- Volume 50(2); April 2012
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Research Support, Non-U.S. Gov'ts
- TBC: A Clustering Algorithm Based on Prokaryotic Taxonomy
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Jae-Hak Lee , Hana Yi , Yoon-Seong Jeon , Sungho Won , Jongsik Chun
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J. Microbiol. 2012;50(2):181-185. Published online April 27, 2012
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DOI: https://doi.org/10.1007/s12275-012-1214-6
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268
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Abstract
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High-throughput DNA sequencing technologies have revolutionized
the study of microbial ecology. Massive sequencing
of PCR amplicons of the 16S rRNA gene has been
widely used to understand the microbial community structure
of a variety of environmental samples. The resulting
sequencing reads are clustered into operational taxonomic
units that are then used to calculate various statistical indices
that represent the degree of species diversity in a given
sample. Several algorithms have been developed to perform
this task, but they tend to produce different outcomes.
Herein, we propose a novel sequence clustering algorithm,
namely Taxonomy-Based Clustering (TBC). This algorithm
incorporates the basic concept of prokaryotic taxonomy in
which only comparisons to the type strain are made and used
to form species while omitting full-scale multiple sequence
alignment. The clustering quality of the proposed method was
compared with those of MOTHUR, BLASTClust, ESPRITTree,
CD-HIT, and UCLUST. A comprehensive comparison
using three different experimental datasets produced by
pyrosequencing demonstrated that the clustering obtained
using TBC is comparable to those obtained using MOTHUR
and ESPRIT-Tree and is computationally efficient. The program
was written in JAVA and is available from http://sw.
ezbiocloud.net/tbc.
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- Application of Machine Learning to Solid Particle Erosion of APS-TBC and EB-PVD TBC at Elevated Temperatures
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Coatings.2021; 11(7): 845. CrossRef - Effects of Disease Resistant Genetically Modified Rice on Soil Microbial Community Structure According to Growth Stage
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Korean Journal of Environmental Agriculture.2019; 38(3): 185. CrossRef - Effects of the Brown Seaweed Laminaria japonica Supplementation on Serum Concentrations of IgG, Triglycerides, and Cholesterol, and Intestinal Microbiota Composition in Rats
Jae-Young Kim, Young Min Kwon, In-Sung Kim, Jeong-A. Kim, Da-Yoon Yu, Bishnu Adhikari, Sang-Suk Lee, In-Soon Choi, Kwang-Keun Cho
Frontiers in Nutrition.2018;[Epub] CrossRef - hc-OTU: A Fast and Accurate Method for Clustering Operational Taxonomic Units Based on Homopolymer Compaction
Seunghyun Park, Hyun-soo Choi, Byunghan Lee, Jongsik Chun, Joong-Ho Won, Sungroh Yoon
IEEE/ACM Transactions on Computational Biology and Bioinformatics.2018; 15(2): 441. CrossRef - Cloacal Microbiome Structure in a Long-Distance Migratory Bird Assessed Using Deep 16sRNA Pyrosequencing
Jakub Kreisinger, Dagmar Čížková, Lucie Kropáčková, Tomáš Albrecht, Roberto Ambrosini
PLOS ONE.2015; 10(9): e0137401. CrossRef - Metagenomic Insights into the Bioaerosols in the Indoor and Outdoor Environments of Childcare Facilities
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PLOS ONE.2015; 10(5): e0126960. CrossRef - Effect of genetically modified rice producing resveratrol on the soil microbial communities
Soo-In Sohn, Young-Ju Oh, Byung-Yong Kim, Soon-Jong Kweon, Hyun-Suk Cho, Tae-Hoon Ryu
Journal of the Korean Society for Applied Biological Chemistry.2015; 58(6): 795. CrossRef - Algal and Fungal Diversity in Antarctic Lichens
Chae Haeng Park, Kyung Mo Kim, Arve Elvebakk, Ok‐Sun Kim, Gajin Jeong, Soon Gyu Hong
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Environmental Microbiology.2014; 16(6): 1566. CrossRef - Bacterial community of sediments from the Australian-Antarctic ridge
Yung Mi Lee, Doshik Hahm, You-Jung Jung, Sung Hyun Park, Jongsik Chun, Soon Gyu Hong
Polar Biology.2014; 37(4): 587. CrossRef - Performance and bacterial communities of successive alkalinity-producing systems (SAPSs) in passive treatment processes treating mine drainages differing in acidity and metal levels
Sokhee Philemon Jung, Youngwook Cheong, Giljae Yim, Sangwoo Ji, Hojeong Kang
Environmental Science and Pollution Research.2014; 21(5): 3722. CrossRef - Unraveling the outcome of 16S rDNA-based taxonomy analysis through mock data and simulations
Ali May, Sanne Abeln, Wim Crielaard, Jaap Heringa, Bernd W. Brandt
Bioinformatics.2014; 30(11): 1530. CrossRef - Clinical Microbiology Informatics
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Mira Park, Hyoungseok Lee, Soon Gyu Hong, Ok-Sun Kim
Antarctic Science.2013; 25(1): 51. CrossRef - A Unique Prokaryotic Assemblage of Wall Biofilm of a Volcanic Cave (Daesubee) in Jeju
Jong-Geun Moon, Man-Young Jung, Jong-Geol Kim, Soo-Je Park, Dae-Shin Kim, Jong-Shik Kim, Sung-Keun Rhee
The Korean Journal of Microbiology.2013; 49(2): 184. CrossRef - Resolving Ambiguity of Species Limits and Concatenation in Multilocus Sequence Data for the Construction of Phylogenetic Supermatrices
Douglas Chesters, Alfried P. Vogler
Systematic Biology.2013; 62(3): 456. CrossRef - CLUSTOM: A Novel Method for Clustering 16S rRNA Next Generation Sequences by Overlap Minimization
Kyuin Hwang, Jeongsu Oh, Tae-Kyung Kim, Byung Kwon Kim, Dong Su Yu, Bo Kyeng Hou, Gustavo Caetano-Anollés, Soon Gyu Hong, Kyung Mo Kim, George E. Fox
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Mincheol Kim, Ki-Hyun Lee, Seok-Whan Yoon, Bong-Soo Kim, Jongsik Chun, Hana Yi
Genomics & Informatics.2013; 11(3): 102. CrossRef - Bacterial Diversity in the Guts of Sea Cucumbers (Apostichopus japonicus) and Shrimps (Litopenaeus vannamei) Investigated with Tag-Encoded 454 Pyrosequencing of 16S rRNA Genes
Eun Soo Noh, Young-Sam Kim, Dong-Hyun Kim, Kyoung-Ho Kim
The Korean Journal of Microbiology.2013; 49(3): 237. CrossRef - Bacterial diversity in ornithogenic soils compared to mineral soils on King George Island, Antarctica
Ok-Sun Kim, Namyi Chae, Hyun Soo Lim, Ahnna Cho, Jeong Hoon Kim, Soon Gyu Hong, Jeongsu Oh
Journal of Microbiology.2012; 50(6): 1081. CrossRef
- Identification and Enumeration of Microcystis Using a Sandwich Hybridization Assay
-
Jing Ping Zhu , Xian Li , Shi Du
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J. Microbiol. 2012;50(2):186-190. Published online April 27, 2012
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DOI: https://doi.org/10.1007/s12275-012-1418-9
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249
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3
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Abstract
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Based on sequence analyses of phycocyanin intergenic spacers
(PC-IGS) from Microcystis, Anabaena, Aphanizomenon,
and Planktothrix (Oscillatoria) strains, a genus-specific probe
pair TF/TR was designed, and a sandwich hybridization assay
was established to quantitatively detect Microcystis. Through
BLAST and cyanobacterial culture tests, TF/TR was demonstrated
to be specific for Microcystis. A calibration curve
for the sandwich hybridization assay was established, and
the lowest detected concentration was 100 cell/ml. Laboratory
and field samples were analyzed with both sandwich hybridization
assay and microscopy. The biotic and abiotic
components of the samples were of little disturbance to the
sandwich hybridization assay. The results showed no distinct
difference between the two methods. In this study, a sandwich
hybridization assay was established to detect Microcystis,
providing an alternative to traditional microscopic, morphology-
based methods.
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- The Need to Increase Strain-Specific DNA Information from the Invasive Cyanobacteria Sphaerospermopsis aphanizomenoides and Cuspidothrix issatschenkoi
Daniela R. de Figueiredo
Water.2025; 17(4): 579. CrossRef - Molecular techniques for understanding harmful algal blooms: A review
Jackson Sanders, Senjie Lin
Harmful Algae.2025; 148: 102909. CrossRef - Sandwich Hybridization Assay for In Situ Real-Time Cyanobacterial Detection and Monitoring: A Review
Ping Gong, Anna K. Antrim, Sarah R. Bickman, Emily G. Cooley, Seung Ho Chung
Biosensors.2022; 12(8): 640. CrossRef
- Isolation and Characterization of Plant Growth-Promoting Rhizobacteria from Wheat Roots by Wheat Germ Agglutinin Labeled with Fluorescein Isothiocyanate
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Jian Zhang , Jingyang Liu , Liyuan Meng , Zhongyou Ma , Xinyun Tang , Yuanyuan Cao , Leni Sun
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J. Microbiol. 2012;50(2):191-198. Published online April 27, 2012
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DOI: https://doi.org/10.1007/s12275-012-1472-3
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315
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35
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Thirty-two isolates were obtained from wheat rhizosphere
by wheat germ agglutinin (WGA) labeled with fluorescein
isothiocyanate (FITC). Most isolates were able to produce
indole acetic acid (65.6%) and siderophores (59.3%), as well
as exhibited phosphate solubilization (96.8%). Fourteen isolates
displayed three plant growth-promoting traits. Among
these strains, two phosphate-dissolving ones, WS29 and
WS31, were evaluated for their beneficial effects on the early
growth of wheat (Triticum aestivum Wan33). Strain WS29
and WS31 significantly promoted the development of lateral
roots by 34.9% and 27.6%, as well as increased the root dry
weight by 25.0% and 25.6%, respectively, compared to those
of the control. Based on 16S rRNA gene sequence comparisons
and phylogenetic positions, both isolates were determined
to belong to the genus Bacillus. The proportion of
isolates showing the properties of plant growth-promoting
rhizobacteria (PGPR) was higher than in previous reports.
The efficiency of the isolation of PGPR strains was also
greatly increased by WGA labeled with FITC. The present
study indicated that WGA could be used as an effective tool
for isolating PGPR strains with high affinity to host plants
from wheat roots. The proposed approach could facilitate
research on biofertilizers or biocontrol agents.
