Journal of Microbiology

Kap-Hoon Han

6 Articles

The Observation of PlcA Mutation and Localization in Aspergillus nidulans: Chun-Seob Ahn , Young Taek Oh , Jeong-Geun Kim , Kap-Hoon Han , Chang-Won Lee , Jae Won Kim; J. Microbiol. 2014;52(7):590-596. Published online June 28, 2014; DOI: https://doi.org/10.1007/s12275-014-3651-x

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To know the function of the plcA gene, which encodes a putative phosphoinositide-specific phospholipase C, in a model filamentous fungus Aspergillus nidulans, it was disrupted thorough homologous recombination and examined. The germination rate of ΔplcA was reduced by approximately 65% and germination of ΔplcA at a lower temperature (25°C) was much slower than germination under normal conditions (37°C), suggesting the plcA is responsible for cold-sensitivity. The hyphal growth of ΔplcA was slightly reduced at 37°C and conspicuously reduced at 25°C. While germinating ΔplcA formed giant swollen spores, and generated short and thick hyphae. The results of the nuclear examination of ΔplcA showed nuclear division with missegregation, and the rate of nuclear division was lower than that of wild type at both 25°C and 37°C. The results of this study showed that plcA is localized to the nucleus through intracellular calcium signaling in A. nidulans. The abnormal nuclear division, resulting from plcA gene deletion, affects conidiation in asexual development. Taken together, these results suggested that plcA is required for normal vegetative growth, morphogenesis, conidiation, and nuclear division
in A. nidulans.

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Plant growth regulator diethyl aminoethyl hexanoate suppresses Aspergillus flavus growth and aflatoxin biosynthesis through disruption of redox homeostasis
Kai Ouyang, Lei He, Yarong Zhao, Siyi Tan, Guanxian Chen, Yingwen Wu, Xinyu Han, Xiaorong Wan, Qingqing Zhi, Gemma Reguera
Applied and Environmental Microbiology.2026;[Epub] CrossRef
Genome-Wide Mapping Reveals an Extensive AtfA Regulatory Influence on Development, Metabolism, and Stress Preparedness in Aspergillus nidulans
Márton Miskei, Sandugash Ibragimova, Beatrix Kocsis, Tibor Nagy, Hee-Soo Park, Tamás Emri, Jae-Hyuk Yu, Éva Leiter, István Pócsi
Cells.2025; 14(24): 1965. CrossRef
Regulators of the Asexual Life Cycle of Aspergillus nidulans
Ye-Eun Son, Jae-Hyuk Yu, Hee-Soo Park
Cells.2023; 12(11): 1544. CrossRef
The Kinetochore Protein Spc105, a Novel Interaction Partner of LaeA, Regulates Development and Secondary Metabolism in Aspergillus flavus
Qing-Qing Zhi, Lei He, Jie-Ying Li, Jing Li, Zhen-Long Wang, Guang-Yao He, Zhu-Mei He
Frontiers in Microbiology.2019;[Epub] CrossRef

A Putative APSES Transcription Factor Is Necessary for Normal Growth and Development of Aspergillus nidulans: Ji-Yeon Lee , Lee-Han Kim , Ha-Eun Kim , Jae-Sin Park , Kap-Hoon Han , Dong-Min Han; J. Microbiol. 2013;51(6):800-806. Published online December 19, 2013; DOI: https://doi.org/10.1007/s12275-013-3100-2

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Abstract PDF

The nsdD gene encoding a GATA type transcription factor positively controls sexual development in Aspergillus nidulans. According to microarray data, 20 genes that were upregulated by deleting nsdD during various life cycle stages were randomly selected and deleted for functional analysis. None of the mutants showed apparent changes in growth or development compared with those of the wild-type except the AN3154 gene that encodes a putative APSES transcription factor and is an ortholog of Saccharomyces cerevisiae swi4. Deleting AN3154 resulted in retarded growth and development, and the gene was named rgdA (retared growth and development). The rgdA deletion mutant developed a reduced number of conidia even under favorable conditions for asexual development. The retarded growth and development was partially suppressed by the veA1 mutation. The conidial heads of the mutant aborted, showing reduced and irregular shaped phialides. Fruiting body development was delayed compared with that in the wild-type. The mutant did not respond to various nutritional or environmental factors that affected the development patterns. The rgdA gene was expressed at low levels throughout the life cycle and was not significantly affected by several regulators of sexual and asexual development such as nsdD, veA, stuA, or brlA. However, the rgdA gene affected brlA and abaA expression, which function as key regulators of asexual sporulation, suggesting that rgdA functions upstream of those genes.

