Development of a real-time loop-mediated isothermal amplification method for the detection of severe fever with thrombocytopenia syndrome virus

Jae Woong Lee; Yu-Jung Won; Lae Hyung Kang; Sung-Geun Lee; Seung-Won Park; Soon-Young Paik

doi:10.1007/s12275-020-0109-1

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Development of a real-time loop-mediated isothermal amplification method for the detection of severe fever with thrombocytopenia syndrome virus: Jae Woong Lee ¹, Yu-Jung Won ¹, Lae Hyung Kang ¹, Sung-Geun Lee ², Seung-Won Park ³, Soon-Young Paik ¹; Journal of Microbiology 2020;58(8):711-715.
DOI: https://doi.org/10.1007/s12275-020-0109-1
Published online: May 18, 2020

¹Department of Microbiology, College of Medicine, The Catholic University of Korea, Seoul 06591, Republic of Korea, ²Korea Zoonosis Research Institute, Jeonbuk National University, Iksan 54596, Republic of Korea, ³Department of Biotechnology, Daegu Catholic University, Gyeongsan-si 38430, Republic of Korea

Corresponding author: Soon-Young Paik , Tel: +82-2-2258-7342;,

Received: 2 March 2020 • Revised: 14 April 2020 • Accepted: 16 April 2020

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Abstract

Severe fever with thrombocytopenia syndrome (SFTS) is being reported annually in South Korea since its first detection there in 2010. The causal agent is a negative-strand RNA virus 80–100 nm in diameter. It causes fever, thrombocytopenia, leukocytopenia, gastrointestinal symptoms, and neural symptoms. The mortality rate of SFTS was 32.6% among 172
case
s reported from 2012 to 2015 in South Korea. Thus, is necessary to develop an effective diagnostic method that selectively identifies the isolates circulating in South Korea. The real-time reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay is a simple, rapid, and sensitive approach for molecular diagnosis. Here, we designed novel primers for this assay and found that the technique had very high specificity, sensitivity, and efficiency. This real-time RTLAMP approach using the novel primers developed herein can be applied for early diagnosis of SFTSV strains in South Korea to reduce the mortality rate of SFTS.

Keywords: severe fever with thrombocytopenia syndrome (STFS), reverse transcription loop-mediated isothermal amplification (LAMP), molecular diagnostics, virus detection

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Development of a real-time loop-mediated isothermal amplification method for the detection of severe fever with thrombocytopenia syndrome virus

J. Microbiol. 2020;58(8):711-715. Published online May 18, 2020

DOI: https://doi.org/10.1007/s12275-020-0109-1

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