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Journal Article
- Detecting Nonculturable Bacteria in the Active Mycorrhizal Zone of the Pine Mushroom Tricholoma matsutake
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Ryota Kataoka , Zaki Anwar Siddiqui , Junichi Kikuchi , Masaki Ando , Rina Sriwati , Ai Nozaki , Kazuyoshi Futai
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J. Microbiol. 2012;50(2):199-206. Published online April 27, 2012
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DOI: https://doi.org/10.1007/s12275-012-1371-7
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283
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Abstract
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The fungus Tricholoma matsutake forms an ectomycorrhizal
relationship with pine trees. Its sporocarps often develop in a
circle, which is commonly known as a fairy ring. The fungus
produces a solid, compact, white aggregate of mycelia and
mycorrhizae beneath the fairy ring, which in Japanese is
called a ‘shiro’. In the present study, we used soil dilution
plating and molecular techniques to analyze the bacterial
communities within, beneath, and outside the T. matsutake
fairy ring. Soil dilution plating confirmed previous reports
that bacteria and actinomycetes are seldom present in the
soil of the active mycorrhizal zone of the T. matsutake shiro.
In addition, the results showed that the absence of bacteria
was strongly correlated with the presence of T. matsutake
mycorrhizae. The results demonstrate that bacteria, especially
aerobic and heterotrophic forms, and actinomycetes,
are strongly inhibited by T. matsutake. Indeed, neither bacteria
nor actinomycetes were detected in 11.3% of 213 soil
samples from the entire shiro area by culture-dependent
methods
. However, molecular techniques demonstrated that
some bacteria, such as individual genera of Sphingomonas
and Acidobacterium, were present in the active mycorrhizal
zone, even though they were not detected in soil assays using
the dilution plating technique.
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Research Support, Non-U.S. Gov'ts
- Molecular Analysis of Spatial Variation of Iron-Reducing Bacteria in Riverine Alluvial Aquifers of the Mankyeong River
-
So-Jeong Kim , Dong-Chan Koh , Soo-Je Park , In-Tae Cha , Joong-Wook Park , Jong-Hwa Na , Yul Roh , Kyung-Seok Ko , Kangjoo Kim , Sung-Keun Rhee
-
J. Microbiol. 2012;50(2):207-217. Published online April 27, 2012
-
DOI: https://doi.org/10.1007/s12275-012-1342-z
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242
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Abstract
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Alluvial aquifers are one of the mainwater resources in
many countries. Iron reduction in alluvial aquifers is often
a major anaerobic process involved in bioremediation or
causing problems, including the release of As trapped in
Fe(III) oxide. We investigated the distribution of potential
iron-reducing bacteria (IRB) in riverine alluvial aquifers
(B1, B3, and B6 sites) at the Mankyeong River, Republic of
Korea. Inactive iron reduction zones, the diversity and abundance
of IRB can be examined using a clone library and
quantitative PCR analysis of 16S rRNA genes. Geobacter
spp. are potential IRB in the iron-reducing zone at the B6
(9 m) site, where high Fe(II) and arsenic (As) concentrations
were observed. At the B3 (16 m) site, where low iron reduction
activity was predicted, a dominant clone (10.6%) was
99% identical in 16S rRNA gene sequence with Rhodoferax
ferrireducens. Although a major clone belonging to Clostridium
spp. was found, possible IRB candidates could not be
unambiguously determined at the B1 (18 m) site. Acanonical
correspondence analysis demonstrated that, among potential
IRB, only the Geobacteraceae were well correlated with Fe(II)
and As concentrations. Our results indicate high environmental
heterogeneity, and thus high spatial variability, in
thedistribution of potential IRB in the riverine alluvial
aquifersnear the Mankyeong River.
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- Microbial Fingerprinting Detects Unique Bacterial Communities in the Faecal Microbiota of Rats with Experimentally-Induced Colitis
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Ashis K. Samanta , Valeria A. Torok , Nigel J. Percy , Suzanne M. Abimosleh , Gordon S. Howarth
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J. Microbiol. 2012;50(2):218-225. Published online April 27, 2012
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DOI: https://doi.org/10.1007/s12275-012-1362-8
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251
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39
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Abstract
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An abnormal composition of the gut microbiota is believed
to be associated with the pathogenesis of inflammatory
bowel disease (IBD). We utilized terminal restriction fragment
length polymorphism (T-RFLP) analysis to quantify
faecal bacterial communities from rats with experimental
colitis. Male Sprague Dawley rats (n=10/group) ingested
2% dextran sulfate sodium (DSS) or water for up to 7 days.
Rats were killed and colonic tissues collected for histological
analysis. Damage severity score in the distal colon was significantly
greater (P<0.001) following DSS consumption
compared to controls. T-RFLP faecal bacterial profiles generated
with either MspI or CfoI revealed a significant difference
(P<0.001) in community composition between healthy
and colitic rats, with bacterial composition in healthy rats
more variable than in rats with colitis. Operational taxonomic
units (OTU: taxonomically related groups of bacteria)
associated with either the healthy or colitic state were
identified. OTU (116, 226, 360, and 948; CfoI) and (118 and
188; MspI) were strongly associated with untreated healthy
rats, while OTU (94, 98, 174, and 384; CfoI) and (94 and
914; MspI) were predominantly associated with DSS-treated
colitic rats. Phylogenetic OTU assignment suggested that
Bacteroidales and Lactobacillus sp. were predominantly associated
with the colitic and healthy rats, respectively. These
results
show that faecal bacterial profiling is a rapid, sensitive
and non-invasive tool for detecting and identifying changes
in gut microbiota associated with colitis. Restoring microbial
homeostasis by targeting colitis-associated OTU through
specific microbiological interventions could form the basis
of novel therapeutic strategies for IBD.
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- Effect of Natural Mediators on the Stability of Trametes trogii Laccase during the Decolourization of Textile Wastewaters
-
Rim Khlifi-Slama , Tahar Mechichi , Sami Sayadi , Abdelhafidh Dhouib
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J. Microbiol. 2012;50(2):226-234. Published online April 27, 2012
-
DOI: https://doi.org/10.1007/s12275-012-1421-1
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219
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Abstract
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The purpose of the present study was to determine the
effect of natural mediators on the stability of the Trametes
trogii crude laccase in the process of decolourization of
textile effluents. Acetosyringone allowed the highest wastewaters
decolourization rate of 25%. At higher concentrations
of acetosyringone, the relative activity of laccase decreased
approximately by between 38% and 88% after 5 days of
incubation. T. trogii laccase was strongly inactivated at 3
mM syringaldehyde, after 3 days of incubation. However,
laccase activity is more stable in the presence of the vanillin
and m-coumarate. The T. trogii growth on solid effluentbased-
medium was examined and evaluated by measuring
the colony diameter in cm. T. trogii was completely inhibited
on 100:0 and 80:20 effluent:water solid medium, however,
colony diameter reached 5 cm on 60:40 effluent:water solid
medium after 13–14 days incubation. When the textile effluent
was pre-treated with laccase and laccase-acetosyringone
system, the colony diameter of 2 cm of T. trogii on 80:20
effluent:water solid medium was reached after 14 and 10
days of incubation respectively.
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Sunena Jassal, Rahul Warmoota, Anupama Sharma, Sarita Sheoran, Deepak Kumar, Naveen Gupta
Indian Journal of Microbiology.2024;[Epub] CrossRef - Fabrication, characterization, and application of laccase-immobilized membranes for acetamiprid and diuron degradation
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International Journal of Biological Macromolecules.2024; 282: 136787. CrossRef - Mechanism of norfloxacin transformation by horseradish peroxidase and various redox mediated by humic acid and microplastics
Yifei Leng, Feiyu Liu, Huiping Cai, Fengyi Chang, Wen Xiong, Shushi Huang, Jun Wang
Science of The Total Environment.2023; 858: 159528. CrossRef - Hybrid electrocoagulation and laccase mediated treatment for efficient decolorization of effluent generated from textile industries
Shweta Kalia, Vivek Dalvi, Vivek Kumar Nair, Saurabh Samuchiwal, Anushree Malik
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Nigella Mendes De Paula, Krisle da Silva, Tatiane Brugnari, Charles Windson Isidoro Haminiuk, Giselle Maria Maciel
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Mira chares Subash, A.S. Maheshwari
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Yaohua Gu, Lin Yuan, Leina Jia, Ping Xue, Huiqin Yao
RSC Advances.2021; 11(47): 29498. CrossRef - Removal of trace organic contaminants by enzymatic membrane bioreactors: Role of membrane retention and biodegradation
Muhammad Bilal Asif, Jingwei Hou, William E. Price, Vicki Chen, Faisal I. Hai
Journal of Membrane Science.2020; 611: 118345. CrossRef - Rapid catalytic oxidation of As(iii) to As(v) using aBacillusspore–2,2,6,6-tetramethylpiperidine-1-oxyl system
Yuqing Qin, Fei Peng, Yonggang Hu
Green Chemistry.2019; 21(9): 2286. CrossRef - Optimization of a laccase-mediator system with natural redox-mediating compounds for pesticide removal
Larine Kupski, Gabriela M. Salcedo, Sergiane S. Caldas, Taiana D. de Souza, Eliana B. Furlong, Ednei G. Primel
Environmental Science and Pollution Research.2019; 26(5): 5131. CrossRef - The development of CotA mediator cocktail system for dyes decolorization
S. Luo, T. Xie, Z. Liu, F. Sun, G. Wang
Journal of Applied Microbiology.2018; 124(5): 1164. CrossRef - Linking Enzymatic Oxidative Degradation of Lignin to Organics Detoxification
Xiaolu Wang, Bin Yao, Xiaoyun Su
International Journal of Molecular Sciences.2018; 19(11): 3373. CrossRef - Simultaneous production of laccase and degradation of bisphenol A with Trametes versicolor cultivated on agricultural wastes
Shengquan Zeng, Jie Zhao, Liming Xia
Bioprocess and Biosystems Engineering.2017; 40(8): 1237. CrossRef - Degradation of Trace Organic Contaminants by a Membrane Distillation—Enzymatic Bioreactor
Muhammad Asif, Faisal Hai, Jinguo Kang, Jason Van de Merwe, Frederic Leusch, Kazuo Yamamoto, William Price, Long Nghiem
Applied Sciences.2017; 7(9): 879. CrossRef - Degradation of the herbicide isoproturon by laccase-mediator systems
Shengquan Zeng, Xueli Qin, Liming Xia
Biochemical Engineering Journal.2017; 119: 92. CrossRef - Degradation of Dyes by Laccase
Alper Akkaya, Emine Erdogan Ozseker, Hatice Ardag Akdogan
Analytical Letters.2016; 49(6): 790. CrossRef - Spectroscopic and computational characterization of laccases and their substrate radical intermediates
Rebecca Pogni, Maria Camilla Baratto, Adalgisa Sinicropi, Riccardo Basosi
Cellular and Molecular Life Sciences.2015; 72(5): 885. CrossRef - Optimization of Anthraquinone Dyes Decolorization Conditions with Response Surface Methodology by Aspergillus
Yufeng Ge, Bin Wei, Siyu Wang, Zhiguo Guo, Xiaolin Xu
Korean Chemical Engineering Research.2015; 53(3): 327. CrossRef - The effects of mediator and granular activated carbon addition on degradation of trace organic contaminants by an enzymatic membrane reactor
Luong N. Nguyen, Faisal I. Hai, William E. Price, Frederic D.L. Leusch, Felicity Roddick, Hao H. Ngo, Wenshan Guo, Saleh F. Magram, Long D. Nghiem
Bioresource Technology.2014; 167: 169. CrossRef - An enzymatic approach to develop a lignin-based adhesive for wool floor coverings
E. Aracri, C. Díaz Blanco, T. Tzanov
Green Chemistry.2014; 16(5): 2597. CrossRef - Enhancement of trace organic contaminant degradation by crude enzyme extract from Trametes versicolor culture: Effect of mediator type and concentration
Luong N. Nguyen, Faisal I. Hai, Jinguo Kang, Frederic D.L. Leusch, Felicity Roddick, Saleh F. Magram, William E. Price, Long D. Nghiem
Journal of the Taiwan Institute of Chemical Engineers.2014; 45(4): 1855. CrossRef - A spectroscopic characterization of a phenolic natural mediator in the laccase biocatalytic reaction
Andrea Martorana, Lorenzo Sorace, Harry Boer, Rafael Vazquez-Duhalt, Riccardo Basosi, Maria Camilla Baratto
Journal of Molecular Catalysis B: Enzymatic.2013; 97: 203. CrossRef
- Purification and Structure Analysis of Mycolic Acids in Corynebacterium glutamicum
-
Yang Yang , Feng Shi , Guanjun Tao , Xiaoyuan Wang
-
J. Microbiol. 2012;50(2):235-240. Published online April 27, 2012
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DOI: https://doi.org/10.1007/s12275-012-1459-0
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Abstract
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Corynebacterium glutamicum is widely used for producing
amino acids. Mycolic acids, the major components in the cell
wall of C. glutamicum might be closely related to the secretion
of amino acids. In this study, mycolic acids were extracted
from 5 strains of C. glutamicum, including ATCC 13032,
ATCC 13869, ATCC 14067, L-isoleucine producing strain
IWJ-1, and L-valine producing strain VWJ-1. Structures of
these mycolic acids were analyzed using thin layer chromatography
and electrospray ionization mass spectrometry.
