Journal of Microbiology

Research article

Rhizosphere microbiome differentiation and soil environmental drivers in two Monotropastrum species: Qian Liu, Xiaorong Chen, Xi Liu, Lingjuan Liu, Cuiting Chen, Lingling Li, Weiqing Liang, Pan Xu, Jinbao Pu; J. Microbiol. 2026;64(7):e2602009. Published online July 31, 2026; DOI: https://doi.org/10.71150/jm.2602009

59 View
4 Download

Abstract PDF Supplementary Material: This study compared the rhizosphere microbial communities of two closely related Monotropastrum species (M. humile, Mh; and M. humile var. glaberrima, Mhg) and identified key soil factors associated with their assembly. Bacterial and fungal communities were profiled by Illumina high-throughput sequencing, and soil physicochemical properties were assessed across multiple sites in Zhejiang Province, China. The bacterial communities of both species were dominated by Proteobacteria and Acidobacteriota at the phylum level, while the dominant fungal groups belonged to Ascomycota and Basidiomycota. The two plants shared several dominant bacterial genera, including Serratia, Burkholderia-Caballeronia-Paraburkholderia, and Bradyrhizobium, as well as common dominant fungal genera such as Saitozyma and Podila. Despite these similarities, species-specific enrichment patterns were observed. The rhizosphere of Mhg contained higher abundances of Acidothermus and Lactarius, whereas Mh preferentially enriched Cedecea, Klebsiella, and Russula. Bacterial communities were shaped by pH, soil organic matter (SOM), available potassium (AK), and available phosphorus (AP), whereas fungal communities were primarily influenced by pH, alkali-hydrolyzable nitrogen (AN), and SOM (p < 0.05). These results suggest that both host identity and soil properties contribute to rhizosphere microbial assembly, with clear host-associated differentiation in microbial communities. Notably, the identified host-associated microbial taxa, particularly key mycorrhizal fungi, may serve as potential microbial inoculants, providing new opportunities for the conservation and cultivation of mycoheterotrophic plants.

Article

Fresh Washed Microbiota Transplantation Alters Gut Microbiota Metabolites to Ameliorate Sleeping Disorder Symptom of Autistic Children: Nai-Hua Liu , Hong-Qian Liu , Jia-Yi Zheng , Meng-Lu Zhu , Li-Hao Wu , Hua-Feng Pan , Xing-Xiang He; J. Microbiol. 2023;61(8):741-753. Published online September 4, 2023; DOI: https://doi.org/10.1007/s12275-023-00069-x

760 View
5 Download
11 Web of Science
11 Crossref

Accumulating studies have raised concerns about gut dysbiosis associating autism spectrum disorder (ASD) and its related symptoms. However, the effect of gut microbiota modification on the Chinese ASD population and its underlying mechanism were still elusive. Herein, we enrolled 24 ASD children to perform the first course of fresh washed microbiota transplantation (WMT), 18 patients decided to participate the second course, 13 of which stayed to participate the third course, and there were 8 patients at the fourth course. Then we evaluated the effects of fresh WMT on these patients and their related symptoms. Our results found that the sleeping disorder symptom was positively interrelated to ASD, fresh WMT significantly alleviated ASD and its sleeping disorder and constipation symptoms. In addition, WMT stably and continuously downregulated Bacteroides/ Flavonifractor/Parasutterella while upregulated Prevotella_9 to decrease toxic metabolic production and improve detoxification by regulating glycolysis/myo-inositol/D-glucuronide/D-glucarate degradation, L-1,2-propanediol degradation, fatty acid β-oxidation. Thus, our results suggested that fresh WMT moderated gut microbiome to improve the behavioral and sleeping disorder symptoms of ASD via decrease toxic metabolic production and improve detoxification. Which thus provides a promising gut ecological strategy for ASD children and its related symptoms treatments.

