Journal of Microbiology

Joao Atilio Jorge

3 Articles

Biochemical Properties of an Extracellular Trehalase from Malbranchea pulchella var. Sulfurea: Marita Gimenez Pereira , Luis Henrique Souza Guimarães , Rosa Prazeres Melo Furriel , Maria de Lourdes , Teixeira de Moraes Polizeli , Hector Francisco Terenzi , João Atílio Jorge; J. Microbiol. 2011;49(5):809-815. Published online November 9, 2011; DOI: https://doi.org/10.1007/s12275-011-0532-4

708 View
0 Download
9 Crossref

The thermophilic fungus Malbranchea pulchella var. sulfurea produced good amounts of extracellular trehalase activity when grown for long periods on starch, maltose or glucose as the main carbon source. Studies with young cultures suggested that the main role of the extracellular acid trehalase is utilizing trehalose as a carbon source. The specific activity of the purified enzyme in the presence of manganese (680 U/mg protein) was comparable to that of other thermophilic fungi enzymes, but many times higher than the values reported for trehalases from other microbial sources. The apparent molecular mass of the native enzyme was estimated to be 104 kDa by gel filtration and 52 kDa by SDS-PAGE, suggesting that the enzyme was composed by two subunits. The carbohydrate content of the purified enzyme was estimated to be 19% and the pI was 3.5. The optimum pH and temperature were 5.0-5.5 and 55°C, respectively. The purified enzyme was stimulated by manganese and inhibited by calcium ions, and insensitive to ATP and ADP, and 1 mM silver ions. The apparent KM values for trehalose hydrolysis by the purified enzyme in the absence and presence of manganese chloride were 2.70±0.29 and 2.58±0.13 mM, respectively. Manganese ions affected only the apparent Vmax, increasing the catalytic efficiency value by 9.2-fold. The results reported herein indicate that Malbranchea pulchella produces a trehalase with mixed biochemical properties, different from the conventional acid and neutral enzymes and also from trehalases from other thermophilic fungi.

Citations

Citations to this article as recorded by

Efficient production and characterization of a newly identified trehalase for inhibiting the formation of bacterial biofilms
Chang Su, Jin-Song Gong, Qi Dong, Nan-Kai Wang, Heng Li, Jin-Song Shi, Zheng-Hong Xu
International Journal of Biological Macromolecules.2024; 262: 129928. CrossRef
Genomic Sequencing and Functional Analysis of the Ex-Type Strain of Malbranchea zuffiana
Alan Omar Granados-Casas, Ana Fernández-Bravo, Alberto Miguel Stchigel, José Francisco Cano-Lira
Journal of Fungi.2024; 10(9): 600. CrossRef
Increased Malbranchea pulchella β-glucosidase production and its application in agroindustrial residue hydrolysis: A research based on experimental designs
Lummy Maria Oliveira Monteiro, Ana Claudia Vici, Josana Maria Messias, Paulo Ricardo Heinen, Vanessa Elisa Pinheiro, Carem Gledes Vargas Rechia, Marcos S. Buckeridge, Maria de Lourdes Teixeira de Moraes Polizeli
Biotechnology Reports.2021; 30: e00618. CrossRef
A Highly Glucose Tolerant ß-Glucosidase from Malbranchea pulchella (MpBg3) Enables Cellulose Saccharification
Lummy Maria Oliveira Monteiro, Ana Claudia Vici, Matheus Pinto Pinheiro, Paulo Ricardo Heinen, Arthur Henrique Cavalcante de Oliveira, Richard John Ward, Rolf Alexander Prade, Marcos S. Buckeridge, Maria de Lourdes Teixeira de Moraes Polizeli
Scientific Reports.2020;[Epub] CrossRef
Evolutionary and structure-function analysis elucidates diversification of prokaryotic and eukaryotic trehalases
Meenakshi B. Tellis, Nidhi N. Gujar, Rakesh S. Joshi
Journal of Biomolecular Structure and Dynamics.2019; 37(11): 2926. CrossRef
Thermophilic molds: Biology and applications
Bijender Singh, Marcio J. Poças-Fonseca, B. N. Johri, Tulasi Satyanarayana
Critical Reviews in Microbiology.2016; 42(6): 985. CrossRef
A trehalase from Zunongwangia sp.: characterization and improving catalytic efficiency by directed evolution
Qipeng Cheng, Haofeng Gao, Nan Hu
BMC Biotechnology.2016;[Epub] CrossRef
A novel thermostable xylanase GH10 from Malbranchea pulchella expressed in Aspergillus nidulans with potential applications in biotechnology
Liliane FC Ribeiro, Rosymar C De Lucas, Gabriela L Vitcosque, Lucas F Ribeiro, Richard J Ward, Marcelo V Rubio, Andre RL Damásio, Fabio M Squina, Rebecca C Gregory, Paul H Walton, João A Jorge, Rolf A Prade, Marcos S Buckeridge, Maria de Lourdes TM Polize
Biotechnology for Biofuels.2014;[Epub] CrossRef
Trehalases: A neglected carbon metabolism regulator?
Aarón Barraza, Federico Sánchez
Plant Signaling & Behavior.2013; 8(7): e24778. CrossRef

