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Special Issues
- Pioneering strategies for overcoming bacterial drug resistance (2026)
- Advancing microbial engineering through synthetic biology (2025)
- Host-associated microbiome (2024)
- Bacterial regulatory mechanisms for the control of complex cellular mechanisms (2023)
- Two years into COVID-19 pandemic: Where are we? (2022)
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- Revealing genetic variation of Actinobacillus pleuropneumoniae Korean isolates using whole genome sequence analysis
- Eun-Seo Lee, Su Min Kyung, Jun Ho Lee, Xi-Rui Xiang, Han Sang Yoo
- Received December 16, 2025 Accepted March 3, 2026 Published online April 21, 2026
- DOI: https://doi.org/10.71150/jm.2512010 [Epub ahead of print]
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Abstract
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Supplementary Material -
Actinobacillus pleuropneumoniae (APP) is the etiological agent of porcine pleuropneumoniae (PP), a high contagious respiratory disease with significant impact on the swine industry in both clinically and economically. Despite of the several attempts to control APP, the emergence of novel serotypes and antimicrobial resistance (AMR) strains highlights the importance of monitoring the genetic characteristics of APP at single nucleotide level. Despite the importance of genomic surveillance of APP to develop effective control strategies, genetic information on the recent Korean isolates of APP is not available at whole genome level. Therefore, in this study, six APP strains were isolated from porcine lungs with characteristic lesions of PP from 2022 to 2024. And their whole genomic sequences, serotypes, virulence factors, and AMR traits were investigated using combined short- and long-read sequencing methods. In silico PCR serotyping identified the isolates as serotype 1, 7, and 15, while one isolate was non-typeable. Multiple AMR genes including Hinf_PBP3_BLA, Ecol_EFTu_PLV, tet(B), tet(O), tetR, sul2, aph(3'')-Ib, aph(6)-Id, and aph(3')-Ia were detected. Also, these genes were located with adjacent to mobile genetic elements, suggesting the possibility of horizontal gene transfer. Phylogenetic comparison with 40 global APP complete genomes, presented that Korean isolates were closely related with China and Switzerland strains. This study provides the whole genome sequences based genetic characterization on the recent Korean isolates of APP, and this study emphasizes that continuous monitoring of APP genomic variation to support effective control of porcine pleuropneumoniae.
- Dense Granule Protein-7 (GRA-7) of Toxoplasma gondii inhibits viral replication in vitro and in vivo
- Prasanna Weeratunga , Thilina U. B. Herath , Tae-Hwan Kim , Hyun-Cheol Lee , Jae-Hoon Kim , Byeong-Hoon Lee , Eun-Seo Lee , Kiramage Chathuranga , W. A. Gayan Chathuranga , Chul-Su Yang , Jin Yeul Ma , Jong-Soo Lee
- J. Microbiol. 2017;55(11):909-917. Published online October 27, 2017
- DOI: https://doi.org/10.1007/s12275-017-7392-5
- 561 View
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- 17 Crossref
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Abstract
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- Dense granule protein-7 (GRA-7) is an excretory protein of Toxoplasma gondii. It is a potential serodiagnostic marker and vaccine candidate for toxoplasmosis. Previous reports demonstrated that GRA-7 induces innate immune responses in macrophages by interacting with TRAF6 via the MyD88- dependent pathway. In the present study, we evaluated the antiviral activity and induction of an antiviral state by GRA-7 both in vitro and in vivo. It was observed that GRA-7 markedly reduced the replication of vesicular stomatitis virus (VSVGFP), influenza A virus (PR8-GFP), coxsackievirus (H3- GFP), herpes simplex virus (HSV-GFP), and adenovirus-GFP in epithelial (HEK293T/HeLa) and immune (RAW264.7) cells. These antiviral activities of GRA-7 were attributed to the induction of type I interferon (IFN) signaling, resulting in the secretion of IFNs and pro-inflammatory cytokines. Additionally, in BALB/c mice, intranasal administration of GRA-7 prevented lethal infection by influenza A virus (H1N1) and exhibited prophylactic effects against respiratory syncytial virus (RSV-GFP). Collectively, these results suggested that GRA-7 exhibits immunostimulatory and broad spectrum antiviral activities via type I IFN signaling. Thus, GRA-7 can be potentially used as a vaccine adjuvant or as a candidate drug with prophylactic potential against viruses.