Citations

Citations to this article as recorded by

Clinical response and risk factors of fecal microbiota transplantation in children: a systematic review and meta-analysis
Jiashun Liu, Xiao Sun, Ping Yuan, Yaya Qin, Wenhui Wu, Yuqing Fan, Yue Zhang, Lu Zou, Changjun Ren, Shangbin Li
European Journal of Pediatrics.2026;[Epub] CrossRef
Effects of gastrointestinal symptoms on the efficacy of washed microbiota transplantation in patients with autism
Dong-Xia Hu, Cai-Mei Lu, Xin-Yu Si, Qing-Ting Wu, Li-Hao Wu, Hao-Jie Zhong, Xing-Xiang He
Frontiers in Pediatrics.2025;[Epub] CrossRef
The Impact of Fecal Microbiota Transplantation on Gastrointestinal and Behavioral Symptoms in Children and Adolescents with Autism Spectrum Disorder: A Systematic Review
Anna Liber, Małgorzata Więch
Nutrients.2025; 17(13): 2250. CrossRef
Pathogenic bacteria enriched in the oral microbiota might be associated with recurrent pulmonary infections in elderly individuals
Jingyi Xu, Ruyi Qu, Keke Yang, Yuezhu Wang, Meiyun Nie, Xiaodong Qi, Huajun Zheng, Ling Yang
Aging Clinical and Experimental Research.2025;[Epub] CrossRef
Does constipation affect the effectiveness of washed microbiota transplantation in treating autism spectrum disorders?
Zihao Pan, Zheng Gao, Junyi Chen, Yongxi Quan, Jiating Xu, Xiaofeng Liang, Wenrui Xie, Xingxiang He, Lihao Wu
Frontiers in Neuroscience.2025;[Epub] CrossRef
Brain-gut-microbiota interactions in sleep disorders
Zhe Wang, Tingting Wu, Juan Li, Tangsheng Lu, Yifan Yu, Zichun Guan, Guohao Yuan, Zhaoyan Lv, Yiming Shan, Wei Yan, Xiaoxing Liu, Michael V. Vitiello, Qingqing Yin, Jie Sun, Lin Lu
Brain Medicine.2025; : 1. CrossRef
Washed microbiota transplantation: candidates for a novel strategy for ameliorating autism spectrum disorder
Shuo Feng, Jiangyan Wang, Xinyu Si, Shenghua Lu, Caimei Lu, Zheng Gao, Juan Yang, Jiali Wu, Xingxiang He, Lei Wu
Frontiers in Microbiology.2025;[Epub] CrossRef
Microbiota-based interventions for autism spectrum disorder: a systematic review of efficacy and clinical potential
Hana Taha, Ahmad Issa, Zaid Muhanna, Majdi Al-Shehab, Tuleen Wadi, Suhib Awamleh, Yousef A. Ateiwi, Mohammad Abusido, Vanja Berggren
Frontiers in Microbiology.2025;[Epub] CrossRef
Fecal Microbiota Transplantation for Autism Spectrum Disorder in Children: Results from a Prospective Open-Label Controlled Observational Study
Dominykas Varnas, Arnas Kunevičius, Aurelijus Burokas, Vaidotas Urbonas
Medicina.2025; 62(1): 65. CrossRef
Untargeted urine metabolomics and machine learning provide potential metabolic signatures in children with autism spectrum disorder
Xian Liu, Xin Sun, Cheng Guo, Zhi-Fang Huang, Yi-Ru Chen, Fang-Mei Feng, Li-Jie Wu, Wen-Xiong Chen
Frontiers in Psychiatry.2024;[Epub] CrossRef
Washed Microbiota Transplantation Improves the Sleep Quality in Patients with Inflammatory Bowel Disease
Qianqian Li, Yujie Liu, Zulun Zhang, Sheng Zhang, Xiao Ding, Faming Zhang
Nature and Science of Sleep.2024; Volume 16: 1141. CrossRef

Article

Description of Corynebacterium poyangense sp. nov., isolated from the feces of the greater white-fronted geese (Anser albifrons): Qian Liu , Guoying Fan , Kui Wu , Xiangning Bai , Xi Yang , Wentao Song , Shengen Chen , Yanwen Xiong , Haiying Chen; J. Microbiol. 2022;60(7):668-677. Published online May 25, 2022; DOI: https://doi.org/10.1007/s12275-022-2089-9