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AoMbp1 interacts with AoSwi6 and regulates hyphal development, stress tolerance, trap morphogenesis, and pathogenicity in Arthrobotrys oligospora
Na Bai, Hui Yuan, Guiqiu Luo, Yi Chen, Meihua Xie, Jinkui Yang
Pesticide Biochemistry and Physiology.2026; 218: 106930. CrossRef
The analysis of transcriptomics reveals the function of TrPLD3 in the pathogenicity of Trichothecium roseum infecting apples
Xiao Li, Qianqian Zhang, Qili Liu, Xiaobin Xu, Jinzhu Li, Dandan Zhu, Yuanyuan Zong, Huali Xue, Yang Bi
Postharvest Biology and Technology.2025; 227: 113628. CrossRef
Genome-Wide Mapping Reveals an Extensive AtfA Regulatory Influence on Development, Metabolism, and Stress Preparedness in Aspergillus nidulans
Márton Miskei, Sandugash Ibragimova, Beatrix Kocsis, Tibor Nagy, Hee-Soo Park, Tamás Emri, Jae-Hyuk Yu, Éva Leiter, István Pócsi
Cells.2025; 14(24): 1965. CrossRef
Putative APSES family transcription factor mbp1 plays an essential role in regulating cell wall synthesis in the agaricomycete Pleurotus ostreatus
Hayase Kojima, Moriyuki Kawauchi, Yuitsu Otsuka, Kim Schiphof, Kenya Tsuji, Akira Yoshimi, Chihiro Tanaka, Shigekazu Yano, Takehito Nakazawa, Yoichi Honda
Fungal Genetics and Biology.2024; 175: 103936. CrossRef
Regulators of the Asexual Life Cycle of Aspergillus nidulans
Ye-Eun Son, Jae-Hyuk Yu, Hee-Soo Park
Cells.2023; 12(11): 1544. CrossRef
Characterization of the mbsA Gene Encoding a Putative APSES Transcription Factor in Aspergillus fumigatus
Yong-Ho Choi, Sang-Cheol Jun, Min-Woo Lee, Jae-Hyuk Yu, Kwang-Soo Shin
International Journal of Molecular Sciences.2021; 22(7): 3777. CrossRef
The Putative APSES Transcription Factor RgdA Governs Growth, Development, Toxigenesis, and Virulence in Aspergillus fumigatus
Sang-Cheol Jun, Yong-Ho Choi, Min-Woo Lee, Jae-Hyuk Yu, Kwang-Soo Shin, Aaron P. Mitchell
mSphere.2020;[Epub] CrossRef
Analogous and Diverse Functions of APSES-Type Transcription Factors in the Morphogenesis of the Entomopathogenic Fungus Metarhizium rileyi
Caiyan Xin, Jinping Zhang, Siji Nian, Guangxi Wang, Zhongkang Wang, Zhangyong Song, Guangwei Ren, Ning-Yi Zhou
Applied and Environmental Microbiology.2020;[Epub] CrossRef
Distribution, evolution and expression ofGATA-TFsprovide new insights into their functions in light response and fruiting body development ofTolypocladium guangdongense
Chenghua Zhang, Gangzheng Wang, Wangqiu Deng, Taihui Li
PeerJ.2020; 8: e9784. CrossRef
Characterization of the APSES-family transcriptional regulators of Histoplasma capsulatum
Larissa V G Longo, Stephanie C Ray, Rosana Puccia, Chad A Rappleye
FEMS Yeast Research.2018;[Epub] CrossRef
Essential APSES Transcription Factors for Mycotoxin Synthesis, Fungal Development, and Pathogenicity in Aspergillus flavus
Guangshan Yao, Feng Zhang, Xinyi Nie, Xiuna Wang, Jun Yuan, Zhenhong Zhuang, Shihua Wang
Frontiers in Microbiology.2017;[Epub] CrossRef
Characterizing the nuclear proteome of Paracoccidioides spp.
Lucas Nojosa Oliveira, Luciana Casaletti, Sônia Nair Báo, Clayton Luiz Borges, Patrícia de Sousa Lima, Célia Maria de Almeida Soares
Fungal Biology.2016; 120(10): 1209. CrossRef
Isolation and Characterization of Two Methyltransferase Genes, AfuvipB and AfuvipC in Aspergillus fumigatus
Mohammed A. Abdo Elgabbar, Kap-Hoon Han
The Korean Journal of Mycology.2015; 43(1): 33. CrossRef
Depletion of ε-COP in the COPI Vesicular Coat Reduces Cleistothecium Production inAspergillus nidulans
Eun-Hye Kang, Eun-Jung Song, Jun Ho Kook, Hwan-Hee Lee, Bo-Ri Jeong, Hee-Moon Park
Mycobiology.2015; 43(1): 31. CrossRef
FgFlbD regulates hyphal differentiation required for sexual and asexual reproduction in the ascomycete fungus Fusarium graminearum
Hokyoung Son, Myung-Gu Kim, Suhn-Kee Chae, Yin-Won Lee
Journal of Microbiology.2014; 52(11): 930. CrossRef
Transcriptional regulation of fksA, a β-1,3-glucan synthase gene, by the APSES protein StuA during Aspergillus nidulans development
Bum-Chan Park, Yun-Hee Park, Soohyun Yi, Yu Kyung Choi, Eun-Hye Kang, Hee-Moon Park
Journal of Microbiology.2014; 52(11): 940. CrossRef

