- Development of a real-time loop-mediated isothermal amplification method for the detection of severe fever with thrombocytopenia syndrome virus
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Jae Woong Lee , Yu-Jung Won , Lae Hyung Kang , Sung-Geun Lee , Seung-Won Park , Soon-Young Paik
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J. Microbiol. 2020;58(8):711-715. Published online May 18, 2020
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DOI: https://doi.org/10.1007/s12275-020-0109-1
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 Abstract
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Severe fever with thrombocytopenia syndrome (SFTS) is being
reported annually in South Korea since its first detection
there in 2010. The causal agent is a negative-strand RNA
virus 80–100 nm in diameter. It causes fever, thrombocytopenia,
leukocytopenia, gastrointestinal symptoms, and neural
symptoms. The mortality rate of SFTS was 32.6% among 172
case
s reported from 2012 to 2015 in South Korea. Thus, is
necessary to develop an effective diagnostic method that selectively
identifies the isolates circulating in South Korea. The
real-time reverse transcription loop-mediated isothermal amplification
(RT-LAMP) assay is a simple, rapid, and sensitive
approach for molecular diagnosis. Here, we designed novel
primers for this assay and found that the technique had very
high specificity, sensitivity, and efficiency. This real-time RTLAMP
approach using the novel primers developed herein
can be applied for early diagnosis of SFTSV strains in South
Korea to reduce the mortality rate of SFTS.
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Citations
Citations to this article as recorded by  - CRISPR/Cas12a Technology Combined with Immunochromatographic Strips for the Portable Detection of SFTS Bunyavirus
Hao Tang, Yuqing Xing, Guoping Lu, Jilu Shen, Qiang Zhou
Journal of Microbiology and Biotechnology.2026;[Epub] CrossRef - Establishment of a rapid detection method for severe fever with thrombocytopenia syndrome virus based on reverse transcription-recombinase polymerase amplification with lateral flow nucleic acid chromatography assay technology
Zhoujie Ma, Yongchuan Gan, Qiyun Liang, Lufei Yuan, Lele Song, Rong Jiang, Jilu Shen
Frontiers in Microbiology.2026;[Epub] CrossRef - Reverse Transcription Loop-Mediated Isothermal Amplification Assay Using Samples Directly: Point-of-Care Detection of Severe Fever with Thrombocytopenia Syndrome Virus
Marla Anggita, Kyoko Hayashida, Miyuka Nishizato, Hiroshi Shimoda, Daisuke Hayasaka
Zoonotic Diseases.2025; 5(3): 19. CrossRef - One-pot one-step detection platform for severe fever with thrombocytopenia syndrome virus via the CRISPR/Cas12a detection system
Jiwei Shu, Qilong Tan, Zhe Huang, Tongjie Zhang, Ling Ye, Shuqin Fu, Zhilei Mao
Virology Journal.2025;[Epub] CrossRef - Portable RT-LAMP Platform for Rapid Point-of-Care Detection for SFTSV
Gi Chan Lee, Beom Kyu Kim, Sania Batool, Ji-Hyun Park, Seong Cheol Min, Ju Ryeong Lee, Dong Gyu Lee, Se Hee An, Aman Jain, Do Hyung Kim, Hui je Lee, Young Ki Choi, Min-Suk Song, Yun Hee Baek
Journal of Microbiology and Biotechnology.2025;[Epub] CrossRef - Long-Term Detection and Isolation of Severe Fever with Thrombocytopenia Syndrome (SFTS) Virus in Dog Urine
Yumiko Saga, Toshikazu Yoshida, Rieko Yoshida, Shunsuke Yazawa, Takahisa Shimada, Noriko Inasaki, Masae Itamochi, Emiko Yamazaki, Kazunori Oishi, Hideki Tani
Viruses.2023; 15(11): 2228. CrossRef - Advancements in the Worldwide Detection of Severe Fever with Thrombocytopenia Syndrome Virus Infection from 2009 to 2023
Lin Ai, Wei Wang, Zheng Teng
China CDC Weekly.2023; 5(31): 687. CrossRef - Molecular detection of “Candidatus Rickettsia tarasevichiae” by Loop-mediated Isothermal Amplification (LAMP) of the ompA gene
Jing Xue, Qing Ren, Rui Jian, Guang-Cheng Xie, Yongliang Chen, Jiangli Wang, Luanying Du, Wen-Ping Guo
Journal of Microbiological Methods.2022; 202: 106601. CrossRef - Dual-gene detection in a single-tube system based on CRISPR-Cas12a/Cas13a for severe fever thrombocytopenia syndrome virus
Yating Zhu, Chen Xing, Li Yang, Qian Li, Xiaofeng Wang, Jing Zhou, Cong Zhang, Cuiping Ren, Fahu Liu, Jun He, Bing Shen, Yinan Du, Yan Liu
Frontiers in Microbiology.2022;[Epub] CrossRef - CRISPR/Cas12a Technology Combined With RPA for Rapid and Portable SFTSV Detection
Mengqian Huang, Sihua Liu, Yanan Xu, Aqian Li, Wei Wu, Mifang Liang, Guoyu Niu, Zhiyun Wang, Tao Wang