Purification and Biochemical Properties of a Glucose-Stimulated β-D-Glucosidase Produced by Humicola grisea var. thermoidea Grown on Sugarcane Bagasse: Cesar Vanderlei Nascimento , Flávio Henrique Moreira Souza , Douglas Chodi Masui , Francisco Assis Leone , Rosane Marina Peralta , João Atílio Jorge , Rosa Prazeres Melo Furriel; J. Microbiol. 2010;48(1):53-62. Published online March 11, 2010; DOI: https://doi.org/10.1007/s12275-009-0159-x

733 View
0 Download
60 Crossref

Abstract PDF

The effect of several carbon sources on the production of mycelial-bound β-glucosidase by Humicola grisea var. thermoidea in submerged fermentation was investigated. Maximum production occurred when cellulose was present in the culture medium, but higher specific activities were achieved with cellobiose or sugarcane bagasse. Xylose or glucose (1%) in the reaction medium stimulated β-glucosidase activity by about 2-fold in crude extracts from mycelia grown in sugarcane bagasse. The enzyme was purified by ammonium sulfate precipitation, followed by Sephadex G-200 and DEAE-cellulose chromatography, showing a single band in PAGE and SDS-PAGE. The β-glucosidase had a carbohydrate content of 43% and showed apparent molecular masses of 57 and 60 kDa, as estimated by SDS-PAGE and gel filtration, respectively. The optimal pH and temperature were 6.0 and 50°C, respectively. The purified enzyme was thermostable up to 60 min in water at 55°C and showed half-lives of 7 and 14 min when incubated in the absence or presence of 50 mM glucose, respectively, at 60°C. The enzyme hydrolyzed p-nitrophenyl-β-D-glucopyranoside, p-nitrophenyl-β-galactopyranoside, p-nitrophenyl-β-D-fucopyranoside, p-nitrophenyl-β-D-xylopyranoside, o-nitrophenyl-β-Dgalactopyranoside, lactose, and cellobiose. The best synthetic and natural substrates were p-nitrophenyl-β-Dfucopyranoside and cellobiose, respectively. Purified enzyme activity was stimulated up to 2-fold by glucose or xylose at concentrations from 25 to 200 mM. The addition of purified or crude β-glucosidase to a reaction medium containing Trichoderma reesei cellulases increased the saccharification of sugarcane bagasse by about 50%. These findings suggest that H. grisea var. thermoidea β-glucosidase has a potential for biotechnological applications in the bioconversion of lignocellulosic materials.