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Citations
Citations to this article as recorded by
- Global research landscape and trends in Toxoplasma gondii from 2003 to 2022: A bibliometric analysis
Shi-Jie Fan, Ming Pan, Chen-Yang Xia, Pin Yang, Si-Yang Huang
Veterinary Parasitology.2025; 335: 110438. CrossRef - Study of dense granule proteins in Eimeria spp. identifies a limited repertoire with potential as vaccine candidates
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Frontiers in Immunology.2025;[Epub] CrossRef - Effect of SARS-CoV-2 and Toxoplasma gondii co-infection on IFN-γ and TNF-α expression and its impact on disease severity
Magda S.A. Abdeltawab, Mohamed Fateen, Shimaa Saad El-Din, Riem M. Elmessiery, Osama Mohammady Mohamed, Khaled Marzouk Sadek, Engy Medhat, Alshaimaa M.R. Hamed
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Folia Parasitologica.2024;[Epub] CrossRef - IFNs in host defence and parasite immune evasion during Toxoplasma gondii infections
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Frontiers in Immunology.2024;[Epub] CrossRef - Current vaccine candidate of toxoplasmosis
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Journal of Life Science and Biomedicine.2024; : 54. CrossRef - Prevalence of Toxoplasma gondii infection in COVID-19 patients: A systematic review and meta-analysis
Mahdi Fakhar, Khadijeh Najafi Ghobadi, Nastaran Barati, Salman Zafari, Seyed Ali Hosseini, Eissa Soleymani, Seyedmousa Motavallihaghi
Microbial Pathogenesis.2024; 197: 107064. CrossRef - Clinical and Immunological Impacts of Latent Toxoplasmosis on COVID-19 Patients
Samar Habib, Eman Hamza, Randa El-Gamal, Nessma A Nosser, Wafaa A Aboukamar, Sherehan Abdelsalam, Ali Sobh, Mohamed Elegezy, Mohamed Elbayoumy, Waleed Eldars, Khaled Elmasry, Marwa H Elnagdy
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Marwa A. Gouda, Hind S. AboShabaan, Ahmed S. Abdelgawad, Aliaa Sabry Abdel Wahed, Khaled A. Abd El-Razik, Yara Elsaadawy, Ayman. A. Abdel-Wahab, Yousry Hawash
Scientific Reports.2023;[Epub] CrossRef - Toxoplasmosis and symptoms severity in patients with COVID-19 in referral centers in Northern Iran
Ali Geraili, Alireza Badirzadeh, Maryam Sadeghi, Seyed Mahmoud Mousavi, Parisa Mousavi, Zabihollah Shahmoradi, Sayed-Mohsen Hosseini, Seyed Hossein Hejazi, Raheleh Rafiei-Sefiddashti
Journal of Parasitic Diseases.2023; 47(1): 185. CrossRef - Synergistic effect of GRA7 and profilin proteins in vaccination against chronic Toxoplasma gondii infection
Nadia Arcon, Mariano S. Picchio, Ignacio M. Fenoy, Rosalía E. Moretta, Ariadna S. Soto, Matías D. Perrone Sibilia, Vanesa R. Sánchez, Cecilia A. Prato, María Virginia Tribulatti, Alejandra Goldman, Valentina Martin
Vaccine.2021; 39(6): 933. CrossRef - GRA8 DNA vaccine formulations protect against chronic toxoplasmosis
Muhammet Karakavuk, Hüseyin Can, Aytül Gül, Aysu Değirmenci Döşkaya, Sedef Erkunt Alak, Cemal Ün, Adnan Yüksel Gürüz, Mert Döşkaya
Microbial Pathogenesis.2021; 158: 105016. CrossRef - Role of interferon gamma in SARS-CoV-2-positive patients with parasitic infections
Enas Fakhry Abdel-Hamed, Mohamed N. Ibrahim, Nahed E. Mostafa, Howayda S. F. Moawad, Nahla E. Elgammal, Ehab M. Darwiesh, Dina S. El-rafey, Nissreen E. ElBadawy, Emad Ali Al-Khoufi, Salwa I. Hindawi
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Kevin Roe
Microbial Pathogenesis.2021; 161: 105299. CrossRef - Preparation of colloidal gold immunochromatographic test strips for the diagnosis ofToxoplasma gondii
Yuanxi Shen, Zhaozhe Wang, Jiajing Li, Rui Xu, Rongyi Ji, Jiaojiao Lin, Chuangang Zhu
Food and Agricultural Immunology.2020; 31(1): 630. CrossRef - A negative covariation between toxoplasmosis and CoVID-19 with alternative interpretations
Łukasz Jankowiak, Lajos Rozsa, Piotr Tryjanowski, Anders Pape Møller
Scientific Reports.2020;[Epub] CrossRef - Characterization of a novel secretory spherical body protein in Babesia orientalis and Babesia orientalis-infected erythrocytes
Jiaying Guo, Muxiao Li, Yali Sun, Long Yu, Pei He, Zheng Nie, Xueyan Zhan, Yangnan Zhao, Xiaoying Luo, Sen Wang, Siqi Aoyang, Qin Liu, Cuiqin Huang, Lan He, Junlong Zhao
Parasites & Vectors.2018;[Epub] CrossRef
- Global research landscape and trends in Toxoplasma gondii from 2003 to 2022: A bibliometric analysis
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