More than twenty molecular species of mycolic acid were
observed in all 5 strains. They differ in the length (20–40
carbons) and saturation (0–3 double bonds) of their constituent
fatty acids. The dominant species of mycolic acid in
every strain was different, but their two hydrocarbon chains
were similar in length (14–18 carbons), and the meromycolate
chain usually contained double bonds. As the growth
temperature of cells increased from 30°C to 34°C, the proportion
of mycolic acid species containing unsaturated and
shorter hydrocarbon chains increased. These results provide
new information on mycolic acids in C. glutamicum,
and could be useful for modifying the cell wall to increase
the production of amino acids.
-
Citations
Citations to this article as recorded by

- Metabolic engineering of Corynebacterium glutamicum for L-alanine production
Yu Huang, Hedan Li, Guihong Zhao, Xiaoqing Hu, Xiaoyuan Wang
Systems Microbiology and Biomanufacturing.2025; 5(1): 261. CrossRef - Enhanced biodesulfurization of recalcitrant organosulfur compounds by a newly identified Gordonia alkanivorans JT-2 in the presence of mycolic acid
Maosheng Yin, Shuiquan Chen, Meng Zang, Lin Li, Chaocheng Zhao, Xiuxia Zhang
Biochemical Engineering Journal.2025; 221: 109779. CrossRef - The role of trehalose biosynthesis on mycolate composition and L-glutamate production in Corynebacterium glutamicum
Hedan Li, Daqing Xu, Xin Tan, Danyang Huang, Yu Huang, Guihong Zhao, Xiaoqing Hu, Xiaoyuan Wang
Microbiological Research.2023; 267: 127260. CrossRef - Advances in Synthetic Biology Techniques and Industrial Applications of Corynebacterium glutamicum
Yujue Wang, Qiang Wang, Aobo Sha, Kexin Ren, Mengkai Hu, Meijuan Xu, Xian Zhang, Zhiming Rao
Fermentation.2023; 9(8): 729. CrossRef - From the banal to the bizarre: unravelling immune recognition and response to microbial lipids
Laura Burchill, Spencer J. Williams
Chemical Communications.2022; 58(7): 925. CrossRef - Preventing mycolic acid reduction in Corynebacterium glutamicum can efficiently increase L-glutamate production
Hedan Li, Daqing Xu, Yuantao Liu, Xin Tan, Jun Qiao, Zihan Li, Bin Qi, Xiaoqing Hu, Xiaoyuan Wang
Biochemical Engineering Journal.2022; 177: 108255. CrossRef - Effect of Thermophilic Microbial Agents on Antibiotic Resistance Genes and Microbial Communities during Co-Composting of Pig Manure and Tea Stalks
Xiaojia Chen, Chengjian Wu, Qinyu Li, Peng Zhou, Zheng Chen, Yang Han, Jiping Shi, Zhijun Zhao
Sustainability.2022; 14(19): 12593. CrossRef - Genome Sequence of the Bacteriophage CL31 and Interaction with the Host Strain Corynebacterium glutamicum ATCC 13032
Max Hünnefeld, Ulrike Viets, Vikas Sharma, Astrid Wirtz, Aël Hardy, Julia Frunzke
Viruses.2021; 13(3): 495. CrossRef - Efficient biodesulfurization of diesel oil by Gordonia sp. SC-10 with highly hydrophobic cell surfaces
Shuiquan Chen, Meng Zang, Lin Li, Juntao Chen, Qiyou Liu, Xiwen Feng, Shuo Sun, Chuanwei Zang, Chaocheng Zhao
Biochemical Engineering Journal.2021; 174: 108094. CrossRef - Lack of MSMEG_6281, a peptidoglycan amidase, affects cell wall integrity and virulence of Mycobacterium smegmatis
Jiajia He, Weizhe Fu, Shijia Zhao, Cuili Zhang, Tieying Sun, Tao Jiang
Microbial Pathogenesis.2019; 128: 405. CrossRef - Study of the conformational behaviour of trehalose mycolates by FT-IR spectroscopy
F. Migliardo, Y. Bourdreux, M. Buchotte, G. Doisneau, J.-M. Beau, N. Bayan
Chemistry and Physics of Lipids.2019; 223: 104789. CrossRef - Identification of novel lipid modifications and intermembrane dynamics in Corynebacterium glutamicum using high-resolution mass spectrometry [S]
Stephan Klatt, Rajini Brammananth, Sean O'Callaghan, Konstantinos A. Kouremenos, Dedreia Tull, Paul K. Crellin, Ross L. Coppel, Malcolm J. McConville
Journal of Lipid Research.2018; 59(7): 1190. CrossRef - Impact of mycolic acid deficiency on cells of Corynebacterium glutamicum ATCC13869
Yunfei Gao, Xiaoqing Hu, Jianli Wang, Huazhong Li, Xiaoyuan Wang
Biotechnology and Applied Biochemistry.2018; 65(3): 435. CrossRef - The role of corynomycolic acids in Corynebacterium-host interaction
Andreas Burkovski
Antonie van Leeuwenhoek.2018; 111(5): 717. CrossRef - CORYNEBACTERIUM: FEATURES OF THE STRUCTURE OF THE BACTERIAL CELL
G. G. Kharseeva, N. A. Voronina
Journal of microbiology, epidemiology and immunobiology.2017; 94(1): 107. CrossRef - Sensing Lipids with Mincle: Structure and Function
Spencer J. Williams
Frontiers in Immunology.2017;[Epub] CrossRef - Protein secretion inCorynebacterium glutamicum
Xiuxia Liu, Wei Zhang, Zihao Zhao, Xiaofeng Dai, Yankun Yang, Zhonghu Bai
Critical Reviews in Biotechnology.2017; 37(4): 541. CrossRef - Visualization of mycobacterial membrane dynamics in live cells
Frances P. Rodriguez-Rivera, Xiaoxue Zhou, Julie A. Theriot, Carolyn R. Bertozzi
Journal of the American Chemical Society.2017; 139(9): 3488. CrossRef - Lipid structure influences the ability of glucose monocorynomycolate to signal through Mincle
Phillip L. van der Peet, Masahiro Nagata, Sayali Shah, Jonathan M. White, Sho Yamasaki, Spencer J. Williams
Organic & Biomolecular Chemistry.2016; 14(39): 9267. CrossRef - Lipid metabolism in mycobacteria—Insights using mass spectrometry-based lipidomics
Peter J. Crick, Xue Li Guan
Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids.2016; 1861(1): 60. CrossRef - Corynomycolic acid-containing glycolipids signal through the pattern recognition receptor Mincle
Phillip L. van der Peet, Christian Gunawan, Shota Torigoe, Sho Yamasaki, Spencer J. Williams
Chemical Communications.2015; 51(24): 5100. CrossRef - Acetylation of Trehalose Mycolates Is Required for Efficient MmpL-Mediated Membrane Transport in Corynebacterineae
Yoshiki Yamaryo-Botte, Arek K. Rainczuk, David J. Lea-Smith, Rajini Brammananth, Phillip L. van der Peet, Peter Meikle, Julie E. Ralton, Thusita W. T. Rupasinghe, Spencer J. Williams, Ross L. Coppel, Paul K. Crellin, Malcolm J. McConville
ACS Chemical Biology.2015; 10(3): 734. CrossRef - Current knowledge on mycolic acids in Corynebacterium glutamicum and their relevance for biotechnological processes
Marie-Antoinette Lanéelle, Maryelle Tropis, Mamadou Daffé
Applied Microbiology and Biotechnology.2013; 97(23): 9923. CrossRef - Cell Envelope of Corynebacteria: Structure and Influence on Pathogenicity
Andreas Burkovski
ISRN Microbiology.2013; 2013: 1. CrossRef
- Effects of Exopolysaccharide Production on Liquid Vegetative Growth, Stress Survival, and Stationary Phase Recovery in Myxococcus xanthus
-
Wei Hu , Jing Wang , Ian McHardy , Renate Lux , Zhe Yang , Yuezhong Li , Wenyuan Shi
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J. Microbiol. 2012;50(2):241-248. Published online April 27, 2012
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DOI: https://doi.org/10.1007/s12275-012-1349-5
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178
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11
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Abstract
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Exopolysaccharide (EPS) of Myxococcus xanthus is a wellregulated
cell surface component. In addition to its known
functions for social motility and fruiting body formation
on solid surfaces, EPS has also been proposed to play a role
in multi-cellular clumping in liquid medium, though this
phenomenon has not been well studied. In this report, we
confirmed that M. xanthus clumps formed in liquid were
correlated with EPS levels and demonstrated that the EPS
encased cell clumps exhibited biofilm-like structures. The
clumps protected the cells at physiologically relevant EPS
concentrations, while cells lacking EPS exhibited significant
reduction in long-term viability and resistance to stressful
conditions. However, excess EPS production was counterproductive
to vegetative growth and viable cell recovery declined
in extended late stationary phase as cells became
trapped in the matrix of clumps. Therefore, optimal EPS
production by M. xanthus is important for normal physiological
functions in liquid.
