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HOME > J. Microbiol > Volume 59(1); 2021 > Article
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The cytoplasmic loops of AgrC contribute to the quorum-sensing activity of Staphylococcus aureus
Qian Huang , Yihui Xie , Ziyu Yang , Danhong Cheng , Lei He , Hua Wang , Qian Liu , Min Li
Journal of Microbiology 2021;59(1):92-100.
DOI: https://doi.org/10.1007/s12275-021-0274-x
Published online: November 17, 2020
Department of Laboratory Medicine, RenJi Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai 200127, P. R. China
Corresponding author:  Qian Liu , Tel: +8613918363998, 
Min Li , Tel: +8613918363998, 
Received: 22 May 2020   • Revised: 22 September 2020   • Accepted: 25 September 2020
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In Staphylococcus aureus, the accessory gene regulator (agr) quorum-sensing system is thought to play an important role in biofilm formation. The histidine kinase AgrC is one of the agr system components and activated by the self-generated auto-inducing peptide (AIP), which is released continuously into the extracellular environment during bacterial growth. The extracellular loops (Extra-loops) of AgrC are crucial for AIP binding. Here, we reported that the cytoplasmic loops (Cyto-loops) of AgrC are also involved in Agr activity. We identified S. aureus ST398 clinical isolates containing a naturally occurring single amino acid substitution (lysine to isoleucine) at position 73 of an AgrC Cyto-loop that exhibited significantly stronger biofilm formation and decreased Agr activity compared to the wild-type strain. A constructed strain containing the K73I point mutation in AgrC Cyto-loop continued to show a growth dependent induction of the agr system, although the growth dependent induction was delayed by about 6 h compared to the wild-type. In addition, a series of strains containing deletion mutants of the AgrC Cyto- and Extra-loops were constructed and revealed that the removal of the two Cyto-loops and Extra-loops 2 and 3 totally abolished the Agr activity and the growth-dependence on the agr system induction. Remarkably, the Extra-loop 1 deletion did not affect the Agr activity. In conclusion, the AgrC Cyto-loops play a crucial role in the S. aureus quorum-sensing activity.

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    The cytoplasmic loops of AgrC contribute to the quorum-sensing activity of Staphylococcus aureus
    J. Microbiol. 2021;59(1):92-100.   Published online November 17, 2020
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