Citations

Citations to this article as recorded by

Biotechnological Potential of a Glucose-Tolerant β-Glucosidase from Rasamsonia composticola (β-GluRc) in Sugarcane Bagasse Saccharification
Isabela Pavão Vargas, Rodrigo Mattos Silva Galeano, Aline Pereira de Almeida, Ana Lorena de Oliveira Simas, João Victor Souza Ribeiro, Maria de Lourdes Teixeira de Moraes Polizeli, Douglas Chodi Masui, Giovana Cristina Giannesi, Roberto Ruller, Fabiana Fo
Waste and Biomass Valorization.2026; 17(8): 4723. CrossRef
Xylose and ethanol stimulate a thermostable β-glucosidase from Humicola brevis var. thermoidea
Luciane Gomes Freire, Aline Pereira de Almeida, Felipe Araújo Nascimento, Camila Langer Marciano, Fabiana Fonseca Zanoelo, Giovana Cristina Giannesi, José Carlos Santos dos Salgado, Maria de Lourdes Teixeira Moraes Polizeli, Roberto Ruller, Douglas Chodi
Brazilian Journal of Microbiology.2026;[Epub] CrossRef
Belowground Interaction in Tea/Soybean Intercropping Enhances Tea Quality by Improving Soil Nutrient Dynamics
Tianqi Wang, Xiaoyu Mu, Erdong Ni, Qinwen Wang, Shuyue Li, Jingying Mao, Dandan Qing, Bo Li, Yuan Chen, Wenjie Chen, Cuiyue Liang, Hualing Wu, Xing Lu, Jiang Tian
Plants.2025; 14(11): 1691. CrossRef
Computational Insights into Glucose Tolerance and Stimulation in a Family 1 β-glucosidase
Artur H. S. Dias, Munir S. Skaf, Rodrigo L. Silveira
Journal of Chemical Information and Modeling.2025; 65(13): 7102. CrossRef
Glucose binding to the gatekeeper region induces the glucose stimulation of β-glucosidase Cba3 from Cellulomonas biazotea
Dongeon Kim, Ki Hyun Nam, In Jung Kim
International Journal of Biological Macromolecules.2025; 319: 145590. CrossRef
Moderately thermostable GH1 β-glucosidases from hyperacidophilic archaeon Cuniculiplasma divulgatum S5
Anna N Khusnutdinova, Hai Tran, Saloni Devlekar, Marco A Distaso, Ilya V Kublanov, Tatiana Skarina, Peter Stogios, Alexei Savchenko, Manuel Ferrer, Olga V Golyshina, Alexander F Yakunin, Peter N Golyshin
FEMS Microbiology Ecology.2024;[Epub] CrossRef
Parasitic dodder expresses an arsenal of secreted cellulases with multi-substrate specificity during host invasion
Hilary Edema, Thomas Bawin, Stian Olsen, Kirsten Krause, Katja Karppinen
Plant Physiology and Biochemistry.2024; 210: 108633. CrossRef
Recombinant GH3 β-glucosidase stimulated by xylose and tolerant to furfural and 5-hydroxymethylfurfural obtained from Aspergillus nidulans
Diandra de Andrades, Robson C. Alnoch, Gabriela S. Alves, Jose C. S. Salgado, Paula Z. Almeida, Gabriela Leila Berto, Fernando Segato, Richard J. Ward, Marcos S. Buckeridge, Maria de Lourdes T. M. Polizeli
Bioresources and Bioprocessing.2024;[Epub] CrossRef
Characterization of a novel end product tolerant and thermostable cellulase from Neobacillus sedimentimangrovi UE25
Uroosa Ejaz, Syed Faraz Moin, Muhammad Sohail, Gaber A.M. Mersal, Mohamed M. Ibrahim, Salah M. El-Bahy
Enzyme and Microbial Technology.2023; 162: 110133. CrossRef
Improving the cellobiose hydrolysis activity of glucose-stimulating β-glucosidase Bgl2A
Shuaifeng Liu, Meng Zhang, Dengwang Hong, Zemin Fang, Yazhong Xiao, Wei Fang, Xuecheng Zhang
Enzyme and Microbial Technology.2023; 169: 110289. CrossRef
Characterization of a New Glucose-Tolerant GH1 β-Glycosidase from Aspergillus fumigatus with Transglycosylation Activity
Lucas Matheus Soares Pereira, Aline Vianna Bernardi, Luis Eduardo Gerolamo, Wellington Ramos Pedersoli, Cláudia Batista Carraro, Roberto do Nascimento Silva, Sergio Akira Uyemura, Taísa Magnani Dinamarco
International Journal of Molecular Sciences.2023; 24(5): 4489. CrossRef
Biological denitrification potential of cellulase-producing Cupriavidus sp. ZY7 and denitrifying Aquabacterium sp. XL4 at low carbon-to-nitrogen ratio: Performance and synergistic properties
Zhenyu Zhai, Junfeng Su, Amjad Ali, Liang Xu, Fazli Wahid
Bioresource Technology.2022; 360: 127600. CrossRef
Rhamnolipid enhanced beta-glucosidase from Paenibacillus sp. LLZ1 for in situ lignocellulose saccharification in ionic liquids
Xuemei Yao, Lei Xiao, Hang Liu, Min Zhou, Zheng Zhou, Xin Ju, Liangzhi Li
Biomass Conversion and Biorefinery.2022; 12(11): 5011. CrossRef
Biorefinery Gets Hot: Thermophilic Enzymes and Microorganisms for Second-Generation Bioethanol Production
Luca Zuliani, Annabel Serpico, Mario De Simone, Nicola Frison, Salvatore Fusco
Processes.2021; 9(9): 1583. CrossRef
Increased Malbranchea pulchella β-glucosidase production and its application in agroindustrial residue hydrolysis: A research based on experimental designs