- Development of a Suicidal Vector-Cloning System Based on Butanal Susceptibility Due to an Expression of YqhD Aldehyde Reductase
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Changhan Lee , Chankyu Park
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J. Microbiol. 2012;50(2):249-255. Published online April 27, 2012
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DOI: https://doi.org/10.1007/s12275-012-1438-5
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178
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4
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Abstract
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Previously, we observed butanal/propanal sensitivity of
Escherichia coli K-12 when cells overexpress YqhD protein,
a NADPH dependent aldehyde reductase, possibly due to
an accumulation of butanol/propanol in vivo as the reaction
products. Based on this finding, we developed a suicidal
vector-cloning system derived from pUC19, in which lacZ
was substituted with the yqhD gene. As a result, when foreign
DNA was inserted into its multiple cloning sites by disrupting
an expression of YqhD, the recombinants survived on
butanal/propanal containing plate, whereas cells containing
the YqhD vector died because of the alcohol production
by YqhD. The cloning efficiency, estimated based on colony
PCR and enzyme digestion, was achieved more than 90%
when the suicidal vector system was used. Moreover, the
plasmid vector itself was stably maintained in the cell, presumably
due to its ability to remove toxic aldehydes being
accumulated in E. coli cell by metabolic stress.
- Effects of Mutations in the WD40 Domain of α-COP on Its Interaction with the COPI Coatomer in Saccharomyces cerevisiae
-
Ki-Hyun Kim , Eun Kyung Kim , Ki Young Jeong , Yun-Hee Park , Hee-Moon Park
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J. Microbiol. 2012;50(2):256-262. Published online April 27, 2012
-
DOI: https://doi.org/10.1007/s12275-012-1326-z
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247
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5
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Abstract
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Replacement of glycine 227 in the fifth WD40 motif of
α-COP/Ret1p/Soo1p by charged or aromatic amino acids is
responsible for the temperature-dependent osmo-sensitivity
of Saccharomyces cerevisiae, while truncations of WD40
motifs exerted a reduction in cell growth rate and impairment
in assembly of cell-wall associated proteins such as
enolase and Gas1p. Yeast two-hybrid analysis revealed that
the ret1-1/soo1-1 mutation of α-COP abolished the interaction
with β- and ε-COP, respectively, and that the interaction
between α-COP and β-COP relied on the WD40 domain
of α-COP. Furthermore, although the WD40 domain
is dispensable for interaction of α-COP with ε-COP, structural
alterations in the WD40 domain could impair the
interaction.
-
Citations
Citations to this article as recorded by

- Dissecting the essential role of N-glycosylation in catalytic performance of xanthan lyase
Jingjing Zhao, Qian Wang, Xin Ni, Shaonian Shen, Chenchen Nan, Xianzhen Li, Xiaoyi Chen, Fan Yang
Bioresources and Bioprocessing.2022;[Epub] CrossRef - Transcriptional Regulation of Anthocyanin Synthesis by MYB-bHLH-WDR Complexes in Kiwifruit (Actinidia chinensis)
Yanfei Liu, Kangxun Ma, Yingwei Qi, Guowen Lv, Xiaolin Ren, Zhande Liu, Fengwang Ma
Journal of Agricultural and Food Chemistry.2021; 69(12): 3677. CrossRef - Identification and expression profile of an alpha-COPI homologous gene (COPA1) involved in high irradiance and salinity stress in Haematococcus pluvialis
Qiulan Luo, Jingjing Ning, Zhangli Hu, Chaogang Wang
Algal Research.2017; 28: 220. CrossRef - Depletion of ε-COP in the COPI Vesicular Coat Reduces Cleistothecium Production inAspergillus nidulans
Eun-Hye Kang, Eun-Jung Song, Jun Ho Kook, Hwan-Hee Lee, Bo-Ri Jeong, Hee-Moon Park
Mycobiology.2015; 43(1): 31. CrossRef - Analysis of Protein Domain for Interaction between α-COP and ε-COP in Aspergillus nidulans
Eun-Jung Song, Ki-Hyun Kim, Hwan-Hee Lee, Jeong-Seok Park, Eun-Hye Kang, Hee-Moon Park
The Korean Journal of Mycology.2012; 40(4): 224. CrossRef
- Morphological Structure of Propagules and Electrophoretic Karyotype Analysis of False Smut Villosiclava virens in Rice
-
Rongtao Fu , Lei Ding , Jun Zhu , Ping Li , Ai-ping Zheng
-
J. Microbiol. 2012;50(2):263-269. Published online April 27, 2012
-
DOI: https://doi.org/10.1007/s12275-012-1456-3
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185
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23
Scopus
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Abstract
PDF
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The target pathogen Villosiclava virens (teleomorph: claviceps
oryzae-sativae) was isolated from the infected rice, where it
caused false smut. In our study, the forming processes of the
chlamydospores, chlamydospore balls, conidiospores, and
secondary conidiospores during the asexual reproduction
were observed more precisely and in greater detail than
previous descriptions. The microstructure of the infected
rice kernel showed that the outer dense chlamydospores
piled around the false smut balls grown on XBZ medium;
moreover the sclerotia consisting of dense mycelium were
found. The different morphology was observed across the
different growing conditions. In addition, we observed the
nuclear numbers of both the conidiospores and hyphae using
4′,6-diamidino-2-phenylindole (DAPI) staining. Because
the fungus has small chromosomes and the numbers were
not previously known, we analyzed the electrophoretic karyotype
using a pulsed field gel electrophoresis (PFGE) technique.
The results showed that V. virens has at least 10 chromosomes
ranging in size from 0.6 kb to 6 Mb. The V. virens
genome size is estimated to be 23 Mb. Here, we report the
morphological characteristics of the fungus and the process
of asexual spores forming asexual propagules, along with
the first analyze the molecular karyotype of V. virens. These
results
supply a foundation for further study of the pathogenicity
and biology of this devastating pathogen.
- Protein-Protein Interactions between Histidine Kinases and Response Regulators of Mycobacterium tuberculosis H37Rv
-
Ha-Na Lee , Kwang-Eun Jung , In-Jeong Ko , Hyung Suk Baik , Jeong-Il Oh
-
J. Microbiol. 2012;50(2):270-277. Published online April 27, 2012
-
DOI: https://doi.org/10.1007/s12275-012-2050-4
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242
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22
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Abstract
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Using yeast two-hybrid assay, we investigated protein-protein
interactions between all orthologous histidine kinase
(HK)/response regulator (RR) pairs of M. tuberculosis H37Rv
and identified potential protein-protein interactions between
a noncognate HK/RR pair, DosT/NarL. The protein
interaction between DosT and NarL was verified by phosphotransfer
reaction from DosT to NarL. Furthermore, we
found that the DosT and DosS HKs, which share considerable
sequence similarities to each other and form a twocomponent
system with the DosR RR, have different crossinteraction
capabilities with NarL: DosT interacted with
NarL, while DosS did not. The dimerization domains of
DosT and DosS were shown to be sufficient to confer specificity
for DosR, and the different cross-interaction abilities
of DosS and DosT with NarL were demonstrated to be attributable
to variations in the amino acid sequences of the
α2-helices of their dimerization domains.
-
Citations
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- Modulators targeting protein-protein interactions in Mycobacterium tuberculosis
Guofeng Luo, Tianqi Ming, Luchuan Yang, Lei He, Tao Tao, Yanmei Wang
Microbiological Research.2024; 284: 127675. CrossRef - Dual functioning by the PhoR sensor is a key determinant to Mycobacterium tuberculosis virulence
Prabhat Ranjan Singh, Harsh Goar, Partha Paul, Khushboo Mehta, Bhanwar Bamniya, Anil Kumar Vijjamarri, Roohi Bansal, Hina Khan, Subramanian Karthikeyan, Dibyendu Sarkar, Danielle A. Garsin
PLOS Genetics.2023; 19(12): e1011070. CrossRef - Phosphoproteomics of Mycobacterium-host interaction and inspirations for novel measures against tuberculosis
Zilu Wang, Jianping Xie
Cellular Signalling.2022; 91: 110238. CrossRef - PrrA modulates Mycobacterium tuberculosis response to multiple environmental cues and is critically regulated by serine/threonine protein kinases
David Giacalone, Rochelle E. Yap, Alwyn M. V. Ecker, Shumin Tan, Danielle A. Garsin
PLOS Genetics.2022; 18(8): e1010331. CrossRef - Sensing of O2 and nitrate by bacteria: alternative strategies for transcriptional regulation of nitrate respiration by O2 and nitrate
Gottfried Unden, Robin Klein
Environmental Microbiology.2021; 23(1): 5. CrossRef - Nitrate-nitrite fate and oxygen sensing in dormant Mycobacterium tuberculosis: A bioinorganic approach highlighting the importance of transition metals
Eduardo H.S. Sousa, Marta S.P. Carepo, José J.G. Moura
Coordination Chemistry Reviews.2020; 423: 213476. CrossRef - Inhibiting Mycobacterium tuberculosis DosRST Signaling by Targeting Response Regulator DNA Binding and Sensor Kinase Heme
Huiqing Zheng, John T. Williams, Bilal Aleiwi, Edmund Ellsworth, Robert B. Abramovitch
ACS Chemical Biology.2020; 15(1): 52. CrossRef - Acetylation of Response Regulator Proteins, TcrX and MtrA in M. tuberculosis Tunes their Phosphotransfer Ability and Modulates Two-Component Signaling Crosstalk
Krishna Kumar Singh, Neerupma Bhardwaj, Gaurav D. Sankhe, Niveda Udaykumar, Rambir Singh, Vandana Malhotra, Deepak Kumar Saini
Journal of Molecular Biology.2019; 431(4): 777. CrossRef - In Vivo Methods to Study Protein–Protein Interactions as Key Players in Mycobacterium Tuberculosis Virulence
Romain Veyron-Churlet, Camille Locht
Pathogens.2019; 8(4): 173. CrossRef - Dual control of RegX3 transcriptional activity by SenX3 and PknB
Eun-Jin Park, Yu-Mi Kwon, Jin-Won Lee, Ho-Young Kang, Jeong-Il Oh
Journal of Biological Chemistry.2019; 294(28): 11023. CrossRef - Oxygen triggers signal transduction in the DevS (DosS) sensor of Mycobacterium tuberculosis by modulating the quaternary structure
Josiane Bezerra da Silva Lobão, Ana C. S. Gondim, Wellinson G. Guimarães, Marie‐Alda Gilles‐Gonzalez, Luiz Gonzaga de França Lopes, Eduardo H. S. Sousa
The FEBS Journal.2019; 286(3): 479. CrossRef - Regulation of Three Virulence Strategies of Mycobacterium tuberculosis: A Success Story
Niels Zondervan, Jesse Van Dam, Peter Schaap, Vitor Martins dos Santos, Maria Suarez-Diez
International Journal of Molecular Sciences.2018; 19(2): 347. CrossRef - Inhibition of the DevSR Two-Component System by Overexpression of Mycobacterium tuberculosis PknB in Mycobacterium smegmatis
Hyun-Jung Bae, Ha-Na Lee, Mi-Na Baek, Eun-Jin Park, Chi-Yong Eom, In-Jeong Ko, Ho-Young Kang, Jeong-Il Oh
Molecules and Cells.2017; 40(9): 632. CrossRef - The two-component signalling networks of Mycobacterium tuberculosis display extensive cross-talk in vitro
Ruchi Agrawal, Akancha Pandey, Mayooreshwar P. Rajankar, Narendra M. Dixit, Deepak K. Saini
Biochemical Journal.2015; 469(1): 121. CrossRef - Phylogenomics of Mycobacterium Nitrate Reductase Operon
Qinqin Huang, Abualgasim Elgaili Abdalla, Jianping Xie
Current Microbiology.2015; 71(1): 121. CrossRef - Genome-wide prediction of prokaryotic two-component system networks using a sequence-based meta-predictor
Altan Kara, Martin Vickers, Martin Swain, David E. Whitworth, Narcis Fernandez-Fuentes
BMC Bioinformatics.2015;[Epub] CrossRef - Two-Component Regulatory Systems of Mycobacteria
Tanya Parish, Graham F. Hatfull, William R. Jacobs Jr.