Frontiers in Microbiology.2022;[Epub] CrossRef - Development of monoclonal antibody based IgG and IgM ELISA for diagnosis of severe fever with thrombocytopenia syndrome virus infection
Mei Zhang, Yanhua Du, Li Yang, Lin Zhan, Bin Yang, Xueyong Huang, Bianli Xu, Koichi Morita, Fuxun Yu
The Brazilian Journal of Infectious Diseases.2022; 26(4): 102386. CrossRef - Development of an RT-LAMP Assay for the Rapid Detection of SFTS Virus
Shiori Sano, Shuetsu Fukushi, Souichi Yamada, Shizuko Harada, Hitomi Kinoshita, Satoko Sugimoto, Tomoki Yoshikawa, Takeshi Kurosu, Yuki Takamatsu, Masayuki Shimojima, Shoichi Toda, Yuka Hamada, Naoki Fujisawa, Takayuki Sugimoto, Masayuki Saijo
Viruses.2021; 13(4): 693. CrossRef - A Real-Time Loop-Mediated Isothermal Amplification for Detection of the Wheat Dwarf Virus in Wheat and the Insect VectorPsammotettix alienus
Xingan Hao, Licheng Wang, Xudong Zhang, Qinrong Zhong, Jamal-U-Ddin Hajano, Liangsheng Xu, Yunfeng Wu
Plant Disease.2021; 105(12): 4113. CrossRef - Baseline mapping of severe fever with thrombocytopenia syndrome virology, epidemiology and vaccine research and development
Nathen E. Bopp, Jaclyn A. Kaiser, Ashley E. Strother, Alan D. T. Barrett, David W. C. Beasley, Virginia Benassi, Gregg N. Milligan, Marie-Pierre Preziosi, Lisa M. Reece
npj Vaccines.2020;[Epub] CrossRef
- Sequence analysis of the first B5 subgenogroup strain of enterovirus 71 isolated in Korea
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Yu Jung Won , Lae Hyung Kang , Ah Ra Lee , Bomina Paik , Hyun Kim , Sung Geun Lee , Seung Won Park , Seung Jin Hong , Soon Young Paik
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J. Microbiol. 2020;58(5):422-429. Published online March 28, 2020
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DOI: https://doi.org/10.1007/s12275-020-9539-z
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Abstract
PDF
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Enterovirus A71 (EV71), the main etiological agent of handfoot-
mouth disease (HFMD), circulates in many areas of the
world and has caused large epidemics since 1997, especially
in the Asia-Pacific region. In this study, we determined the
full-genome sequence of CMC718, a newly isolated EV71
strain in Korea. The CMC718 genome was 7,415 nucleotides
in length and was confirmed by whole-genome phylogenetic
analysis to belong to the B5 genotype. In particular, CMC718
demonstrated maximum identity with strain M988 of the B5
genotype and numerous amino acid variants were detected
in the 3D domain of the viral protein P3, which is consistent
with the mutation pattern of a B5 strain isolated in 2012–2013.
Comparison of the CMC718 sequence with other EV71 reference
strains confirmed the relationship and genetic variation
of CMC718. Our study was a full-genome sequence analysis
of the first EV71 strain of the B5 genotype isolated in
South Korea. This information will be a valuable reference
for the development of methods for the detection of recombinant
viruses, the tracking of infections, and the diagnosis
of EV71.
- Molecular genomic characterization of severe fever with thrombocytopenia syndrome virus isolates from South Korea
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Yu Jung Won , Lae Hyung Kang , Sung Geun Lee , Seung Won Park , Jae Ik Han , Soon Young Paik
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J. Microbiol. 2019;57(10):927-937. Published online August 3, 2019
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DOI: https://doi.org/10.1007/s12275-019-9174-8
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1,233
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Crossref
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Abstract
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Severe fever with thrombocytopenia syndrome (SFTS) is a
tick-borne emerging infectious disease caused by the SFTS
virus (SFTSV) and is a threat to public health due to its high
fatality rate. However, details on tick-to-human transmission
of SFTSV are limited. In this study, we determined the wholegenome
sequence of a South Korean SFTSV strain (CUKJJ01),
compared it to those of other recent human SFTSV
isolates, and identified the genetic variations and relationships
among the SFTSV strains. The genome of CUK-JJ01
was consistent with the genome of other members of the genus
Phlebovirus, including the large (L), medium (M), and
small (S) segments of 6368, 3378, and 1744 nucleotides, respectively.
