- Molecular characterization of Hsf1 as a master regulator of heat shock response in the thermotolerant methylotrophic yeast Ogataea parapolymorpha
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Jin Ho Choo , Su-Bin Lee , Hye Yun Moon , Kun Hwa Lee , Su Jin Yoo , Keun Pil Kim , Hyun Ah Kang
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J. Microbiol. 2021;59(2):151-163. Published online February 1, 2021
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DOI: https://doi.org/10.1007/s12275-021-0646-2
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Ogataea parapolymorpha (Hansenula polymorpha DL-1) is
a thermotolerant methylotrophic yeast with biotechnological
applications. Here, O. parapolymorpha genes whose expression
is induced in response to heat shock were identified by
transcriptome analysis and shown to possess heat shock elements
(HSEs) in their promoters. The function of O. parapolymorpha
HSF1 encoding a putative heat shock transcription
factor 1 (OpHsf1) was characterized in the context of heat
stress response. Despite exhibiting low sequence identity
(26%) to its Saccharomyces cerevisiae homolog, OpHsf1 harbors
conserved domains including a DNA binding domain
(DBD), domains involved in trimerization (TRI), transcriptional
activation (AR1, AR2), transcriptional repression (CE2),
and a C-terminal modulator (CTM) domain. OpHSF1 could
complement the temperature sensitive (Ts) phenotype of a
S. cerevisiae hsf1 mutant. An O. parapolymorpha strain with
an H221R mutation in the DBD domain of OpHsf1 exhibited
significantly retarded growth and a Ts phenotype. Intriguingly,
the expression of heat-shock-protein‒coding genes harboring
HSEs was significantly decreased in the H221R mutant
strain, even under non-stress conditions, indicating the importance
of the DBD for the basal growth of O. parapolymorpha.
Notably, even though the deletion of C-terminal domains
(ΔCE2, ΔAR2, ΔCTM) of OpHsf1 destroyed complementation
of the growth defect of the S. cerevisiae hsf1 strain,
the C-terminal domains were shown to be dispensable in O.
parapolymorpha. Overexpression of OpHsf1 in S. cerevisiae
increased resistance to transient heat shock, supporting the
idea that OpHsf1 could be useful in the development of heatshock‒
resistant yeast host strains.
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Citations
Citations to this article as recorded by  - Thermotolerant yeasts and their biotechnological applications
Roksolana Vasylyshyn, Justyna Ruchala, Kostyantyn Dmytruk, Andriy Sibirny
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Somsak Likhitrattanapisal, Chitwadee Phithakrotchanakoon, Aekkachai Puseenam, Paopit Siriarchawatana, Natta Wiriyakun, Jiraprapa Nirapun, Warasirin Sornlek, Supawadee Ingsriswang, Niran Roongsawang
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Frontiers in Bioengineering and Biotechnology.2023;[Epub] CrossRef - Heat shock in Cronobacter sakazakii induces direct protection and cross-protection against simulated gastric fluid stress
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- Functional analysis of Mpk1-mediated cell wall integrity signaling pathway in the thermotolerant methylotrophic yeast Hansenula polymorpha
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Hyunah Kim , Eun Jung Thak , Ji Yoon Yeon , Min Jeong Sohn , Jin Ho Choo , Jeong-Yoon Kim , Hyun Ah Kang
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J. Microbiol. 2018;56(1):72-82. Published online January 4, 2018
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DOI: https://doi.org/10.1007/s12275-018-7508-6
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Abstract
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Understanding the characteristics and regulation mechanisms
of cell wall integrity (CWI) in yeast is important not
only for basic research but also in biotechnological applications.
We found significantly different CWIs in two representative
strains of the thermotolerant methylotrophic yeast
Hansenula polymorpha. Compared to the A16 strain (classified
as Ogataea polymorpha), the DL1-L strain (classified as
Ogataea parapolymorpha) has a thinner cell wall that was
found to be more fragile following long-term cultivation and
more sensitive to zymolyase. To gain a deeper insight into this
difference, we compared the characteristics of the Mpk1pmediated
CWI signaling pathway in the two strains. While
a DL1-L mutant deficient in Mpk1p (mpk1Δ) showed severe
growth retardation at both normal and high growth temperatures
and in the presence of cell-wall disrupting agents, the
A16 mpk1Δ mutant displayed only a mild defect in cell growth.
Sorbitol effect on rescuing growth retardation was different
in the two mpk1Δ strains, which could partly be ascribed to
subtle differences in the activation of HOG pathway. Among
the cell wall disruptors evaluated, only caffeine clearly increased
phosphorylation of Mpk1p in DL1-L, but not in A16.
A transcriptome analysis of the DL1-L strain revealed that
caffeine significantly increased the expression of a subset of
cell-wall related genes in an Mpk1p-dependent manner, but
not the expected Rlm1-target genes. Taken together, our data
support an essential role for Mpk1p in maintaining CWI in
H. polymorpha, although the requirement for Mpk1p and
its regulation under diverse stress conditions varies depending
on the strain background.
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Citations
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Ricardo A. Ribeiro, Nuno Bourbon-Melo, Isabel Sá-Correia
Frontiers in Microbiology.2022;[Epub] CrossRef - The damage and tolerance mechanisms of Phaffia rhodozyma mutant strain MK19 grown at 28 °C
Li-Li Miao, Shuang Chi, Ting-Ting Hou, Zhi-Pei Liu, Ying Li
Microbial Cell Factories.2021;[Epub] CrossRef - Molecular characterization of Hsf1 as a master regulator of heat shock response in the thermotolerant methylotrophic yeast Ogataea parapolymorpha
Jin Ho Choo, Su-Bin Lee, Hye Yun Moon, Kun Hwa Lee, Su Jin Yoo, Keun Pil Kim, Hyun Ah Kang
Journal of Microbiology.2021; 59(2): 151. CrossRef - Pleiotropic Effects of Caffeine Leading to Chromosome Instability and Cytotoxicity in Eukaryotic Microorganisms
Woo-Hyun Chung
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Su Jin Yoo, Min Jeong Sohn, Da Min Jeong, Hyun Ah Kang
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