Lummy Maria Oliveira Monteiro, Ana Claudia Vici, Josana Maria Messias, Paulo Ricardo Heinen, Vanessa Elisa Pinheiro, Carem Gledes Vargas Rechia, Marcos S. Buckeridge, Maria de Lourdes Teixeira de Moraes Polizeli
Biotechnology Reports.2021; 30: e00618. CrossRef
A new and unusual β-glucosidase from Aspergillus fumigatus: Catalytic activity at high temperatures and glucose tolerance
Aline Ratuchne, Adriana Knob
Biocatalysis and Agricultural Biotechnology.2021; 35: 102064. CrossRef
Characterization of a glucose-stimulated β-glucosidase from Microbulbifer sp. ALW1
Zedong Jiang, Liufei Long, Meifang Liang, Hebin Li, Yanhong Chen, Mingjing Zheng, Hui Ni, Qingbiao Li, Yanbing Zhu
Microbiological Research.2021; 251: 126840. CrossRef
Investigation of toxicity attenuation mechanism of tetrahydroxy stilbene glucoside in Polygonum multiflorum Thunb. by Ganoderma lucidum
Yan Lin, Xi Peng, Bohou Xia, Zhimin Zhang, Chun Li, Ping Wu, Limei Lin, Duanfang Liao
Journal of Ethnopharmacology.2021; 280: 114421. CrossRef
Unconventional β-Glucosidases: A Promising Biocatalyst for Industrial Biotechnology
Ravish Godse, Hemangi Bawane, Jyoti Tripathi, Ram Kulkarni
Applied Biochemistry and Biotechnology.2021; 193(9): 2993. CrossRef
β-Glucosidase genes differentially expressed during composting
Xinyue Zhang, Bo Ma, Jiawen Liu, Xiehui Chen, Shanshan Li, Erlie Su, Liyuan Gao, Hongtao Li
Biotechnology for Biofuels.2020;[Epub] CrossRef
Effect of aeration rates on enzymatic activity and bacterial community succession during cattle manure composting
Mianshen Ge, Haibin Zhou, Yujun Shen, Haibo Meng, Ran Li, Jun Zhou, Hongsheng Cheng, Xi Zhang, Jingtao Ding, Jian Wang, Jiarui Wang
Bioresource Technology.2020; 304: 122928. CrossRef
An unusual GH1 β-glucosidase from marine sediment with β-galactosidase and transglycosidation activities for superior galacto-oligosaccharide synthesis
Pengjun Deng, Chunyu Meng, Yan Wu, Jie Xu, Xiaoqing Tang, Xuecheng Zhang, Yazhong Xiao, Xiaotang Wang, Zemin Fang, Wei Fang
Applied Microbiology and Biotechnology.2020; 104(11): 4927. CrossRef
Understanding the key regulatory functions of red mud in cellulose breakdown and succession of β-glucosidase microbial community during composting
Zhiwei Jiang, Qingran Meng, Qiuqi Niu, Susu Wang, Hailong Yan, Qunliang Li
Bioresource Technology.2020; 318: 124265. CrossRef
Improving the cellobiose-hydrolysis activity and glucose-tolerance of a thermostable β-glucosidase through rational design
Xiwen Liu, Lichuang Cao, Jianhua Zeng, Yuhuan Liu, Wei Xie
International Journal of Biological Macromolecules.2019; 136: 1052. CrossRef
Insect gut as a bioresource for potential enzymes - an unexploited area for industrial biotechnology
M. Kannan, D. Mubarakali, B. Thiyonila, M. Krishnan, B. Padmanaban, S. Shantkriti
Biocatalysis and Agricultural Biotechnology.2019; 18: 101010. CrossRef
Probing the Effect of Glucose on the Activity and Stability of β-Glucosidase: An All-Atom Molecular Dynamics Simulation Investigation
Sukanya Konar, Sushant K. Sinha, Supratim Datta, Pradip Kr. Ghorai
ACS Omega.2019; 4(6): 11189. CrossRef
Understanding the glucose tolerance of an archaeon β-glucosidase from Thermococcus sp.
Sushant K. Sinha, K. Prakash Reddy, Supratim Datta
Carbohydrate Research.2019; 486: 107835. CrossRef
Temperature sensitivity of mineral-enzyme interactions on the hydrolysis of cellobiose and indican by β-glucosidase
Ziming Yang, Yiju Liao, Xuan Fu, Jared Zaporski, Stephanie Peters, Megan Jamison, Yurong Liu, Stan D. Wullschleger, David E. Graham, Baohua Gu
Science of The Total Environment.2019; 686: 1194. CrossRef
Immobilization of a β-glucosidase and an endoglucanase in ferromagnetic nanoparticles: A study of synergistic effects
Sibeli Carli, Lara Aparecida Buffoni de Campos Carneiro, Richard John Ward, Luana Parras Meleiro
Protein Expression and Purification.2019; 160: 28. CrossRef
Microbial Beta Glucosidase Enzymes: Recent Advances in Biomass Conversation for Biofuels Application
Neha Srivastava, Rishabh Rathour, Sonam Jha, Karan Pandey, Manish Srivastava, Vijay Kumar Thakur, Rakesh Singh Sengar, Vijai K. Gupta, Pranab Behari Mazumder, Ahamad Faiz Khan, Pradeep Kumar Mishra
Biomolecules.2019; 9(6): 220. CrossRef
Characterization of cold adapted and ethanol tolerant β-glucosidase from Bacillus cellulosilyticus and its application for directed hydrolysis of cellobiose to ethanol
Jian Wu, Alei Geng, Rongrong Xie, Hongcheng Wang, Jianzhong Sun
International Journal of Biological Macromolecules.2018; 109: 872. CrossRef