782 View
2 Download
1 Web of Science
3 Crossref

Abstract PDF

Two novel Gram-positive, non-spore-forming, facultatively anaerobic, non-motile, and short rods to coccoid strains were isolated from the feces of the greater white-fronted geese (Anser albifrons) at Poyang Lake. The 16S rRNA gene sequences of strains 4H37-19T and 3HC-13 shared highest identity to that of Corynebacterium uropygiale Iso10T (97.8%). Phylogenetic and phylogenomic analyses indicated that strains 4H37-19T and 3HC-13 formed an independent clade within genus Corynebacterium and clustered with Corynebacterium uropygiale Iso10T. The average nucleotide identity and digital DNA-DNA hybridization value between strains 4H37-19T and 3HC-13 and members within genus Corynebacterium were all below 95% and 70%, respectively. The genomic G + C content of strains 4H37-19T and 3HC-13 was 52.5%. Diphosphatidylglycerol (DPG), phosphatidylglycerol (PG), phosphatidylinositol (PI), phosphatidylcholine, and phosphatidyl inositol mannosides (PIM) were the major polar lipids, with C18:1ω9c, C16:0, and C18:0 as the major fatty acids, and MK-8 (H4), MK-8(H2), and MK-9(H2) as the predominant respiratory quinones. The major whole cell sugar was arabinose, and the cell wall included mycolic acids. The cell wall peptidoglycan contained meso-diaminopimelic acid (meso-DAP). The polyphasic taxonomic data shows that these two strains represent a novel species of the genus Corynebacterium, for which the name Corynebacterium poyangense sp. nov. is proposed. The type strain of Corynebacterium poyangense is 4H37-19T (=GDMCC 1.1738T = KACC 21671T).

Citations

Citations to this article as recorded by

Valid and accepted novel bacterial taxa isolated from non-domestic animals and taxonomic revisions published in 2023
Erik Munson, Claire R. Burbick, Sara D. Lawhon, Trinity Krueger, Elena Ruiz-Reyes, Romney M. Humphries
Journal of Clinical Microbiology.2024;[Epub] CrossRef
Validation List no. 212. Valid publication of new names and new combinations effectively published outside the IJSEM
Aharon Oren, Markus Göker
International Journal of Systematic and Evolutionary Microbiology .2023;[Epub] CrossRef
Keratokonjunktivitisli bir tavuktan Corynebacterium spp. ve Arcanobacterium spp. izolasyonu
Hüban GÖÇMEN, Banur BOYNUKARA
Veteriner Hekimler Derneği Dergisi.2023; 94(2): 161. CrossRef

Article

The cytoplasmic loops of AgrC contribute to the quorum-sensing activity of Staphylococcus aureus: Qian Huang , Yihui Xie , Ziyu Yang , Danhong Cheng , Lei He , Hua Wang , Qian Liu , Min Li; J. Microbiol. 2021;59(1):92-100. Published online November 17, 2020; DOI: https://doi.org/10.1007/s12275-021-0274-x

736 View
1 Download
10 Web of Science
10 Crossref

Abstract PDF

In Staphylococcus aureus, the accessory gene regulator (agr) quorum-sensing system is thought to play an important role in biofilm formation. The histidine kinase AgrC is one of the agr system components and activated by the self-generated auto-inducing peptide (AIP), which is released continuously into the extracellular environment during bacterial growth. The extracellular loops (Extra-loops) of AgrC are crucial for AIP binding. Here, we reported that the cytoplasmic loops (Cyto-loops) of AgrC are also involved in Agr activity. We identified S. aureus ST398 clinical isolates containing a naturally occurring single amino acid substitution (lysine to isoleucine) at position 73 of an AgrC Cyto-loop that exhibited significantly stronger biofilm formation and decreased Agr activity compared to the wild-type strain. A constructed strain containing the K73I point mutation in AgrC Cyto-loop continued to show a growth dependent induction of the agr system, although the growth dependent induction was delayed by about 6 h compared to the wild-type. In addition, a series of strains containing deletion mutants of the AgrC Cyto- and Extra-loops were constructed and revealed that the removal of the two Cyto-loops and Extra-loops 2 and 3 totally abolished the Agr activity and the growth-dependence on the agr system induction. Remarkably, the Extra-loop 1 deletion did not affect the Agr activity. In conclusion, the AgrC Cyto-loops play a crucial role in the S. aureus quorum-sensing activity.