Microbiology Spectrum.2014;[Epub] CrossRef - Rv1027c–Rv1028c encode functional KdpDE two – Component system in Mycobacterium tuberculosis
Ruchi Agrawal, Deepak Kumar Saini
Biochemical and Biophysical Research Communications.2014; 446(4): 1172. CrossRef - Serine 83 in DosR, a response regulator from Mycobacterium tuberculosis, promotes its transition from an activated, phosphorylated state to an inactive, unphosphorylated state
Ha Yeon Cho, Beom Sik Kang
Biochemical and Biophysical Research Communications.2014; 444(4): 651. CrossRef - Proteome and phosphoproteome analysis of the serine/threonine protein kinase E mutant of Mycobacterium tuberculosis
Dinesh Kumar Parandhaman, Prashant Sharma, Deepa Bisht, Sujatha Narayanan
Life Sciences.2014; 109(2): 116. CrossRef - Improved understanding of pathogenesis from protein interactions inMycobacteriumtuberculosis
Tao Cui, Zheng-Guo He
Expert Review of Proteomics.2014; 11(6): 745. CrossRef - Involvement of the catalytically important Asp54 residue ofMycobacterium smegmatisDevR in protein-protein interactions between DevR and DevS
Ha-Na Lee, Na-On Lee, In-Jeong Ko, Si Wouk Kim, Beom Sik Kang, Jeong-Il Oh
FEMS Microbiology Letters.2013; 343(1): 26. CrossRef
Research Support, U.S. Gov't, Non-P.H.S.
- Shedding of Viral Hemorrhagic Septicemia Virus (Genotype IVb) by Experimentally Infected Muskellunge (Esox masquinongy)
-
Robert K. Kim , Mohamed Faisal
-
J. Microbiol. 2012;50(2):278-284. Published online April 27, 2012
-
DOI: https://doi.org/10.1007/s12275-012-1145-2
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173
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26
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Previous experimental infection demonstrated that juvenile
muskellunge (Esox masquinongy) can survive experimental
infection of viral hemorrhagic septicemia virus, Genotype
IVb (VHSV IVb) at a low concentration of exposure. Herein
we report that survivors of experimental infection with
VHSV IVb shed the virus into the surrounding environment
for an extended period of time. When muskellunge were
exposed to VHSV IVb by immersion at a concentration of
1,400 plaque forming units (PFU)/ml, VHSV IVb was detected
in the water of surviving fish for up to 15 weeks postexposure
(p.e.) with the highest levels of shedding occurring
between weeks 1 and 5 p.e. We estimated that each juvenile
muskellunge can shed upwards of 1.36×105 PFU/fish/h after
initial exposure signifying the uptake and amplification of
VHSV to several orders of magnitude above the original
exposure concentration. Muskellunge surviving low concentration
exposure were re-infected with VHSV IVb by immersion
at week 22 p.e. at concentrations ranging from 0 to
106 PFU/ml. Viral shedding was detected in all re-exposed
fish, including mock rechallenged controls up to 15 consecutive
weeks. Rates of viral shedding were substantially higher
following rechallenge in the first 5 weeks. The highest rate
of viral shedding was approximately 4.6×106 PFU/fish/h and
shedding did not necessarily correspond to the re-exposure
VHSV concentration. The results of this study shed new
light into the dynamics of VHSV IVb shedding in a highly
susceptible host and provide useful insights to fishery managers
to design effective control strategies to this deadly
virus.
Research Support, Non-U.S. Gov'ts
- KSHV Infection of B-Cell Lymphoma Using a Modified KSHV BAC36 and Coculturing System
-
Hyosun Cho , Hyojeung Kang
-
J. Microbiol. 2012;50(2):285-292. Published online April 27, 2012
-
DOI: https://doi.org/10.1007/s12275-012-1495-9
-
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262
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0
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-
6
Crossref
-
Abstract
PDF
-
Kaposi’s sarcoma-associated herpesvirus (KSHV) is the
causative agent of two B cell lymphoproliferative diseases,
namely primary effusion lymphoma (PEL) and multicentric
Castleman’s disease (MCD). KSHV infection of B cell lymphoma
in vitro has been a long-standing battle in advancing
human KSHV biology. In this study, a modified form of
KSHV BAC36 named BAC36A significantly increased the
fidelity of gene-targeted site-directed mutagenesis in the
KSHV genome. This modification eliminates tedious screening
steps required to obtain mutant clones when a KSHV
BAC36 reverse genetic system is used. Coculturing B-cell
lymphoma BJAB cells with KSHV BAC36A stably transfected
293T cells enabled us to infect BJAB cells with a
KSHV virion derived from the KSHV BAC36A. The coculture
system produced substantial amounts of KSHV infection
to BJAB, meaning that KSHV virions were released
from 293T cells and then infected neighboring BJAB cells.
Owing to our success with the KSHV BAC36A and coculture
system, we propose a new genetic system for the study of
KSHV gene expression and regulation in B-cell lymphoma.
-
Citations
Citations to this article as recorded by

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- Antiviral Activities of Flavonoids Isolated from the Bark of Rhus verniciflua Stokes against Fish Pathogenic Viruses In Vitro
-
So Young Kang , Ji-Young Kang , Myung-Joo Oh
-
J. Microbiol. 2012;50(2):293-300. Published online April 27, 2012
-
DOI: https://doi.org/10.1007/s12275-012-2068-7
-
-
231
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0
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-
80
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Abstract
PDF
-
An 80% methanolic extract of Rhus verniciflua Stokes bark
showed significant anti-viral activity against fish pathogenic
infectious hematopoietic necrosis virus (IHNV) and
viral hemorrhagic septicemia virus (VHSV) in a cell-based
assay measuring virus-induced cytopathic effect (CPE).
Activity-guided fractionation and isolation for the 80%
methanolic extract of R. verniciflua yielded the most active
ethyl acetate fraction, and methyl gallate (1) and four flavonoids:
fustin (2), fisetin (3), butin (4) and sulfuretin (5).
Among them, fisetin (3) exhibited high antiviral activities
against both IHNV and VHSV showing EC50 values of 27.1
and 33.3 μM with selective indices (SI = CC50/EC50) more
than 15, respectively. Fustin (2) and sulfuretin (5) displayed
significant antiviral activities showing EC50 values of 91.2–
197.3 μM against IHNV and VHSV. In addition, the antiviral
activity of fisetin against IHNV and VHSV occurred
up to 5 hr post-infection and was not associated with direct
virucidal effects in a timed addition study using a plaque
reduction assay. These results suggested that the bark of R.
verniciflua and isolated flavonoids have significant anti-viral
activity against IHNV and VHSV, and also have potential
to be used as anti-viral therapeutics against fish viral diseases.
-
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Metabolic Engineering.2015; 31: 84. CrossRef - Melatonin Enhances the Anti-Tumor Effect of Fisetin by Inhibiting COX-2/iNOS and NF-κB/p300 Signaling Pathways
Canhui Yi, Yong Zhang, Zhenlong Yu, Yao Xiao, Jingshu Wang, Huijuan Qiu, Wendan Yu, Ranran Tang, Yuhui Yuan, Wei Guo, Wuguo Deng, Chunhong Yan
PLoS ONE.2014; 9(7): e99943. CrossRef - Integrating Traditional Medicine into Modern Inflammatory Diseases Care: Multitargeting byRhus vernicifluaStokes
Ji Hye Kim, Yong Cheol Shin, Seong-Gyu Ko
Mediators of Inflammation.2014; 2014: 1. CrossRef - Phenolic extractives in the trunk of Toxicodendron vernicifluum: chemical characteristics, contents and radial distribution
Koh Hashida, Masanobu Tabata, Katsushi Kuroda, Yuichiro Otsuka, Satoshi Kubo, Rei Makino, Yoshitaka Kubojima, Mario Tonosaki, Seiji Ohara
Journal of Wood Science.2014; 60(2): 160. CrossRef - Antioxidant activity, total phenolic and flavonoid content variation among Tunisian natural populations of Rhus tripartita (Ucria) Grande and Rhus pentaphylla Desf.
Chetoui Itidel, Messaoud Chokri, Boussaid Mohamed, Zaouali Yosr
Industrial Crops and Products.2013; 51: 171. CrossRef - Optimization of total flavonoids content extracted from Flos Populi using response surface methodology
Zun-Lai Sheng, Peng-Fei Wan, Chun-Liu Dong, Yan-Hua Li
Industrial Crops and Products.2013; 43: 778. CrossRef
- Modulation of Immune Response by Interleukin-10 in Systemic Corynebacterium kutscheri Infection in Mice
-
Eui-Suk Jeong , Kyoung-Sun Lee , Seung-Ho Heo , Jin-Hee Seo , Yang-Kyu Choi
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J. Microbiol. 2012;50(2):301-310. Published online April 27, 2012
-
DOI: https://doi.org/10.1007/s12275-012-1298-z
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157
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Interleukin (IL)-10 is an anti-inflammatory cytokine that
modulates sepsis by decreasing pro-inflammatory cytokine
production and chemokine expression. In this study, IL-
10-deficient and wild-type (WT) mice were infected with
Corynebacterium kutscheri to determine if the absence of
IL-10 altered the protective immunity and pathogenesis.
After infection, IL-10 knockout (KO) mice had a higher
survival rate than WT mice. The decrease of body weight
and the increased weight of organs such as liver and spleen
were greater in WT mice. Bacterial counts were significantly
increased after inoculation in WT mice over those in
IL-10 KO mice. WT mice had more granulomatous inflammation
and coagulative necrosis in the liver and spleen,
lymphocyte depletion in lymphoid follicles, and apoptosis
of immune cells in the spleen. WT mice had significantly
higher plasma concentrations of aspartate aminotransferase
and alanine aminotransferase. Furthermore, more upregulation
of tumor necrosis factor-α and IL-4 in the plasma,
macrophage inflammatory protein-2, keratinocyte-derived
chemokine, inducible nitric oxide synthase, and interferoninducible
protein 10 mRNA in the spleen were observed in
WT mice after inoculation. These results suggest that the
lack of IL-10 contributes to an increase in the systemic
clearance of C. kutscheri, and that IL-10 plays a detrimental
role in controlling systemic C. kutscheri infection.