Based on amino acid sequences of the M and S
segments, which are used to distinguish the six SFTSV genotypes,
CUK-JJ01 was classified as genotype B. Segment analysis
revealed that the L, M, and S segments were 97.49%,
97.18%, and 97.94% similar to those of KAJNH2/2013/
Korea, ZJZHSH-FDE/2012/China, and KADGH/2013/Korea,
respectively. Currently, only few studies on SFTSV have been
conducted in Korean population and most were limited to
serological analysis. Although the present study has limitations
in terms of number of sample analyzed, the findings
may serve as basis to understand the transmission and spread
of SFTSV, as well as for the development of diagnostic and
detection methods for viral recombinants by comparing
the whole genome sequence of SFTSV isolates from South
Korea and that of foreign isolates.
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Citations
Citations to this article as recorded by - Current status of severe fever with thrombocytopenia syndrome in China (Review)
Hao Sun, Quanman Hu, Saiwei Lu, Yanyan Yang, Li Zhang, Jinzhao Long, Yuefei Jin, Haiyan Yang, Shuaiyin Chen, Guangcai Duan
International Journal of Molecular Medicine.2025; 56(5): 1. CrossRef - The pathogen of SFTS: Bandavirus dabieense phylogenetic analysis and development of an online tool for genotyping
Hongxiao Wu, Tingting Liu, Yameng Mu, Ruihua Zhang, Jiale Yuan, Yanli Xu, Wenjing Niu, Yamin Sun, Wenchao Lin, Chuan Song, Wei Zhang, Ling Lin, Yuanyuan Zhang, Zhihai Chen
Acta Tropica.2025; 266: 107625. CrossRef - Whole-genome sequence analysis of SFTS bunyavirus in Huzhou, China
Deshun Xu, Lei Ji, Xiaofang Wu, Liping Chen, Paulo Lee Ho
PLOS ONE.2025; 20(2): e0318742. CrossRef - Circulation of tick-borne pathogens in wildlife of the Republic of Korea
Hye-ryung Byun, Seong-Ryeong Ji, Jun-Gu Kang, Chang-Yong Choi, Ki-Jeong Na, Jong-Taek Kim, Joon-Seok Chae
One Health.2024; 19: 100913. CrossRef - Molecular characterization of the genome of a new bunyavirus isolated in 2023 in Huzhou, China
Shili Song, Zhang Shen, Deshun Xu
Journal of Clinical Virology Plus.2024; 4(3): 100188. CrossRef - Multiple Genotypes and Reassortants of Severe Fever With Thrombocytopenia Syndrome Virus Co‐Circulating in Hangzhou in Southeastern China, 2013–2023
Yanping Wen, Zhimin Ni, Yan Hu, Jun Wu, Yezhen Fang, Guozhong Zhang, Renjie Huang, Shi Cheng, Feifei Cao, Qihao Xu, Yue Yu, Min Liu, Hongnv Yu, Liangliang Huo, Jun Li
Journal of Medical Virology.2024;[Epub] CrossRef - Genetic Diversity, Regional Distribution, and Clinical Characteristics of Severe Fever with Thrombocytopenia Syndrome Virus in Gangwon Province, Korea, a Highly Prevalent Region, 2019–2021
Mi-Young Moon, Hyeon Kyu Kim, Se-Jin Chung, Jae Hwan Byun, Ha-Na Kim, Woan Lee, Soon-Won Lee, Sezim Monoldorova, Sungkyeong Lee, Bo-Young Jeon, Eun-Joo Lim
Microorganisms.2023; 11(9): 2288. CrossRef - Identification of severe fever with thrombocytopenia syndrome virus genotypes in patients and ticks in Liaoning Province, China
Xiao-Hu Han, Yue Ma, Hong-Yan Liu, Dan Li, Yan Wang, Feng-Hua Jiang, Qing-Tian Gao, Feng Jiang, Bao-Shan Liu, Guo-Shun Shen, Ze-Liang Chen
Parasites & Vectors.2022;[Epub] CrossRef - Analysis of cross neutralizing activity of antibodies from sera of severe fever with thrombocytopenia syndrome patients to deal with different genotype strains
Jiaxin Xu, Yuanni Liu, Fan Zhang, Xin Wang, Weijin Huang, Yulong Wu, Boqing Li, Jiapeng Zhuang, Yixing Bing, Youchun Wang, Yuanyuan Qiao
Frontiers in Microbiology.2022;[Epub] CrossRef - Development of a real-time loop-mediated isothermal amplification method for the detection of severe fever with thrombocytopenia syndrome virus
Jae Woong Lee, Yu-Jung Won, Lae Hyung Kang, Sung-Geun Lee, Seung-Won Park, Soon-Young Paik
Journal of Microbiology.2020; 58(8): 711. CrossRef
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