Citations

Citations to this article as recorded by

Hijacking the signal: a critical evaluation of quorum sensing inhibitors as a next-generation approach against Staphylococcus aureus
Sama S. Eltaher, Zeina Khattab, Gina Walid, Omar Loay, Rana Emad, Clara Hakim, Mohamed Elhadidy
Cell Communication and Signaling.2026;[Epub] CrossRef
Triad warfare against biofilms: disrupting quorum sensing, nanotechnology-driven precision, and host immunomodulation to conquer antimicrobial resistance
Hamza M. A. Eid, Yahya A. Almutawif, Najeeb Ullah Khan
Archives of Microbiology.2026;[Epub] CrossRef
BREAKING BACTERIAL CODE
Vaishnavi Sonar, Sujata Dudhe, Paresh Sonawane, Laxmikant Borse
PROBLEMS of Infectious and Parasitic Diseases.2026; 54(1): 40. CrossRef
Comprehensive genomics, probiotic, and antibiofilm potential analysis of Streptococcus thermophilus strains isolated from homemade and commercial dahi
Aiswarya Sudheer, Debabrata Ghosh Dastidar, Gourav Ghosh, Zarin Taj, Illathu Kandy Nidhin, Indranil Chattopadhyay
Scientific Reports.2025;[Epub] CrossRef
Advances in research on small colony variants of Staphylococcus aureus
Zhiyi Ye, Tianchi Chen, Qi Zhang, Weiyi Huang, Lei He
The Microbe.2025; 8: 100444. CrossRef
Bioinspired programmed antibiofilm strategies for accelerated wound healing via spatiotemporally controlled enzyme nanoreactors
Juntao Hu, Yibing Huang, Hao Hao, Pujing Tian, Yinuo Yin, Yuting He, Fengjie Hao, Wantong Jiang, Yanping Zhang, Youzhong Wan, Quan Luo
Journal of Controlled Release.2025; 381: 113582. CrossRef
Emerging trends in the inhibition of bacterial molecular communication: An overview
B.R. Maha Swetha, M. Saravanan, Prakash Piruthivraj
Microbial Pathogenesis.2024; 186: 106495. CrossRef
In vitro and in silico evaluation of anti-quorum sensing activity of marine red seaweeds-Portieria hornemannii and Halymenia dilatata
Prakash Piruthivraj, B.R. Maha Swetha, A. Anita Margret, A. Sherlin Rosita, Parthasarathi Rengasamy, Rajapandiyan Krishnamoorthy, Mansour K. Gatasheh, Khalid Elfaki Ibrahim, Sekhu Ansari, Natesan Vijayakumar
Journal of King Saud University - Science.2024; 36(6): 103188. CrossRef
Commensal Staphylococcus epidermidis Defends against Staphylococcus aureus through SaeRS Two-Component System
Nadira Nurxat, Lili Wang, Qichen Wang, Shujing Li, Chen Jin, Yaran Shi, Ayjiamali Wulamu, Na Zhao, Yanan Wang, Hua Wang, Min Li, Qian Liu
ACS Omega.2023; 8(20): 17712. CrossRef
Biofilm and Small Colony Variants—An Update on Staphylococcus aureus Strategies toward Drug Resistance
Henan Guo, Yucui Tong, Junhao Cheng, Zaheer Abbas, Zhongxuan Li, Junyong Wang, Yichen Zhou, Dayong Si, Rijun Zhang
International Journal of Molecular Sciences.2022; 23(3): 1241. CrossRef

First
Prev
Page of 1
Next
Last

Search