Journal Article
- Porphyromonas gingivalis-Derived Lipopolysaccharide-Mediated Activation of MAPK Signaling Regulates Inflammatory Response and Differentiation in Human Periodontal Ligament Fibroblasts
-
Taegun Seo , Seho Cha , Tae-Il Kim , Hee-Jung Park , Jeong-Soon Lee , Kyung Mi Woo
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J. Microbiol. 2012;50(2):311-319. Published online April 27, 2012
-
DOI: https://doi.org/10.1007/s12275-012-2146-x
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253
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29
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Abstract
PDF
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Porphyromonas gingivalis (P.g.), which is a potential pathogen
for periodontal diseases, contains lipopolysaccharide
(LPS), and this endotoxin stimulates a variety of cellular
responses. At present, P.g.-derived LPS-induced cellular responses
in human periodontal ligament fibroblasts (PDLFs)
are not well characterized. Here, we demonstrate that P.gderived
LPS regulates inflammatory responses, apoptosis
and differentiation in PDLFs. Interleukin-6 (IL-6) and -8
(IL-8) were effectively upregulated by treatment of P.g.-derived
LPS, and we confirmed apoptosis markers including
elevated cytochrome c levels, active caspase-3 and morphological
change in the presence of P.g.-derived LPS. Moreover,
when PDLFs were cultured with differentiation media, P.g.-
derived LPS reduced the expression of differentiation marker
genes, as well as reducing alkaline phosphatase (ALP) activity
and mineralization. P.g.-derived LPS-mediated these
cellular responses were effectively abolished by treatment
of mitogen-activated protein kinase (MAPK) inhibitors.
Taken together, our results suggest that P.g.-derived LPS
regulates several cellular responses via activation of MAPK
signaling pathways in PDLFs.
-
Citations
Citations to this article as recorded by

- Biofilms and oral health: nanotechnology for biofilm control
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Discover Nano.2025;[Epub] CrossRef - Evaluation of the role of mitofusin‐1 and mitofusin‐2 in periodontal disease
Ömer Alperen Kırmızıgül, Arife Sabanci, Faruk Dişli, Sedat Yıldız, Michael R. Milward, Kübra Aral
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Wei He, Yu Fu, Song Yao, Lan Huang
Journal of Cellular Physiology.2023; 238(8): 1768. CrossRef - Porphyromonas gingivalis lipopolysaccharide promotes T-hel per17 cell differentiation by upregulating Delta-like ligand 4 expression on CD14+ monocytes
Chi Zhang, Chenrong Xu, Li Gao, Xiting Li, Chuanjiang Zhao
PeerJ.2021; 9: e11094. CrossRef - Gene expression profiles of mitochondria-endoplasmic reticulum tethering in human gingival fibroblasts in response to periodontal pathogens
Kübra Aral, Michael R. Milward, Paul R. Cooper
Archives of Oral Biology.2021; 128: 105173. CrossRef - Ginsenoside Rb3 Inhibits Pro-Inflammatory Cytokines via MAPK/AKT/NF-κB Pathways and Attenuates Rat Alveolar Bone Resorption in Response to Porphyromonas gingivalis LPS
Minmin Sun, Yaoting Ji, Zhen Li, Rourong Chen, Shuhui Zhou, Chang Liu, Minquan Du
Molecules.2020; 25(20): 4815. CrossRef - Low-intensity pulsed ultrasound upregulates osteogenesis under inflammatory conditions in periodontal ligament stem cells through unfolded protein response
Han Li, Yuejia Deng, Minmin Tan, Ge Feng, Yunchun Kuang, Jie Li, Jinlin Song
Stem Cell Research & Therapy.2020;[Epub] CrossRef - Effect of ScLL and 15d-PGJ2 on viability and cytokine release in LPS-stimulated fibroblasts: an in vitro study
Manuella Verdinelli de Paula REIS, Gabriela Leite de SOUZA, Priscilla Barbosa Ferreira SOARES, Maria Aparecida de SOUZA, Carlos José SOARES, Camilla Christian Gomes MOURA
Brazilian Oral Research.2020;[Epub] CrossRef - TLR activation inhibits the osteogenic potential of human periodontal ligament stem cells through Akt signaling in a Myd88‐ or TRIF‐dependent manner
Yunyan Zhu, Qian Li, Yanheng Zhou, Weiran Li
Journal of Periodontology.2019; 90(4): 400. CrossRef - Low‐intensity pulsed ultrasound promotes bone morphogenic protein 9‐induced osteogenesis and suppresses inhibitory effects of inflammatory cytokines on cellular responses via Rho‐associated kinase 1 in human periodontal ligament fibroblasts
Joji Kusuyama, Toshiaki Nakamura, Tomokazu Ohnishi, Brent G. Albertson, Yukari Ebe, Nahoko Eiraku, Kazuyuki Noguchi, Tetsuya Matsuguchi
Journal of Cellular Biochemistry.2019; 120(9): 14657. CrossRef - Periodontal bacterial supernatants modify differentiation, migration and inflammatory cytokine expression in human periodontal ligament stem cells
Liza L. Ramenzoni, Giancarlo Russo, Maria D. Moccia, Thomas Attin, Patrick R. Schmidlin, Alain Haziot
PLOS ONE.2019; 14(7): e0219181. CrossRef - Necrostatin-1 promotes ectopic periodontal tissue like structure regeneration in LPS-treated PDLSCs
Bingbing Yan, Hongmei Zhang, Taiqiang Dai, Yongchun Gu, Xinyu Qiu, Cheng Hu, Yan Liu, Kewen Wei, Dehua Li, Motohiro Komaki
PLOS ONE.2018; 13(11): e0207760. CrossRef - TGF-β2 downregulates osteogenesis under inflammatory conditions in dental follicle stem cells
Soyoun Um, Joo-Hee Lee, Byoung-Moo Seo
International Journal of Oral Science.2018;[Epub] CrossRef - Periostin promotes migration and osteogenic differentiation of human periodontal ligament mesenchymal stem cells via the Jun amino‐terminal kinases (JNK) pathway under inflammatory conditions
Yi Tang, Lin Liu, Pei Wang, Donglei Chen, Ziqiang Wu, Chunbo Tang
Cell Proliferation.2017;[Epub] CrossRef - Osteogenic potential of periodontal ligament stem cells are unaffected after exposure to lipopolysaccharides
Mayra Laino ALBIERO, Bruna Rabelo AMORIM, Márcio Zaffalon CASATI, Enilson Antonio SALLUM, Francisco Humberto NOCITI JUNIOR, Karina Gonzales SILVÉRIO
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Shujuan Guo, Jian Kang, Baohui Ji, Weihua Guo, Yi Ding, Yafei Wu, Weidong Tian
Tissue Engineering Part A.2017; 23(13-14): 585. CrossRef - Hypoxia enhances the effect of lipopolysaccharide-stimulated IL-1β expression in human periodontal ligament cells
Jittima Pumklin, Kanokporn Bhalang, Prasit Pavasant
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Eduardo Anitua, Mar Zalduendo, María Troya, Sabino Padilla, Gorka Orive, Paula A. da Costa Martins
PLOS ONE.2015; 10(3): e0121713. CrossRef - The negative feedback regulation of microRNA-146a in human periodontal ligament cells after Porphyromonas gingivalis lipopolysaccharide stimulation
Shao-Yun Jiang, Dong Xue, Yu-Feng Xie, Dong-Wang Zhu, Yun-Yun Dong, Cong-Cong Wei, Jia-Yin Deng
Inflammation Research.2015; 64(6): 441. CrossRef - IL-1R/TLR2 through MyD88 Divergently Modulates Osteoclastogenesis through Regulation of Nuclear Factor of Activated T Cells c1 (NFATc1) and B Lymphocyte-induced Maturation Protein-1 (Blimp1)
Zhihong Chen, Lingkai Su, Qingan Xu, Jenny Katz, Suzanne M. Michalek, Mingwen Fan, Xu Feng, Ping Zhang
Journal of Biological Chemistry.2015; 290(50): 30163. CrossRef - Effects of Streptococcus thermophilus on volatile sulfur compounds produced by Porphyromonas gingivalis
Sung-Hoon Lee, Dong-Heon Baek
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Hirohito Kato, Yoichiro Taguchi, Kazuya Tominaga, Makoto Umeda, Akio Tanaka
Archives of Oral Biology.2014; 59(2): 167. CrossRef - Effect of epithelial rests of Malassez’ cells on RANKL mRNA expression and ALP activity by periodontal ligament fibroblasts stimulated with sonicated Porphyromonas gingivalis in vitro
Kenichi Matsuzaka, Eitoyo Kokubu, Takashi Inoue
Journal of Oral and Maxillofacial Surgery, Medicine, and Pathology.2014; 26(4): 554. CrossRef - Effects of Enterococcus faecalis lipoteichoic acid on receptor activator of nuclear factor‐κB ligand and osteoprotegerin expression in periodontal ligament fibroblasts
L. Zhao, J. Chen, L. Cheng, X. Wang, J. Du, F. Wang, Z. Peng
International Endodontic Journal.2014; 47(2): 163. CrossRef - Apoptosis: an underlying factor for accelerated periodontal disease associated with diabetes in rats
Mustafa Tunalı, Tamer Ataoğlu, Ilhami Çelik
Clinical Oral Investigations.2014; 18(7): 1825. CrossRef - Reducing the bioactivity of Tannerella forsythia lipopolysaccharide by Porphyromonas gingivalis
Young-Jae Kim, Sung-Hoon Lee
Journal of Microbiology.2014; 52(8): 702. CrossRef - Bambusae Caulis in Taeniam modulates neuroprotective and anti-neuroinflammatory effects in hippocampal and microglial cells via HO-1- and Nrf-2-mediated pathways
HYE WON EOM, SUN YOUNG PARK, YOUNG HUN KIM, SU JIN SEONG, MEI LING JIN, EUN YEON RYU, MIN JU KIM, SANG JOON LEE
International Journal of Molecular Medicine.2012; 30(6): 1512. CrossRef - Baicalin Downregulates Porphyromonas gingivalis Lipopolysaccharide-Upregulated IL-6 and IL-8 Expression in Human Oral Keratinocytes by Negative Regulation of TLR Signaling
Wei Luo, Cun-Yu Wang, Lijian Jin, Anne Wertheimer
PLoS ONE.2012; 7(12): e51008. CrossRef
Research Support, Non-U.S. Gov'ts
- Cyclic AMP-Receptor Protein Activates Aerobactin Receptor IutA Expression in Vibrio vulnificus
-
Choon-Mee Kim , Seong-Jung Kim , Sung-Heui Shin
-
J. Microbiol. 2012;50(2):320-325. Published online April 27, 2012
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DOI: https://doi.org/10.1007/s12275-012-2056-y
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168
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10
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Abstract
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The ferrophilic bacterium Vibrio vulnificus can utilize the siderophore aerobactin of Escherichia coli for iron acquisition via its specific receptor IutA. This siderophore piracy by V. vulnificus may contribute to its survival and proliferation, especially in mixed bacterial environments. In this study, we examined the effects of glucose, cyclic AMP (cAMP), and cAMP-receptor protein (Crp) on iutA expression in V. vulnificus. Glucose dose-dependently repressed iutA expression. A mutation in cya encoding adenylate cyclase required for cAMP synthesis severely repressed iutA expression, and this change was recovered by in trans complementing cya or the addition of exogenous cAMP. Furthermore, a mutation in crp encoding Crp severely repressed iutA expression, and this change was recovered by complementing crp. Accordingly, glucose deprivation under iron-limited conditions is an environmental signal for iutA expression, and Crp functions as an activator that regulates iutA expression in response to glucose availability.
