- Mycobacterium tuberculosis PE_PGRS45 (Rv2615c) Promotes Recombinant Mycobacteria Intracellular Survival via Regulation of Innate Immunity, and Inhibition of Cell Apoptosis
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Tao Xu , Chutong Wang , Minying Li , Jing Wei , Zixuan He , Zhongqing Qian , Xiaojing Wang , Hongtao Wang
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J. Microbiol. 2024;62(1):49-62. Published online February 9, 2024
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DOI: https://doi.org/10.1007/s12275-023-00101-0
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 Abstract
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Tuberculosis (TB), a bacterial infectious disease caused by Mycobacterium tuberculosis (M. tuberculosis), is a significant
global public health problem. Mycobacterium tuberculosis expresses a unique family of PE_PGRS proteins that have been
implicated in pathogenesis. Despite numerous studies, the functions of most PE_PGRS proteins in the pathogenesis of
mycobacterium infections remain unclear. PE_PGRS45 (Rv2615c) is only found in pathogenic mycobacteria. In this study,
we successfully constructed a recombinant Mycobacterium smegmatis (M. smegmatis) strain which heterologously expresses
the PE_PGRS45 protein. We found that overexpression of this cell wall-associated protein enhanced bacterial viability under
stress in vitro and cell survival in macrophages. MS_PE_PGRS45 decreased the secretion of pro-inflammatory cytokines such
as IL-1β, IL-6, IL-12p40, and TNF-α. We also found that MS_PE_PGRS45 increased the expression of the anti-inflammatory
cytokine IL-10 and altered macrophage-mediated immune responses. Furthermore, PE_PGRS45 enhanced the survival rate
of M. smegmatis in macrophages by inhibiting cell apoptosis. Collectively, our findings show that PE_PGRS45 is a virulent
factor actively involved in the interaction with the host macrophage.
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Citations
Citations to this article as recorded by  - Evolution of the PE_PGRS Proteins of Mycobacteria: Are All Equal or Are Some More Equal than Others?
Bei Chen, Belmin Bajramović, Bastienne Vriesendorp, Herman Pieter Spaink
Biology.2025; 14(3): 247. CrossRef - Recent advances in research on Mycobacterium tuberculosis virulence factors and their role in pathogenesis
Ming-Rui Sun, Jia-Yin Xing, Xiao-Tian Li, Ren Fang, Yang Zhang, Zhao-Li Li, Ning-Ning Song
Journal of Microbiology, Immunology and Infection.2025; 58(5): 497. CrossRef - Rv2741 Promotes Mycobacterium Survival by Modulating Macrophage Function via the IL-1α-MAPK Axis
Xintong He, Yonglin He, Xichuan Deng, Nan Lu, Anlong Li, Sijia Gao, Shiyan He, Yuran Wang, Nanzhe Fu, Zijie Wang, Yuxin Nie, Lei Xu
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Zhijing Zhang, Le Dong, Xin Li, Taibing Deng, Qinglan Wang
Frontiers in Immunology.2025;[Epub] CrossRef
- Exploring the oral microflora of preschool children
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Wen Ren , Qun Zhang , Xuenan Liu , Shuguo Zheng , Lili Ma , Feng Chen , Tao Xu , Baohua Xu
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J. Microbiol. 2017;55(7):531-537. Published online April 22, 2017
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DOI: https://doi.org/10.1007/s12275-017-6474-8
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579
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Abstract
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The oral cavity is one of the most important and complicated
habitats in our body and supports diverse microbial communities.
In this study, we aimed to determine the bacterial diversity
and composition of various oral micro-niches. Samples
were collected from supragingival plaque, saliva, and tongue
coating from 10 preschool children (30 samples total). 16S
rRNA gene pyrosequencing dataset generated 314,639 clean
reads with an average of 10,488 ± 2,787 reads per sample. The
phyla Firmicutes, Proteobacteria, Actinobacteria, Bacteroidetes,
and Fusobacteria were predominant, accounting for
more than 90% of the total sequences. We found the highest
α diversity, microbial richness, and evenness in plaque, compared
with saliva and tongue coating. Plaque was also distinguished
from saliva and tongue coating by phylogenetic
distances (weighted UniFrac). Taxa with different relative
abundances were further identified, confirming the existence
of microbial differences across the three niches. Core microbiomes
were defined of each niche; however, only a small proportion
of operational taxonomic units (8.07%) were shared
by the three niches. Coaggregation between Actinomyces spp.
and Streptococcus spp. and other correlations among periodontal
pathogens, such as Prevotella, Fusobacteria, Capnocytophaga,
and Tannerella, were shown by a co-occurrence
network. In summary, our study provides a framework of oral
microbial communities in the population of preschool children
as a baseline for further studies of oral diseases related
to microbes.
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Citations
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