Immobilization of Aspergillus awamori β-glucosidase on commercial gelatin: An inexpensive and efficient process
Verônica S. Nishida, Roselene F. de Oliveira, Tatiane Brugnari, Rúbia Carvalho G. Correa, Rosely A. Peralta, Rafael Castoldi, Cristina G.M. de Souza, Adelar Bracht, Rosane M. Peralta
International Journal of Biological Macromolecules.2018; 111: 1206. CrossRef
Glucose tolerant and glucose stimulated β-glucosidases – A review
José Carlos Santos Salgado, Luana Parras Meleiro, Sibeli Carli, Richard John Ward
Bioresource Technology.2018; 267: 704. CrossRef
Enhancing the Thermostability of Highly Active and Glucose-Tolerant β-Glucosidase Ks5A7 by Directed Evolution for Good Performance of Three Properties
Lichuang Cao, Shuifeng Li, Xin Huang, Zongmin Qin, Wei Kong, Wei Xie, Yuhuan Liu
Journal of Agricultural and Food Chemistry.2018; 66(50): 13228. CrossRef
When substrate inhibits and inhibitor activates: implications of β-glucosidases
Silja Kuusk, Priit Väljamäe
Biotechnology for Biofuels.2017;[Epub] CrossRef
Increased enzymatic hydrolysis of sugarcane bagasse by a novel glucose- and xylose-stimulated β-glucosidase from Anoxybacillus flavithermus subsp. yunnanensis E13T
Yang Liu, Rui Li, Jing Wang, Xiaohan Zhang, Rong Jia, Yi Gao, Hui Peng
BMC Biochemistry.2017;[Epub] CrossRef
Bioprospecting of novel thermostable β-glucosidase from Bacillus subtilis RA10 and its application in biomass hydrolysis
Rameshwar Tiwari, Puneet Kumar Singh, Surender Singh, Pawan K. S. Nain, Lata Nain, Pratyoosh Shukla
Biotechnology for Biofuels.2017;[Epub] CrossRef
Engineering the GH1 β-glucosidase from Humicola insolens: Insights on the stimulation of activity by glucose and xylose
Luana Parras Meleiro, José Carlos Santos Salgado, Raquel Fonseca Maldonado, Sibeli Carli, Luiz Alberto Beraldo Moraes, Richard John Ward, João Atílio Jorge, Rosa Prazeres Melo Furriel, Jong-Seong Jeon
PLOS ONE.2017; 12(11): e0188254. CrossRef
Two‐step statistical optimization for cold active β‐glucosidase production from Pseudomonas lutea BG8 and its application for improving saccharification of paddy straw
Rameshwar Tiwari, Kumar Pranaw, Surender Singh, Pawan K.S. Nain, Pratyoosh Shukla, Lata Nain
Biotechnology and Applied Biochemistry.2016; 63(5): 659. CrossRef
Improvements in Glucose Sensitivity and Stability of Trichoderma reesei β-Glucosidase Using Site-Directed Mutagenesis
Boyang Guo, Yoshihiko Amano, Kouichi Nozaki, Israel Silman
PLOS ONE.2016; 11(1): e0147301. CrossRef
[Review: Symposium on Applied Glycoscience] Function of β-Glucosidases in Cellulase Induction of Trichoderma reesei
Kouichi Nozaki, Boyang Guo, Satoshi Hatanaka, Peter Biely, Yoshihiko Amano
Bulletin of Applied Glycoscience.2016; 6(2): 96. CrossRef
Production and characterization of fungal β-glucosidase and bacterial cellulases by tobacco chloroplast transformation
Edward Alexander Espinoza-Sánchez, Jorge Ariel Torres-Castillo, Quintín Rascón-Cruz, Francisco Zavala-García, Sugey Ramona Sinagawa-García
Plant Biotechnology Reports.2016; 10(2): 61. CrossRef
Effect of pH, Temperature, and Chemicals on the Endoglucanases andβ-Glucosidases from the Thermophilic FungusMyceliophthora heterothallicaF.2.1.4. Obtained by Solid-State and Submerged Cultivation
Vanessa de Cássia Teixeira da Silva, Amanda Lais de Souza Coto, Rafael de Carvalho Souza, Marcello Bertoldi Sanchez Neves, Eleni Gomes, Gustavo Orlando Bonilla-Rodriguez
Biochemistry Research International.2016; 2016: 1. CrossRef
Passion fruit peel as novel substrate for enhanced β-glucosidases production by Penicillium verruculosum: Potential of the crude extract for biomass hydrolysis
J.M. Almeida, V.A. Lima, P.C. Giloni-Lima, A. Knob
Biomass and Bioenergy.2015; 72: 216. CrossRef
Engineering a novel glucose-tolerant β-glucosidase as supplementation to enhance the hydrolysis of sugarcane bagasse at high glucose concentration
Li-chuang Cao, Zhi-jun Wang, Guang-hui Ren, Wei Kong, Liang Li, Wei Xie, Yu-huan Liu
Biotechnology for Biofuels.2015;[Epub] CrossRef
A Neurospora crassa ÿ-glucosidase with potential for lignocellulose hydrolysis shows strong glucose tolerance and stimulation by glucose and xylose
Luana Parras Meleiro, José Carlos Santos Salgado, Raquel Fonseca Maldonado, Juliana Sanchez Alponti, Ana Lucia Ribeiro Latorre Zimbardi, João Atílio Jorge, Richard John Ward, Rosa Prazeres Melo Furriel
Journal of Molecular Catalysis B: Enzymatic.2015; 122: 131. CrossRef
Characterization of a novel Aspergillus niger beta-glucosidase tolerant to saccharification of lignocellulosic biomass products and fermentation inhibitors