- Expression and Purification of Lacticin Q by Small Ubiquitin-Related Modifier Fusion in Escherichia coli
-
Qingshan Ma , Zhanqiao Yu , Bing Han , Qing Wang , Rijun Zhang
-
J. Microbiol. 2012;50(2):326-331. Published online April 27, 2012
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DOI: https://doi.org/10.1007/s12275-012-1425-x
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169
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13
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Abstract
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Lacticin Q is a broad-spectrum class II bacteriocin with potential as an alternative to conventional antibiotics. The objective of this study was to produce recombinant lacticin Q using a small ubiquitin-related modifier (SUMO) fusion protein expression system. The 168-bp lacticin Q gene was cloned into the expression vector pET SUMO and transformed into Escherichia coli BL21(DE3). The soluble fusion protein was recovered with a Ni-NTA Sepharose column (95% purity); 130 mg protein was obtained per liter of fermentation culture. The SUMO tag was then proteolytically cleaved from the protein, which was re-applied to the column. Finally, about 32 mg lacticin Q (≥96% purity) was obtained. The recombinant protein exhibited antimicrobial properties similar to that of the native protein, demonstrating that lacticin Q had been successfully expressed by the SUMO fusion system.
- Heterologous Expression of Polygalacturonase Genes Isolated from Galactomyces citri-aurantii IJ-1 in Pichia pastoris
-
Il Jae Cho , In-Cheol Yeo , Nam Keun Lee , Suk Hee Jung , Young Tae Hahm
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J. Microbiol. 2012;50(2):332-340. Published online April 27, 2012
-
DOI: https://doi.org/10.1007/s12275-012-1290-7
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220
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3
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Abstract
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The objective of this work was to isolate the polygalacturonase
genes of Galactomyces citri-aurantii IJ-1 harvested
from rotten citrus peels and to heterologously express these
genes in Pichia pastoris. Two polygalacturonase (PG) genes
from G. citri-aurantii IJ-1 were obtained and tentatively
named PG1 and PG2. The genes were cloned into pPICZαC,
and expressed in Pichia pastoris strain GS115 with a native
signal peptide or the α-factor secretion signal peptide of
Saccharomyces cerevisiae. All of the recombinant proteins
were successfully secreted into the culture media and confirmed
as a single band with a molecular weight of 35 to 38
kDa by SDS-PAGE. The specific enzyme activities of recombinant
PG1 and PG2 purified by His-tag affinity resin
were 4,749 and 6,719 U/mg, respectively, with an optimal
pH and temperature of pH 4.0 and 50°C. The Michaelis-
Menten kinetic constants for PG1 and PG2, Km, were confirmed
to be 0.94 and 0.84 mM, respectively. In the presence
of Mn2+, the activity of PG1 and PG2 were increased to
160.8 and 146.4% of normal levels, respectively. In contrast,
Cu2+ and Fe3+ acted as strong inhibitors to the PGs.
-
Citations
Citations to this article as recorded by

- Integrated in vitro fermentation, transcriptomics and enzymatic property analyses uncover interaction between polysaccharide from the fruits of Lycium barbarum and Bacteroides ovatus
Jinghong Wei, Wangting Zhou, Wei Yi, Jiameng Shi, Yamei Yan, Jia Mi, Lu Lu, Youlong Cao, Xiaoxiong Zeng
International Journal of Biological Macromolecules.2025; 316: 144786. CrossRef - From Secretion in Pichia pastoris to Application in Apple Juice Processing: Exo-Polygalacturonase from Sporothrix schenckii 1099-18
Ersin Karataş, Ahmet Tülek, Mehmet Mervan Çakar, Faruk Tamtürk, Fatih Aktaş, Barış Binay
Protein & Peptide Letters.2021; 28(7): 817. CrossRef - Expression, purification and characterization of human interferon-γ in Pichia pastoris
DAN WANG, HUI REN, JING-WEI XU, PENG-DA SUN, XUE-DONG FANG
Molecular Medicine Reports.2014; 9(2): 715. CrossRef
- Remodeling of the Glycosylation Pathway in the Methylotrophic Yeast Hansenula polymorpha to Produce Human Hybrid-Type N-Glycans
-
Seon Ah Cheon , Hyunah Kim , Doo-Byoung Oh , Ohsuk Kwon , Hyun Ah Kang
-
J. Microbiol. 2012;50(2):341-348. Published online April 27, 2012
-
DOI: https://doi.org/10.1007/s12275-012-2097-2
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191
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28
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Abstract
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As a step forward to achieve the generation of human complex-
type N-glycans in the methylotrophic yeast Hansenula
polymorpha, we here report the modification of the yeast
glycosylation pathway by heterologous expression of the
human gene encoding β-1,2-N-acetylglucosaminyltransferase
I (GnTI). For the optimal expression of human GnTI in
the yeast Golgi compartment, the catalytic domain of the
GnTI was fused to various N-terminal leader sequences derived
from the yeast type II membrane proteins. The vectors
containing GnTI fusion constructs were introduced into
the H. polymorpha och1Δ single and och1Δalg3Δ double
mutant strains expressing the ER-targeted Aspergillus saitoi
α-1,2 mannosidase, respectively. Both of the glycoengineered
Hpoch1Δ and Hpoch1ΔHpalg3Δ strains were shown to produce
successfully the hybrid-type glycans with a monoantennary
N-acetylglucosamine (GlcNAc1Man5GlcNAc2 and
GlcNAc1Man3GlcNAc2, respectively) by N-glycan profile
analysis of cell wall proteins. Furthermore, by comparative
analysis of byproduct formation and the glycosylation site
occupancy, we propose that the Hpoch1Δ strain would be
more suitable than the Hpoch1ΔHpalg3Δ strain as a host
for the production of recombinant proteins with humanized
glycans.
-
Citations
Citations to this article as recorded by

- Recent progress on heterologous protein production in methylotrophic yeast systems
Masashi Tsuda, Koichi Nonaka
World Journal of Microbiology and Biotechnology.2024;[Epub] CrossRef - Yeast and Virus-like Particles: A Perfect or Imperfect Couple?
Sara Brachelente, Alvaro Galli, Tiziana Cervelli
Applied Microbiology.2023; 3(3): 805. CrossRef - Production of galactosylated complex-type N-glycans in glycoengineered Saccharomyces cerevisiae
Mari A. Piirainen, Heidi Salminen, Alexander D. Frey
Applied Microbiology and Biotechnology.2022; 106(1): 301. CrossRef - Humanization of Yeasts for Glycan-Type End-Products
Xingjuan Li, Jianlie Shen, Xingqiang Chen, Lei Chen, Shulin Wan, Xingtao Qiu, Ke Chen, Chunmiao Chen, Haidong Tan
Frontiers in Microbiology.2022;[Epub] CrossRef - Glycoengineering of Aspergillus nidulans to produce precursors for humanized N-glycan structures
Diana Chinyere Anyaogu, Anders Holmgaard Hansen, Jakob Blæsbjerg Hoof, Natalia I. Majewska, Fabiano Jares Contesini, Jackson T. Paul, Kristian Fog Nielsen, Timothy John Hobley, Shuang Yang, Hui Zhang, Michael Betenbaugh, Uffe Hasbro Mortensen
Metabolic Engineering.2021; 67: 153. CrossRef - EngineeringYarrowia lipolyticaforde novoproduction of tetraacetyl phytosphingosine
Changpyo Han, Minjeong Jang, Min Ju Kim, Man‐Ho Han, Kyeong‐Ryoon Lee, Ji‐Sook Hahn, Jungoh Ahn
Journal of Applied Microbiology.2021; 130(6): 1981. CrossRef - Customized yeast cell factories for biopharmaceuticals: from cell engineering to process scale up
Aravind Madhavan, K. B. Arun, Raveendran Sindhu, Jayaram Krishnamoorthy, R. Reshmy, Ranjna Sirohi, Arivalagan Pugazhendi, Mukesh Kumar Awasthi, George Szakacs, Parameswaran Binod
Microbial Cell Factories.2021;[Epub] CrossRef - Constructing a human complex type N-linked glycosylation pathway in Kluyveromyces marxianus
Ming-Hsuan Lee, Tsui-Ling Hsu, Jinn-Jy Lin, Yu-Ju Lin, Yi-Ying Kao, Jui-Jen Chang, Wen-Hsiung Li, Chih-Pin Chuu
PLOS ONE.2020; 15(5): e0233492. CrossRef - Investigating the role of ERAD on antibody processing in glycoengineeredSaccharomyces cerevisiae
Mari A Piirainen, Alexander D Frey
FEMS Yeast Research.2020;[Epub] CrossRef - Yeast synthetic biology for designed cell factories producing secretory recombinant proteins
Eun Jung Thak, Su Jin Yoo, Hye Yun Moon, Hyun Ah Kang
FEMS Yeast Research.2020;[Epub] CrossRef - Advances in Using Hansenula polymorpha as Chassis for Recombinant Protein Production
João Heitor Colombelli Manfrão-Netto, Antônio Milton Vieira Gomes, Nádia Skorupa Parachin
Frontiers in Bioengineering and Biotechnology.2019;[Epub] CrossRef - Structural analysis of N-/O-glycans assembled on proteins in yeasts
Eun Jung Thak, Jungho Kim, Dong-Jik Lee, Jeong Yoon Kim, Hyun Ah Kang
Journal of Microbiology.2018; 56(1): 11. CrossRef - Humanizing glycosylation pathways in eukaryotic expression systems
Amjad Hayat Khan, Hadi Bayat, Masoumeh Rajabibazl, Suriana Sabri, Azam Rahimpour
World Journal of Microbiology and Biotechnology.2017;[Epub] CrossRef - Reexamining opportunities for therapeutic protein production in eukaryotic microorganisms
Catherine B. Matthews, Chapman Wright, Angel Kuo, Noelle Colant, Matthew Westoby, J. Christopher Love
Biotechnology and Bioengineering.2017; 114(11): 2432. CrossRef - Effects of Rho1, a small GTPase on the production of recombinant glycoproteins in Saccharomyces cerevisiae
Sha Xu, Ge-Yuan Zhang, Huijie Zhang, Toshihiko Kitajima, Hideki Nakanishi, Xiao-Dong Gao
Microbial Cell Factories.2016;[Epub] CrossRef - Synthetic biology and molecular genetics in non-conventional yeasts: Current tools and future advances
James M. Wagner, Hal S. Alper
Fungal Genetics and Biology.2016; 89: 126. CrossRef - Molecular Characterization of Hap Complex Components Responsible for Methanol-Inducible Gene Expression in the Methylotrophic Yeast Candida boidinii
Saori Oda, Hiroya Yurimoto, Nobuhisa Nitta, Yu Sasano, Yasuyoshi Sakai
Eukaryotic Cell.2015; 14(3): 278. CrossRef - Hansenula polymorpha Pmt4p Plays Critical Roles in O-Mannosylation of Surface Membrane Proteins and Participates in Heteromeric Complex Formation
