattle from three states in the United States1
M. D. Weinroth, C. R. Carlson, J. N. Martin, J. L. Metcalf, P. S. Morley, K. E. Belk
Journal of Animal Science.2017; 95(10): 4520. CrossRef
Fusobacterium nucleatum in gastroenterological cancer: Evaluation of measurement methods using quantitative polymerase chain reaction and a literature review
Kensuke Yamamura, Yoshifumi Baba, Keisuke Miyake, Kenichi Nakamura, Hironobu Shigaki, Kosuke Mima, Junji Kurashige, Takatsugu Ishimoto, Masaaki Iwatsuki, Yasuo Sakamoto, Yoichi Yamashita, Naoya Yoshida, Masayuki Watanabe, Hideo Baba
Oncology Letters.2017;[Epub] CrossRef
Fusobacterial liver abscess: a case report and review of the literature
Dilip Jayasimhan, Linus Wu, Paul Huggan
BMC Infectious Diseases.2017;[Epub] CrossRef
Automated Broad-Range Molecular Detection of Bacteria in Clinical Samples
Andries E. Budding, Martine Hoogewerf, Christina M. J. E. Vandenbroucke-Grauls, Paul H. M. Savelkoul, N. A. Ledeboer
Journal of Clinical Microbiology.2016; 54(4): 934. CrossRef
Fusobacterium nucleatum infections: clinical spectrum and bacteriological features of 78 cases
E. Denes, O. Barraud
Infection.2016; 44(4): 475. CrossRef
Pyrosequencing-based analysis of fecal microbial communities in three purebred pig lines
Edward Alain B. Pajarillo, Jong Pyo Chae, Marilen P. Balolong, Hyeun Bum Kim, Kang-Seok Seo, Dae-Kyung Kang
Journal of Microbiology.2014; 52(8): 646. CrossRef

First
Prev
Page of 1
Next
Last

Search