Alesandra Oriente, Robson Tramontina, Diandra de Andrades, Caroline Henn, Jose L. C. Silva, Rita C. G. Simão, Alexandre Maller, Maria de Lourdes T. M. Polizeli, Marina K. Kadowaki
Chemical Papers.2015;[Epub] CrossRef
Comparative analysis of three hyperthermophilic GH1 and GH3 family members with industrial potential
Junio Cota, Thamy L.R. Corrêa, André R.L. Damásio, José A. Diogo, Zaira B. Hoffmam, Wanius Garcia, Leandro C. Oliveira, Rolf A. Prade, Fabio M. Squina
New Biotechnology.2015; 32(1): 13. CrossRef
Identification of a fungus able to secrete enzymes that degrade regenerated cellulose films and analyses of its extracellular hydrolases
Lijun Zhao, Qiming Wang, Shanbai Xiong
Annals of Microbiology.2014; 64(3): 1041. CrossRef
Immobilization and high stability of an extracellular β-glucosidase from Aspergillus japonicus by ionic interactions
Tony Marcio da Silva, Benevides Costa Pessela, Jean Carlos Rodrigues da Silva, Matheus Sanitá Lima, João Atílio Jorge, José Manuel Guisan, Maria de Lourdes T.M. Polizeli
Journal of Molecular Catalysis B: Enzymatic.2014; 104: 95. CrossRef
A Novel β-Glucosidase from Humicola insolens with High Potential for Untreated Waste Paper Conversion to Sugars
Luana Parras Meleiro, Ana Lucia Ribeiro Latorre Zimbardi, Flavio Henrique Moreira Souza, Douglas Chodi Masui, Tony Marcio Silva, João Atilio Jorge, Rosa Prazeres Melo Furriel
Applied Biochemistry and Biotechnology.2014; 173(2): 391. CrossRef
Gene cloning, expression and biochemical characterization of a glucose- and xylose-stimulated β-glucosidase from Humicola insolens RP86
Flavio Henrique Moreira Souza, Luana Parras Meleiro, Carla Botelho Machado, Ana Lucia Ribeiro Latorre Zimbardi, Raquel Fonseca Maldonado, Tatiana Arruda Campos Brasil Souza, Douglas Chodi Masui, Mario Tyago Murakami, João Atilio Jorge, Richard John Ward,
Journal of Molecular Catalysis B: Enzymatic.2014; 106: 1. CrossRef
Production and secretion of a multifunctional ß-glucosidase by Humicola grisea var. thermoidea: effects of L-sorbose
Sandra Abrão Lazari, José Carlos Santos Salgado, Douglas Chodi Masui, Rosane Marina Peralta, João Atílio Jorge, Rosa Prazeres Melo Furriel
Annals of Microbiology.2014; 64(3): 1089. CrossRef
Proteome-Based Profiling of Hypercellulase-Producing Strains Developed Through Interspecific Protoplast Fusion Between Aspergillus nidulans and Aspergillus tubingensis
Baljit Kaur, Manju Sharma, Rohit Soni, H. S. Oberoi, B. S. Chadha
Applied Biochemistry and Biotechnology.2013; 169(2): 393. CrossRef
Glucose and xylose stimulation of a β-glucosidase from the thermophilic fungus Humicola insolens: A kinetic and biophysical study
Flavio Henrique Moreira Souza, Rodrigo Furriel Inocentes, Richard John Ward, João Atílio Jorge, Rosa Prazeres Melo Furriel
Journal of Molecular Catalysis B: Enzymatic.2013; 94: 119. CrossRef
Optimization of β-Glucosidase, β-Xylosidase and Xylanase Production by Colletotrichum graminicola under Solid-State Fermentation and Application in Raw Sugarcane Trash Saccharification
Ana Zimbardi, Cesar Sehn, Luana Meleiro, Flavio Souza, Douglas Masui, Monica Nozawa, Luis Guimarães, João Jorge, Rosa Furriel
International Journal of Molecular Sciences.2013; 14(2): 2875. CrossRef
Purification and biochemical characterization of glucose–cellobiose-tolerant cellulases from Scytalidium thermophilum
Jean Carlos Rodrigues Silva, Luis Henrique Souza Guimarães, José Carlos Santos Salgado, Rosa Prazeres Melo Furriel, Maria Lourdes T. M. Polizeli, José César Rosa, João Atilio Jorge
Folia Microbiologica.2013; 58(6): 561. CrossRef
Advancements and future directions in enzyme technology for biomass conversion
Zisheng Zhang, Adam A. Donaldson, Xiaoxun Ma
Biotechnology Advances.2012; 30(4): 913. CrossRef
Production of a xylose-stimulated β-glucosidase and a cellulase-free thermostable xylanase by the thermophilic fungus Humicola brevis var. thermoidea under solid state fermentation
Douglas Chodi Masui, Ana Lucia Ribeiro Latorre Zimbardi, Flávio Henrique Moreira Souza, Luis Henrique Souza Guimarães, Rosa Prazeres Melo Furriel, João Atílio Jorge
World Journal of Microbiology and Biotechnology.2012; 28(8): 2689. CrossRef
Heterologous expression and characterization of a glucose-stimulated β-glucosidase from the termite Neotermes koshunensis in Aspergillus oryzae
Cristiane Akemi Uchima, Gaku Tokuda, Hirofumi Watanabe, Katsuhiko Kitamoto, Manabu Arioka
Applied Microbiology and Biotechnology.2011; 89(6): 1761. CrossRef