Hyunah Kim, Eun Jung Thak, Dong-Jik Lee, Michael O. Agaphonov, Hyun Ah Kang, Alvaro Galli
PLOS ONE.2015; 10(7): e0129914. CrossRef -
Hansenula polymorpha Hac1p Is Critical to Protein
N
-Glycosylation Activity Modulation, as Revealed by Functional and Transcriptomic Analyses
Hye-Yun Moon, Seon Ah Cheon, Hyunah Kim, M. O. Agaphonov, Ohsuk Kwon, Doo-Byoung Oh, Jeong-Yoon Kim, Hyun Ah Kang, D. Cullen
Applied and Environmental Microbiology.2015; 81(20): 6982. CrossRef - Manipulating the glycosylation pathway in bacterial and lower eukaryotes for production of therapeutic proteins
Diana Chinyere Anyaogu, Uffe Hasbro Mortensen
Current Opinion in Biotechnology.2015; 36: 122. CrossRef - Overexpression of membrane proteins from higher eukaryotes in yeasts
Anita Emmerstorfer, Tamara Wriessnegger, Melanie Hirz, Harald Pichler
Applied Microbiology and Biotechnology.2014; 98(18): 7671. CrossRef - Characterization of putative glycosylphosphatidylinositol-anchoring motifs for surface display in the methylotrophic yeast Hansenula polymorpha
Seon Ah Cheon, Jinhee Jung, Jin Ho Choo, Doo-Byoung Oh, Hyun Ah Kang
Biotechnology Letters.2014; 36(10): 2085. CrossRef - Biopharmaceutical discovery and production in yeast
Michael A Meehl, Terrance A Stadheim
Current Opinion in Biotechnology.2014; 30: 120. CrossRef - Novel Cysteine-Centered Sulfur Metabolic Pathway in the Thermotolerant Methylotrophic Yeast Hansenula polymorpha
Min Jeong Sohn, Su Jin Yoo, Doo-Byoung Oh, Ohsuk Kwon, Sang Yup Lee, Andriy A. Sibirny, Hyun Ah Kang, Utpal Sen
PLoS ONE.2014; 9(6): e100725. CrossRef - Yeast synthetic biology for the production of recombinant therapeutic proteins
Hyunah Kim, Su Jin Yoo, Hyun Ah Kang
FEMS Yeast Research.2014; : n/a. CrossRef - Stable overproducer of hepatitis B surface antigen in the methylotrophic yeast Hansenula polymorpha due to multiple integration of heterologous auxotrophic selective markers and defect in peroxisome biogenesis
Olena S. Krasovska, Oleh V. Stasyk, Andriy A. Sibirny
Applied Microbiology and Biotechnology.2013; 97(23): 9969. CrossRef - Functional and molecular characterization of novel Hansenula polymorpha genes, HpPMT5 and HpPMT6, encoding protein O-mannosyltransferases
Hyunah Kim, Hye Yun Moon, Dong-jik Lee, Seon Ah Cheon, Su Jin Yoo, Jeong-Nam Park, Michael O. Agaphonov, Doo-Byoung Oh, Ohsuk Kwon, Hyun Ah Kang
Fungal Genetics and Biology.2013; 58-59: 10. CrossRef - Expression of glycoproteins bearing complex human-like glycans with galactose terminal in Hansenula polymorpha
Hui Wang, Hao-lei Song, Qian Wang, Bing-sheng Qiu
World Journal of Microbiology and Biotechnology.2013; 29(3): 447. CrossRef
- NOTE] Phycicoccus ochangensis sp. nov., Isolated from Soil of a Potato Cultivation Field
-
Hyangmi Kim , Hyun-Woo Oh , Doo-Sang Park , Kang Hyun Lee , Sung Uk Kim , Hee-Moon Park , Kyung Sook Bae
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J. Microbiol. 2012;50(2):349-353. Published online April 27, 2012
-
DOI: https://doi.org/10.1007/s12275-012-1206-6
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Two novel, Gram-positive, motile, coccal bacteria, strains L1b-b9T and B5a-b5, were isolated from a potato cultivation field in Ochang, Korea. These isolates grew at 10–45°C, pH 5.0–10.0, and in the presence of 8% (w/v) NaCl. The diagnostic diamino acid in the cell-wall peptidoglycan was mesodiaminopimelic acid. The major menaquinone was MK-8(H4) and the main cellular fatty acids were iso-C14:0, iso-C15:0, and anteiso-C15:0. Polar lipids in strain L1b-b9T consisted of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol, and an unknown glyco-amino lipid. The G+C content of genomic DNA was 73.6 mol%. A phylogenetic analysis based on 16S rRNA gene sequences showed that strains L1b-b9T and B5a-b5 shared 99.36% similarity and formed a robust clade with the type species of the genus Phycicoccus. Strain L1b-b9T is related most closely to Phycicoccus cremeus V2M29T (97.52% 16S rRNA gene sequence similarity). On the basis of phylogenetic characteristics, the name Phycicoccus ochangensis sp. nov. is proposed for strain LIb-b9T (=KCTC 19694T =JCM 17595T).
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- Phycicoccus sonneraticus sp. nov., a Novel Endophytic Actinobacterium Isolated from the Bark of Sonneratia apetala
Huiling Tang, Xiaohui Chen, Mingsheng Chen, Xiaohong Li, Jianjing Jiang, Li Tuo, Feina Li
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Udit Yadav, Nasreen Bano, Sumit Bag, Suchi Srivastava, Poonam C. Singh, Jeffrey A. Gralnick
Microbiology Spectrum.2022;[Epub] CrossRef - Phycicoccus ginsengisoli sp. nov., isolated from cultivated ginseng soil
Jong-Pyo Kang, Yeon-Ju Kim, Ngoc-Lan Nguyen, Van-An Hoang, Mohamed El-Agamy Farh, Sung-Chul Joo, Lin-Hu Quan, Deok-Chun Yang
International Journal of Systematic and Evolutionary Microbiology.2016; 66(12): 5320. CrossRef -
Phycicoccus soli sp. nov., isolated from soil
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Journal Article
- NOTE] Arenimonas aquaticum sp. nov., a Member of the Gammaproteobacterium, Isolated from a Freshwater Reservoir
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A-Ram Kim , Siwon Lee , Kyudong Han , Tae-Young Ahn
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J. Microbiol. 2012;50(2):354-358. Published online April 27, 2012
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DOI: https://doi.org/10.1007/s12275-012-1301-8
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A novel bacterial strain, designated NA-09T, was isolated
from a freshwater sample collected from the Cheonho reservoir,
Republic of Korea. Colonies were creamy-white pigmented,
translucent, and circular with convex shape. The
isolate was Gram-staining negative, strictly aerobic, motile,
and rod-shaped. The 16S rRNA gene sequence analysis revealed
that strain NA-09T belonged to the genus Arenimonas
and showed the highest sequence similarities with Arenimonas
malthae CC-JY-1T (95.4%), A. oryziterrae YC6267T
(94.9%), A. composti P2-12-1T (94.8%), and A. donghaensis
H03-R19T (94.1%). The major fatty acids were iso-C16:0
(20.8%), iso-C15:0 (16.9%), summed feature 1 (13.2%), and
iso-C16:1ω7c alcohol (10.2%). The major isoprenoid quinone
of the isolate was ubiquionone-8. On the basis of the data
from the polyphasic characterization, the strain NA-09T
represents a novel species, for which the name Arenimonas
aquaticum sp. nov. is proposed (type strain NA-09T =KACC
14663T =NBRC 106550T).
Research Support, Non-U.S. Gov't
- NOTE] GFP-Expressing Influenza A Virus for Evaluation of the Efficacy of Antiviral Agents
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Jin Il Kim , Sehee Park , Ilseob Lee , Sangmoo Lee , Saem Shin , Yongkwan Won , Min-Woong Hwang , Joon-Yong Bae , Jun Heo , Hye-Eun Hyun , Hyejin Jun , Soon Sung Lim , Man-Seong Park
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J. Microbiol. 2012;50(2):359-362. Published online April 27, 2012
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DOI: https://doi.org/10.1007/s12275-012-2163-9
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197
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14
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To address its value as a screening tool in the development of antiviral drugs, a recombinant influenza virus expressing green fluorescent protein (rPR8-GFP virus) was investigated in vitro and in vivo. The inhibition of viral growth by a neuraminidase inhibitor in the cells or lower respiratory tracts of mice could be visualized by the level of fluorescence. In addition, the rPR8-GFP virus exhibited high pathogenicity in mice. Taken together, these results suggest that the rPR8-GFP virus can be a useful tool for the rapid identification of antiviral drugs active against influenza viruses.
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Citations
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Rak-Kyun Seong, Young-Ki Choi, Ok Sarah Shin
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Michael Breen, Aitor Nogales, Steven Baker, Luis Martínez-Sobrido
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Daniela Toro-Ascuy, Carolina Tambley, Carolina Beltran, Carolina Mascayano, Nicolas Sandoval, Eduardo Olivares, Rafael A. Medina, Eugenio Spencer, Marcelo Cortez-San Martín, M. W. Griffiths
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Sangmoo Lee, Jin Il Kim, Ilseob Lee, Man-Seong Park
Journal of Bacteriology and Virology.2013; 43(4): 337. CrossRef - The anti-influenza virus effect of Phellinus igniarius extract
Sangmoo Lee, Jin Il Kim, Jun Heo, Ilseob Lee, Sehee Park, Min-Woong Hwang, Joon-Yong Bae, Mee Sook Park, Hyoung Jin Park, Man-Seong Park
Journal of Microbiology.2013; 51(5): 676. CrossRef - Aronia melanocarpa and its components demonstrate antiviral activity against influenza viruses
Sehee Park, Jin Il Kim, Ilseob Lee, Sangmoo Lee, Min-Woong Hwang, Joon-Yong Bae, Jun Heo, Donghwan Kim, Sang-Zin Han, Man-Seong Park
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Sehee Park, Jin Il Kim, Ilseob Lee, Sangmoo Lee, Min-Woong Hwang, Joon-Yong Bae, Jun Heo, Eun-Joo Lim, Won-Seok Seok, Hee Jin Cheong, Joon Young Song, Woo Joo Kim, Man-Seong Park
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- ERRATUM] Neutralization Potential of the Plasma of HIV-1 Infected Indian Patients in the Context of Anti-V3 Antibody Content and Antiretroviral Theraphy
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Alok Kumar Choudhary , Raiees Andrabi , Somi Sankaran Prakash , Rajesh Kumar , Shubhasree Dutta Choudhury , Naveet Wig , Ashutosh Biswas , Anjali Hazarika , Kalpana Luthra
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J. Microbiol. 2012;50(2):363-363.
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In the article by Choudhary et al. that appears in the Journal of Microbiology 2012; 50, 149-154. Page 149, on journal title ‘Theraphy’ should read ‘Therapy’.