Isolation and Characterization of Self-Fertile Suppressors from the Sterile nsdD Deletion Mutant of Aspergillus nidulans: Dong-Beom Lee , Lee Han Kim , Jin-Pyo Kim , Kap-Hoon Han , Dong-Min Han; J. Microbiol. 2011;49(6):1054-1057. Published online December 28, 2011; DOI: https://doi.org/10.1007/s12275-011-1111-4

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Abstract PDF

To identify downstream and/or interactive factors of the nsdD gene, which encodes a positive regulator of sexual development of Aspergillus nidulans, suppressor mutants displaying a self-fertile phenotype were isolated from a sterile nsdD deletion mutant. At least five different loci (sndA-E) were identified and genetically analyzed. In the nsdD+ background, most of the suppressors showed a marked increment of sexual development, even under the stress conditions that normally inhibited sexual development. The common phenotype of the suppressor mutants suggested the involvement of the snd genes in the negative regulation of sexual development in response to the environmental factors.

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Inactivation of the global regulator LaeA in Monascus ruber results in a species-dependent response in sporulation and secondary metabolism
Qingpei Liu, Li Cai, Yanchun Shao, Youxiang Zhou, Mu Li, Xiaohong Wang, Fusheng Chen
Fungal Biology.2016; 120(3): 297. CrossRef
A putative APSES transcription factor is necessary for normal growth and development of Aspergillus nidulans
Ji-Yeon Lee, Lee-Han Kim, Ha-Eun Kim, Jae-Sin Park, Kap-Hoon Han, Dong-Min Han
Journal of Microbiology.2013; 51(6): 800. CrossRef
Isolation and Characterization of the gtfA Gene Encoding GAL4-Like Transcription Factor in Aspergillus nidulans
Jae-Sin Park, Dong-Min Han
The Korean Journal of Microbiology.2013; 49(1): 8. CrossRef

Simple Identification of veA1 Mutation in Aspergillus nidulans: Kap-Hoon Han , Jae-Sin Park , Keon Sang Chae , Dong-Min Han; J. Microbiol. 2010;48(6):885-887. Published online January 9, 2011; DOI: https://doi.org/10.1007/s12275-010-0506-y

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The veA gene plays an important role in development of a homothallic filamentous fungus Aspergillus nidulans. The veA1 phenotype can be difficult to distinguish from the wild-type veA. Despite the importance of the veA allele, no efficient identification method has been reported besides DNA sequencing. Here, we present simple physiological and molecular biological ways to distinguish between the veA wild-type and veA1 allele. The novel approaches, which involve incubation in the presence of oxalic acid, polymerase chain reaction using double mismatched primers, and BstXI enzyme digestion, are simpler, faster and more cost-efficient than genome sequencing.