Effect of Glycosylation on the Biochemical Properties of beta-Xylosidases from Aspergillus versicolor: Alexandre Favarin Somera , Marita Gimenez Pereira , Luis Henrique Souza Guimaraes , Maria de Lourdes Teixeira de Moraes Polizeli , Hector Francisco Terenzi , Rosa Prazeres Melo Furriel , Joao Atilio Jorge; J. Microbiol. 2009;47(3):270-276. Published online June 26, 2009; DOI: https://doi.org/10.1007/s12275-008-0286-9

834 View
0 Download
19 Crossref

Abstract PDF

Aspergillus versicolor grown on xylan or xylose produces two beta-xylosidases with differences in biochemical properties and degree of glycosylation. We investigated the alterations in the biochemical properties of these beta-xylosidases after deglycosylation with Endo-H or PNGase F. After deglycosylation, both enzymes migrated faster in PAGE or SDS-PAGE exhibiting the same Rf. Temperature optimum of xylan-induced and xylose-induced beta-xylosidases was 45oC and 40oC, respectively, and 35oC after deglycosylation. The xylan- induced enzyme was more active at acidic pH. After deglycosylation, both enzymes had the same pH optimum of 6.0. Thermal resistance at 55oC showed half-life of 15 min and 9 min for xylose- and xylan-induced enzymes, respectively. After deglycosylation, both enzymes exhibited half-lives of 7.5 min. Native enzymes exhibited different responses to ions, while deglycosylated enzymes exhibited identical responses. Limited proteolysis yielded similar polypeptide profiles for the deglycosylated enzymes, suggesting a common polypeptide core with differential glycosylation apparently responsible for their biochemical and biophysical differences.

