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Research Support, Non-U.S. Gov't
- NOTE] Simple Identification of veA1 Mutation in Aspergillus nidulans
- Kap-Hoon Han 1, Jae-Sin Park 2, Keon Sang Chae 3, Dong-Min Han 2,4
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Journal of Microbiology 2010;48(6):885-887.
DOI: https://doi.org/10.1007/s12275-010-0506-y
Published online: January 9, 2011
1Department of Pharmaceutical Engineering, Woosuk University, Samrye 565-701, Republic of Korea, 2Division of Life Science Wonkwang University, Iksan 570-749, Republic of Korea, 3Division of Biological Science, Jeonbuk University, Jeonju 561-756, Republic of Korea, 4Institute of Biotechnology, Wonkwang University, Iksan 570-749, Republic of Korea
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Corresponding author:
Dong-Min Han , Tel: +82-63-850-6220,
Received: 8 December 2010 • Accepted: 16 December 2010
Abstract
The veA gene plays an important role in development of a homothallic filamentous fungus Aspergillus nidulans. The veA1 phenotype can be difficult to distinguish from the wild-type veA. Despite the importance of the veA allele, no efficient identification method has been reported besides DNA sequencing. Here, we present simple physiological and molecular biological ways to distinguish between the veA wild-type and veA1 allele. The novel approaches, which involve incubation in the presence of oxalic acid, polymerase chain reaction using double mismatched primers, and BstXI enzyme digestion, are simpler, faster and more cost-efficient than genome sequencing.
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