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Chemical composition of essential oil and antifungal activity of Artemisia persica Boiss. from Iran
Reza Dehghani Bidgoli
Journal of Food Science and Technology.2021; 58(4): 1313. CrossRef
Survival factor SvfA plays multiple roles in differentiation and is essential for completion of sexual development in Aspergillus nidulans
Joo-Yeon Lim, Eun-Hye Kang, Yun-Hee Park, Jun-Ho Kook, Hee-Moon Park
Scientific Reports.2020;[Epub] CrossRef
Expression of sexual genes in Aspergillus fumigatus homogeneous culture produced by vegetative mass mating
Joo-Yeon Lim, Hee-Moon Park
Journal of Microbiology.2019; 57(8): 688. CrossRef
HMGB proteins are required for sexual development in Aspergillus nidulans
Eszter Bokor, Judit Ámon, Kabichandra Keisham, Zoltán Karácsony, Csaba Vágvölgyi, Zsuzsanna Hamari, Kap-Hoon Han
PLOS ONE.2019; 14(4): e0216094. CrossRef
NapA Mediates a Redox Regulation of the Antioxidant Response, Carbon Utilization and Development in Aspergillus nidulans
Ariann E. Mendoza-Martínez, Fernando Lara-Rojas, Olivia Sánchez, Jesús Aguirre
Frontiers in Microbiology.2017;[Epub] CrossRef
Growth-Phase Sterigmatocystin Formation on Lactose Is Mediated via Low Specific Growth Rates in Aspergillus nidulans
Zoltán Németh, Ákos Molnár, Balázs Fejes, Levente Novák, Levente Karaffa, Nancy Keller, Erzsébet Fekete
Toxins.2016; 8(12): 354. CrossRef
The SrkA Kinase Is Part of the SakA Mitogen-Activated Protein Kinase Interactome and Regulates Stress Responses and Development in Aspergillus nidulans
Rafael Jaimes-Arroyo, Fernando Lara-Rojas, Özgür Bayram, Oliver Valerius, Gerhard H. Braus, Jesús Aguirre
Eukaryotic Cell.2015; 14(5): 495. CrossRef
A putative APSES transcription factor is necessary for normal growth and development of Aspergillus nidulans
Ji-Yeon Lee, Lee-Han Kim, Ha-Eun Kim, Jae-Sin Park, Kap-Hoon Han, Dong-Min Han
Journal of Microbiology.2013; 51(6): 800. CrossRef
FlbD, a Myb Transcription Factor of Aspergillus nidulans, Is Uniquely Involved in both Asexual and Sexual Differentiation
Jenny Arratia-Quijada, Olivia Sánchez, Claudio Scazzocchio, Jesús Aguirre
Eukaryotic Cell.2012; 11(9): 1132. CrossRef

Screening of Growth- or Development-related Genes by Using Genomic Library with Inducible Promoter in Aspergillus nidulans: Bang-Yong Lee , Sang-Yong Han , Han Gil Choi , Jee Hyun Kim , Kap-Hoon Han , Dong-Min Han; J. Microbiol. 2005;43(6):523-528.; DOI: https://doi.org/2295 [pii]

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Abstract PDF: Using the genomic library constructed at the downstream of the niiA promoter, which induces the over-expression of an inserted DNA fragment, we have attempted to screen the genes affecting growth or development by over-expression. The wild-type strain was transformed using the AMA-niiA(p) library and cultured on 1.2 M sorbitol media, in which asexual sporulation is induced, but sexual development is repressed. Over 100,000 strains transformed to pyrG+ were analyzed with regard to any changes in phenotype. Consequently, seven strains were isolated for further analyses. These strains were designated NOT [niiA(p) over-expression transformants] stains. Four of the strains were of the inducible type, and the remaining strains were of the multi-copy suppression type. Two of the inducible-type strains, NOT1 and NOT40, harbored genes which had been inserted in reverse direction, suggesting that the mutant phenotypes had been derived from an excess amount of anti-sense mRNA. Domain analyses of the deduced polypeptides from the DNA fragments rescued from the transformants revealed that NOT1, NOT40 and NOT6 harbored a LisH motif, a forkhead domain, and a Zn(II)2Cys6 binuclear zinc cluster, respectively.

Environmental factors affecting development of Aspergillus nidulans: Kap-Hoon Han , Dong-Beom Lee , Jong-Hak Kim , Min-Su Kim , Kyu-Yong Han , Won-Shin Kim , Young-Soon Park , Heui-Baik Kim^ , Dong-Min Han^; J. Microbiol. 2003;41(1):34-40.

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Abstract PDF: Aspergillus nidulans, a homothalic ascomycete, has a complete sexual reproductive cycle as well as an asexual one. Both sexual and asexual development are known to be genetically programmed, but are also strongly affected by environmental factors including nutrients, light, temperature and osmolarity. We have examined these factors to define favored conditions for fruiting body (cleistothecium) formation. In general, fruiting body formation was enhanced where carbon and nitrogen sources were sufficient. Limitation of C-source caused predominant asexual development while inhibiting sexual development. When higher concentrations of glucose were supplied, more cleistothecia were formed. Other carbon sources including lactose, galactose and glycerol made the fungus develop cleistothecia very well, whereas acetate caused asexual sporulation only. Organic nitrogen sources like casein hydrolysate and glycine, and an increase in nitrate or ammonium concentration also enhanced sexual development. In addition to nutrient effects, low levels of aerobic respiration, caused either by platesealing or treatment with various chemicals, favored sexual development. Carbon limitation, light exposure and a high concentration of salts promoted asexual development preferentially, suggesting that stress conditions may drive the cell to develop asexual sporulation while comfortable and wellnourished growth conditions favored sexual development.

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