Citations

Citations to this article as recorded by

Comparative genomics of Aspergillus nidulans and section Nidulantes
Sebastian Theobald, Tammi Vesth, Jane L. Nybo, Jens C. Frisvad, Inge Kjærbølling, Stephen Mondo, Kurt LaButti, Sajeet Haridas, Robert Riley, Alan A. Kuo, Asaf A. Salamov, Jasmyn Pangilinan, Anna Lipzen, Maxim Koriabine, Mi Yan, Kerrie Barry, Alicia Clum,
Current Research in Microbial Sciences.2025; 8: 100342. CrossRef
Strategies for tailoring pH performances of glycoside hydrolases
Shu-Fang Li, Feng Cheng, Ya-Jun Wang, Yu-Guo Zheng
Critical Reviews in Biotechnology.2023; 43(1): 121. CrossRef
β-Xylosidase SRBX1 Activity from Sporisorium reilianum and Its Synergism with Xylanase SRXL1 in Xylose Release from Corn Hemicellulose
Yuridia Mercado-Flores, Alejandro Téllez-Jurado, Carlos Iván Lopéz-Gil, Miguel Angel Anducho-Reyes
Journal of Fungi.2022; 8(12): 1295. CrossRef
Stabilization of Glycosylated β-Glucosidase by Intramolecular Crosslinking Between Oxidized Glycosidic Chains and Lysine Residues
Laura Marina Pinotti, Paulo Waldir Tardioli, Cristiane Sanchez Farinas, Gloria Fernández-Lorente, Alejandro H. Orrego, Jose M. Guisan, Benevides C. Pessela
Applied Biochemistry and Biotechnology.2020; 192(1): 325. CrossRef
High cellulolytic activities in filamentous fungi isolated from an extreme oligotrophic subterranean environment (Catão cave) in Brazil
CAIO C.P. DE PAULA, QUIMI V. MONTOYA, LUCAS A. MEIRELLES, CRISTIANE S. FARINAS, ANDRÉ RODRIGUES, MIRNA H.R. SELEGHIM
Anais da Academia Brasileira de Ciências.2019;[Epub] CrossRef
Purification and Characterisation of a Thermostable β-Xylosidase from Aspergillus niger van Tieghem of Potential Application in Lignocellulosic Bioethanol Production
Angela Boyce, Gary Walsh
Applied Biochemistry and Biotechnology.2018; 186(3): 712. CrossRef
Immobilization of Aspergillus awamori β-glucosidase on commercial gelatin: An inexpensive and efficient process
Verônica S. Nishida, Roselene F. de Oliveira, Tatiane Brugnari, Rúbia Carvalho G. Correa, Rosely A. Peralta, Rafael Castoldi, Cristina G.M. de Souza, Adelar Bracht, Rosane M. Peralta
International Journal of Biological Macromolecules.2018; 111: 1206. CrossRef
Expression and Characterization of the RKOD DNA Polymerase in Pichia pastoris
Fei Wang, Shuntang Li, Hui Zhao, Lu Bian, Liang Chen, Zhen Zhang, Xing Zhong, Lixin Ma, Xiaolan Yu, Eugene A. Permyakov
PLOS ONE.2015; 10(7): e0131757. CrossRef
Gene cloning, expression and biochemical characterization of a glucose- and xylose-stimulated β-glucosidase from Humicola insolens RP86
Flavio Henrique Moreira Souza, Luana Parras Meleiro, Carla Botelho Machado, Ana Lucia Ribeiro Latorre Zimbardi, Raquel Fonseca Maldonado, Tatiana Arruda Campos Brasil Souza, Douglas Chodi Masui, Mario Tyago Murakami, João Atilio Jorge, Richard John Ward,
Journal of Molecular Catalysis B: Enzymatic.2014; 106: 1. CrossRef
N-glycosylation enhances functional and structural stability of recombinant β-glucuronidase expressed in Pichia pastoris
Shuping Zou, Shen Huang, Imdad Kaleem, Chun Li
Journal of Biotechnology.2013; 164(1): 75. CrossRef
Immobilization and biochemical properties of a β-xylosidase activated by glucose/xylose from Aspergillus niger USP-67 with transxylosylation activity
Vivian Machado Benassi, Tony Marcio da Silva, Benevides Costa Pessela, José Manuel Guisan, Cesar Mateo, Matheus Sanitá Lima, João Atílio Jorge, Maria de Lourdes T.M. Polizeli
Journal of Molecular Catalysis B: Enzymatic.2013; 89: 93. CrossRef
N-linked glycosylation influences on the catalytic and biochemical properties of Penicillium purpurogenum β-d-glucuronidase
Shuping Zou, Luping Xie, Yanli Liu, Imdad Kaleem, Guifeng Zhang, Chun Li
Journal of Biotechnology.2012; 157(3): 399. CrossRef
Functional characterization and synergic action of fungal xylanase and arabinofuranosidase for production of xylooligosaccharides
T.A. Gonçalves, A.R.L. Damásio, F. Segato, T.M. Alvarez, J. Bragatto, L.B. Brenelli, A.P.S. Citadini, M.T. Murakami, R. Ruller, A.F. Paes Leme, R.A. Prade, F.M. Squina
Bioresource Technology.2012; 119: 293. CrossRef
Production and action of an Aspergillus phoenicis enzymatic pool using different carbon sources
Vivian Machado Benassi, Rosymar Coutinho de Lucas, Michele Michelin, João Atílio Jorge, Héctor Francisco Terenzi, Maria de Lourdes Teixeira de Moraes Polizeli
Brazilian Journal of Food Technology.2012; 15(3): 253. CrossRef
The Combination of Lectin Affinity Chromatography, Gel Electrophoresis and Mass Spectrometry in the Study of Plant Glycoproteome: Preliminary Insights
Markéta Laštovičková, Dagmar Smětalová, Janette Bobalova
Chromatographia.2011; 73(S1): 113. CrossRef
β-Xylosidases from filamentous fungi: an overview
A. Knob, C. R. F. Terrasan, E. C. Carmona
World Journal of Microbiology and Biotechnology.2010; 26(3): 389. CrossRef
Purification and biochemical properties of a glucose-stimulated β-D-glucosidase produced by Humicola grisea var. thermoidea grown on sugarcane bagasse
Cesar Vanderlei Nascimento, Flávio Henrique Moreira Souza, Douglas Chodi Masui, Francisco Assis Leone, Rosane Marina Peralta, João Atílio Jorge, Rosa Prazeres Melo Furriel
The Journal of Microbiology.2010; 48(1): 53. CrossRef
Expression of an extremely acidic β-1,4-glucanase from thermoacidophilic Alicyclobacillus sp. A4 in Pichia pastoris is improved by truncating the gene sequence
Yingguo Bai, Jianshe Wang, Zhifang Zhang, Pengjun Shi, Huiying Luo, Huoqing Huang, Chunliang Luo, Bin Yao
Microbial Cell Factories.2010;[Epub] CrossRef
Extremely Acidic β-1,4-Glucanase, CelA4, from Thermoacidophilic Alicyclobacillus sp. A4 with High Protease Resistance and Potential as a Pig Feed Additive
Yingguo Bai, Jianshe Wang, Zhifang Zhang, Pengjun Shi, Huiying Luo, Huoqing Huang, Yukun Feng, Bin Yao
Journal of Agricultural and Food Chemistry.2010; 58(3): 1970. CrossRef

Author index