Skip Navigation
Skip to contents

Journal of Microbiology : Journal of Microbiology

OPEN ACCESS
SEARCH
Search
Advanced Search
mobile menu button

Articles

Page Path
HOME > J. Microbiol > Volume 55(11); 2017 > Article
Journal Article
Dense Granule Protein-7 (GRA-7) of Toxoplasma gondii inhibits viral replication in vitro and in vivo
Prasanna Weeratunga 1, Thilina U. B. Herath 1, Tae-Hwan Kim 1, Hyun-Cheol Lee 1, Jae-Hoon Kim 1, Byeong-Hoon Lee 1, Eun-Seo Lee 1, Kiramage Chathuranga 1, W. A. Gayan Chathuranga 1, Chul-Su Yang 2, Jin Yeul Ma 3, Jong-Soo Lee 1
Journal of Microbiology 2017;55(11):909-917.
DOI: https://doi.org/10.1007/s12275-017-7392-5
Published online: October 27, 2017
1College of Veterinary Medicine, Chungnam National University, Daejeon 34134, Republic of Korea, 2Department of Molecular and Life Science, College of Science and Technology, Hanyang University, Ansan 15588, Republic of Korea, 3Korean Medicine (KM) Application Center, Korea Institute of Oriental Medicine, Daegu 41062, Republic of Korea
Corresponding author:  Jong-Soo Lee , Tel: +82-42-821-6753, 
Received: 14 September 2017   • Revised: 15 October 2017   • Accepted: 16 October 2017
prev
  • 108 Views
  • 0 Download
  • 17 Crossref
  • 17 Scopus
Full Article

Abstract

Dense granule protein-7 (GRA-7) is an excretory protein of Toxoplasma gondii. It is a potential serodiagnostic marker and vaccine candidate for toxoplasmosis. Previous reports demonstrated that GRA-7 induces innate immune responses in macrophages by interacting with TRAF6 via the MyD88- dependent pathway. In the present study, we evaluated the antiviral activity and induction of an antiviral state by GRA-7 both in vitro and in vivo. It was observed that GRA-7 markedly reduced the replication of vesicular stomatitis virus (VSVGFP), influenza A virus (PR8-GFP), coxsackievirus (H3- GFP), herpes simplex virus (HSV-GFP), and adenovirus-GFP in epithelial (HEK293T/HeLa) and immune (RAW264.7) cells. These antiviral activities of GRA-7 were attributed to the induction of type I interferon (IFN) signaling, resulting in the secretion of IFNs and pro-inflammatory cytokines. Additionally, in BALB/c mice, intranasal administration of GRA-7 prevented lethal infection by influenza A virus (H1N1) and exhibited prophylactic effects against respiratory syncytial virus (RSV-GFP). Collectively, these results suggested that GRA-7 exhibits immunostimulatory and broad spectrum antiviral activities via type I IFN signaling. Thus, GRA-7 can be potentially used as a vaccine adjuvant or as a candidate drug with prophylactic potential against viruses.

  • Cite this Article
    Cite this Article
    export Copy Download
    Close
    Download Citation
    Download a citation file in RIS format that can be imported by all major citation management software, including EndNote, ProCite, RefWorks, and Reference Manager.
    Format:
    • RIS — For EndNote, ProCite, RefWorks, and most other reference management software
    • BibTeX — For JabRef, BibDesk, and other BibTeX-specific software
    Include:
    • Citation for the content below
    Dense Granule Protein-7 (GRA-7) of Toxoplasma gondii inhibits viral replication in vitro and in vivo
    J. Microbiol. 2017;55(11):909-917.   Published online October 27, 2017
    DOI: https://doi.org/10.1007/s12275-017-7392-5
    Close
Related articles
Journal of Microbiology : Journal of Microbiology
© The Microbiological Society of Korea.
TOP